Analysis of hybrids of durum wheat with Thinopyrum junceiforme using RAPD markers
The objective of this study was to detect the presence of alien chromatin in intergeneric hybrids of durum wheat (Triticum turgidum, 2n = 4x = 28; AABB genomes) with the perennial grass Thinopyrum junceiforme (2n = 4x = 28; J1J1J2J2) using RAPD markers. The first step was to identify amplification o...
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| Veröffentlicht in: | Theoretical and applied genetics 1997-10, Vol.95 (5/6), p.757-763 |
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| creator | Bommineni, V.R Jauhar, P.P Peterson, T.S Chibbar, R.N Almouslem, A.B |
| description | The objective of this study was to detect the presence of alien chromatin in intergeneric hybrids of durum wheat (Triticum turgidum, 2n = 4x = 28; AABB genomes) with the perennial grass Thinopyrum junceiforme (2n = 4x = 28; J1J1J2J2) using RAPD markers. The first step was to identify amplification of species-specific DNA markers in the parental grass species and durum wheat cultivars. Initially, the genomic DNA of five grass species (Thinopyrum junceiforme, Th. bessarabicum, Lophopyrum elongatum, Leymus karataviensis and Elytrigia pycnantha) and selected durum cultivars ('Langdon', 'Durox', 'Lloyd', 'Monroe', and 'Medora') was screened with 40 oligonucleotide primers (nano-mers). Three oligonucleotides that amplified DNA fragments specific to a grass species or to a durum cultivar were identified. Primer PR21 amplified DNA fragments specific to each of the five durum cultivars, and primers PR22 and PR23 amplified fragments specific to each of the grass species. Intergeneric hybrids between the durum cultivars 'Langdon', 'Lloyd' and 'Durox' and Th. junceiforme, and their backcross (BC) progeny were screened with all 40 primers. Six primers amplified parent-specific DNA fragments in the F1 hybrids and their BC1 progeny. Three primers, PR22, PR23 and PR41, that amplified Th. junceiforme DNA fragments in both F1 and BC1 were further analyzed. The presence of an amplified 1.7-kb Th. junceiforme DNA fragment in the F1 hybrids and BC1 progeny was confirmed using Southern analysis by hybridization with both Th. junceiforme genomic DNA and Th. junceiforme DNA amplified with primer PR41. With the exception of line BC1F2 no. 5. five selfed progeny of BC1 and a BC2 of line 3 (BC1F2 no. 3 x'Lloyd') from a cross of 'Lloyd' x Th. junceiforme showed the presence of the 1.7-kb DNA fragment. All selfed BC1 and BC2 lines retained the 600-bp fragment that was confirmed after hybridization with Th. junceiforme DNA amplified with primer PR22. Other experiments using RFLP markers also showed the presence of up to seven Th. junceiforme DNA fragments in the F1 hybrids and their BC progeny after hybridization with Th. junceiforme DNA amplified with primer PR41. These studies show the usefulness of molecular markers in detecting alien chromatin/DNA fragments in intergeneric hybrids with durum wheat. |
| doi_str_mv | 10.1007/s001220050622 |
| format | Article |
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The first step was to identify amplification of species-specific DNA markers in the parental grass species and durum wheat cultivars. Initially, the genomic DNA of five grass species (Thinopyrum junceiforme, Th. bessarabicum, Lophopyrum elongatum, Leymus karataviensis and Elytrigia pycnantha) and selected durum cultivars ('Langdon', 'Durox', 'Lloyd', 'Monroe', and 'Medora') was screened with 40 oligonucleotide primers (nano-mers). Three oligonucleotides that amplified DNA fragments specific to a grass species or to a durum cultivar were identified. Primer PR21 amplified DNA fragments specific to each of the five durum cultivars, and primers PR22 and PR23 amplified fragments specific to each of the grass species. Intergeneric hybrids between the durum cultivars 'Langdon', 'Lloyd' and 'Durox' and Th. junceiforme, and their backcross (BC) progeny were screened with all 40 primers. Six primers amplified parent-specific DNA fragments in the F1 hybrids and their BC1 progeny. Three primers, PR22, PR23 and PR41, that amplified Th. junceiforme DNA fragments in both F1 and BC1 were further analyzed. The presence of an amplified 1.7-kb Th. junceiforme DNA fragment in the F1 hybrids and BC1 progeny was confirmed using Southern analysis by hybridization with both Th. junceiforme genomic DNA and Th. junceiforme DNA amplified with primer PR41. With the exception