Direct, simultaneous measurement of liposome-encapsulated and released drugs in plasma by on-line SPE–SPE–HPLC
► Liposomal and released drug in plasma were separated by restricted-access media. ► During the extraction, drug release and/or leakage from liposomes was not observed. ► Liposomal and released drug were simultaneously measured by column-switching HPLC. A method for the simultaneous measurement of l...
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| Veröffentlicht in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2011-11, Vol.879 (30), p.3620-3625 |
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| container_title | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
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| creator | Yamamoto, Eiichi Hyodo, Kenji Ohnishi, Naozumi Suzuki, Takuya Ishihara, Hiroshi Kikuchi, Hiroshi Asakawa, Naoki |
| description | ► Liposomal and released drug in plasma were separated by restricted-access media. ► During the extraction, drug release and/or leakage from liposomes was not observed. ► Liposomal and released drug were simultaneously measured by column-switching HPLC.
A method for the simultaneous measurement of liposome-encapsulated and released drugs in mouse plasma by on-line solid phase extraction (SPE)–SPE–HPLC with direct plasma injection was developed using a doxorubicin (DXR)-containing liposome formulation as the model drug. During SPE, the released DXR was extracted on the 1st restricted-access media (RAM) SPE column, whereas the liposomes were eluted. The eluted liposomes were collapsed on-line, and the released DXR was delivered to the 2nd RAM SPE column for extraction. The retained DXR on the SPE columns was analyzed via HPLC–fluorescent detector by switching the valves. The method was validated and applied to the pharmacokinetic study of DXR in mice after intravenous injection of DXR-containing liposomes. |
| doi_str_mv | 10.1016/j.jchromb.2011.10.004 |
| format | Article |
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A method for the simultaneous measurement of liposome-encapsulated and released drugs in mouse plasma by on-line solid phase extraction (SPE)–SPE–HPLC with direct plasma injection was developed using a doxorubicin (DXR)-containing liposome formulation as the model drug. During SPE, the released DXR was extracted on the 1st restricted-access media (RAM) SPE column, whereas the liposomes were eluted. The eluted liposomes were collapsed on-line, and the released DXR was delivered to the 2nd RAM SPE column for extraction. The retained DXR on the SPE columns was analyzed via HPLC–fluorescent detector by switching the valves. The method was validated and applied to the pharmacokinetic study of DXR in mice after intravenous injection of DXR-containing liposomes.</description><identifier>ISSN: 1570-0232</identifier><identifier>EISSN: 1873-376X</identifier><identifier>DOI: 10.1016/j.jchromb.2011.10.004</identifier><identifier>PMID: 22030453</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Acetates ; Analysis ; Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; chromatography ; Chromatography, High Pressure Liquid - methods ; Doxorubicin ; Doxorubicin - administration & dosage ; Doxorubicin - blood ; Doxorubicin - pharmacokinetics ; Drug Stability ; Drugs ; Equipment Design ; Extraction ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; Hydrogen-Ion Concentration ; intravenous injection ; Liposomes ; Liposomes - blood ; Liposomes - pharmacokinetics ; Male ; Medical sciences ; Methanol ; Mice ; Mice, Inbred BALB C ; On-line systems ; pharmacokinetics ; Pharmacology. Drug treatments ; Plasma ; Random access memory ; Reproducibility of Results ; Restricted-access media ; Solid Phase Extraction - methods ; Solid phases ; Solid-phase extraction ; Switching ; Valves</subject><ispartof>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2011-11, Vol.879 (30), p.3620-3625</ispartof><rights>2011 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c548t-b3fdcc14b9bfe65c8badf3db45624ec790a579d5e53d2c7b08ed9ba1c46e1e033</citedby><cites>FETCH-LOGICAL-c548t-b3fdcc14b9bfe65c8badf3db45624ec790a579d5e53d2c7b08ed9ba1c46e1e033</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jchromb.2011.10.004$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24749994$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22030453$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yamamoto, Eiichi</creatorcontrib><creatorcontrib>Hyodo, Kenji</creatorcontrib><creatorcontrib>Ohnishi, Naozumi</creatorcontrib><creatorcontrib>Suzuki, Takuya</creatorcontrib><creatorcontrib>Ishihara, Hiroshi</creatorcontrib><creatorcontrib>Kikuchi, Hiroshi</creatorcontrib><creatorcontrib>Asakawa, Naoki</creatorcontrib><title>Direct, simultaneous measurement of liposome-encapsulated and released drugs in plasma by on-line SPE–SPE–HPLC</title><title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><description>► Liposomal and released drug in plasma were separated by restricted-access media. ► During the extraction, drug release and/or leakage from liposomes was not observed. ► Liposomal and released drug were simultaneously measured by column-switching HPLC.
