Molecular cloning and phylogenetic analysis of small GTPase protein Tscdc42 from Trichinella spiralis
A full-length of complementary deoxyribonucleic acid ( Tscdc42 ) encoding a putative Rho-family small GTPase gene cdc42 was isolated from Trichinella spiralis, an economically important parasitic nematode of zoonosis. The uninterrupted open reading frame of TsCDC42 encodes a predicted protein of 147...
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| Veröffentlicht in: | Parasitology research (1987) 2010-03, Vol.106 (4), p.801-808 |
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| creator | Yang, Yurong Jian, Wei Qin, Weiwen |
| description | A full-length of complementary deoxyribonucleic acid (
Tscdc42
) encoding a putative Rho-family small GTPase gene
cdc42
was isolated from
Trichinella spiralis,
an economically important parasitic nematode of zoonosis. The uninterrupted open reading frame of TsCDC42 encodes a predicted protein of 147 amino acids and containing a highly conserved domain of CDC42. Comparison with selected sequences from the free-living nematode
Caenorhabditis elegans
,
Drosophila melanogaster
,
Xenopus laevis, Danio rerio
,
Mus musculus
, and human showed that Ts
cdc42
is highly conserved. The highest identity of TsCDC42 with CDC42 from
Drosophila
is 67%, the similarity is up to 73%, the identity of TsCDC42 with the CDC42 homologue of
C. elegans
is 62%, and the similarity is up to 71%. Phylogenetic analysis of the amino acid sequence data, using the neighbor-joining and maximum parsimony methods, revealed that TsCDC42 is closely related to the molecule inferred from the cdc-42 gene of
C. elegans
. The transcript of TsCDC42 was analyzed during different stages of the worm. |
| doi_str_mv | 10.1007/s00436-010-1735-z |
| format | Article |
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Tscdc42
) encoding a putative Rho-family small GTPase gene
cdc42
was isolated from
Trichinella spiralis,
an economically important parasitic nematode of zoonosis. The uninterrupted open reading frame of TsCDC42 encodes a predicted protein of 147 amino acids and containing a highly conserved domain of CDC42. Comparison with selected sequences from the free-living nematode
Caenorhabditis elegans
,
Drosophila melanogaster
,
Xenopus laevis, Danio rerio
,
Mus musculus
, and human showed that Ts
cdc42
is highly conserved. The highest identity of TsCDC42 with CDC42 from
Drosophila
is 67%, the similarity is up to 73%, the identity of TsCDC42 with the CDC42 homologue of
C. elegans
is 62%, and the similarity is up to 71%. Phylogenetic analysis of the amino acid sequence data, using the neighbor-joining and maximum parsimony methods, revealed that TsCDC42 is closely related to the molecule inferred from the cdc-42 gene of
C. elegans
. The transcript of TsCDC42 was analyzed during different stages of the worm.</description><identifier>ISSN: 0932-0113</identifier><identifier>EISSN: 1432-1955</identifier><identifier>DOI: 10.1007/s00436-010-1735-z</identifier><identifier>PMID: 20111874</identifier><identifier>CODEN: PARREZ</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Animals ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedicine ; Caenorhabditis elegans ; cdc42 GTP-Binding Protein - genetics ; Cloning ; Cloning, Molecular ; Cluster Analysis ; Danio rerio ; DNA, Helminth - chemistry ; DNA, Helminth - genetics ; Drosophila melanogaster ; Fundamental and applied biological sciences. Psychology ; General aspects ; General aspects and techniques. Study of several systematic groups. Models ; Genetic aspects ; Guanosine triphosphatase ; Health aspects ; Immunology ; Invertebrates ; Medical Microbiology ; Mice ; Microbial genetics ; Microbiology ; Molecular genetics ; Molecular Sequence Data ; Mus musculus ; Nematoda ; Original Paper ; Phylogeny ; Physiological aspects ; Polymorphism, Genetic ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; Trichinella spiralis ; Trichinella spiralis - genetics ; Xenopus laevis</subject><ispartof>Parasitology research (1987), 2010-03, Vol.106 (4), p.801-808</ispartof><rights>Springer-Verlag 2010</rights><rights>2015 INIST-CNRS</rights><rights>COPYRIGHT 2010 