of line BC1F2 no. 5. five selfed progeny of BC1 and a BC2 of line 3 (BC1F2 no. 3 x'Lloyd') from a cross of 'Lloyd' x Th. junceiforme showed the presence of the 1.7-kb DNA fragment. All selfed BC1 and BC2 lines retained the 600-bp fragment that was confirmed after hybridization with Th. junceiforme DNA amplified with primer PR22. Other experiments using RFLP markers also showed the presence of up to seven Th. junceiforme DNA fragments in the F1 hybrids and their BC progeny after hybridization with Th. junceiforme DNA amplified with primer PR41. These studies show the usefulness of molecular markers in detecting alien chromatin/DNA fragments in intergeneric hybrids with durum wheat.</description><identifier>ISSN: 0040-5752</identifier><identifier>EISSN: 1432-2242</identifier><identifier>DOI: 10.1007/s001220050622</identifier><identifier>CODEN: THAGA6</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>backcrossing ; Biological and medical sciences ; Biological markers ; Chromatin ; Classical genetics, quantitative genetics, hybrids ; Cultivars ; Deoxyribonucleic acid ; DNA ; DNA primers ; Durum wheat ; Elymus ; Elytrigia ; Fundamental and applied biological sciences. Psychology ; Gene amplification ; Genetic aspects ; genetic markers ; Genetics ; Genetics of eukaryotes. Biological and molecular evolution ; Genomics ; Hybridization, Vegetable ; hybrids ; Identification and classification ; intergeneric hybridization ; Leymus ; leymus karataviensis ; Plant genetics ; Poaceae ; polymerase chain reaction ; Pteridophyta, spermatophyta ; random amplified polymorphic DNA technique ; restriction fragment length polymorphism ; Southern blotting ; Thinopyrum bessarabicum ; Thinopyrum elongatum ; Thinopyrum pycnanthum ; Triticum aestivum ; Triticum turgidum ; Vegetals ; Wheat</subject><ispartof>Theoretical and applied genetics, 1997-10, Vol.95 (5/6), p.757-763</ispartof><rights>1998 INIST-CNRS</rights><rights>COPYRIGHT 1997 Springer</rights><rights>Springer-Verlag Berlin Heidelberg 1997</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c440t-99a5a8145f6ed44b5c3da1419118539bb574b783bd641d3e1b5579b314be52e33</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2049092$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Bommineni, V.R</creatorcontrib><creatorcontrib>Jauhar, P.P</creatorcontrib><creatorcontrib>Peterson, T.S</creatorcontrib><creatorcontrib>Chibbar, R.N</creatorcontrib><creatorcontrib>Almouslem, A.B</creatorcontrib><title>Analysis of hybrids of durum wheat with Thinopyrum junceiforme using RAPD markers</title><title>Theoretical and applied genetics</title><description>The objective of this study was to detect the presence of alien chromatin in intergeneric hybrids of durum wheat (Triticum turgidum, 2n = 4x = 28; AABB genomes) with the perennial grass Thinopyrum junceiforme (2n = 4x = 28; J1J1J2J2) using RAPD markers. The first step was to identify amplification of species-specific DNA markers in the parental grass species and durum wheat cultivars. Initially, the genomic DNA of five grass species (Thinopyrum junceiforme, Th. bessarabicum, Lophopyrum elongatum, Leymus karataviensis and Elytrigia pycnantha) and selected durum cultivars ('Langdon', 'Durox', 'Lloyd', 'Monroe', and 'Medora') was screened with 40 oligonucleotide primers (nano-mers). Three oligonucleotides that amplified DNA fragments specific to a grass species or to a durum cultivar were identified. Primer PR21 amplified DNA fragments specific to each of the five durum cultivars, and primers PR22 and PR23 amplified fragments specific to each of the grass species. Intergeneric hybrids between the durum cultivars 'Langdon', 'Lloyd' and 'Durox' and Th. junceiforme, and their backcross (BC) progeny were screened with all 40 primers. Six primers amplified parent-specific DNA fragments in the F1 hybrids and their BC1 progeny. Three primers, PR22, PR23 and PR41, that amplified Th. junceiforme DNA fragments in both F1 and BC1 were further analyzed. The presence of an amplified 1.7-kb Th. junceiforme DNA fragment in the F1 hybrids and BC1 progeny was confirmed using Southern analysis by hybridization with both Th. junceiforme genomic DNA and Th. junceiforme DNA amplified with primer PR41. With the exception of line BC1F2 no. 5. five selfed progeny of BC1 and a BC2 of line 3 (BC1F2 no. 3 x'Lloyd') from a cross of 'Lloyd' x Th. junceiforme showed the presence of the 1.7-kb DNA fragment. All selfed BC1 and BC2 lines retained the 600-bp fragment that was confirmed after hybridization with Th. junceiforme DNA amplified with primer PR22. Other experiments using RFLP markers also showed the presence of up to seven Th. junceiforme DNA fragments in the F1 hybrids and their BC progeny after hybridization with Th. junceiforme DNA amplified with primer PR41. These studies show the usefulness of molecular markers in detecting alien chromatin/DNA fragments in intergeneric hybrids with durum wheat.