Site specific chemoselective labelling of proteins with robust and highly sensitive Ru(II) bathophenanthroline complexes
The bioorthogonal and chemoselective fluorescence labelling of several cell-free synthesized proteins containing a site-specifically incorporated azido amino acid was possible using different alkyne-functionalized Ru(II) bathophenanthroline complexes. We were able to achieve a selective labelling ev...
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| Veröffentlicht in: | Organic & biomolecular chemistry 2012-03, Vol.10 (11), p.2223-2226 |
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| container_title | Organic & biomolecular chemistry |
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| creator | Uzagare, Matthew C Claussnitzer, Iris Gerrits, Michael Bannwarth, Willi |
| description | The bioorthogonal and chemoselective fluorescence labelling of several cell-free synthesized proteins containing a site-specifically incorporated azido amino acid was possible using different alkyne-functionalized Ru(II) bathophenanthroline complexes. We were able to achieve a selective labelling even in complex mixtures of proteins despite the fact that ruthenium dyes normally show a high tendency for unspecific interactions with proteins and are commonly used for total staining of proteins. Since the employed Ru complexes are extremely robust, photo-stable and highly sensitive, the approach should be applicable to the production of labelled proteins for single molecule spectroscopy and fluorescence-based interaction studies. |
| doi_str_mv | 10.1039/c2ob06684j |
| format | Article |
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Since the employed Ru complexes are extremely robust, photo-stable and highly sensitive, the approach should be applicable to the production of labelled proteins for single molecule spectroscopy and fluorescence-based interaction studies.</description><subject>Molecular Structure</subject><subject>Phenanthrolines - chemistry</subject><subject>Proteins - analysis</subject><subject>Proteins - chemistry</subject><subject>Ruthenium - chemistry</subject><issn>1477-0520</issn><issn>1477-0539</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkMtOwzAQRS0EolDY8AHIOx5SwY4TO16iikelSkg81pHtTBpXSRxiB9q_J9BSVnMXZ45mLkJnlNxQwuStiZwmnKfxcg8d0ViICUmY3N_liIzQsfdLQqgUPD5EoyhijNKYHqHVqw2AfQvGFtZgU0LtPFRggv0EXCkNVWWbBXYFbjsXwDYef9lQ4s7p3gesmhyXdlFWa-yh8fZ37aW_nM2usFahdG0JjWpC2bnBA9i4uq1gBf4EHRSq8nC6nWP0_nD_Nn2azJ8fZ9O7-cREKQ8TVXCaSCJ5AokRXOkhM5BSU2F4LrSRWkaMCENpWkSaS5FIFgkuE5HkhZZsjC423uH8jx58yGrrzfCVasD1PpOcpYwJng7k9YY0nfO-gyJrO1urbp1Rkv0Unf0XPcDnW22va8h36F-z7BsvuHri</recordid><startdate>20120321</startdate><enddate>20120321</enddate><creator>Uzagare, Matthew C</creator><creator>Claussnitzer, Iris</creator><creator>Gerrits, Michael</creator><creator>Bannwarth, Willi</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120321</creationdate><title>Site specific chemoselective labelling of proteins with robust and highly sensitive Ru(II) bathophenanthroline complexes</title><author>Uzagare, Matthew C ; Claussnitzer, Iris ; Gerrits, Michael ; Bannwarth, Willi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c286t-af61590965e5c76ab9093e99b17c6d7bc9b92307c118f2b6975932769575dfb93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Molecular Structure</topic><topic>Phenanthrolines - chemistry</topic><topic>Proteins - analysis</topic><topic>Proteins - chemistry</topic><topic>Ruthenium - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Uzagare, Matthew C</creatorcontrib><creatorcontrib>Claussnitzer, Iris</creatorcontrib><creatorcontrib>Gerrits, Michael</creatorcontrib><creatorcontrib>Bannwarth, Willi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Organic & biomolecular chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Uzagare, Matthew C</au><au>Claussnitzer, Iris</au><au>Gerrits, Michael</au><au>Bannwarth, Willi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Site specific chemoselective labelling of proteins with robust and highly sensitive Ru(II) bathophenanthroline complexes</atitle><jtitle>Organic & biomolecular chemistry</jtitle><addtitle>Org Biomol Chem</addtitle><date>2012-03-21</date><risdate>2012</risdate><volume>10</volume><issue>11</issue><spage>2223</spage><epage>2226</epage><pages>2223-2226</pages><issn>1477-0520</issn><eissn>1477-0539</eissn><abstract>The bioorthogonal and chemoselective fluorescence labelling of several cell-free synthesized proteins containing a site-specifically incorporated azido amino acid was possible using different alkyne-functionalized Ru(II) bathophenanthroline complexes. 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| ispartof | Organic & biomolecular chemistry, 2012-03, Vol.10 (11), p.2223-2226 |
| issn | 1477-0520 1477-0539 |
| language | eng |
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| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Molecular Structure Phenanthrolines - chemistry Proteins - analysis Proteins - chemistry Ruthenium - chemistry |
| title | Site specific chemoselective labelling of proteins with robust and highly sensitive Ru(II) bathophenanthroline complexes |
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