Histamine may induce airway remodeling through release of epidermal growth factor receptor ligands from bronchial epithelial cells
Asthma is a chronic inflammatory disease that is associated with airway remodeling, including hyperplasia of airway epithelial cells and airway smooth muscle cells, and goblet cell differentiation. We wished to address the potential role of histamine, a key biogenic amine involved in allergic reacti...
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| Veröffentlicht in: | The FASEB journal 2012-04, Vol.26 (4), p.1704-1716 |
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| creator | Hirota, Nobuaki Risse, Paul‐Andre Novali, Mauro McGovern, Toby Al‐Alwan, Laila McCuaig, Sarah Proud, David Hayden, Patrick Hamid, Qutayba Martin, James G. |
| description | Asthma is a chronic inflammatory disease that is associated with airway remodeling, including hyperplasia of airway epithelial cells and airway smooth muscle cells, and goblet cell differentiation. We wished to address the potential role of histamine, a key biogenic amine involved in allergic reactions, in airway remodeling through the epidermal growth factor receptor (EGFR) pathway. Here, we demonstrate that histamine releases 2 EGFR ligands, amphiregulin and heparin‐binding epidermal growth factor‐like growth factor (HB‐EGF), from airway epithelial cells. Amphiregulin and HB‐EGF were expressed in airway epithelium of patients with asthma. Histamine up‐regulated their mRNA expression (amphiregulin 3.2‐fold, P< 0.001; HB‐EGF 2.3‐fold, P |
| doi_str_mv | 10.1096/fj.11-197061 |
| format | Article |
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We wished to address the potential role of histamine, a key biogenic amine involved in allergic reactions, in airway remodeling through the epidermal growth factor receptor (EGFR) pathway. Here, we demonstrate that histamine releases 2 EGFR ligands, amphiregulin and heparin‐binding epidermal growth factor‐like growth factor (HB‐EGF), from airway epithelial cells. Amphiregulin and HB‐EGF were expressed in airway epithelium of patients with asthma. Histamine up‐regulated their mRNA expression (amphiregulin 3.2‐fold, P< 0.001; HB‐EGF 2.3‐fold, P<0.05) and triggered their release (amphiregulin EC50 0.50 μM, 31.2±2.7 pg/ml with 10 μM histamine, P<0.01; HB‐EGF EC50 0.54 μM, 78.5±1.8 pg/ml with 10 μM histamine, P<0.001) compared to vehicle control (amphiregulin 19.3±0.9 pg/ ml; HB‐EGF 60.2± 1.0 pg/ml), in airway epithelial cells. Histamine increased EGFR phosphorylation (2.1‐fold by Western blot analysis) and induced goblet cell differentiation (CLCA1 up‐regulation by real‐time qPCR) in normal human bronchial epithelial (NHBE) cells. Moreover, amphiregulin and HB‐EGF caused proliferation and migration of both NHBE cells and human airway smooth muscle cells. These results suggest that histamine may induce airway remodeling via the epithelial‐derived EGFR ligands amphiregulin and HB‐EGF.—Hirota, N., Risse, P.‐A., Novali, M., McGovern, T., Al‐Alwan, L., McCuaig, S., Proud, D., Hayden, P., Hamid, Q., Martin, J. G. Histamine may induce airway remodeling through release of epidermal growth factor receptor ligands from bronchial epithelial cells. FASEB J. 26, 1704‐1716 (2012). www.fasebj.org</description><identifier>ISSN: 0892-6638</identifier><identifier>EISSN: 1530-6860</identifier><identifier>DOI: 10.1096/fj.11-197061</identifier><identifier>PMID: 22247333</identifier><language>eng</language><publisher>Bethesda, MD, USA: Federation of American Societies for Experimental Biology</publisher><subject>Adult ; Airway Remodeling - drug effects ; Amphiregulin ; Asthma - metabolism ; Asthma - pathology ; Bronchi - cytology ; Cell Differentiation - drug effects ; Cell Line ; Cell Movement ; Cell Proliferation ; EGF Family of Proteins ; Epithelial Cells - cytology ; Epithelial Cells - drug effects ; Epithelial Cells - metabolism ; Glycoproteins - genetics ; Glycoproteins - metabolism ; goblet cell differentiation ; HB‐EGF ; Heparin-binding EGF-like Growth Factor ; heparin‐binding epidermal growth factor‐like growth factor ; Histamine - pharmacology ; Humans ; Intercellular Signaling Peptides and Proteins - genetics ; Intercellular Signaling Peptides and Proteins - metabolism ; Ligands ; Middle Aged ; migration ; proliferation ; Receptor, Epidermal Growth Factor - metabolism ; Receptors, Histamine H1 - metabolism ; Young Adult</subject><ispartof>The FASEB journal, 2012-04, Vol.26 (4), p.1704-1716</ispartof><rights>FASEB</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4043-c5fa19f9b0f008c71ec650e34e34c6aa75e78f01287bb82ac0b54a4cd5ae3fd33</citedby><cites>FETCH-LOGICAL-c4043-c5fa19f9b0f008c71ec650e34e34c6aa75e78f01287bb82ac0b54a4cd5ae3fd33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1096%2Ffj.11-197061$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1096%2Ffj.11-197061$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22247333$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hirota, Nobuaki</creatorcontrib><creatorcontrib>Risse, Paul‐Andre</creatorcontrib><creatorcontrib>Novali, Mauro</creatorcontrib><creatorcontrib>McGovern, Toby</creatorcontrib><creatorcontrib>Al‐Alwan, Laila</creatorcontrib><creatorcontrib>McCuaig, Sarah</creatorcontrib><creatorcontrib>Proud, David</creatorcontrib><creatorcontrib>Hayden, Patrick</creatorcontrib><creatorcontrib>Hamid, Qutayba</creatorcontrib><creatorcontrib>Martin, James G.</creatorcontrib><title>Histamine may induce airway remodeling through release of epidermal growth factor receptor ligands from bronchial epithelial cells</title><title>The FASEB journal</title><addtitle>FASEB J</addtitle><description>Asthma is a chronic inflammatory disease that is associated with airway remodeling, including hyperplasia of airway epithelial cells and airway smooth muscle cells, and goblet cell differentiation. We wished to address the potential role of histamine, a key biogenic amine involved in allergic reactions, in airway remodeling through the epidermal growth factor receptor (EGFR) pathway. Here, we demonstrate that histamine releases 2 EGFR ligands, amphiregulin and heparin‐binding epidermal growth factor‐like growth factor (HB‐EGF), from airway epithelial cells. Amphiregulin and HB‐EGF were expressed in airway epithelium of patients with asthma. Histamine up‐regulated their mRNA expression (amphiregulin 3.2‐fold, P< 0.001; HB‐EGF 2.3‐fold, P<0.05) and triggered their release (amphiregulin EC50 0.50 μM, 31.2±2.7 pg/ml with 10 μM histamine, P<0.01; HB‐EGF EC50 0.54 μM, 78.5±1.8 pg/ml with 10 μM histamine, P<0.001) compared to vehicle control (amphiregulin 19.3±0.9 pg/ ml; HB‐EGF 60.2± 1.0 pg/ml), in airway epithelial cells. Histamine increased EGFR phosphorylation (2.1‐fold by Western blot analysis) and induced goblet cell differentiation (CLCA1 up‐regulation by real‐time qPCR) in normal human bronchial epithelial (NHBE) cells. Moreover, amphiregulin and HB‐EGF caused proliferation and migration of both NHBE cells and human airway smooth muscle cells. These results suggest that histamine may induce airway remodeling via the epithelial‐derived EGFR ligands amphiregulin and HB‐EGF.—Hirota, N., Risse, P.‐A., Novali, M., McGovern, T., Al‐Alwan, L., McCuaig, S., Proud, D., Hayden, P., Hamid, Q., Martin, J. G. Histamine may induce airway remodeling through release of epidermal growth factor receptor ligands from bronchial epithelial cells. FASEB J. 26, 1704‐1716 (2012). www.fasebj.org</description><subject>Adult</subject><subject>Airway Remodeling - drug effects</subject><subject>Amphiregulin</subject><subject>Asthma - metabolism</subject><subject>Asthma - pathology</subject><subject>Bronchi - cytology</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Line</subject><subject>Cell Movement</subject><subject>Cell Proliferation</subject><subject>EGF Family of Proteins</subject><subject>Epithelial Cells - cytology</subject><subject>Epithelial