Rapid detection of rRNA group I pseudomonads in contaminated metalworking fluids and biofilm formation by fluorescent in situ hybridization

Metalworking fluids (MWFs), used in different machining operations, are highly prone to microbial degradation. Microbial communities present in MWFs lead to biofilm formation in the MWF systems, which act as a continuous source of contamination. Species of rRNA group I Pseudomonas dominate in contam...

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Veröffentlicht in:	Applied microbiology and biotechnology 2012-05, Vol.94 (3), p.799-808
Hauptverfasser:	Saha, Ratul, Donofrio, Robert S., Goeres, Darla M., Bagley, Susan T.
Format:	Artikel
Sprache:	eng
Schlagworte:	Abundance Analysis Bacteria Bacterial Load Biofilms Biofilms - growth & development Biological products Biomedical and Life Sciences Biotechnology Contamination Culture techniques Cytogenetics DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics E coli Environmental Biotechnology Environmental Microbiology High speed machining Hybridization In Situ Hybridization, Fluorescence - methods Life Sciences Lubricants & lubrication Metal products industry Metalworking fluids Metalworking industry Metalworking machinery Microbial activity Microbial degradation Microbial Genetics and Genomics Microbiology Microorganisms Monitors Pathogens Pseudomonas Pseudomonas fluorescens Pseudomonas fluorescens - genetics Pseudomonas fluorescens - isolation & purification Pseudomonas fluorescens - physiology Pseudomonas oleovorans Pseudomonas oleovorans - genetics Pseudomonas oleovorans - isolation & purification Pseudomonas oleovorans - physiology RNA RNA, Ribosomal - genetics RNA, Ribosomal, 16S - genetics Sensitivity and Specificity Studies
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