A method for the simultaneous measurement of liposome-encapsulated and released drugs in mouse plasma by on-line solid phase extraction (SPE)–SPE–HPLC with direct plasma injection was developed using a doxorubicin (DXR)-containing liposome formulation as the model drug. During SPE, the released DXR was extracted on the 1st restricted-access media (RAM) SPE column, whereas the liposomes were eluted. The eluted liposomes were collapsed on-line, and the released DXR was delivered to the 2nd RAM SPE column for extraction. The retained DXR on the SPE columns was analyzed via HPLC–fluorescent detector by switching the valves. The method was validated and applied to the pharmacokinetic study of DXR in mice after intravenous injection of DXR-containing liposomes.</description><subject>Acetates</subject><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>chromatography</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Doxorubicin</subject><subject>Doxorubicin - administration & dosage</subject><subject>Doxorubicin - blood</subject><subject>Doxorubicin - pharmacokinetics</subject><subject>Drug Stability</subject><subject>Drugs</subject><subject>Equipment Design</subject><subject>Extraction</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>Hydrogen-Ion Concentration</subject><subject>intravenous injection</subject><subject>Liposomes</subject><subject>Liposomes - blood</subject><subject>Liposomes - pharmacokinetics</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Methanol</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>On-line systems</subject><subject>pharmacokinetics</subject><subject>Pharmacology. Drug treatments</subject><subject>Plasma</subject><subject>Random access memory</subject><subject>Reproducibility of Results</subject><subject>Restricted-access media</subject><subject>Solid Phase Extraction - methods</subject><subject>Solid phases</subject><subject>Solid-phase extraction</subject><subject>Switching</subject><subject>Valves</subject><issn>1570-0232</issn><issn>1873-376X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNks1u1DAQgCMEoqXwCIAviAtZxvFfckJoWyjSSlQqlbhZjj0pXuWvdoLUW9-BN-RJcMgCx3KxPaNvZmx9zrLnFDYUqHy73-zttzB09aYASlNuA8AfZMe0VCxnSn59mM5CQQ4FK46yJzHuAagCxR5nR0UBDLhgx1k49QHt9IZE383tZHoc5kg6NHEO2GE_kaEhrR-HOHSYY2_NGOfWTOiI6R0J2CY0BS7M15H4noytiZ0h9S0Z-rz1PZLLi7Ofdz_W9fxit32aPWpMG_HZYT_Jrj6cfdme57vPHz9t3-9yK3g55TVrnLWU11XdoBS2rI1rmKu5kAVHqyowQlVOoGCusKqGEl1VG2q5RIrA2En2eu07huFmxjjpzkeLbbs-UleSlZXggv4XqVQl4X4SCqmKUi7TxUraMMQYsNFj8J0Jt5qCXgzqvT4Y1IvBJZ0MproXhwlz3aH7W_VHWQJeHQATrWmbYHrr4z-OK15V1dLo5co1ZtDmOiTm6jJNkgAgFeUqEe9WApOF7x6DjtYnxeh-_wntBn_PZX8BFizIJQ</recordid><startdate>20111115</startdate><enddate>20111115</enddate><creator>Yamamoto, Eiichi</creator><creator>Hyodo, Kenji</creator><creator>Ohnishi, Naozumi</creator><creator>Suzuki, Takuya</creator><creator>Ishihara, Hiroshi</creator><creator>Kikuchi, Hiroshi</creator><creator>Asakawa, Naoki</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TB</scope><scope>8FD</scope><scope>FR3</scope></search><sort><creationdate>20111115</creationdate><title>Direct, simultaneous measurement of liposome-encapsulated and released drugs in plasma by on-line SPE–SPE–HPLC</title><author>Yamamoto, Eiichi ; Hyodo, Kenji ; Ohnishi, Naozumi ; Suzuki, Takuya ; Ishihara, Hiroshi ; Kikuchi, Hiroshi ; Asakawa, Naoki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c548t-b3fdcc14b9bfe65c8badf3db45624ec790a579d5e53d2c7b08ed9ba1c46e1e033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Acetates</topic><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>chromatography</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Doxorubicin</topic><topic>Doxorubicin - administration & dosage</topic><topic>Doxorubicin - blood</topic><topic>Doxorubicin - pharmacokinetics</topic><topic>Drug Stability</topic><topic>Drugs</topic><topic>Equipment Design</topic><topic>Extraction</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>Hydrogen-Ion Concentration</topic><topic>intravenous injection</topic><topic>Liposomes</topic><topic>Liposomes - blood</topic><topic>Liposomes - pharmacokinetics</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Methanol</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>On-line systems</topic><topic>pharmacokinetics</topic><topic>Pharmacology. 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B, Analytical technologies in the biomedical and life sciences</jtitle><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><date>2011-11-15</date><risdate>2011</risdate><volume>879</volume><issue>30</issue><spage>3620</spage><epage>3625</epage><pages>3620-3625</pages><issn>1570-0232</issn><eissn>1873-376X</eissn><abstract>► Liposomal and released drug in plasma were separated by restricted-access media. ► During the extraction, drug release and/or leakage from liposomes was not observed. ► Liposomal and released drug were simultaneously measured by column-switching HPLC.
A method for the simultaneous measurement of liposome-encapsulated and released drugs in mouse plasma by on-line solid phase extraction (SPE)–SPE–HPLC with direct plasma injection was developed using a doxorubicin (DXR)-containing liposome formulation as the model drug. During SPE, the released DXR was extracted on the 1st restricted-access media (RAM) SPE column, whereas the liposomes were eluted. The eluted liposomes were collapsed on-line, and the released DXR was delivered to the 2nd RAM SPE column for extraction. The retained DXR on the SPE columns was analyzed via HPLC–fluorescent detector by switching the valves. The method was validated and applied to the pharmacokinetic study of DXR in mice after intravenous injection of DXR-containing liposomes.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>22030453</pmid><doi>10.1016/j.jchromb.2011.10.004</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 1570-0232 |
| ispartof | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2011-11, Vol.879 (30), p.3620-3625 |
| issn | 1570-0232 1873-376X |
| language | eng |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Acetates Analysis Analytical, structural and metabolic biochemistry Animals Biological and medical sciences chromatography Chromatography, High Pressure Liquid - methods Doxorubicin Doxorubicin - administration & dosage Doxorubicin - blood Doxorubicin - pharmacokinetics Drug Stability Drugs Equipment Design Extraction Fundamental and applied biological sciences. Psychology General pharmacology Hydrogen-Ion Concentration intravenous injection Liposomes Liposomes - blood Liposomes - pharmacokinetics Male Medical sciences Methanol Mice Mice, Inbred BALB C On-line systems pharmacokinetics Pharmacology. Drug treatments Plasma Random access memory Reproducibility of Results Restricted-access media Solid Phase Extraction - methods Solid phases Solid-phase extraction Switching Valves |
| title | Direct, simultaneous measurement of liposome-encapsulated and released drugs in plasma by on-line SPE–SPE–HPLC |
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