bacterial artificial chromosome library for soybean and identification of clones near a major cyst nematode resistance gene
We constructed a bacterial artificial chromosome (BAC) library for soybean (Glycine max) consisting of approximately 30000 clones with an average insert size of 120 kilobase pairs. The library was successfully screened with restriction fragment length polymorphism (RFLP) and microsatellite markers t...
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| Veröffentlicht in: | Theoretical and applied genetics 1998-02, Vol.96 (2), p.196-202 |
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| container_title | Theoretical and applied genetics |
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| creator | Danesh, D Penuela, S Mudge, J Denny, R.L Nordstrom, H Martinez, J.P Young, N.D |
| description | We constructed a bacterial artificial chromosome (BAC) library for soybean (Glycine max) consisting of approximately 30000 clones with an average insert size of 120 kilobase pairs. The library was successfully screened with restriction fragment length polymorphism (RFLP) and microsatellite markers tightly linked to a major resistance gene for the cyst nematode, Heterodera glycines. Since many soybean RFLPs hybridize to duplicate loci, BACs homologous to duplicate RFLP loci were distinguished by digestion with the restriction enzyme originally used to map the RFLP, followed by a comparison of the hybridizing fragments. Linkage mapping of BAC clones identified with markers linked to the cyst nematode resistance gene demonstrated that these clones were located at the expected chromosomal positions and that there were no indications of chimeras within the genomic inserts. |
| doi_str_mv | 10.1007/s001220050727 |
| format | Article |
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The library was successfully screened with restriction fragment length polymorphism (RFLP) and microsatellite markers tightly linked to a major resistance gene for the cyst nematode, Heterodera glycines. Since many soybean RFLPs hybridize to duplicate loci, BACs homologous to duplicate RFLP loci were distinguished by digestion with the restriction enzyme originally used to map the RFLP, followed by a comparison of the hybridizing fragments. Linkage mapping of BAC clones identified with markers linked to the cyst nematode resistance gene demonstrated that these clones were located at the expected chromosomal positions and that there were no indications of chimeras within the genomic inserts.</description><identifier>ISSN: 0040-5752</identifier><identifier>EISSN: 1432-2242</identifier><identifier>DOI: 10.1007/s001220050727</identifier><identifier>CODEN: THAGA6</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Biological and medical sciences ; chromosome mapping ; Classical genetics, quantitative genetics, hybrids ; clones ; cloning ; Cysts ; DNA libraries ; Fundamental and applied biological sciences. Psychology ; Genetic aspects ; genetic markers ; Genetics ; Genetics of eukaryotes. Biological and molecular evolution ; Genomic libraries ; Glycine max ; Heterodera glycines ; Libraries ; linkage (genetics) ; major genes ; microsatellite repeats ; Nematoda ; pest resistance ; Plant genetics ; Plant immunology ; Pteridophyta, spermatophyta ; repetitive sequences ; restriction fragment length polymorphism ; Soybean ; Soybeans ; Vegetals</subject><ispartof>Theoretical and applied genetics, 1998-02, Vol.96 (2), p.196-202</ispartof><rights>1998 INIST-CNRS</rights><rights>COPYRIGHT 1998 Springer</rights><rights>Springer-Verlag Berlin Heidelberg 1998</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c478t-ff8df7a4f998926e35199a182a53e33140f9e4f5487cdd44f25c6ff16a02993f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2156052$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Danesh, D</creatorcontrib><creatorcontrib>Penuela, S</creatorcontrib><creatorcontrib>Mudge, J</creatorcontrib><creatorcontrib>Denny, R.L</creatorcontrib><creatorcontrib>Nordstrom, H</creatorcontrib><creatorcontrib>Martinez, J.P</creatorcontrib><creatorcontrib>Young, N.D</creatorcontrib><title>bacterial artificial chromosome library for soybean and identification of clones near a major cyst nematode resistance gene</title><title>Theoretical and applied genetics</title><description>We constructed a bacterial artificial chromosome (BAC) library for soybean (Glycine max) consisting of approximately 30000 clones with an average insert size of 120 kilobase pairs. The library was successfully screened with restriction fragment length polymorphism (RFLP) and microsatellite markers tightly linked to a major resistance gene for the cyst nematode, Heterodera glycines. Since many soybean RFLPs hybridize to duplicate loci, BACs homologous to duplicate RFLP loci were distinguished by digestion with the restriction enzyme originally used to map the RFLP, followed by a comparison of the hybridizing fragments. Linkage mapping of BAC clones identified with markers linked to the cyst nematode resistance gene demonstrated that these clones were located at the expected chromosomal positions and that there were no indications of chimeras within the genomic inserts.