Genotyping of Bacillus anthracis vaccine strains by multilocus VNTR analysis
In the course of this investigation, we described the genotypic variations across 18 variable chromosomal loci of the five known anthrax bacillus vaccine strains from the collection of microbial cultures of the 48th Central Research Institute of the Ministry of Defense of the Russian Federation. The...
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| Veröffentlicht in: | Molecular genetics, microbiology and virology microbiology and virology, 2009-12, Vol.24 (4), p.177-182 |
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| container_title | Molecular genetics, microbiology and virology |
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| creator | Kuznetsovskii, A. V. Migova, L. V. Fedorov, Yu. N. Kibirev, Ya. A. Vorob’ev, A. A. Darmov, I. V. Borisevich, I. V. Savinykh, A. V. Gorin, O. V. |
| description | In the course of this investigation, we described the genotypic variations across 18 variable chromosomal loci of the five known anthrax bacillus vaccine strains from the collection of microbial cultures of the 48th Central Research Institute of the Ministry of Defense of the Russian Federation. The persistence of inheriting VNTR loci and their potential application in gene identification were estimated in closely related
B. anthracis
strains. The genetic profile of each of the studied vaccine strains has been determined for all 18 polymorphic loci. Sequencing of their amplification products was performed. We have confirmed that the variations in the electrophoretic mobility of the amplicons are due to the presence of repeats with different numbers of copies in their structure. |
| doi_str_mv | 10.3103/S089141680904003X |
| format | Article |
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B. anthracis
strains. The genetic profile of each of the studied vaccine strains has been determined for all 18 polymorphic loci. Sequencing of their amplification products was performed. We have confirmed that the variations in the electrophoretic mobility of the amplicons are due to the presence of repeats with different numbers of copies in their structure.</description><identifier>ISSN: 0891-4168</identifier><identifier>EISSN: 1934-841X</identifier><identifier>DOI: 10.3103/S089141680904003X</identifier><language>eng</language><publisher>Heidelberg: Allerton Press, Inc</publisher><subject>Anthrax ; Bacillus anthracis ; Biomedical and Life Sciences ; Experimental Works ; Life Sciences ; Microbiology ; Molecular Medicine</subject><ispartof>Molecular genetics, microbiology and virology, 2009-12, Vol.24 (4), p.177-182</ispartof><rights>Allerton Press, Inc. 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c320t-d967d455d26ccac7330c03cc2714f8e0cc3535ee84d20825885843d5e61216a3</citedby><cites>FETCH-LOGICAL-c320t-d967d455d26ccac7330c03cc2714f8e0cc3535ee84d20825885843d5e61216a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.3103/S089141680904003X$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.3103/S089141680904003X$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27922,27923,41486,42555,51317</link.rule.ids></links><search><creatorcontrib>Kuznetsovskii, A. V.</creatorcontrib><creatorcontrib>Migova, L. V.</creatorcontrib><creatorcontrib>Fedorov, Yu. N.</creatorcontrib><creatorcontrib>Kibirev, Ya. A.</creatorcontrib><creatorcontrib>Vorob’ev, A. A.</creatorcontrib><creatorcontrib>Darmov, I. V.</creatorcontrib><creatorcontrib>Borisevich, I. V.</creatorcontrib><creatorcontrib>Savinykh, A. V.</creatorcontrib><creatorcontrib>Gorin, O. V.</creatorcontrib><title>Genotyping of Bacillus anthracis vaccine strains by multilocus VNTR analysis</title><title>Molecular genetics, microbiology and virology</title><addtitle>Mol. Genet. Microbiol. Virol</addtitle><description>In the course of this investigation, we described the genotypic variations across 18 variable chromosomal loci of the five known anthrax bacillus vaccine strains from the collection of microbial cultures of the 48th Central Research Institute of the Ministry of Defense of the Russian Federation. The persistence of inheriting VNTR loci and their potential application in gene identification were estimated in closely related
B. anthracis
