Cathepsins B1 and B2 in the neuropathogenic schistosome Trichobilharzia regenti: distinct gene expression profiles and presumptive roles throughout the life cycle
The neurotropic bird schistosome Trichobilharzia regenti possesses papain-like cysteine peptidases which have also been shown to be crucial enzymes in various developmental stages of the related human parasites Schistosoma spp. In this paper, we present data obtained by real-time polymerase chain re...
Gespeichert in:
| Veröffentlicht in: | Parasitology research (1987) 2010-08, Vol.107 (3), p.751-755 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 755 |
|---|---|
| container_issue | 3 |
| container_start_page | 751 |
| container_title | Parasitology research (1987) |
| container_volume | 107 |
| creator | Dolečková, Kateřina Albrecht, Tomáš Mikeš, Libor Horák, Petr |
| description | The neurotropic bird schistosome Trichobilharzia regenti possesses papain-like cysteine peptidases which have also been shown to be crucial enzymes in various developmental stages of the related human parasites Schistosoma spp. In this paper, we present data obtained by real-time polymerase chain reaction on the temporal distribution of transcripts of two cathepsins in different developmental stages of T. regenti: cathepsin B1 originally described from the gut lumen of schistosomula with presumptive role in nutrient digestion and cathepsin B2 originally found in penetration glands of cercariae with probable involvement in invasion of the final host. In spite of their mutual resemblance at the sequence level, the mRNA expression profiles clearly show distinct expression of cathepsins B1 and B2 during the development from eggs to cercariae. In the case of both cathepsins, the highest level of transcription was detected in intravertebrate stages. Putative functions of cathepsins B1 and B2 in schistosome developmental stages are discussed. |
| doi_str_mv | 10.1007/s00436-010-1943-6 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_968161902</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A381057007</galeid><sourcerecordid>A381057007</sourcerecordid><originalsourceid>FETCH-LOGICAL-c438t-36e5b2701aca3eba3d6de702a61752acdd51c940666e7259122dbd99dcd71f3c3</originalsourceid><addsrcrecordid>eNqFkcFu1DAQhiMEokvhAbiAb5xSPHZiJ9zaFQWkShxoz5bXnmxcJXawE0R5HJ60TlM4wsmeme-fGc1fFK-BngGl8n2itOKipEBLaCteiifFDirOclTXT4sdbfOfAvCT4kVKt5SCFFX1vDhhtK5FxZpd8Xuv5x6n5HwiF0C0t-SCEedJzhKPSwxTBsIRvTMkmd6lOaQwIrmOzvTh4IZex19Ok4iZmd0HYjPivJlJjpHgzyliSi54MsXQuQHTw5A1u4zT7H4giWHNzn0My7EPy_wwe3AdEnNnBnxZPOv0kPDV43ta3Fx-vN5_Lq--fvqyP78qTcWbueQC6wOTFLTRHA-aW2FRUqYFyJppY20Npq2oEAIlq1tgzB5s21pjJXTc8NPi3dY3L_p9wTSr0SWDw6A9hiWpVjQgoKXsv6SsmpZJYG0mzzbyqAdUzndhjnk_oy2OzgSP60XUOW-A1jJbmgWwCUwMKUXs1BTdqOOdAqpW09VmuqJrnE1XImvePK6zHEa0fxV_XM4A24CUS_6IUd2GJfp8zH92fbuJOh2UPkaX1M03RoFTaKQAXvN7X43Dvw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>748927129</pqid></control><display><type>article</type><title>Cathepsins B1 and B2 in the neuropathogenic schistosome Trichobilharzia regenti: distinct gene expression profiles and presumptive roles throughout the life cycle</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Dolečková, Kateřina ; Albrecht, Tomáš ; Mikeš, Libor ; Horák, Petr</creator><creatorcontrib>Dolečková, Kateřina ; Albrecht, Tomáš ; Mikeš, Libor ; Horák, Petr</creatorcontrib><description>The neurotropic bird schistosome Trichobilharzia regenti possesses papain-like cysteine peptidases which have also been shown to be crucial enzymes in various developmental stages of the related human parasites Schistosoma spp. In this paper, we present data obtained by real-time polymerase chain reaction on the temporal distribution of transcripts of two cathepsins in different developmental stages of T. regenti: cathepsin B1 originally described from the gut lumen of schistosomula with presumptive role in nutrient digestion and cathepsin B2 originally found in penetration glands of cercariae with probable involvement in invasion of the final host. In spite of their mutual resemblance at the sequence level, the mRNA expression profiles clearly show distinct expression of cathepsins B1 and B2 during the development from eggs to cercariae. In the case of both cathepsins, the highest level of transcription was detected in intravertebrate stages. Putative functions of cathepsins B1 and B2 in schistosome developmental stages are discussed.