</description><subject>Biological and medical sciences</subject><subject>chromosome mapping</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>clones</subject><subject>cloning</subject><subject>Cysts</subject><subject>DNA libraries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic aspects</subject><subject>genetic markers</subject><subject>Genetics</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Genomic libraries</subject><subject>Glycine max</subject><subject>Heterodera glycines</subject><subject>Libraries</subject><subject>linkage (genetics)</subject><subject>major genes</subject><subject>microsatellite repeats</subject><subject>Nematoda</subject><subject>pest resistance</subject><subject>Plant genetics</subject><subject>Plant immunology</subject><subject>Pteridophyta, spermatophyta</subject><subject>repetitive sequences</subject><subject>restriction fragment length polymorphism</subject><subject>Soybean</subject><subject>Soybeans</subject><subject>Vegetals</subject><issn>0040-5752</issn><issn>1432-2242</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp90c1rFDEUAPBBFFxbj54NIoqHqS-fMzmW4kehILT2HN5mXtYsM0lNZqGL_7yzbhH00FMe4fce76NpXnE44wDdxwrAhQDQ0InuSbPiSopWCCWeNisABa3utHjevKh1CwBCg1w1v9boZyoRR4ZljiH6Q-h_lDzlmidiY1wXLHsWcmE179eEiWEaWBwo_fE4x5xYDsyPOVFlibAwZBNulwy_r_PyM-GcB2KFaqwzJk9sQ4lOm2cBx0ovH96T5vbzp-8XX9urb18uL86vWq-6fm5D6IfQoQrW9lYYkppbi7wXqCVJyRUESypo1Xd-GJQKQnsTAjcIwloZ5Enz_lj3ruSfO6qzm2L1NI6YKO-qs6bnnZWCL_Ldo5IbabQ0B_jmP7jNu5KWKVyvLeeCG7GgD0e0wZFcTD6nme7nDe5qdZc31-5cAjfKqF4utj1aX3KthYK7K3FaFu84uMN13T_XXfzbhwawehxDWbYa698kwbUBfWjh9ZEFzA43ZSG3NwK4BNH3hutO_gZJz6y8</recordid><startdate>19980201</startdate><enddate>19980201</enddate><creator>Danesh, D</creator><creator>Penuela, S</creator><creator>Mudge, J</creator><creator>Denny, R.L</creator><creator>Nordstrom, H</creator><creator>Martinez, J.P</creator><creator>Young, N.D</creator><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7SS</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>19980201</creationdate><title>bacterial artificial chromosome library for soybean and identification of clones near a major cyst nematode resistance gene</title><author>Danesh, D ; Penuela, S ; Mudge, J ; Denny, R.L ; Nordstrom, H ; Martinez, J.P ; Young, N.D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c478t-ff8df7a4f998926e35199a182a53e33140f9e4f5487cdd44f25c6ff16a02993f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Biological and medical sciences</topic><topic>chromosome mapping</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>clones</topic><topic>cloning</topic><topic>Cysts</topic><topic>DNA libraries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic aspects</topic><topic>genetic markers</topic><topic>Genetics</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Genomic libraries</topic><topic>Glycine max</topic><topic>Heterodera glycines</topic><topic>Libraries</topic><topic>linkage (genetics)</topic><topic>major genes</topic><topic>microsatellite repeats</topic><topic>Nematoda</topic><topic>pest resistance</topic><topic>Plant genetics</topic><topic>Plant immunology</topic><topic>Pteridophyta, spermatophyta</topic><topic>repetitive sequences</topic><topic>restriction fragment length polymorphism</topic><topic>Soybean</topic><topic>Soybeans</topic><topic>Vegetals</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Danesh, D</creatorcontrib><creatorcontrib>Penuela, S</creatorcontrib><creatorcontrib>Mudge, J</creatorcontrib><creatorcontrib>Denny, R.L</creatorcontrib><creatorcontrib>Nordstrom, H</creatorcontrib><creatorcontrib>Martinez, J.P</creatorcontrib><creatorcontrib>Young, N.D</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Health Medical collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest Biological Science Journals</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Theoretical and applied genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Danesh, D</au><au>Penuela, S</au><au>Mudge, J</au><au>Denny, R.L</au><au>Nordstrom, H</au><au>Martinez, J.P</au><au>Young, N.D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>bacterial artificial chromosome library for soybean and identification of clones near a major cyst nematode resistance gene</atitle><jtitle>Theoretical and applied genetics</jtitle><date>1998-02-01</date><risdate>1998</risdate><volume>96</volume><issue>2</issue><spage>196</spage><epage>202</epage><pages>196-202</pages><issn>0040-5752</issn><eissn>1432-2242</eissn><coden>THAGA6</coden><abstract>We constructed a bacterial artificial chromosome (BAC) library for soybean (Glycine max) consisting of approximately 30000 clones with an average insert size of 120 kilobase pairs. The library was successfully screened with restriction fragment length polymorphism (RFLP) and microsatellite markers tightly linked to a major resistance gene for the cyst nematode, Heterodera glycines. Since many soybean RFLPs hybridize to duplicate loci, BACs homologous to duplicate RFLP loci were distinguished by digestion with the restriction enzyme originally used to map the RFLP, followed by a comparison of the hybridizing fragments. Linkage mapping of BAC clones identified with markers linked to the cyst nematode resistance gene demonstrated that these clones were located at the expected chromosomal positions and that there were no indications of chimeras within the genomic inserts.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><doi>10.1007/s001220050727</doi><tpages>7</tpages></addata></record> |
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| ispartof | Theoretical and applied genetics, 1998-02, Vol.96 (2), p.196-202 |
| issn | 0040-5752 1432-2242 |
| language | eng |
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| source | SpringerNature Journals |
| subjects | Biological and medical sciences chromosome mapping Classical genetics, quantitative genetics, hybrids clones cloning Cysts DNA libraries Fundamental and applied biological sciences. Psychology Genetic aspects genetic markers Genetics Genetics of eukaryotes. Biological and molecular evolution Genomic libraries Glycine max Heterodera glycines Libraries linkage (genetics) major genes microsatellite repeats Nematoda pest resistance Plant genetics Plant immunology Pteridophyta, spermatophyta repetitive sequences restriction fragment length polymorphism Soybean Soybeans Vegetals |
| title | bacterial artificial chromosome library for soybean and identification of clones near a major cyst nematode resistance gene |
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