Direct amplification of minisatellite-region DNA with VNTR core sequences in the genus Oryza
A polymerase chain reaction (PCR) application, involving the directed amplification of minisatellite-region DNA (DAMD) with several minisatellite core sequences as primers, was used to detect genetic variation in 17 species of the genus Oryza and several rice cultivars (O. sativa L.). The electropho...
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| Veröffentlicht in: | Theoretical and applied genetics 1997-10, Vol.95 (5/6), p.942-949 |
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| creator | Zhou, Z Bebeli, P.J Somers, D.J Gustafson, J.P |
| description | A polymerase chain reaction (PCR) application, involving the directed amplification of minisatellite-region DNA (DAMD) with several minisatellite core sequences as primers, was used to detect genetic variation in 17 species of the genus Oryza and several rice cultivars (O. sativa L.). The electrophoretic analysis of DAMD-PCR products showed high levels of variation between different species and little variation between different cultivars of O. sativa. Polymorphisms were also found between accessions within a species, and between individual plants within an accession of several wild species. The DAMD-PCR yielded genome-specific banding patterns for the species studied. Several DAMD-PCR-generated DNA fragments were cloned and characterized. One clone was capable of detecting multiple fragments and revealed individual-specific hybridization banding patterns using genomic DNA from wild species as well as rice cultivars. A second clone detected only a single polymorphic locus, while a third clone expressed a strong genome specificity by Southern analysis. The results demonstrated that DAMD-PCR is potentially useful for species and genome identification in Oryza. The DAMD-PCR technique also allows for the isolation of informative molecular probes to be utilized in DNA fingerprinting and genome identification in rice. |
| doi_str_mv | 10.1007/s001220050645 |
| format | Article |
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The electrophoretic analysis of DAMD-PCR products showed high levels of variation between different species and little variation between different cultivars of O. sativa. Polymorphisms were also found between accessions within a species, and between individual plants within an accession of several wild species. The DAMD-PCR yielded genome-specific banding patterns for the species studied. Several DAMD-PCR-generated DNA fragments were cloned and characterized. One clone was capable of detecting multiple fragments and revealed individual-specific hybridization banding patterns using genomic DNA from wild species as well as rice cultivars. A second clone detected only a single polymorphic locus, while a third clone expressed a strong genome specificity by Southern analysis. The results demonstrated that DAMD-PCR is potentially useful for species and genome identification in Oryza. The DAMD-PCR technique also allows for the isolation of informative molecular probes to be utilized in DNA fingerprinting and genome identification in rice.</description><identifier>ISSN: 0040-5752</identifier><identifier>EISSN: 1432-2242</identifier><identifier>DOI: 10.1007/s001220050645</identifier><identifier>CODEN: THAGA6</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>alleles ; Biological and medical sciences ; Classical genetics, quantitative genetics, hybrids ; Cultivars ; Deoxyribonucleic acid ; DNA ; DNA fingerprinting ; Fundamental and applied biological sciences. Psychology ; Gene amplification ; Genetic aspects ; genetic polymorphism ; Genetic variation ; Genetics of eukaryotes. Biological and molecular evolution ; Genomes ; Identification and classification ; loci ; microsatellite repeats ; minisatellite repeats ; molecular sequence data ; nucleotide sequences ; Oryza ; Oryza sativa ; Plant genetics ; polymerase chain reaction ; Pteridophyta, spermatophyta ; repetitive sequences ; Rice ; Southern blotting ; Vegetals ; wild relatives</subject><ispartof>Theoretical and applied genetics, 1997-10, Vol.95 (5/6), p.942-949</ispartof><rights>1998 INIST-CNRS</rights><rights>COPYRIGHT 1997 Springer</rights><rights>Springer-Verlag Berlin Heidelberg 1997</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c440t-acc30e22cab5d14b57ea7c4fc411917b48fd0595b5e8b17bb4e1f6de3d7f53593</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2048999$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhou, Z</creatorcontrib><creatorcontrib>Bebeli, P.J</creatorcontrib><creatorcontrib>Somers, D.J</creatorcontrib><creatorcontrib>Gustafson, J.P</creatorcontrib><title>Direct amplification of minisatellite-region DNA with VNTR core sequences in the genus Oryza</title><title>Theoretical and applied genetics</title><description>A polymerase chain reaction (PCR) application, involving the directed amplification of minisatellite-region DNA (DAMD) with several minisatellite core sequences as primers, was used to detect genetic variation in 17 species of the genus Oryza and several rice cultivars (O. sativa L.). The electrophoretic analysis of DAMD-PCR products showed high levels of variation between different species and little variation between different cultivars of O. sativa. Polymorphisms were also found between accessions within a species, and between individual plants within an accession of several wild species. The DAMD-PCR yielded genome-specific banding patterns for the species studied. Several DAMD-PCR-generated DNA fragments were cloned and characterized. One clone was capable of detecting multiple fragments and revealed individual-specific hybridization banding patterns using genomic DNA from wild species as well as rice cultivars. A second clone detected only a single polymorphic locus, while a third clone expressed a strong genome specificity by Southern analysis. The results demonstrated that DAMD-PCR is potentially useful for species and genome identification in Oryza. The DAMD-PCR technique also allows for the isolation of informative molecular probes to be utilized in DNA fingerprinting and genome identification in rice.