Cloning and characterization of the 5′-flanking region of the pig AgRP gene

Agouti-related peptide (AgRP), a brain neuropeptide generated by AgRP/neuropeptide Y (NPY) neurons, plays a vital role in the hypothalamic regulation of energy homeostasis. RT-PCR and real-time PCR were carried out in various tissues to detect the AgRP expression pattern in pigs. Our RT-PCR results...

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Veröffentlicht in:	Molecular biology reports 2011-04, Vol.38 (4), p.2233-2239
Hauptverfasser:	Ling, Fei, Wang, Tao, Wei, Liqiong, Zhu, Xiaoping, Chen, Yaosheng, Li, Jiaqi, Zhang, Zongwu, Du, Hongli, Wang, Xiaoning, Wang, Jufang
Format:	Artikel
Sprache:	eng
Schlagworte:	5' Flanking Region - genetics Agouti-Related Protein - genetics Agouti-Related Protein - metabolism Animal Anatomy Animal Biochemistry Animals backfat Bacterial artificial chromosomes Basic Helix-Loop-Helix Transcription Factors - metabolism Biomedical and Life Sciences bladder Brain Chromosomes, Artificial, Bacterial Cloning Cloning, Molecular complementary DNA Differentiation DNA Primers - genetics electrophoresis Electrophoretic Mobility Shift Assay energy Energy balance Energy Metabolism - genetics Gene deletion Gene Expression Profiling genes Heart Histology Hogs Homeostasis hypothalamic regulation Hypothalamus Kidney kidneys landraces Large intestine Life Sciences liver Luciferases Lung Lymph Molecular biology Morphology Nerve Tissue Proteins - metabolism NeuroD protein neurons Neuropeptide Y Oligonucleotides Peptides Polymerase Chain Reaction Promoters quantitative polymerase chain reaction Reverse Transcriptase Polymerase Chain Reaction Species Specificity Spleen stomach swine Swine - genetics Transcription factors Urinary bladder
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