</description><subject>backcrossing</subject><subject>Biological and medical sciences</subject><subject>Biological markers</subject><subject>Chromatin</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>Cultivars</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA primers</subject><subject>Durum wheat</subject><subject>Elymus</subject><subject>Elytrigia</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene amplification</subject><subject>Genetic aspects</subject><subject>genetic markers</subject><subject>Genetics</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Genomics</subject><subject>Hybridization, Vegetable</subject><subject>hybrids</subject><subject>Identification and classification</subject><subject>intergeneric hybridization</subject><subject>Leymus</subject><subject>leymus karataviensis</subject><subject>Plant genetics</subject><subject>Poaceae</subject><subject>polymerase chain reaction</subject><subject>Pteridophyta, spermatophyta</subject><subject>random amplified polymorphic DNA technique</subject><subject>restriction fragment length polymorphism</subject><subject>Southern blotting</subject><subject>Thinopyrum bessarabicum</subject><subject>Thinopyrum elongatum</subject><subject>Thinopyrum pycnanthum</subject><subject>Triticum aestivum</subject><subject>Triticum turgidum</subject><subject>Vegetals</subject><subject>Wheat</subject><issn>0040-5752</issn><issn>1432-2242</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp90ctvEzEQB2ALUYlQOHJmBQjEYWHGj13vMSqvSpWAPs6W7fUmDpt1sHdV8t_XSapK6YGTLevzaOY3hLxC-IQA9ecEgJQCCKgofUJmyBktKeX0KZkBcChFLegz8jylFQBQAWxGfs8H3W-TT0XoiuXWRN_ur-0Up3Vxu3R6LG79uCyul34Im-3udTUN1vkuxLUrpuSHRXE5__WlWOv4x8X0gpx0uk_u5f15Sm6-fb0--1Fe_Px-fja_KC3nMJZNo4WWyEVXuZZzIyxrNXJsEKVgjTGi5qaWzLQVx5Y5NELUjWHIjRPUMXZKPhzqbmL4O7k0qrVP1vW9HlyYkmoqiRJ4Q7N8_1-JFReVrDDDN4_gKkwxB5SUFLkxirLK6O0BLXTvlB-6MEZtdxXVnAE0dcP3pT4eKRuG0f0bF3pKSZ1fXR7b8mBtDClF16lN9DnNrUJQu82qo81m_-6-TZ2s7ruoB-vTwyeah4b92K8PrNNB6UXM5OaKAjKgMgcDlN0BwA6ncg</recordid><startdate>19971001</startdate><enddate>19971001</enddate><creator>Bommineni, V.R</creator><creator>Jauhar, P.P</creator><creator>Peterson, T.S</creator><creator>Chibbar, R.N</creator><creator>Almouslem, A.B</creator><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7SS</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope></search><sort><creationdate>19971001</creationdate><title>Analysis of hybrids of durum wheat with Thinopyrum junceiforme using RAPD markers</title><author>Bommineni, V.R ; Jauhar, P.P ; Peterson, T.S ; Chibbar, R.N ; Almouslem, A.B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c440t-99a5a8145f6ed44b5c3da1419118539bb574b783bd641d3e1b5579b314be52e33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>backcrossing</topic><topic>Biological and medical sciences</topic><topic>Biological markers</topic><topic>Chromatin</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>Cultivars</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA primers</topic><topic>Durum wheat</topic><topic>Elymus</topic><topic>Elytrigia</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene amplification</topic><topic>Genetic aspects</topic><topic>genetic markers</topic><topic>Genetics</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Genomics</topic><topic>Hybridization, Vegetable</topic><topic>hybrids</topic><topic>Identification and classification</topic><topic>intergeneric hybridization</topic><topic>Leymus</topic><topic>leymus karataviensis</topic><topic>Plant genetics</topic><topic>Poaceae</topic><topic>polymerase chain reaction</topic><topic>Pteridophyta, spermatophyta</topic><topic>random amplified polymorphic DNA technique</topic><topic>restriction fragment length polymorphism</topic><topic>Southern blotting</topic><topic>Thinopyrum bessarabicum</topic><topic>Thinopyrum elongatum</topic><topic>Thinopyrum pycnanthum</topic><topic>Triticum aestivum</topic><topic>Triticum