Saturation mapping of a major gene for resistance to white pine blister rust in sugar pine
The molecular basis of resistance to diseases in plants can be better understood if the genes coding for resistance can be cloned. The single major dominant gene (R) that confers resistance to the white pine blister rust fungus (Cronartium ribicola Fisch.) in sugar pine (Pinus lambertiana Dougl.) ha...
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| Veröffentlicht in: | Theoretical and applied genetics 1998-12, Vol.97 (8), p.1355-1360 |
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| container_title | Theoretical and applied genetics |
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| creator | Harkins, D.M Johnson, G.N Skaggs, P.A Mix, A.D Dupper, G.E Devey, M.E Klinlock, B.B. Jr Neale, D.B |
| description | The molecular basis of resistance to diseases in plants can be better understood if the genes coding for resistance can be cloned. The single major dominant gene (R) that confers resistance to the white pine blister rust fungus (Cronartium ribicola Fisch.) in sugar pine (Pinus lambertiana Dougl.) has been previously mapped. The objectives of the present study were to saturate the region flanking R with tightly linked markers and to construct genetic maps for each of four individual seed trees. Bulked segregant analysis (BSA) and haploid segregation analysis were employed to identify random amplified polymorphic DNA (RAPD) markers linked to R. Automated PCR analysis was used to assay 1115 primers with susceptible and resistant DNA pools from each of four seed trees (8920 PCR reactions). Thirteen RAPD loci were identified that were linked to R. The linkage analyses programs Join-Map 1.4 and Mapmaker 2.0 were used to order RAPD loci relative to R and to construct maps for each of the individual seed trees. Two seed trees, 5701 and 6000, had a large number of tightly linked markers flanking R. These trees will be used in subsequent high-resolution mapping experiments to identify very tightly linked markers to facilitate the eventual cloning of R. |
| doi_str_mv | 10.1007/s001220051029 |
| format | Article |
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Jr ; Neale, D.B</creator><creatorcontrib>Harkins, D.M ; Johnson, G.N ; Skaggs, P.A ; Mix, A.D ; Dupper, G.E ; Devey, M.E ; Klinlock, B.B. Jr ; Neale, D.B ; G. N . Johnson</creatorcontrib><description>The molecular basis of resistance to diseases in plants can be better understood if the genes coding for resistance can be cloned. The single major dominant gene (R) that confers resistance to the white pine blister rust fungus (Cronartium ribicola Fisch.) in sugar pine (Pinus lambertiana Dougl.) has been previously mapped. The objectives of the present study were to saturate the region flanking R with tightly linked markers and to construct genetic maps for each of four individual seed trees. Bulked segregant analysis (BSA) and haploid segregation analysis were employed to identify random amplified polymorphic DNA (RAPD) markers linked to R. Automated PCR analysis was used to assay 1115 primers with susceptible and resistant DNA pools from each of four seed trees (8920 PCR reactions). Thirteen RAPD loci were identified that were linked to R. The linkage analyses programs Join-Map 1.4 and Mapmaker 2.0 were used to order RAPD loci relative to R and to construct maps for each of the individual seed trees. Two seed trees, 5701 and 6000, had a large number of tightly linked markers flanking R. These trees will be used in subsequent high-resolution mapping experiments to identify very tightly linked markers to facilitate the eventual cloning of R.</description><identifier>ISSN: 0040-5752</identifier><identifier>EISSN: 1432-2242</identifier><identifier>DOI: 10.1007/s001220051029</identifier><identifier>CODEN: THAGA6</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Biological and medical sciences ; bulked segregant analysis ; chromosome mapping ; Classical genetics, quantitative genetics, hybrids ; Cronartium ribicola ; Development and progression ; disease resistance ; Diseases and pests ; Fundamental and applied biological sciences. Psychology ; Genetic aspects ; Genetic markers ; genetic resistance ; genetic techniques and protocols ; Genetics ; Genetics of eukaryotes. Biological and molecular evolution ; haploid segregation analysis ; linkage groups ; loci ; major genes ; Physiological aspects ; Pine ; Pinus lambertiana ; Plant genetics ; Plant immunology ; Pteridophyta, spermatophyta ; random amplified polymorphic DNA technique ; Rust diseases ; seed trees ; segregation ; Vegetals</subject><ispartof>Theoretical and applied genetics, 1998-12, Vol.97 (8), p.1355-1360</ispartof><rights>1999 INIST-CNRS</rights><rights>COPYRIGHT 1998 Springer</rights><rights>Springer-Verlag Berlin Heidelberg 1998</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c447t-3a027521dd563adf5fbd456847d808522ab0e9438c061887e505989bb12a94843</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1626600$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Harkins, D.M</creatorcontrib><creatorcontrib>Johnson, G.N</creatorcontrib><creatorcontrib>Skaggs, P.A</creatorcontrib><creatorcontrib>Mix, A.D</creatorcontrib><creatorcontrib>Dupper, G.E</creatorcontrib><creatorcontrib>Devey, M.E</creatorcontrib><creatorcontrib>Klinlock, B.B. Jr</creatorcontrib><creatorcontrib>Neale, D.B</creatorcontrib><creatorcontrib>G. N . Johnson</creatorcontrib><title>Saturation mapping of a major gene for resistance to white pine blister rust in sugar pine</title><title>Theoretical and applied genetics</title><description>The molecular basis of resistance to diseases in plants can be better understood if the genes coding for resistance can be cloned. The single major dominant gene (R) that confers resistance to the white pine blister rust fungus (Cronartium ribicola Fisch.) in sugar pine (Pinus lambertiana Dougl.) has been previously mapped. The objectives of the present study were to saturate the region flanking R with tightly linked markers and to construct genetic maps for each of four individual seed trees. Bulked segregant analysis (BSA) and haploid segregation analysis were employed to identify random amplified polymorphic DNA (RAPD) markers linked to R. Automated PCR analysis was used to assay 1115 primers with susceptible and resistant DNA pools from each of four seed trees (8920 PCR reactions). Thirteen RAPD loci were identified that were linked to R. The linkage analyses programs Join-Map 1.4 and Mapmaker 2.0 were used to order RAPD loci relative to R and to construct maps for each of the individual seed trees. Two seed trees, 5701 and 6000, had a large number of tightly linked markers flanking R. These trees will be used in subsequent high-resolution mapping experiments to identify very tightly linked markers to facilitate the eventual cloning of R.</description><subject>Biological and medical sciences</subject><subject>bulked segregant analysis</subject><subject>chromosome mapping</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>Cronartium ribicola</subject><subject>Development and progression</subject><subject>disease resistance</subject><subject>Diseases and pests</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic aspects</subject><subject>Genetic markers</subject><subject>genetic resistance</subject><subject>genetic techniques and protocols</subject><subject>Genetics</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>haploid segregation analysis</subject><subject>linkage groups</subject><subject>loci</subject><subject>major genes</subject><subject>Physiological aspects</subject><subject>Pine</subject><subject>Pinus lambertiana</subject><subject>Plant genetics</subject><subject>Plant immunology</subject><subject>Pteridophyta, spermatophyta</subject><subject>random amplified polymorphic DNA technique</subject><subject>Rust diseases</subject><subject>seed trees</subject><subject>segregation</subject><subject>Vegetals</subject><issn>0040-5752</issn><issn>1432-2242</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpdkc9rFTEQx4Mo-KwePRtEkB62Tn5tssdS1BYKBZ-9eAmzu9k1j33JM8nS-t-b9hVEL_Mj85nJlxlC3jI4YwD6UwZgnAMoBrx7RjZMCt5wLvlzsgGQ0Cit-EvyKucdAHAFYkN-bLGsCYuPge7xcPBhpnGiWJNdTHR2wdGpBsllnwuGwdES6d1PXxytsKP9Ut9dBdZcqA80rzOmx9Jr8mLCJbs3T_6E3H75_P3isrm--Xp1cX7dDFLq0ggEXnWxcVStwHFSUz9K1RqpRwNGcY49uE4KM0DLjNFOgepM1_eMYyeNFCfk43HuIcVfq8vF7n0e3LJgcHHNtmsN06bupZLv_yN3cU2hirNGdYxpYF2FTo_QjIuzPgwxFHdfZlxztlfbb_ZcABOmmoeBzZEdUsw5uckekt9j-m0Z2Ieb2H9uUvkPTwIwD7hMqS7U579NLW_bR53vjtiE0eKcKnK75fVX4KbTRnPxBxJQkU4</recordid><startdate>19981201</startdate><enddate>19981201</enddate><creator>Harkins, D.M</creator><creator>Johnson, G.N</creator><creator>Skaggs, P.A</creator><creator>Mix, A.D</creator><creator>Dupper, G.E</creator><creator>Devey, M.E</creator><creator>Klinlock, B.B. 