strains. The genetic profile of each of the studied vaccine strains has been determined for all 18 polymorphic loci. Sequencing of their amplification products was performed. We have confirmed that the variations in the electrophoretic mobility of the amplicons are due to the presence of repeats with different numbers of copies in their structure.</description><subject>Anthrax</subject><subject>Bacillus anthracis</subject><subject>Biomedical and Life Sciences</subject><subject>Experimental Works</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Molecular Medicine</subject><issn>0891-4168</issn><issn>1934-841X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNp9kE1Lw0AQhhdRsFZ_gLfcPEVnP7M5atEqFAUt0ltYN5u6ZbupO4mQf29KvQmehuF9noF5CbmkcM0p8Js30CUVVGkoQQDw1RGZ0JKLXAu6OiaTfZzv81NyhrgBkIpxNSGLuYttN-x8XGdtk90Z60PoMTOx-0zjgtm3sdZHl2GXjI-YfQzZtg-dD60duffn5esImzCgx3Ny0piA7uJ3Tsny4X45e8wXL_On2e0it5xBl9elKmohZc2UtcYWnIMFbi0rqGi0A2u55NI5LWoGmkmtpRa8lk5RRpXhU3J1OLtL7VfvsKu2Hq0LwUTX9liVSlNVQCFGkh5Im1rE5Jpql_zWpKGiUO17q_70Njrs4ODIxrVL1abt0_gh_iP9AIV2b0E</recordid><startdate>20091201</startdate><enddate>20091201</enddate><creator>Kuznetsovskii, A. V.</creator><creator>Migova, L. V.</creator><creator>Fedorov, Yu. N.</creator><creator>Kibirev, Ya. A.</creator><creator>Vorob’ev, A. A.</creator><creator>Darmov, I. V.</creator><creator>Borisevich, I. V.</creator><creator>Savinykh, A. V.</creator><creator>Gorin, O. V.</creator><general>Allerton Press, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20091201</creationdate><title>Genotyping of Bacillus anthracis vaccine strains by multilocus VNTR analysis</title><author>Kuznetsovskii, A. V. ; Migova, L. V. ; Fedorov, Yu. N. ; Kibirev, Ya. A. ; Vorob’ev, A. A. ; Darmov, I. V. ; Borisevich, I. V. ; Savinykh, A. V. ; Gorin, O. V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c320t-d967d455d26ccac7330c03cc2714f8e0cc3535ee84d20825885843d5e61216a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Anthrax</topic><topic>Bacillus anthracis</topic><topic>Biomedical and Life Sciences</topic><topic>Experimental Works</topic><topic>Life Sciences</topic><topic>Microbiology</topic><topic>Molecular Medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kuznetsovskii, A. V.</creatorcontrib><creatorcontrib>Migova, L. V.</creatorcontrib><creatorcontrib>Fedorov, Yu. N.</creatorcontrib><creatorcontrib>Kibirev, Ya. A.</creatorcontrib><creatorcontrib>Vorob’ev, A. A.</creatorcontrib><creatorcontrib>Darmov, I. V.</creatorcontrib><creatorcontrib>Borisevich, I. V.</creatorcontrib><creatorcontrib>Savinykh, A. V.</creatorcontrib><creatorcontrib>Gorin, O. V.</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Molecular genetics, microbiology and virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kuznetsovskii, A. V.</au><au>Migova, L. V.</au><au>Fedorov, Yu. N.</au><au>Kibirev, Ya. A.</au><au>Vorob’ev, A. A.</au><au>Darmov, I. V.</au><au>Borisevich, I. V.</au><au>Savinykh, A. V.</au><au>Gorin, O. V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genotyping of Bacillus anthracis vaccine strains by multilocus VNTR analysis</atitle><jtitle>Molecular genetics, microbiology and virology</jtitle><stitle>Mol. Genet. Microbiol. Virol</stitle><date>2009-12-01</date><risdate>2009</risdate><volume>24</volume><issue>4</issue><spage>177</spage><epage>182</epage><pages>177-182</pages><issn>0891-4168</issn><eissn>1934-841X</eissn><abstract>In the course of this investigation, we described the genotypic variations across 18 variable chromosomal loci of the five known anthrax bacillus vaccine strains from the collection of microbial cultures of the 48th Central Research Institute of the Ministry of Defense of the Russian Federation. The persistence of inheriting VNTR loci and their potential application in gene identification were estimated in closely related
B. anthracis
strains. The genetic profile of each of the studied vaccine strains has been determined for all 18 polymorphic loci. Sequencing of their amplification products was performed. We have confirmed that the variations in the electrophoretic mobility of the amplicons are due to the presence of repeats with different numbers of copies in their structure.</abstract><cop>Heidelberg</cop><pub>Allerton Press, Inc</pub><doi>10.3103/S089141680904003X</doi><tpages>6</tpages></addata></record> |
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| subjects | Anthrax Bacillus anthracis Biomedical and Life Sciences Experimental Works Life Sciences Microbiology Molecular Medicine |
| title | Genotyping of Bacillus anthracis vaccine strains by multilocus VNTR analysis |
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