Identification of molecular markers linked to the Yr15 stripe rust resistance gene of wheat originated in wild emmer wheat, Triticum dicoccoides
The Yr15 gene of wheat confers resistance to the stripe rust pathogen Puccinia striiformis West., which is one of the most devastating diseases of wheat throughout the world. In the present study, molecular markers flanking the Yr15 gene of wheat have been identified using the near-isogenic-lines ap...
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| Veröffentlicht in: | Theoretical and applied genetics 1997-09, Vol.95 (4), p.622-628 |
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| creator | Sun, G.L Fahima, T Korol, A.B Turpeinen, T Grama, A Ronin, Y.I Nevo, E |
| description | The Yr15 gene of wheat confers resistance to the stripe rust pathogen Puccinia striiformis West., which is one of the most devastating diseases of wheat throughout the world. In the present study, molecular markers flanking the Yr15 gene of wheat have been identified using the near-isogenic-lines approach. RFLP screening of 76 probe-enzyme combinations revealed one polymorphic marker (Nor/TaqI) between the susceptible and the resistant lines. In addition, out of 340 RAPD primers tested, six produced polymorphic RAPD bands between the susceptible and the resistant lines. The genetic linkage of the polymorphic markers was tested on segregating F2 population (123 plants) derived from crosses between stripe rust-susceptible Triticum durum wheat, cv D447, and a BC3F9 resistant line carrying Yr15 in a D447 background. A 2.8-kb fragment produced by the Nor RFLP probe and a 1420-bp PCR product generated by the RAPD primer OPB13 showed linkage, in coupling, with the Yr15 gene. Employing the standard maximum- likelihood technique it was found that the order OPB13(1420-) Yr15-Nor1 on chromosome 1B appeared to be no less than 1000-times more probable than the closest alternative. The map distances between OPB13(1420-) Yr15-Nor1 are 27.1 cM and 11.0 cM for the first and second intervals, respectively. The application of marker-assisted selection for the breeding of new wheat cultivars with the stripe rust resistance gene is discussed. |
| doi_str_mv | 10.1007/s001220050604 |
| format | Article |
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In the present study, molecular markers flanking the Yr15 gene of wheat have been identified using the near-isogenic-lines approach. RFLP screening of 76 probe-enzyme combinations revealed one polymorphic marker (Nor/TaqI) between the susceptible and the resistant lines. In addition, out of 340 RAPD primers tested, six produced polymorphic RAPD bands between the susceptible and the resistant lines. The genetic linkage of the polymorphic markers was tested on segregating F2 population (123 plants) derived from crosses between stripe rust-susceptible Triticum durum wheat, cv D447, and a BC3F9 resistant line carrying Yr15 in a D447 background. A 2.8-kb fragment produced by the Nor RFLP probe and a 1420-bp PCR product generated by the RAPD primer OPB13 showed linkage, in coupling, with the Yr15 gene. Employing the standard maximum- likelihood technique it was found that the order OPB13(1420-) Yr15-Nor1 on chromosome 1B appeared to be no less than 1000-times more probable than the closest alternative. The map distances between OPB13(1420-) Yr15-Nor1 are 27.1 cM and 11.0 cM for the first and second intervals, respectively. The application of marker-assisted selection for the breeding of new wheat cultivars with the stripe rust resistance gene is discussed.</description><identifier>ISSN: 0040-5752</identifier><identifier>EISSN: 1432-2242</identifier><identifier>DOI: 10.1007/s001220050604</identifier><identifier>CODEN: THAGA6</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Biological and medical sciences ; chromosome mapping ; Classical genetics, quantitative genetics, hybrids ; Crop diseases ; Development and progression ; disease resistance ; Diseases and pests ; Fundamental and applied biological sciences. Psychology ; genes ; Genetic aspects ; genetic markers ; Genetics ; Genetics of eukaryotes. Biological and molecular evolution ; Genomics ; inheritance (genetics) ; interspecific hybridization ; linkage (genetics) ; Molecular genetics ; Physiological aspects ; Plant diseases ; Plant genetics ; Plant immunology ; Pteridophyta, spermatophyta ; Puccinia striiformis ; random amplified polymorphic DNA technique ; restriction fragment length polymorphism ; rust diseases ; segregation ; Triticum aestivum ; Triticum dicoccoides ; Triticum dicoccum ; Triticum durum ; Triticum turgidum subsp. dicoccoides ; Triticum turgidum subsp. durum ; Vegetals ; Wheat ; yr15 gene</subject><ispartof>Theoretical and applied genetics, 1997-09, Vol.95 (4), p.622-628</ispartof><rights>1997 INIST-CNRS</rights><rights>COPYRIGHT 1997 Springer</rights><rights>Springer-Verlag Berlin Heidelberg 