Effects of Recombinant Interleukin 1 and Interleukin 2 on Human Keratinocytes
The effects of recombinant interleukin 1 alpha and beta, as well as recombinant interleukin 2, on human keratinocyte proliferation were studied in serum-containing as well as defined media. Both interleukin 1 preparations did not stimulate keratinocyte growth; interleukin 2 also did not stimulate ke...
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| Veröffentlicht in: | Journal of investigative dermatology 1989-07, Vol.93 (1), p.121-126 |
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| container_title | Journal of investigative dermatology |
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| creator | Morhenn, Vera B Wastek, Gregory J Cua, Anastasia B Mansbridge, Jonathan N |
| description | The effects of recombinant interleukin 1 alpha and beta, as well as recombinant interleukin 2, on human keratinocyte proliferation were studied in serum-containing as well as defined media. Both interleukin 1 preparations did not stimulate keratinocyte growth; interleukin 2 also did not stimulate keratinocyte growth. To determine whether interleukin 1 beta binds to keratinocytes, a cell membrane assay was developed for these cells. Iodinated interleukin 1 beta binds to keratinocytes with a kD of 6.2nm and 2500 receptors per cell. To determine the effects of interleukin 1 beta on protein synthesis, the molecular patterns of radiolabeled cell extracts of interleukin 1 beta-treated and nontreated keratinocytes were compared using two-dimensional polyacrylamide gel electrophoresis. No significant changes in the molecular pattern of newly synthesized proteins were detected. Finally, none of these lymphokines induced HLA-DR expression by keratinocytes. |
| doi_str_mv | 10.1111/1523-1747.ep12277378 |
| format | Article |
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Both interleukin 1 preparations did not stimulate keratinocyte growth; interleukin 2 also did not stimulate keratinocyte growth. To determine whether interleukin 1 beta binds to keratinocytes, a cell membrane assay was developed for these cells. Iodinated interleukin 1 beta binds to keratinocytes with a kD of 6.2nm and 2500 receptors per cell. To determine the effects of interleukin 1 beta on protein synthesis, the molecular patterns of radiolabeled cell extracts of interleukin 1 beta-treated and nontreated keratinocytes were compared using two-dimensional polyacrylamide gel electrophoresis. No significant changes in the molecular pattern of newly synthesized proteins were detected. Finally, none of these lymphokines induced HLA-DR expression by keratinocytes.</description><identifier>ISSN: 0022-202X</identifier><identifier>EISSN: 1523-1747</identifier><identifier>DOI: 10.1111/1523-1747.ep12277378</identifier><identifier>PMID: 2473134</identifier><identifier>CODEN: JIDEAE</identifier><language>eng</language><publisher>Danvers, MA: Elsevier Inc</publisher><subject>Biological and medical sciences ; Cell Division - drug effects ; Cell membranes ; Cells, Cultured ; Dermatology ; Epidermal Cells ; Epidermis - immunology ; Epidermis - metabolism ; Gel electrophoresis ; Histocompatibility antigen HLA ; HLA-DR Antigens - analysis ; HLA-DR Antigens - classification ; Humans ; Interleukin 1 ; Interleukin 2 ; Interleukin-1 - metabolism ; Interleukin-1 - pharmacology ; Interleukin-2 - pharmacology ; Keratinocytes ; Keratins ; Lymphokines ; Medical sciences ; Pharmacology. 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Both interleukin 1 preparations did not stimulate keratinocyte growth; interleukin 2 also did not stimulate keratinocyte growth. To determine whether interleukin 1 beta binds to keratinocytes, a cell membrane assay was developed for these cells. Iodinated interleukin 1 beta binds to keratinocytes with a kD of 6.2nm and 2500 receptors per cell. To determine the effects of interleukin 1 beta on protein synthesis, the molecular patterns of radiolabeled cell extracts of interleukin 1 beta-treated and nontreated keratinocytes were compared using two-dimensional polyacrylamide gel electrophoresis. No significant changes in the molecular pattern of newly synthesized proteins were detected. Finally, none of these lymphokines induced HLA-DR expression by keratinocytes.</description><subject>Biological and medical sciences</subject><subject>Cell Division - drug effects</subject><subject>Cell membranes</subject><subject>Cells, Cultured</subject><subject>Dermatology</subject><subject>Epidermal Cells</subject><subject>Epidermis - immunology</subject><subject>Epidermis - metabolism</subject><subject>Gel electrophoresis</subject><subject>Histocompatibility antigen HLA</subject><subject>HLA-DR Antigens - analysis</subject><subject>HLA-DR Antigens - classification</subject><subject>Humans</subject><subject>Interleukin 1</subject><subject>Interleukin 2</subject><subject>Interleukin-1 - metabolism</subject><subject>Interleukin-1 - pharmacology</subject><subject>Interleukin-2 - pharmacology</subject><subject>Keratinocytes</subject><subject>Keratins</subject><subject>Lymphokines</subject><subject>Medical sciences</subject><subject>Pharmacology. Drug