turgidum</topic><topic>Vegetals</topic><topic>Wheat</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bommineni, V.R</creatorcontrib><creatorcontrib>Jauhar, P.P</creatorcontrib><creatorcontrib>Peterson, T.S</creatorcontrib><creatorcontrib>Chibbar, R.N</creatorcontrib><creatorcontrib>Almouslem, A.B</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><jtitle>Theoretical and applied genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bommineni, V.R</au><au>Jauhar, P.P</au><au>Peterson, T.S</au><au>Chibbar, R.N</au><au>Almouslem, A.B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of hybrids of durum wheat with Thinopyrum junceiforme using RAPD markers</atitle><jtitle>Theoretical and applied genetics</jtitle><date>1997-10-01</date><risdate>1997</risdate><volume>95</volume><issue>5/6</issue><spage>757</spage><epage>763</epage><pages>757-763</pages><issn>0040-5752</issn><eissn>1432-2242</eissn><coden>THAGA6</coden><abstract>The objective of this study was to detect the presence of alien chromatin in intergeneric hybrids of durum wheat (Triticum turgidum, 2n = 4x = 28; AABB genomes) with the perennial grass Thinopyrum junceiforme (2n = 4x = 28; J1J1J2J2) using RAPD markers. The first step was to identify amplification of species-specific DNA markers in the parental grass species and durum wheat cultivars. Initially, the genomic DNA of five grass species (Thinopyrum junceiforme, Th. bessarabicum, Lophopyrum elongatum, Leymus karataviensis and Elytrigia pycnantha) and selected durum cultivars ('Langdon', 'Durox', 'Lloyd', 'Monroe', and 'Medora') was screened with 40 oligonucleotide primers (nano-mers). Three oligonucleotides that amplified DNA fragments specific to a grass species or to a durum cultivar were identified. Primer PR21 amplified DNA fragments specific to each of the five durum cultivars, and primers PR22 and PR23 amplified fragments specific to each of the grass species. Intergeneric hybrids between the durum cultivars 'Langdon', 'Lloyd' and 'Durox' and Th. junceiforme, and their backcross (BC) progeny were screened with all 40 primers. Six primers amplified parent-specific DNA fragments in the F1 hybrids and their BC1 progeny. Three primers, PR22, PR23 and PR41, that amplified Th. junceiforme DNA fragments in both F1 and BC1 were further analyzed. The presence of an amplified 1.7-kb Th. junceiforme DNA fragment in the F1 hybrids and BC1 progeny was confirmed using Southern analysis by hybridization with both Th. junceiforme genomic DNA and Th. junceiforme DNA amplified with primer PR41. With the exception of line BC1F2 no. 5. five selfed progeny of BC1 and a BC2 of line 3 (BC1F2 no. 3 x'Lloyd') from a cross of 'Lloyd' x Th. junceiforme showed the presence of the 1.7-kb DNA fragment. All selfed BC1 and BC2 lines retained the 600-bp fragment that was confirmed after hybridization with Th. junceiforme DNA amplified with primer PR22. Other experiments using RFLP markers also showed the presence of up to seven Th. junceiforme DNA fragments in the F1 hybrids and their BC progeny after hybridization with Th. junceiforme DNA amplified with primer PR41. These studies show the usefulness of molecular markers in detecting alien chromatin/DNA fragments in intergeneric hybrids with durum wheat.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><doi>10.1007/s001220050622</doi><tpages>7</tpages></addata></record> |
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| ispartof | Theoretical and applied genetics, 1997-10, Vol.95 (5/6), p.757-763 |
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| source | SpringerNature Journals |
| subjects | backcrossing Biological and medical sciences Biological markers Chromatin Classical genetics, quantitative genetics, hybrids Cultivars Deoxyribonucleic acid DNA DNA primers Durum wheat Elymus Elytrigia Fundamental and applied biological sciences. Psychology Gene amplification Genetic aspects genetic markers Genetics Genetics of eukaryotes. Biological and molecular evolution Genomics Hybridization, Vegetable hybrids Identification and classification intergeneric hybridization Leymus leymus karataviensis Plant genetics Poaceae polymerase chain reaction Pteridophyta, spermatophyta random amplified polymorphic DNA technique restriction fragment length polymorphism Southern blotting Thinopyrum bessarabicum Thinopyrum elongatum Thinopyrum pycnanthum Triticum aestivum Triticum turgidum Vegetals Wheat |
| title | Analysis of hybrids of durum wheat with Thinopyrum junceiforme using RAPD markers |
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