1997</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-a9474866c3047533d1ee87e8ac235885f136937466ca5eaaa034c4a6e92b21493</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2816833$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Sun, G.L</creatorcontrib><creatorcontrib>Fahima, T</creatorcontrib><creatorcontrib>Korol, A.B</creatorcontrib><creatorcontrib>Turpeinen, T</creatorcontrib><creatorcontrib>Grama, A</creatorcontrib><creatorcontrib>Ronin, Y.I</creatorcontrib><creatorcontrib>Nevo, E</creatorcontrib><title>Identification of molecular markers linked to the Yr15 stripe rust resistance gene of wheat originated in wild emmer wheat, Triticum dicoccoides</title><title>Theoretical and applied genetics</title><description>The Yr15 gene of wheat confers resistance to the stripe rust pathogen Puccinia striiformis West., which is one of the most devastating diseases of wheat throughout the world. In the present study, molecular markers flanking the Yr15 gene of wheat have been identified using the near-isogenic-lines approach. RFLP screening of 76 probe-enzyme combinations revealed one polymorphic marker (Nor/TaqI) between the susceptible and the resistant lines. In addition, out of 340 RAPD primers tested, six produced polymorphic RAPD bands between the susceptible and the resistant lines. The genetic linkage of the polymorphic markers was tested on segregating F2 population (123 plants) derived from crosses between stripe rust-susceptible Triticum durum wheat, cv D447, and a BC3F9 resistant line carrying Yr15 in a D447 background. A 2.8-kb fragment produced by the Nor RFLP probe and a 1420-bp PCR product generated by the RAPD primer OPB13 showed linkage, in coupling, with the Yr15 gene. Employing the standard maximum- likelihood technique it was found that the order OPB13(1420-) Yr15-Nor1 on chromosome 1B appeared to be no less than 1000-times more probable than the closest alternative. The map distances between OPB13(1420-) Yr15-Nor1 are 27.1 cM and 11.0 cM for the first and second intervals, respectively. The application of marker-assisted selection for the breeding of new wheat cultivars with the stripe rust resistance gene is discussed.</description><subject>Biological and medical sciences</subject><subject>chromosome mapping</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>Crop diseases</subject><subject>Development and progression</subject><subject>disease resistance</subject><subject>Diseases and pests</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genes</subject><subject>Genetic aspects</subject><subject>genetic markers</subject><subject>Genetics</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Genomics</subject><subject>inheritance (genetics)</subject><subject>interspecific hybridization</subject><subject>linkage (genetics)</subject><subject>Molecular genetics</subject><subject>Physiological aspects</subject><subject>Plant diseases</subject><subject>Plant genetics</subject><subject>Plant immunology</subject><subject>Pteridophyta, spermatophyta</subject><subject>Puccinia striiformis</subject><subject>random amplified polymorphic DNA technique</subject><subject>restriction fragment length polymorphism</subject><subject>rust diseases</subject><subject>segregation</subject><subject>Triticum aestivum</subject><subject>Triticum dicoccoides</subject><subject>Triticum dicoccum</subject><subject>Triticum durum</subject><subject>Triticum turgidum subsp. dicoccoides</subject><subject>Triticum turgidum subsp. durum</subject><subject>Vegetals</subject><subject>Wheat</subject><subject>yr15 gene</subject><issn>0040-5752</issn><issn>1432-2242</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp90U1rFTEUBuBBFLxWl64NIorg6MnXJLMsxY8LBcG2C1fhmDlzm3YmqUmG6r_wJzuXWwRduMriPOflhLdpnnJ4ywHMuwLAhQDQ0IG612y4kqIVQon7zQZAQauNFg-bR6VcAYDQIDfNr-1AsYYxeKwhRZZGNqeJ_DJhZjPma8qFTSFe08BqYvWS2NfMNSs1hxtieSmVZSqhVIye2I4i7TNuLwkrSznsQsS67obIbsM0MJpnyofxG3aeQw1-mdkQfPI-hYHK4-bBiFOhJ3fvUXPx4f35yaf29PPH7cnxaesVF7XFXhllu85LUEZLOXAia8iiF1Jbq0cuu14atQrUhIgglVfYUS--Ca56edS8OuTe5PR9oVLdHIqnacJIaSmu7yw3vQa1ypf_lbxTvDd6H_n8H3iVlhzXXzire84F7_dprw9ohxO5EH2KlX7UHS6luO3ZF3csATrNbS9X2x6sz6mUTKO7yWEt5afj4PaVu78qX_2LuwOweJzGvHYSyp8lYXln5T722YGNmBzu8kouzgRwCcIao0wnfwPc2LMn</recordid><startdate>19970901</startdate><enddate>19970901</enddate><creator>Sun, G.L</creator><creator>Fahima, T</creator><creator>Korol, A.B</creator><creator>Turpeinen, T</creator><creator>Grama, A</creator><creator>Ronin, Y.I</creator><creator>Nevo, E</creator><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7SS</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope></search><sort><creationdate>19970901</creationdate><title>Identification of molecular markers linked to the Yr15 stripe rust resistance gene of wheat originated in wild emmer wheat, Triticum dicoccoides</title><author>Sun, G.L ; Fahima, T ; Korol, A.B ; Turpeinen, T ; Grama, A ; Ronin, Y.I ; Nevo, E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-a9474866c3047533d1ee87e8ac235885f136937466ca5eaaa034c4a6e92b21493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Biological and medical sciences</topic><topic>chromosome mapping</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>Crop