Microfluidic chemostat and turbidostat with flow rate, oxygen, and temperature control for dynamic continuous culture

This work reports on an instrument capable of supporting automated microscale continuous culture experiments. The instrument consists of a plastic-PDMS device capable of continuous flow without volume drift or evaporation. We apply direct computer controlled machining and chemical bonding fabricatio...

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Veröffentlicht in:	Lab on a chip 2011-01, Vol.11 (10), p.1730-1739
Hauptverfasser:	Lee, Kevin S, Boccazzi, Paolo, Sinskey, Anthony J, Ram, Rajeev J
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell Culture Techniques - instrumentation Cell Culture Techniques - methods Culture Density Devices Dimethylpolysiloxanes - chemistry Dissolution Dynamical systems Dynamics Hydrogen-Ion Concentration Microfluidic Analytical Techniques - instrumentation Microfluidic Analytical Techniques - methods Microfluidics Oxygen - chemistry Temperature Temperature control
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