Identification of immunogenic proteins of Flavobacterium columnare by two‐dimensional electrophoresis immunoblotting with antibacterial sera from grass carp, Ctenopharyngodon idella (Valenciennes)

Flavobacterium columnare is a Gram‐negative bacterium causing columnaris disease of freshwater fish worldwide, and development of efficacious vaccines has been a continuous challenge in aquaculture. In this study, 14 proteins were identified from cellular components of F. columnare using an immunobl...

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Veröffentlicht in:	Journal of fish diseases 2012-04, Vol.35 (4), p.255-263
Hauptverfasser:	Liu, Z X, Liu, G Y, Li, N, Xiao, F S, Xie, H X, Nie, P
Format:	Artikel
Sprache:	eng
Schlagworte:	alcohol dehydrogenase Amino Acid Sequence Animals Aquaculture bacteria bacterial antigens bacterial infections bacterial proteins Bacterial Proteins - immunology Bacterial Proteins - isolation & purification Carps - immunology chaperonins Ctenopharyngodon idella Electrophoresis, Gel, Two-Dimensional fish culture fish diseases Fish Diseases - immunology Fish Diseases - microbiology Flavobacteriaceae Infections - immunology Flavobacteriaceae Infections - microbiology Flavobacteriaceae Infections - veterinary Flavobacterium - immunology Flavobacterium columnare Freshwater fructose-bisphosphate aldolase grass carp Immune Sera Immunoblotting immunogenic protein lasers Mass Spectrometry Molecular Sequence Data polyclonal antibodies regulatory proteins ribosomal proteins Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization spectroscopy two-dimensional gel electrophoresis vaccine development
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