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c444t-8772a6bd40208f4f19cb08e0b408dabaeea3fc11ef29f0dc8c9473b633ca283f3</citedby><cites>FETCH-LOGICAL-c444t-8772a6bd40208f4f19cb08e0b408dabaeea3fc11ef29f0dc8c9473b633ca283f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00436-010-1735-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00436-010-1735-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22524592$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20111874$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Yurong</creatorcontrib><creatorcontrib>Jian, Wei</creatorcontrib><creatorcontrib>Qin, Weiwen</creatorcontrib><title>Molecular cloning and phylogenetic analysis of small GTPase protein Tscdc42 from Trichinella spiralis</title><title>Parasitology research (1987)</title><addtitle>Parasitol Res</addtitle><addtitle>Parasitol Res</addtitle><description>A full-length of complementary deoxyribonucleic acid (
Tscdc42
) encoding a putative Rho-family small GTPase gene
cdc42
was isolated from
Trichinella spiralis,
an economically important parasitic nematode of zoonosis. The uninterrupted open reading frame of TsCDC42 encodes a predicted protein of 147 amino acids and containing a highly conserved domain of CDC42. Comparison with selected sequences from the free-living nematode
Caenorhabditis elegans
,
Drosophila melanogaster
,
Xenopus laevis, Danio rerio
,
Mus musculus
, and human showed that Ts
cdc42
is highly conserved. The highest identity of TsCDC42 with CDC42 from
Drosophila
is 67%, the similarity is up to 73%, the identity of TsCDC42 with the CDC42 homologue of
C. elegans
is 62%, and the similarity is up to 71%. Phylogenetic analysis of the amino acid sequence data, using the neighbor-joining and maximum parsimony methods, revealed that TsCDC42 is closely related to the molecule inferred from the cdc-42 gene of
C. elegans
. The transcript of TsCDC42 was analyzed during different stages of the worm.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Caenorhabditis elegans</subject><subject>cdc42 GTP-Binding Protein - genetics</subject><subject>Cloning</subject><subject>Cloning, Molecular</subject><subject>Cluster Analysis</subject><subject>Danio rerio</subject><subject>DNA, Helminth - chemistry</subject><subject>DNA, Helminth - genetics</subject><subject>Drosophila melanogaster</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>General aspects and techniques. Study of several systematic groups. Models</subject><subject>Genetic aspects</subject><subject>Guanosine triphosphatase</subject><subject>Health aspects</subject><subject>Immunology</subject><subject>Invertebrates</subject><subject>Medical Microbiology</subject><subject>Mice</subject><subject>Microbial genetics</subject><subject>Microbiology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Mus musculus</subject><subject>Nematoda</subject><subject>Original Paper</subject><subject>Phylogeny</subject><subject>Physiological aspects</subject><subject>Polymorphism, Genetic</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><subject>Trichinella spiralis</subject><subject>Trichinella spiralis - genetics</subject><subject>Xenopus laevis</subject><issn>0932-0113</issn><issn>1432-1955</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS0EokvhA3BBviBOKeM_iZNjVZWCVASH5Ww5jr115diLZ3PYfnq8ygI3kA_2PP_ezEiPkLcMrhiA-ogAUnQNMGiYEm3z9IxsmBS8YUPbPicbGOobGBMX5BXiIwBTnZQvyQWvIuuV3BD3NUdnl2gKtTGnkHbUpInuH44x71xyh2CrYOIRA9LsKc4mRnq3_W7Q0X3JBxcS3aKdrOTUlzzTbQn2ISQXo6G4D8XEgK_JC28iujfn-5L8-HS7vfnc3H-7-3Jzfd9YKeWh6ZXiphsnCRx6Lz0b7Ai9g1FCP5nROGeEt4w5zwcPk-3tIJUYOyGs4b3w4pJ8WPvWzX4uDg96DmhPqySXF9RD17OOqWH4L6mEaIUaVFfJq5Xcmeh0SD4firH1TG4ONifnQ9WvBbS1LbS8GthqsCUjFuf1voTZlKNmoE-56TU3Dae65qafqufdeZ1lnN30x_E7qAq8PwMGrYm-mGQD_uV4y2U7nIbzlcP6lXau6Me8lBog_mP6L_28sNw</recordid><startdate>20100301</startdate><enddate>20100301</enddate><creator>Yang, Yurong</creator><creator>Jian, Wei</creator><creator>Qin, Weiwen</creator><general>Springer-Verlag</general><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20100301</creationdate><title>Molecular cloning and phylogenetic analysis of small GTPase protein Tscdc42 from Trichinella spiralis</title><author>Yang, Yurong ; Jian, Wei ; Qin, Weiwen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c444t-8772a6bd40208f4f19cb08e0b408dabaeea3fc11ef29f0dc8c9473b633ca283f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Caenorhabditis