treatments</subject><subject>Protein Biosynthesis</subject><subject>Recombinant Proteins</subject><subject>Skin, nail, hair, dermoskeleton</subject><issn>0022-202X</issn><issn>1523-1747</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUtr3DAURkVoSSaPf5CAN21WTvWyHptCCHmRlEJoITshyVegxJankh3Iv6-HGWbIJtoI7nfuhzhC6JTgCzKfH6ShrCaSywtYEkqlZFLtocV2_AUtMKa0ppg-H6DDUl4wJoI3ah_tUy4ZYXyBfl2HAH4s1RCqJ_BD72Kyaazu0wi5g-k1popUNrUfJrQaUnU39TZVD5DtGNPg30cox-hrsF2Bk819hP7eXP-5uqsff9_eX10-1p5zMtbBS04CKCUV564NjnPRaO8FY44H550ApryWWDsnLcxIoznVWoFglhHBjtD5uneZh38TlNH0sXjoOptgmIrRQhGBFVUz-f1TUmqChRR8Bvka9HkoJUMwyxx7m98NwWbl26zEmpVYs_M9r51t-ifXQ7td2gie82-b3BZvu5Bt8rHsujWTmnG860l2nDJsgUYQxvCq5-c6h1nrW4Rsio-QPLQxz_9n2iF-_tD_LXalPw</recordid><startdate>19890701</startdate><enddate>19890701</enddate><creator>Morhenn, Vera B</creator><creator>Wastek, Gregory J</creator><creator>Cua, Anastasia B</creator><creator>Mansbridge, Jonathan N</creator><general>Elsevier Inc</general><general>Nature Publishing</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19890701</creationdate><title>Effects of Recombinant Interleukin 1 and Interleukin 2 on Human Keratinocytes</title><author>Morhenn, Vera B ; Wastek, Gregory J ; Cua, Anastasia B ; Mansbridge, Jonathan N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c441t-fc741fe887844bdfb44659cc633b4fbcb6e38c9709bb7ae4bd5942998e63a3163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Biological and medical sciences</topic><topic>Cell Division - drug effects</topic><topic>Cell membranes</topic><topic>Cells, Cultured</topic><topic>Dermatology</topic><topic>Epidermal Cells</topic><topic>Epidermis - immunology</topic><topic>Epidermis - metabolism</topic><topic>Gel electrophoresis</topic><topic>Histocompatibility antigen HLA</topic><topic>HLA-DR Antigens - analysis</topic><topic>HLA-DR Antigens - classification</topic><topic>Humans</topic><topic>Interleukin 1</topic><topic>Interleukin 2</topic><topic>Interleukin-1 - metabolism</topic><topic>Interleukin-1 - pharmacology</topic><topic>Interleukin-2 - pharmacology</topic><topic>Keratinocytes</topic><topic>Keratins</topic><topic>Lymphokines</topic><topic>Medical sciences</topic><topic>Pharmacology. Drug treatments</topic><topic>Protein Biosynthesis</topic><topic>Recombinant Proteins</topic><topic>Skin, nail, hair, dermoskeleton</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Morhenn, Vera B</creatorcontrib><creatorcontrib>Wastek, Gregory J</creatorcontrib><creatorcontrib>Cua, Anastasia B</creatorcontrib><creatorcontrib>Mansbridge, Jonathan N</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of investigative dermatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Morhenn, Vera B</au><au>Wastek, Gregory J</au><au>Cua, Anastasia B</au><au>Mansbridge, Jonathan N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Recombinant Interleukin 1 and Interleukin 2 on Human Keratinocytes</atitle><jtitle>Journal of investigative dermatology</jtitle><addtitle>J Invest Dermatol</addtitle><date>1989-07-01</date><risdate>1989</risdate><volume>93</volume><issue>1</issue><spage>121</spage><epage>126</epage><pages>121-126</pages><issn>0022-202X</issn><eissn>1523-1747</eissn><coden>JIDEAE</coden><abstract>The effects of recombinant interleukin 1 alpha and beta, as well as recombinant interleukin 2, on human keratinocyte proliferation were studied in serum-containing as well as defined media. Both interleukin 1 preparations did not stimulate keratinocyte growth; interleukin 2 also did not stimulate keratinocyte growth. To determine whether interleukin 1 beta binds to keratinocytes, a cell membrane assay was developed for these cells. Iodinated interleukin 1 beta binds to keratinocytes with a kD of 6.2nm and 2500 receptors per cell. To determine the effects of interleukin 1 beta on protein synthesis, the molecular patterns of radiolabeled cell extracts of interleukin 1 beta-treated and nontreated keratinocytes were compared using two-dimensional polyacrylamide gel electrophoresis. No significant changes in the molecular pattern of newly synthesized proteins were detected. Finally, none of these lymphokines induced HLA-DR expression by keratinocytes.</abstract><cop>Danvers, MA</cop><pub>Elsevier Inc</pub><pmid>2473134</pmid><doi>10.1111/1523-1747.ep12277378</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of investigative dermatology, 1989-07, Vol.93 (1), p.121-126 |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Biological and medical sciences Cell Division - drug effects Cell membranes Cells, Cultured Dermatology Epidermal Cells Epidermis - immunology Epidermis - metabolism Gel electrophoresis Histocompatibility antigen HLA HLA-DR Antigens - analysis HLA-DR Antigens - classification Humans Interleukin 1 Interleukin 2 Interleukin-1 - metabolism Interleukin-1 - pharmacology Interleukin-2 - pharmacology Keratinocytes Keratins Lymphokines Medical sciences Pharmacology. Drug treatments Protein Biosynthesis Recombinant Proteins Skin, nail, hair, dermoskeleton |
| title | Effects of Recombinant Interleukin 1 and Interleukin 2 on Human Keratinocytes |
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