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Jr ; Neale, D.B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c447t-3a027521dd563adf5fbd456847d808522ab0e9438c061887e505989bb12a94843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Biological and medical sciences</topic><topic>bulked segregant analysis</topic><topic>chromosome mapping</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>Cronartium ribicola</topic><topic>Development and progression</topic><topic>disease resistance</topic><topic>Diseases and pests</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic aspects</topic><topic>Genetic markers</topic><topic>genetic resistance</topic><topic>genetic techniques and protocols</topic><topic>Genetics</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>haploid segregation analysis</topic><topic>linkage groups</topic><topic>loci</topic><topic>major genes</topic><topic>Physiological aspects</topic><topic>Pine</topic><topic>Pinus lambertiana</topic><topic>Plant genetics</topic><topic>Plant immunology</topic><topic>Pteridophyta, spermatophyta</topic><topic>random amplified polymorphic DNA technique</topic><topic>Rust diseases</topic><topic>seed trees</topic><topic>segregation</topic><topic>Vegetals</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Harkins, D.M</creatorcontrib><creatorcontrib>Johnson, G.N</creatorcontrib><creatorcontrib>Skaggs, P.A</creatorcontrib><creatorcontrib>Mix, A.D</creatorcontrib><creatorcontrib>Dupper, G.E</creatorcontrib><creatorcontrib>Devey, M.E</creatorcontrib><creatorcontrib>Klinlock, B.B. Jr</creatorcontrib><creatorcontrib>Neale, D.B</creatorcontrib><creatorcontrib>G. N . 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Jr</au><au>Neale, D.B</au><aucorp>G. N . Johnson</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Saturation mapping of a major gene for resistance to white pine blister rust in sugar pine</atitle><jtitle>Theoretical and applied genetics</jtitle><date>1998-12-01</date><risdate>1998</risdate><volume>97</volume><issue>8</issue><spage>1355</spage><epage>1360</epage><pages>1355-1360</pages><issn>0040-5752</issn><eissn>1432-2242</eissn><coden>THAGA6</coden><abstract>The molecular basis of resistance to diseases in plants can be better understood if the genes coding for resistance can be cloned. The single major dominant gene (R) that confers resistance to the white pine blister rust fungus (Cronartium ribicola Fisch.) in sugar pine (Pinus lambertiana Dougl.) has been previously mapped. The objectives of the present study were to saturate the region flanking R with tightly linked markers and to construct genetic maps for each of four individual seed trees. Bulked segregant analysis (BSA) and haploid segregation analysis were employed to identify random amplified polymorphic DNA (RAPD) markers linked to R. Automated PCR analysis was used to assay 1115 primers with susceptible and resistant DNA pools from each of four seed trees (8920 PCR reactions). Thirteen RAPD loci were identified that were linked to R. The linkage analyses programs Join-Map 1.4 and Mapmaker 2.0 were used to order RAPD loci relative to R and to construct maps for each of the individual seed trees. Two seed trees, 5701 and 6000, had a large number of tightly linked markers flanking R. These trees will be used in subsequent high-resolution mapping experiments to identify very tightly linked markers to facilitate the eventual cloning of R.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><doi>10.1007/s001220051029</doi><tpages>6</tpages></addata></record> |
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| subjects | Biological and medical sciences bulked segregant analysis chromosome mapping Classical genetics, quantitative genetics, hybrids Cronartium ribicola Development and progression disease resistance Diseases and pests Fundamental and applied biological sciences. Psychology Genetic aspects Genetic markers genetic resistance genetic techniques and protocols Genetics Genetics of eukaryotes. Biological and molecular evolution haploid segregation analysis linkage groups loci major genes Physiological aspects Pine Pinus lambertiana Plant genetics Plant immunology Pteridophyta, spermatophyta random amplified polymorphic DNA technique Rust diseases seed trees segregation Vegetals |
| title | Saturation mapping of a major gene for resistance to white pine blister rust in sugar pine |
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