Vector-initiated transitive RNA interference in the filamentous fungus Aspergillus oryzae

► Small gene fragments inserted upstream of a vector transcribed inverted repeat undergo silencing. ► Spore color screens demonstrate efficiency of transitive RNAi comparable to that of hairpin induced RNAi. ► Over expression of the endogenous RdRP increases the incidence of transitive RNAi nearly t...

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Veröffentlicht in:	Fungal genetics and biology 2012-04, Vol.49 (4), p.294-301
Hauptverfasser:	Fernandez, Evee Q., Moyer, Donna L., Maiyuran, Suchindra, Labaro, Anna, Brody, Howard
Format:	Artikel
Sprache:	eng
Schlagworte:	Arabidopsis thaliana Aspergillus oryzae Aspergillus oryzae - genetics Aspergillus oryzae - metabolism Caenorhabditis elegans color DNA-directed RNA polymerase Double-stranded RNA Escherichia coli Escherichia coli - genetics Fungal Proteins - genetics fungi gene expression Gene Expression Regulation, Fungal - genetics Gene Silencing genes Genetic Vectors Inverted Repeat Sequences messenger RNA Phosphotransferases (Alcohol Group Acceptor) - genetics Plasmids - genetics Polyketide Synthases - genetics Promoter Regions, Genetic - genetics RNA dependent RNA Polymerase RNA Interference RNA Replicase - genetics RNA, Double-Stranded - genetics RNA, Fungal - genetics RNA, Messenger - genetics small interfering RNA spores Spores, Fungal Transitive RNAi
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