Cells - drug effects</subject><subject>Epithelial Cells - metabolism</subject><subject>Glycoproteins - genetics</subject><subject>Glycoproteins - metabolism</subject><subject>goblet cell differentiation</subject><subject>HB‐EGF</subject><subject>Heparin-binding EGF-like Growth Factor</subject><subject>heparin‐binding epidermal growth factor‐like growth factor</subject><subject>Histamine - pharmacology</subject><subject>Humans</subject><subject>Intercellular Signaling Peptides and Proteins - genetics</subject><subject>Intercellular Signaling Peptides and Proteins - metabolism</subject><subject>Ligands</subject><subject>Middle Aged</subject><subject>migration</subject><subject>proliferation</subject><subject>Receptor, Epidermal Growth Factor - metabolism</subject><subject>Receptors, Histamine H1 - metabolism</subject><subject>Young Adult</subject><issn>0892-6638</issn><issn>1530-6860</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1r3DAQxUVoSbZJbjkH3XqJk5Fky_axDdmkEMihyVnI8mitxba2ks2y1_7l1eJtj4WB-eD3HsMj5IbBPYNaPtjtPWMZq0uQ7IysWCEgk5WET2QFVc0zKUV1Qb7EuAUABkyekwvOeV4KIVbk94uLkx7ciHTQB-rGdjZItQv7tAUcfIu9Gzd06oKfN1069agjUm8p7lyLYdA93QS_nzpqtZl8SIjB3XHo3UaPbaQ2-IE2wY-mc4lOuqlLrmk02Pfxiny2uo94feqX5GP99P74kr2-Pf94_PaamRxykZnCalbbugELUJmSoZEFoMhTGal1WWBZWWC8Kpum4tpAU-Q6N22hUdhWiEvydfHdBf9rxjipwcXjB3pEP0dVp6AElIwn8m4hTfAxBrRqF9ygw0ExUMfQld0qxtQSesJvT8ZzM2D7D_6bcgLKBdi7Hg__NVPrn985cAmQQ1L-AZqxkHQ</recordid><startdate>201204</startdate><enddate>201204</enddate><creator>Hirota, Nobuaki</creator><creator>Risse, Paul‐Andre</creator><creator>Novali, Mauro</creator><creator>McGovern, Toby</creator><creator>Al‐Alwan, Laila</creator><creator>McCuaig, Sarah</creator><creator>Proud, David</creator><creator>Hayden, Patrick</creator><creator>Hamid, Qutayba</creator><creator>Martin, James G.</creator><general>Federation of American Societies for Experimental Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201204</creationdate><title>Histamine may induce airway remodeling through release of epidermal growth factor receptor ligands from bronchial epithelial cells</title><author>Hirota, Nobuaki ; Risse, Paul‐Andre ; Novali, Mauro ; McGovern, Toby ; Al‐Alwan, Laila ; McCuaig, Sarah ; Proud, David ; Hayden, Patrick ; Hamid, Qutayba ; Martin, James G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4043-c5fa19f9b0f008c71ec650e34e34c6aa75e78f01287bb82ac0b54a4cd5ae3fd33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adult</topic><topic>Airway Remodeling - drug effects</topic><topic>Amphiregulin</topic><topic>Asthma - metabolism</topic><topic>Asthma - pathology</topic><topic>Bronchi - cytology</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Line</topic><topic>Cell Movement</topic><topic>Cell Proliferation</topic><topic>EGF Family of Proteins</topic><topic>Epithelial Cells - cytology</topic><topic>Epithelial Cells - drug effects</topic><topic>Epithelial Cells - metabolism</topic><topic>Glycoproteins - genetics</topic><topic>Glycoproteins - metabolism</topic><topic>goblet cell differentiation</topic><topic>HB‐EGF</topic><topic>Heparin-binding EGF-like Growth Factor</topic><topic>heparin‐binding epidermal growth factor‐like growth factor</topic><topic>Histamine - pharmacology</topic><topic>Humans</topic><topic>Intercellular Signaling Peptides and Proteins - genetics</topic><topic>Intercellular Signaling Peptides and Proteins - metabolism</topic><topic>Ligands</topic><topic>Middle Aged</topic><topic>migration</topic><topic>proliferation</topic><topic>Receptor, Epidermal Growth Factor - metabolism</topic><topic>Receptors, Histamine H1 - metabolism</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hirota, Nobuaki</creatorcontrib><creatorcontrib>Risse, Paul‐Andre</creatorcontrib><creatorcontrib>Novali, Mauro</creatorcontrib><creatorcontrib>McGovern, Toby</creatorcontrib><creatorcontrib>Al‐Alwan, Laila</creatorcontrib><creatorcontrib>McCuaig, Sarah</creatorcontrib><creatorcontrib>Proud, David</creatorcontrib><creatorcontrib>Hayden, Patrick</creatorcontrib><creatorcontrib>Hamid, Qutayba</creatorcontrib><creatorcontrib>Martin, James G.