diseases</topic><topic>Development and progression</topic><topic>disease resistance</topic><topic>Diseases and pests</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genes</topic><topic>Genetic aspects</topic><topic>genetic markers</topic><topic>Genetics</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Genomics</topic><topic>inheritance (genetics)</topic><topic>interspecific hybridization</topic><topic>linkage (genetics)</topic><topic>Molecular genetics</topic><topic>Physiological aspects</topic><topic>Plant diseases</topic><topic>Plant genetics</topic><topic>Plant immunology</topic><topic>Pteridophyta, spermatophyta</topic><topic>Puccinia striiformis</topic><topic>random amplified polymorphic DNA technique</topic><topic>restriction fragment length polymorphism</topic><topic>rust diseases</topic><topic>segregation</topic><topic>Triticum aestivum</topic><topic>Triticum dicoccoides</topic><topic>Triticum dicoccum</topic><topic>Triticum durum</topic><topic>Triticum turgidum subsp. dicoccoides</topic><topic>Triticum turgidum subsp. durum</topic><topic>Vegetals</topic><topic>Wheat</topic><topic>yr15 gene</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sun, G.L</creatorcontrib><creatorcontrib>Fahima, T</creatorcontrib><creatorcontrib>Korol, A.B</creatorcontrib><creatorcontrib>Turpeinen, T</creatorcontrib><creatorcontrib>Grama, A</creatorcontrib><creatorcontrib>Ronin, Y.I</creatorcontrib><creatorcontrib>Nevo, E</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><jtitle>Theoretical and applied genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sun, G.L</au><au>Fahima, T</au><au>Korol, A.B</au><au>Turpeinen, T</au><au>Grama, A</au><au>Ronin, Y.I</au><au>Nevo, E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of molecular markers linked to the Yr15 stripe rust resistance gene of wheat originated in wild emmer wheat, Triticum dicoccoides</atitle><jtitle>Theoretical and applied genetics</jtitle><date>1997-09-01</date><risdate>1997</risdate><volume>95</volume><issue>4</issue><spage>622</spage><epage>628</epage><pages>622-628</pages><issn>0040-5752</issn><eissn>1432-2242</eissn><coden>THAGA6</coden><abstract>The Yr15 gene of wheat confers resistance to the stripe rust pathogen Puccinia striiformis West., which is one of the most devastating diseases of wheat throughout the world. In the present study, molecular markers flanking the Yr15 gene of wheat have been identified using the near-isogenic-lines approach. RFLP screening of 76 probe-enzyme combinations revealed one polymorphic marker (Nor/TaqI) between the susceptible and the resistant lines. In addition, out of 340 RAPD primers tested, six produced polymorphic RAPD bands between the susceptible and the resistant lines. The genetic linkage of the polymorphic markers was tested on segregating F2 population (123 plants) derived from crosses between stripe rust-susceptible Triticum durum wheat, cv D447, and a BC3F9 resistant line carrying Yr15 in a D447 background. A 2.8-kb fragment produced by the Nor RFLP probe and a 1420-bp PCR product generated by the RAPD primer OPB13 showed linkage, in coupling, with the Yr15 gene. Employing the standard maximum- likelihood technique it was found that the order OPB13(1420-) Yr15-Nor1 on chromosome 1B appeared to be no less than 1000-times more probable than the closest alternative. The map distances between OPB13(1420-) Yr15-Nor1 are 27.1 cM and 11.0 cM for the first and second intervals, respectively. The application of marker-assisted selection for the breeding of new wheat cultivars with the stripe rust resistance gene is discussed.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><doi>10.1007/s001220050604</doi><tpages>7</tpages></addata></record> |
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| ispartof | Theoretical and applied genetics, 1997-09, Vol.95 (4), p.622-628 |
| issn | 0040-5752 1432-2242 |
| language | eng |
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| subjects | Biological and medical sciences chromosome mapping Classical genetics, quantitative genetics, hybrids Crop diseases Development and progression disease resistance Diseases and pests Fundamental and applied biological sciences. Psychology genes Genetic aspects genetic markers Genetics Genetics of eukaryotes. Biological and molecular evolution Genomics inheritance (genetics) interspecific hybridization linkage (genetics) Molecular genetics Physiological aspects Plant diseases Plant genetics Plant immunology Pteridophyta, spermatophyta Puccinia striiformis random amplified polymorphic DNA technique restriction fragment length polymorphism rust diseases segregation Triticum aestivum Triticum dicoccoides Triticum dicoccum Triticum durum Triticum turgidum subsp. dicoccoides Triticum turgidum subsp. durum Vegetals Wheat yr15 gene |
| title | Identification of molecular markers linked to the Yr15 stripe rust resistance gene of wheat originated in wild emmer wheat, Triticum dicoccoides |
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