</description><identifier>ISSN: 0932-0113</identifier><identifier>EISSN: 1432-1955</identifier><identifier>DOI: 10.1007/s00436-010-1943-6</identifier><identifier>PMID: 20556428</identifier><language>eng</language><publisher>Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Animals ; Biomedical and Life Sciences ; Biomedicine ; Cathepsin B - genetics ; Cathepsin B - metabolism ; Cathepsins ; Cysteine ; Ducks ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation, Developmental ; Helminth Proteins - genetics ; Helminth Proteins - metabolism ; Immunology ; Isoenzymes - genetics ; Isoenzymes - metabolism ; Life Cycle Stages ; Medical Microbiology ; Microbiology ; Papain ; RNA ; Schistosoma ; Schistosomatidae - enzymology ; Schistosomatidae - genetics ; Schistosomatidae - growth & development ; Short Communication ; Snails ; Trichobilharzia regenti</subject><ispartof>Parasitology research (1987), 2010-08, Vol.107 (3), p.751-755</ispartof><rights>Springer-Verlag 2010</rights><rights>COPYRIGHT 2010 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-36e5b2701aca3eba3d6de702a61752acdd51c940666e7259122dbd99dcd71f3c3</citedby><cites>FETCH-LOGICAL-c438t-36e5b2701aca3eba3d6de702a61752acdd51c940666e7259122dbd99dcd71f3c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00436-010-1943-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00436-010-1943-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20556428$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dolečková, Kateřina</creatorcontrib><creatorcontrib>Albrecht, Tomáš</creatorcontrib><creatorcontrib>Mikeš, Libor</creatorcontrib><creatorcontrib>Horák, Petr</creatorcontrib><title>Cathepsins B1 and B2 in the neuropathogenic schistosome Trichobilharzia regenti: distinct gene expression profiles and presumptive roles throughout the life cycle</title><title>Parasitology research (1987)</title><addtitle>Parasitol Res</addtitle><addtitle>Parasitol Res</addtitle><description>The neurotropic bird schistosome Trichobilharzia regenti possesses papain-like cysteine peptidases which have also been shown to be crucial enzymes in various developmental stages of the related human parasites Schistosoma spp. In this paper, we present data obtained by real-time polymerase chain reaction on the temporal distribution of transcripts of two cathepsins in different developmental stages of T. regenti: cathepsin B1 originally described from the gut lumen of schistosomula with presumptive role in nutrient digestion and cathepsin B2 originally found in penetration glands of cercariae with probable involvement in invasion of the final host. In spite of their mutual resemblance at the sequence level, the mRNA expression profiles clearly show distinct expression of cathepsins B1 and B2 during the development from eggs to cercariae. In the case of both cathepsins, the highest level of transcription was detected in intravertebrate stages. Putative functions of cathepsins B1 and B2 in schistosome developmental stages are discussed.</description><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cathepsin B - genetics</subject><subject>Cathepsin B - metabolism</subject><subject>Cathepsins</subject><subject>Cysteine</subject><subject>Ducks</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Helminth Proteins - genetics</subject><subject>Helminth Proteins - metabolism</subject><subject>Immunology</subject><subject>Isoenzymes - genetics</subject><subject>Isoenzymes - metabolism</subject><subject>Life Cycle Stages</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Papain</subject><subject>RNA</subject><subject>Schistosoma</subject><subject>Schistosomatidae - enzymology</subject><subject>Schistosomatidae - genetics</subject><subject>Schistosomatidae - growth & development</subject><subject>Short Communication</subject><subject>Snails</subject><subject>Trichobilharzia regenti</subject><issn>0932-0113</issn><issn>1432-1955</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhiMEokvhAbiAb5xSPHZiJ9zaFQWkShxoz5bXnmxcJXawE0R5HJ60TlM4wsmeme-fGc1fFK-BngGl8n2itOKipEBLaCteiifFDirOclTXT4sdbfOfAvCT4kVKt5SCFFX1vDhhtK5FxZpd8Xuv5x6n5HwiF0C0t-SCEedJzhKPSwxTBsIRvTMkmd6lOaQwIrmOzvTh4IZex19Ok4iZmd0HYjPivJlJjpHgzyliSi54MsXQuQHTw5A1u4zT7H4giWHNzn0My7EPy_wwe3AdEnNnBnxZPOv0kPDV43ta3Fx-vN5_Lq--fvqyP78qTcWbueQC6wOTFLTRHA-aW2FRUqYFyJppY20Npq2oEAIlq1tgzB5s21pjJXTc8NPi3dY3L_p9wTSr0SWDw6A9hiWpVjQgoKXsv6SsmpZJYG0mzzbyqAdUzndhjnk_oy2OzgSP60XUOW-A1jJbmgWwCUwMKUXs1BTdqOOdAqpW09VmuqJrnE1XImvePK6zHEa0fxV_XM4A24CUS_6IUd2GJfp8zH92fbuJOh2UPkaX1M03RoFTaKQAXvN7X43Dvw</recordid><startdate>20100801</startdate><enddate>20100801</enddate><creator>Dolečková, Kateřina</creator><creator>Albrecht, Tomáš</creator><creator>Mikeš, Libor</creator><creator>Horák, Petr</creator><general>Berlin/Heidelberg : Springer-Verlag</general><general>Springer-Verlag</general><general>Springer</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SN</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20100801</creationdate><title>Cathepsins B1 and B2 in the neuropathogenic schistosome Trichobilharzia regenti: distinct gene expression profiles and presumptive roles throughout the life cycle</title><author>Dolečková, Kateřina ; Albrecht, Tomáš ; Mikeš, Libor ; Horák, Petr</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-36e5b2701aca3eba3d6de702a61752acdd51c940666e7259122dbd99dcd71f3c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cathepsin B - genetics</topic><topic>Cathepsin B - metabolism</topic><topic>Cathepsins</topic><topic>Cysteine</topic><topic>Ducks</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Helminth Proteins - genetics</topic><topic>Helminth Proteins - metabolism</topic><topic>Immunology</topic><topic>Isoenzymes - genetics</topic><topic>Isoenzymes - metabolism</topic><topic>Life Cycle Stages</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Papain</topic><topic>RNA</topic><topic>Schistosoma</topic><topic>Schistosomatidae - enzymology</topic><topic>Schistosomatidae - genetics</topic><topic>Schistosomatidae - growth & development</topic><topic>Short Communication</topic><topic>Snails</topic><topic>Trichobilharzia regenti</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dolečková, Kateřina</creatorcontrib><creatorcontrib>Albrecht, Tomáš</creatorcontrib><creatorcontrib>Mikeš, Libor</creatorcontrib><creatorcontrib>Horák, Petr</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Ecology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Parasitology research (1987)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dolečková, Kateřina</au><au>Albrecht, Tomáš</au><au>Mikeš, Libor</au><au>Horák, Petr</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cathepsins B1 and B2 in the neuropathogenic schistosome Trichobilharzia regenti: distinct gene expression profiles and presumptive roles throughout the life cycle</atitle><jtitle>Parasitology research (1987)</jtitle><stitle>Parasitol Res</stitle><addtitle>Parasitol Res</addtitle><date>2010-08-01</date><risdate>2010</risdate><volume>107</volume><issue>3</issue><spage>751</spage><epage>755</epage><pages>751-755</pages><issn>0932-0113</issn><eissn>1432-1955</eissn><abstract>The neurotropic bird schistosome Trichobilharzia regenti possesses papain-like cysteine peptidases which have also been shown to be crucial enzymes in various developmental stages of the related human parasites Schistosoma spp. In this paper, we present data obtained by real-time polymerase chain reaction on the temporal distribution of transcripts of two cathepsins in different developmental stages of T. regenti: cathepsin B1 originally described from the gut lumen of schistosomula with presumptive role in nutrient digestion and cathepsin B2 originally found in penetration glands of cercariae with probable involvement in invasion of the final host. In spite of their mutual resemblance at the sequence level, the mRNA expression profiles clearly show distinct expression of cathepsins B1 and B2 during the development from eggs to cercariae. In the case of both cathepsins, the highest level of transcription was detected in intravertebrate stages. Putative functions of cathepsins B1 and B2 in schistosome developmental stages are discussed.</abstract><cop>Berlin/Heidelberg</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>20556428</pmid><doi>10.1007/s00436-010-1943-6</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0932-0113 |
| ispartof | Parasitology research (1987), 2010-08, Vol.107 (3), p.751-755 |
| issn | 0932-0113 1432-1955 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_968161902 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Animals Biomedical and Life Sciences Biomedicine Cathepsin B - genetics Cathepsin B - metabolism Cathepsins Cysteine Ducks Gene expression Gene Expression Profiling Gene Expression Regulation, Developmental Helminth Proteins - genetics Helminth Proteins - metabolism Immunology Isoenzymes - genetics Isoenzymes - metabolism Life Cycle Stages Medical Microbiology Microbiology Papain RNA Schistosoma Schistosomatidae - enzymology Schistosomatidae - genetics Schistosomatidae - growth & development Short Communication Snails Trichobilharzia regenti |
| title | Cathepsins B1 and B2 in the neuropathogenic schistosome Trichobilharzia regenti: distinct gene expression profiles and presumptive roles throughout the life cycle |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T04%3A38%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cathepsins%20B1%20and%20B2%20in%20the%20neuropathogenic%20schistosome%20Trichobilharzia%20regenti:%20distinct%20gene%20expression%20profiles%20and%20presumptive%20roles%20throughout%20the%20life%20cycle&rft.jtitle=Parasitology%20research%20(1987)&rft.au=Dole%C4%8Dkov%C3%A1,%20Kate%C5%99ina&rft.date=2010-08-01&rft.volume=107&rft.issue=3&rft.spage=751&rft.epage=755&rft.pages=751-755&rft.issn=0932-0113&rft.eissn=1432-1955&rft_id=info:doi/10.1007/s00436-010-1943-6&rft_dat=%3Cgale_proqu%3EA381057007%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=748927129&rft_id=info:pmid/20556428&rft_galeid=A381057007&rfr_iscdi=true |