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The FASEB journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hirota, Nobuaki</au><au>Risse, Paul‐Andre</au><au>Novali, Mauro</au><au>McGovern, Toby</au><au>Al‐Alwan, Laila</au><au>McCuaig, Sarah</au><au>Proud, David</au><au>Hayden, Patrick</au><au>Hamid, Qutayba</au><au>Martin, James G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Histamine may induce airway remodeling through release of epidermal growth factor receptor ligands from bronchial epithelial cells</atitle><jtitle>The FASEB journal</jtitle><addtitle>FASEB J</addtitle><date>2012-04</date><risdate>2012</risdate><volume>26</volume><issue>4</issue><spage>1704</spage><epage>1716</epage><pages>1704-1716</pages><issn>0892-6638</issn><eissn>1530-6860</eissn><abstract>Asthma is a chronic inflammatory disease that is associated with airway remodeling, including hyperplasia of airway epithelial cells and airway smooth muscle cells, and goblet cell differentiation. We wished to address the potential role of histamine, a key biogenic amine involved in allergic reactions, in airway remodeling through the epidermal growth factor receptor (EGFR) pathway. Here, we demonstrate that histamine releases 2 EGFR ligands, amphiregulin and heparin‐binding epidermal growth factor‐like growth factor (HB‐EGF), from airway epithelial cells. Amphiregulin and HB‐EGF were expressed in airway epithelium of patients with asthma. Histamine up‐regulated their mRNA expression (amphiregulin 3.2‐fold, P< 0.001; HB‐EGF 2.3‐fold, P<0.05) and triggered their release (amphiregulin EC50 0.50 μM, 31.2±2.7 pg/ml with 10 μM histamine, P<0.01; HB‐EGF EC50 0.54 μM, 78.5±1.8 pg/ml with 10 μM histamine, P<0.001) compared to vehicle control (amphiregulin 19.3±0.9 pg/ ml; HB‐EGF 60.2± 1.0 pg/ml), in airway epithelial cells. Histamine increased EGFR phosphorylation (2.1‐fold by Western blot analysis) and induced goblet cell differentiation (CLCA1 up‐regulation by real‐time qPCR) in normal human bronchial epithelial (NHBE) cells. Moreover, amphiregulin and HB‐EGF caused proliferation and migration of both NHBE cells and human airway smooth muscle cells. These results suggest that histamine may induce airway remodeling via the epithelial‐derived EGFR ligands amphiregulin and HB‐EGF.—Hirota, N., Risse, P.‐A., Novali, M., McGovern, T., Al‐Alwan, L., McCuaig, S., Proud, D., Hayden, P., Hamid, Q., Martin, J. G. Histamine may induce airway remodeling through release of epidermal growth factor receptor ligands from bronchial epithelial cells. FASEB J. 26, 1704‐1716 (2012). www.fasebj.org</abstract><cop>Bethesda, MD, USA</cop><pub>Federation of American Societies for Experimental Biology</pub><pmid>22247333</pmid><doi>10.1096/fj.11-197061</doi><tpages>13</tpages></addata></record> |
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| subjects | Adult Airway Remodeling - drug effects Amphiregulin Asthma - metabolism Asthma - pathology Bronchi - cytology Cell Differentiation - drug effects Cell Line Cell Movement Cell Proliferation EGF Family of Proteins Epithelial Cells - cytology Epithelial Cells - drug effects Epithelial Cells - metabolism Glycoproteins - genetics Glycoproteins - metabolism goblet cell differentiation HB‐EGF Heparin-binding EGF-like Growth Factor heparin‐binding epidermal growth factor‐like growth factor Histamine - pharmacology Humans Intercellular Signaling Peptides and Proteins - genetics Intercellular Signaling Peptides and Proteins - metabolism Ligands Middle Aged migration proliferation Receptor, Epidermal Growth Factor - metabolism Receptors, Histamine H1 - metabolism Young Adult |
| title | Histamine may induce airway remodeling through release of epidermal growth factor receptor ligands from bronchial epithelial cells |
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