The Interaction Between Intrathecal Administration of Low Doses of Palmitoylethanolamide and AM251 in Formalin-Induced Pain Related Behavior and Spinal Cord IL1-β Expression in Rats
Most of the modulating effects of cannabinoids on pain are through putative cannabinoid CB1 and CB2 receptors. However, the involvement of other receptors is also suggested. Cannabinoid compounds with analgesic activity such as palmitoylethanolamide (PEA) show low affinity to CB1 and CB2 receptors,...
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| Veröffentlicht in: | Neurochemical research 2012-04, Vol.37 (4), p.778-785 |
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| creator | Naderi, Nima Majidi, Mohsen Mousavi, Zahra Khoramian Tusi, Solaleh Mansouri, Zahra Khodagholi, Fariba |
| description | Most of the modulating effects of cannabinoids on pain are through putative cannabinoid CB1 and CB2 receptors. However, the involvement of other receptors is also suggested. Cannabinoid compounds with analgesic activity such as palmitoylethanolamide (PEA) show low affinity to CB1 and CB2 receptors, yet selectively activate GPR55 receptors. The objective of the present study was to evaluate the possible role of spinal CB1 and GPR55 receptors on antinociceptive activity of PEA in formalin test as well as in the spinal expression of IL1-β in rat. Intrathecal (i.t.) administration of PEA (1, 10 μg) significantly decreased both pain-related scores in formalin test and IL1-β expression in rat spinal cord. Pretreatment of rats with low doses of CB1 receptor antagonist/GPR55 receptor agonist AM251 (10, 100 ng; i.t.), did not attenuated the effect of PEA, yet even significantly increased the effect of PEA on IL1-β expression in rat spinal cord. Interestingly, i.t. administration of low doses of AM251 per se significantly decreased both pain related behavior and spinal IL1-β expression in formalin test. These findings suggest the possible involvement of receptors other than CB1 receptors in spinal pain pathways, such as GPR55, in pain modulating activity of cannabinoids. |
| doi_str_mv | 10.1007/s11064-011-0672-2 |
| format | Article |
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However, the involvement of other receptors is also suggested. Cannabinoid compounds with analgesic activity such as palmitoylethanolamide (PEA) show low affinity to CB1 and CB2 receptors, yet selectively activate GPR55 receptors. The objective of the present study was to evaluate the possible role of spinal CB1 and GPR55 receptors on antinociceptive activity of PEA in formalin test as well as in the spinal expression of IL1-β in rat. Intrathecal (i.t.) administration of PEA (1, 10 μg) significantly decreased both pain-related scores in formalin test and IL1-β expression in rat spinal cord. Pretreatment of rats with low doses of CB1 receptor antagonist/GPR55 receptor agonist AM251 (10, 100 ng; i.t.), did not attenuated the effect of PEA, yet even significantly increased the effect of PEA on IL1-β expression in rat spinal cord. Interestingly, i.t. administration of low doses of AM251 per se significantly decreased both pain related behavior and spinal IL1-β expression in formalin test. These findings suggest the possible involvement of receptors other than CB1 receptors in spinal pain pathways, such as GPR55, in pain modulating activity of cannabinoids.</description><identifier>ISSN: 0364-3190</identifier><identifier>EISSN: 1573-6903</identifier><identifier>DOI: 10.1007/s11064-011-0672-2</identifier><identifier>PMID: 22201038</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject><![CDATA[Analgesics ; Animals ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Cannabinoid CB1 receptors ; Cannabinoid CB2 receptors ; Cell Biology ; Drug Interactions - physiology ; Endocannabinoids - administration & dosage ; Endocannabinoids - metabolism ; Ethanolamines - administration & dosage ; Ethanolamines - metabolism ; Injections, Spinal ; Interleukin 1 ; Interleukin-1beta - biosynthesis ; Male ; Neurochemistry ; Neurology ; Neurosciences ; Original Paper ; Pain ; Pain - drug therapy ; Pain - metabolism ; Pain Measurement - drug effects ; Pain perception ; Palmitic Acids - administration & dosage ; Palmitic Acids - metabolism ; palmitoylethanolamide ; Piperidines - administration & dosage ; Piperidines - metabolism ; Pyrazoles - administration & dosage ; Pyrazoles - metabolism ; Rats ; Rats, Wistar ; Receptor, Cannabinoid, CB1 - antagonists & inhibitors ; Receptor, Cannabinoid, CB1 - metabolism ; Receptors, Cannabinoid - metabolism ; Receptors, G-Protein-Coupled - agonists ; Receptors, G-Protein-Coupled - metabolism ; Spinal cord ; Spinal Cord - drug effects ; Spinal Cord - metabolism]]></subject><ispartof>Neurochemical research, 2012-04, Vol.37 (4), p.778-785</ispartof><rights>Springer Science+Business Media, LLC 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-fd64d9d32f91475438499851a2b64c18a9ffb5499030af4db81ce4e3a0a48f363</citedby><cites>FETCH-LOGICAL-c375t-fd64d9d32f91475438499851a2b64c18a9ffb5499030af4db81ce4e3a0a48f363</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11064-011-0672-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11064-011-0672-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22201038$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Naderi, Nima</creatorcontrib><creatorcontrib>Majidi, Mohsen</creatorcontrib><creatorcontrib>Mousavi, Zahra</creatorcontrib><creatorcontrib>Khoramian Tusi, Solaleh</creatorcontrib><creatorcontrib>Mansouri, Zahra</creatorcontrib><creatorcontrib>Khodagholi, Fariba</creatorcontrib><title>The Interaction Between Intrathecal Administration of Low Doses of Palmitoylethanolamide and AM251 in Formalin-Induced Pain Related Behavior and Spinal Cord IL1-β Expression in Rats</title><title>Neurochemical research</title><addtitle>Neurochem Res</addtitle><addtitle>Neurochem Res</addtitle><description>Most of the modulating effects of cannabinoids on pain are through putative cannabinoid CB1 and CB2 receptors. However, the involvement of other receptors is also suggested. Cannabinoid compounds with analgesic activity such as palmitoylethanolamide (PEA) show low affinity to CB1 and CB2 receptors, yet selectively activate GPR55 receptors. The objective of the present study was to evaluate the possible role of spinal CB1 and GPR55 receptors on antinociceptive activity of PEA in formalin test as well as in the spinal expression of IL1-β in rat. Intrathecal (i.t.) administration of PEA (1, 10 μg) significantly decreased both pain-related scores in formalin test and IL1-β expression in rat spinal cord. Pretreatment of rats with low doses of CB1 receptor antagonist/GPR55 receptor agonist AM251 (10, 100 ng; i.t.), did not attenuated the effect of PEA, yet even significantly increased the effect of PEA on IL1-β expression in rat spinal cord. Interestingly, i.t. administration of low doses of AM251 per se significantly decreased both pain related behavior and spinal IL1-β expression in formalin test. These findings suggest the possible involvement of receptors other than CB1 receptors in spinal pain pathways, such as GPR55, in pain modulating activity of cannabinoids.</description><subject>Analgesics</subject><subject>Animals</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cannabinoid CB1 receptors</subject><subject>Cannabinoid CB2 receptors</subject><subject>Cell Biology</subject><subject>Drug Interactions - physiology</subject><subject>Endocannabinoids - administration & dosage</subject><subject>Endocannabinoids - metabolism</subject><subject>Ethanolamines - administration & dosage</subject><subject>Ethanolamines - metabolism</subject><subject>Injections, Spinal</subject><subject>Interleukin 1</subject><subject>Interleukin-1beta - biosynthesis</subject><subject>Male</subject><subject>Neurochemistry</subject><subject>Neurology</subject><subject>Neurosciences</subject><subject>Original Paper</subject><subject>Pain</subject><subject>Pain - drug therapy</subject><subject>Pain - metabolism</subject><subject>Pain Measurement - drug effects</subject><subject>Pain perception</subject><subject>Palmitic Acids - administration & dosage</subject><subject>Palmitic Acids - metabolism</subject><subject>palmitoylethanolamide</subject><subject>Piperidines - administration & dosage</subject><subject>Piperidines - metabolism</subject><subject>Pyrazoles - administration & dosage</subject><subject>Pyrazoles - metabolism</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Receptor, Cannabinoid, CB1 - antagonists & inhibitors</subject><subject>Receptor, Cannabinoid, CB1 - metabolism</subject><subject>Receptors, Cannabinoid - metabolism</subject><subject>Receptors, G-Protein-Coupled - agonists</subject><subject>Receptors, G-Protein-Coupled - metabolism</subject><subject>Spinal cord</subject><subject>Spinal Cord - drug effects</subject><subject>Spinal Cord - metabolism</subject><issn>0364-3190</issn><issn>1573-6903</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc9uEzEQxi0EomnhAbgg3zgteOz9e0zTFiIFgaCcrcl6lrjatYPtUPpaHPoYPBNeUjhysueb7zcjzcfYCxCvQYjmTQQQdVkIgELUjSzkI7aAqlFF3Qn1mC2Eyl0FnThhpzHeCJEpCU_ZiZQy_1W7YPfXO-Jrlyhgn6x3_JzSLZGbtYBpRz2OfGkm62ychdniB77xt_zCR4pz8RHHySZ_N1LaofMjTtYQR2f48r2sgFvHr3yYcLSuWDtz6MlkJqufaMSUi3Pa4Xfrwx_m8966vHPlg-HrDRS_fvLLH_tAMc67ZwpTfMaeDDhGev7wnrEvV5fXq3fF5sPb9Wq5KXrVVKkYTF2azig5dFA2VanasuvaClBu67KHFrth2FZZE0rgUJptCz2VpFBg2Q6qVmfs1XHuPvhvB4pJTzb2NI7oyB-i7uoW6qbuIDvh6OyDjzHQoPfBThjuNAg9p6WPaemclp7T0jIzLx-mH7YTmX_E33iyQR4NMbfcVwr6xh9CPk_8z9Tf3Sahhg</recordid><startdate>20120401</startdate><enddate>20120401</enddate><creator>Naderi, Nima</creator><creator>Majidi, Mohsen</creator><creator>Mousavi, Zahra</creator><creator>Khoramian Tusi, Solaleh</creator><creator>Mansouri, Zahra</creator><creator>Khodagholi, Fariba</creator><general>Springer US</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>20120401</creationdate><title>The Interaction Between Intrathecal Administration of Low Doses of Palmitoylethanolamide and AM251 in Formalin-Induced Pain Related Behavior and Spinal Cord IL1-β Expression in Rats</title><author>Naderi, Nima ; Majidi, Mohsen ; Mousavi, Zahra ; Khoramian Tusi, Solaleh ; Mansouri, Zahra ; Khodagholi, Fariba</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-fd64d9d32f91475438499851a2b64c18a9ffb5499030af4db81ce4e3a0a48f363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Analgesics</topic><topic>Animals</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cannabinoid CB1 receptors</topic><topic>Cannabinoid CB2 receptors</topic><topic>Cell Biology</topic><topic>Drug Interactions - physiology</topic><topic>Endocannabinoids - administration & dosage</topic><topic>Endocannabinoids - metabolism</topic><topic>Ethanolamines - administration & dosage</topic><topic>Ethanolamines - metabolism</topic><topic>Injections, Spinal</topic><topic>Interleukin 1</topic><topic>Interleukin-1beta - biosynthesis</topic><topic>Male</topic><topic>Neurochemistry</topic><topic>Neurology</topic><topic>Neurosciences</topic><topic>Original Paper</topic><topic>Pain</topic><topic>Pain - drug therapy</topic><topic>Pain - metabolism</topic><topic>Pain Measurement - drug effects</topic><topic>Pain perception</topic><topic>Palmitic Acids - administration & dosage</topic><topic>Palmitic Acids - metabolism</topic><topic>palmitoylethanolamide</topic><topic>Piperidines - administration & dosage</topic><topic>Piperidines - metabolism</topic><topic>Pyrazoles - administration & dosage</topic><topic>Pyrazoles - metabolism</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Receptor, Cannabinoid, CB1 - antagonists & inhibitors</topic><topic>Receptor, Cannabinoid, CB1 - metabolism</topic><topic>Receptors, Cannabinoid - metabolism</topic><topic>Receptors, G-Protein-Coupled - agonists</topic><topic>Receptors, G-Protein-Coupled - metabolism</topic><topic>Spinal cord</topic><topic>Spinal Cord - drug effects</topic><topic>Spinal Cord - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Naderi, Nima</creatorcontrib><creatorcontrib>Majidi, Mohsen</creatorcontrib><creatorcontrib>Mousavi, Zahra</creatorcontrib><creatorcontrib>Khoramian Tusi, Solaleh</creatorcontrib><creatorcontrib>Mansouri, Zahra</creatorcontrib><creatorcontrib>Khodagholi, Fariba</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Neurochemical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Naderi, Nima</au><au>Majidi, Mohsen</au><au>Mousavi, Zahra</au><au>Khoramian Tusi, Solaleh</au><au>Mansouri, Zahra</au><au>Khodagholi, Fariba</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Interaction Between Intrathecal Administration of Low Doses of Palmitoylethanolamide and AM251 in Formalin-Induced Pain Related Behavior and Spinal Cord IL1-β Expression in Rats</atitle><jtitle>Neurochemical research</jtitle><stitle>Neurochem Res</stitle><addtitle>Neurochem Res</addtitle><date>2012-04-01</date><risdate>2012</risdate><volume>37</volume><issue>4</issue><spage>778</spage><epage>785</epage><pages>778-785</pages><issn>0364-3190</issn><eissn>1573-6903</eissn><abstract>Most of the modulating effects of cannabinoids on pain are through putative cannabinoid CB1 and CB2 receptors. However, the involvement of other receptors is also suggested. Cannabinoid compounds with analgesic activity such as palmitoylethanolamide (PEA) show low affinity to CB1 and CB2 receptors, yet selectively activate GPR55 receptors. The objective of the present study was to evaluate the possible role of spinal CB1 and GPR55 receptors on antinociceptive activity of PEA in formalin test as well as in the spinal expression of IL1-β in rat. Intrathecal (i.t.) administration of PEA (1, 10 μg) significantly decreased both pain-related scores in formalin test and IL1-β expression in rat spinal cord. Pretreatment of rats with low doses of CB1 receptor antagonist/GPR55 receptor agonist AM251 (10, 100 ng; i.t.), did not attenuated the effect of PEA, yet even significantly increased the effect of PEA on IL1-β expression in rat spinal cord. Interestingly, i.t. administration of low doses of AM251 per se significantly decreased both pain related behavior and spinal IL1-β expression in formalin test. These findings suggest the possible involvement of receptors other than CB1 receptors in spinal pain pathways, such as GPR55, in pain modulating activity of cannabinoids.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>22201038</pmid><doi>10.1007/s11064-011-0672-2</doi><tpages>8</tpages></addata></record> |
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| ispartof | Neurochemical research, 2012-04, Vol.37 (4), p.778-785 |
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| subjects | Analgesics Animals Biochemistry Biomedical and Life Sciences Biomedicine Cannabinoid CB1 receptors Cannabinoid CB2 receptors Cell Biology Drug Interactions - physiology Endocannabinoids - administration & dosage Endocannabinoids - metabolism Ethanolamines - administration & dosage Ethanolamines - metabolism Injections, Spinal Interleukin 1 Interleukin-1beta - biosynthesis Male Neurochemistry Neurology Neurosciences Original Paper Pain Pain - drug therapy Pain - metabolism Pain Measurement - drug effects Pain perception Palmitic Acids - administration & dosage Palmitic Acids - metabolism palmitoylethanolamide Piperidines - administration & dosage Piperidines - metabolism Pyrazoles - administration & dosage Pyrazoles - metabolism Rats Rats, Wistar Receptor, Cannabinoid, CB1 - antagonists & inhibitors Receptor, Cannabinoid, CB1 - metabolism Receptors, Cannabinoid - metabolism Receptors, G-Protein-Coupled - agonists Receptors, G-Protein-Coupled - metabolism Spinal cord Spinal Cord - drug effects Spinal Cord - metabolism |
| title | The Interaction Between Intrathecal Administration of Low Doses of Palmitoylethanolamide and AM251 in Formalin-Induced Pain Related Behavior and Spinal Cord IL1-β Expression in Rats |
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