Karyotyping of human chondrocytes in scaffold-assisted cartilage tissue engineering

Scaffold-assisted autologous chondrocyte implantation (ACI) is an effective clinical procedure for cartilage repair. The aim of our study was to evaluate the chromosomal stability of human chondrocytes subjected to typical cell culture procedures needed for regenerative approaches in polymer-scaffol...

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Veröffentlicht in:	Acta biomaterialia 2012-04, Vol.8 (4), p.1519-1529
Hauptverfasser:	Trimborn, Marc, Endres, Michaela, Bommer, Christiane, Janke, Una, Krüger, Jan-Philipp, Morawietz, Lars, Kreuz, Peter C., Kaps, Christian
Format:	Artikel
Sprache:	eng
Schlagworte:	ACI Adult Biomarkers - metabolism Cartilage Cartilage regeneration Cartilage tissue engineering Cartilage, Articular - cytology Cartilage, Articular - drug effects Cartilage, Articular - physiology cell culture Cell Differentiation - drug effects Cell Survival - drug effects Chondrocyte differentiation chondrocytes Chondrocytes - cytology Chondrocytes - drug effects Chondrocytes - metabolism chromosome aberrations Chromosome Aberrations - drug effects collagen cryopreservation Culture electron microscopy extracellular matrix Female Fibrin - pharmacology Fluoresceins - metabolism fluorescence in situ hybridization gene expression Gene Expression Regulation - drug effects genetic markers genome Genomes histology Human Humans Karyotype karyotyping Karyotyping - methods Male medicine Microscopy, Electron, Scanning Middle Aged Polyglycolic Acid - pharmacology Postmortem Changes Propidium - metabolism Real-Time Polymerase Chain Reaction Regenerative Repair Resorbable polymer scaffold Scaffolds tissue culture Tissue Donors tissue engineering Tissue Engineering - methods tissue scaffolds Tissue Scaffolds - chemistry Transplantation, Autologous Young Adult
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