</description><subject>alleles</subject><subject>Biological and medical sciences</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>Cultivars</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA fingerprinting</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene amplification</subject><subject>Genetic aspects</subject><subject>genetic polymorphism</subject><subject>Genetic variation</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Genomes</subject><subject>Identification and classification</subject><subject>loci</subject><subject>microsatellite repeats</subject><subject>minisatellite repeats</subject><subject>molecular sequence data</subject><subject>nucleotide sequences</subject><subject>Oryza</subject><subject>Oryza sativa</subject><subject>Plant genetics</subject><subject>polymerase chain reaction</subject><subject>Pteridophyta, spermatophyta</subject><subject>repetitive sequences</subject><subject>Rice</subject><subject>Southern blotting</subject><subject>Vegetals</subject><subject>wild relatives</subject><issn>0040-5752</issn><issn>1432-2242</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp90cuLFDEQB-BGFBxXj54NKoqH1sqrH8dhH7qw7MI-PAkhnanMZulJZpM0uv71pplFGA-eQsKXyq9SVfWawmcK0H5JAJQxAAmNkE-qBRWc1YwJ9rRaAAioZSvZ8-pFSncAwCTwRfXjyEU0mejNdnTWGZ1d8CRYsnHeJZ1xHF3GOuJ6Pj86X5KfLt-S7-fXl8SEiCTh_YTeYCLOk3yLZI1-SuQiPvzWL6tnVo8JXz2uB9XNyfH14bf67OLr6eHyrDZCQK61MRyQMaMHuaJikC3q1ghrBKU9bQfR2RXIXg4Su6HsB4HUNivkq9ZKLnt-UH3c1d3GUNKkrDYumRJdewxTUn3T0bYXsi3yw38lbThvaDvDt__AuzBFX7pQnewppaJpCnq3Q2s9onLehhy1mSuqJQfoy5udKOrTnjLBZ_yV13pKSZ1eXe7bemdNDClFtGob3UbHB0VBzUNWe0Mu_v1jTJ2MHm3U3rj09xID0fX9_EFvdszqoPQ6FnJzxYByYF0HQnD-B1airGk</recordid><startdate>19971001</startdate><enddate>19971001</enddate><creator>Zhou, Z</creator><creator>Bebeli, P.J</creator><creator>Somers, D.J</creator><creator>Gustafson, J.P</creator><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7SS</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7TM</scope></search><sort><creationdate>19971001</creationdate><title>Direct amplification of minisatellite-region DNA with VNTR core sequences in the genus Oryza</title><author>Zhou, Z ; Bebeli, P.J ; Somers, D.J ; Gustafson, J.P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c440t-acc30e22cab5d14b57ea7c4fc411917b48fd0595b5e8b17bb4e1f6de3d7f53593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>alleles</topic><topic>Biological and medical sciences</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>Cultivars</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA fingerprinting</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene amplification</topic><topic>Genetic aspects</topic><topic>genetic polymorphism</topic><topic>Genetic variation</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Genomes</topic><topic>Identification and classification</topic><topic>loci</topic><topic>microsatellite repeats</topic><topic>minisatellite repeats</topic><topic>molecular sequence data</topic><topic>nucleotide sequences</topic><topic>Oryza</topic><topic>Oryza sativa</topic><topic>Plant genetics</topic><topic>polymerase chain reaction</topic><topic>Pteridophyta, spermatophyta</topic><topic>repetitive sequences</topic><topic>Rice</topic><topic>Southern blotting</topic><topic>Vegetals</topic><topic>wild relatives</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhou, Z</creatorcontrib><creatorcontrib>Bebeli, P.J</creatorcontrib><creatorcontrib>Somers, D.J</creatorcontrib><creatorcontrib>Gustafson, J.P</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Theoretical and applied genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhou, Z</au><au>Bebeli, P.J</au><au>Somers, D.J</au><au>Gustafson, J.P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Direct amplification of minisatellite-region DNA with VNTR core sequences in the genus Oryza</atitle><jtitle>Theoretical and applied genetics</jtitle><date>1997-10-01</date><risdate>1997</risdate><volume>95</volume><issue>5/6</issue><spage>942</spage><epage>949</epage><pages>942-949</pages><issn>0040-5752</issn><eissn>1432-2242</eissn><coden>THAGA6</coden><abstract>A polymerase chain reaction (PCR) application, involving the directed amplification of minisatellite-region DNA (DAMD) with several minisatellite core sequences as primers, was used to detect genetic variation in 17 species of the genus Oryza and several rice cultivars (O. sativa L.). The electrophoretic analysis of DAMD-PCR products showed high levels of variation between different species and little variation between different cultivars of O. sativa. Polymorphisms were also found between accessions within a species, and between individual plants within an accession of several wild species. The DAMD-PCR yielded genome-specific banding patterns for the species studied. Several DAMD-PCR-generated DNA fragments were cloned and characterized. One clone was capable of detecting multiple fragments and revealed individual-specific hybridization banding patterns using genomic DNA from wild species as well as rice cultivars. A second clone detected only a single polymorphic locus, while a third clone expressed a strong genome specificity by Southern analysis. The results demonstrated that DAMD-PCR is potentially useful for species and genome identification in Oryza. The DAMD-PCR technique also allows for the isolation of informative molecular probes to be utilized in DNA fingerprinting and genome identification in rice.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><doi>10.1007/s001220050645</doi><tpages>8</tpages></addata></record> |
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| ispartof | Theoretical and applied genetics, 1997-10, Vol.95 (5/6), p.942-949 |
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| subjects | alleles Biological and medical sciences Classical genetics, quantitative genetics, hybrids Cultivars Deoxyribonucleic acid DNA DNA fingerprinting Fundamental and applied biological sciences. Psychology Gene amplification Genetic aspects genetic polymorphism Genetic variation Genetics of eukaryotes. Biological and molecular evolution Genomes Identification and classification loci microsatellite repeats minisatellite repeats molecular sequence data nucleotide sequences Oryza Oryza sativa Plant genetics polymerase chain reaction Pteridophyta, spermatophyta repetitive sequences Rice Southern blotting Vegetals wild relatives |
| title | Direct amplification of minisatellite-region DNA with VNTR core sequences in the genus Oryza |
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