elegans</topic><topic>cdc42 GTP-Binding Protein - genetics</topic><topic>Cloning</topic><topic>Cloning, Molecular</topic><topic>Cluster Analysis</topic><topic>Danio rerio</topic><topic>DNA, Helminth - chemistry</topic><topic>DNA, Helminth - genetics</topic><topic>Drosophila melanogaster</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>General aspects and techniques. Study of several systematic groups. Models</topic><topic>Genetic aspects</topic><topic>Guanosine triphosphatase</topic><topic>Health aspects</topic><topic>Immunology</topic><topic>Invertebrates</topic><topic>Medical Microbiology</topic><topic>Mice</topic><topic>Microbial genetics</topic><topic>Microbiology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Mus musculus</topic><topic>Nematoda</topic><topic>Original Paper</topic><topic>Phylogeny</topic><topic>Physiological aspects</topic><topic>Polymorphism, Genetic</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><topic>Trichinella spiralis</topic><topic>Trichinella spiralis - genetics</topic><topic>Xenopus laevis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Yurong</creatorcontrib><creatorcontrib>Jian, Wei</creatorcontrib><creatorcontrib>Qin, Weiwen</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Parasitology research (1987)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Yurong</au><au>Jian, Wei</au><au>Qin, Weiwen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning and phylogenetic analysis of small GTPase protein Tscdc42 from Trichinella spiralis</atitle><jtitle>Parasitology research (1987)</jtitle><stitle>Parasitol Res</stitle><addtitle>Parasitol Res</addtitle><date>2010-03-01</date><risdate>2010</risdate><volume>106</volume><issue>4</issue><spage>801</spage><epage>808</epage><pages>801-808</pages><issn>0932-0113</issn><eissn>1432-1955</eissn><coden>PARREZ</coden><abstract>A full-length of complementary deoxyribonucleic acid (
Tscdc42
) encoding a putative Rho-family small GTPase gene
cdc42
was isolated from
Trichinella spiralis,
an economically important parasitic nematode of zoonosis. The uninterrupted open reading frame of TsCDC42 encodes a predicted protein of 147 amino acids and containing a highly conserved domain of CDC42. Comparison with selected sequences from the free-living nematode
Caenorhabditis elegans
,
Drosophila melanogaster
,
Xenopus laevis, Danio rerio
,
Mus musculus
, and human showed that Ts
cdc42
is highly conserved. The highest identity of TsCDC42 with CDC42 from
Drosophila
is 67%, the similarity is up to 73%, the identity of TsCDC42 with the CDC42 homologue of
C. elegans
is 62%, and the similarity is up to 71%. Phylogenetic analysis of the amino acid sequence data, using the neighbor-joining and maximum parsimony methods, revealed that TsCDC42 is closely related to the molecule inferred from the cdc-42 gene of
C. elegans
. The transcript of TsCDC42 was analyzed during different stages of the worm.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>20111874</pmid><doi>10.1007/s00436-010-1735-z</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0932-0113 |
| ispartof | Parasitology research (1987), 2010-03, Vol.106 (4), p.801-808 |
| issn | 0932-0113 1432-1955 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_968161799 |
| source | MEDLINE; SpringerNature Journals |
| subjects | Animals Biological and medical sciences Biomedical and Life Sciences Biomedicine Caenorhabditis elegans cdc42 GTP-Binding Protein - genetics Cloning Cloning, Molecular Cluster Analysis Danio rerio DNA, Helminth - chemistry DNA, Helminth - genetics Drosophila melanogaster Fundamental and applied biological sciences. Psychology General aspects General aspects and techniques. Study of several systematic groups. Models Genetic aspects Guanosine triphosphatase Health aspects Immunology Invertebrates Medical Microbiology Mice Microbial genetics Microbiology Molecular genetics Molecular Sequence Data Mus musculus Nematoda Original Paper Phylogeny Physiological aspects Polymorphism, Genetic Sequence Analysis, DNA Sequence Homology, Amino Acid Trichinella spiralis Trichinella spiralis - genetics Xenopus laevis |
| title | Molecular cloning and phylogenetic analysis of small GTPase protein Tscdc42 from Trichinella spiralis |
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