Regulation of Carotenoid Content in Tomato by Silencing of Lycopene β/ε-Cyclase Genes

To conduct RNAi interference of Lyc-β and Lyc-ε genes, two plant expression vectors were constructed by inserting the intron fragments of the gusA gene into the two target gene fragments, which were designed in anti-sense directions. After the Agrobacterium tumefaciens-mediated transformation, 13 tr...

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Veröffentlicht in:	Plant molecular biology reporter 2011-03, Vol.29 (1), p.117-124
Hauptverfasser:	Ma, Chao, Ma, Binggang, He, Juan, Hao, Qingnan, Lu, Xiaoyan, Wang, Lei
Format:	Artikel
Sprache:	eng
Schlagworte:	Abundance Agrobacterium Antisense RNA Bioinformatics Biomedical and Life Sciences Carotenoids Expression vectors Fragments Gene expression Genes Genetic transformation GusA gene High performance liquid chromatography Interference Leaves Life Sciences Liquid chromatography Lutein Lycopene Metabolomics mRNA Plant Breeding/Biotechnology Plant Sciences Proteomics Ribonucleic acid RNA RNA-mediated interference Tomatoes Transgenic plants β-Carotene
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