Phylogenetic Analysis of Methanogens in the Pig Feces

In order to assess methanogen diversity in feces of pigs, archaeal 16S rRNA gene clone libraries were constructed from feces of the pig. After the amplification by PCR using primers Met86F and Met1340R, equal quantities of PCR products from each of the five pigs were mixed together and used to const...

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Veröffentlicht in:	Current microbiology 2011-05, Vol.62 (5), p.1386-1389
Hauptverfasser:	Mao, Sheng-Yong, Yang, Cui-Feng, Zhu, Wei-Yun
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Archaea Archaea - classification Archaea - genetics Archaea - isolation & purification Archaea - metabolism Bacteriology Biomedical and Life Sciences Biotechnology clones digestive system DNA, Archaeal - genetics Euryarchaeota Feces Feces - microbiology genes Genetics Hogs Life Sciences Methane - metabolism Methanobrevibacter methanogens Microbiology Molecular Sequence Data Phylogeny polymerase chain reaction ribosomal RNA RNA, Ribosomal, 16S - genetics sequence analysis Swine Taxonomy Thermoplasma volcanium
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