Human papillomavirus detection in dysplastic and malignant oral verrucous lesions
The role of human papillomaviruses (HPV) in dysplastic and malignant oral verrucous lesions is controversial since there is a wide range in the incidence of virus detection. This study used a multi-tiered method of HPV detection using DNA in-situ hybridisation (ISH) for low- and high-risk subtypes,...
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| Veröffentlicht in: | Journal of clinical pathology 2012-03, Vol.65 (3), p.283-286 |
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| container_title | Journal of clinical pathology |
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| creator | Stokes, Angela Guerra, Eliete Bible, Jon Halligan, Eugene Orchard, Guy Odell, Edward Thavaraj, Selvam |
| description | The role of human papillomaviruses (HPV) in dysplastic and malignant oral verrucous lesions is controversial since there is a wide range in the incidence of virus detection. This study used a multi-tiered method of HPV detection using DNA in-situ hybridisation (ISH) for low- and high-risk subtypes, consensus PCR, and HPV genotype analysis in archival tissue from 20 cases of dysplastic and malignant oral verrucous lesions. The biological significance of HPV DNA detection was assessed by p16 immunohistochemistry (IHC). While 1/7 carcinomas and 5/13 dysplasias contained HPV DNA by consensus PCR and genotype analysis, all specimens were negative for low- and high-risk HPV ISH and negative for p16 IHC. Results show that although high-risk HPV DNA is detectable in a subset of these lesions, the lack of p16 overexpression suggests that the oncogenic process is not driven by HPV oncoproteins. |
| doi_str_mv | 10.1136/jclinpath-2011-200454 |
| format | Article |
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This study used a multi-tiered method of HPV detection using DNA in-situ hybridisation (ISH) for low- and high-risk subtypes, consensus PCR, and HPV genotype analysis in archival tissue from 20 cases of dysplastic and malignant oral verrucous lesions. The biological significance of HPV DNA detection was assessed by p16 immunohistochemistry (IHC). While 1/7 carcinomas and 5/13 dysplasias contained HPV DNA by consensus PCR and genotype analysis, all specimens were negative for low- and high-risk HPV ISH and negative for p16 IHC. Results show that although high-risk HPV DNA is detectable in a subset of these lesions, the lack of p16 overexpression suggests that the oncogenic process is not driven by HPV oncoproteins.</description><identifier>ISSN: 0021-9746</identifier><identifier>EISSN: 1472-4146</identifier><identifier>DOI: 10.1136/jclinpath-2011-200454</identifier><identifier>PMID: 22174425</identifier><identifier>CODEN: JCPAAK</identifier><language>eng</language><publisher>England: BMJ Publishing Group Ltd and Association of Clinical Pathologists</publisher><subject>Aged ; Aged, 80 and over ; Biomarkers, Tumor - analysis ; cancer ; cancer research ; Carcinoma, Verrucous - chemistry ; Carcinoma, Verrucous - pathology ; Carcinoma, Verrucous - virology ; Cervical cancer ; Cyclin-Dependent Kinase Inhibitor p16 - analysis ; Deoxyribonucleic acid ; DNA ; DNA, Viral - isolation & purification ; dysplasia ; Female ; Genotype ; head and neck ; head and neck cancer ; HPV ; Human papillomavirus ; Humans ; Immunohistochemistry ; In Situ Hybridization ; Infections ; Male ; Middle Aged ; Morphology ; Mouth Neoplasms - chemistry ; Mouth Neoplasms - pathology ; Mouth Neoplasms - virology ; oral ; oral pathology ; Papillomaviridae - genetics ; Papillomavirus Infections - complications ; Papillomavirus Infections - virology ; Polymerase Chain Reaction ; squamous cell carcinoma ; Tonsillitis ; Verrucous</subject><ispartof>Journal of clinical pathology, 2012-03, Vol.65 (3), p.283-286</ispartof><rights>2012, Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.</rights><rights>Copyright: 2012 (c) 2012, Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b447t-62657c27ab9f1c8496e1e6550a82792304dda66dbc7b0b4a7f655d4554685d263</citedby><cites>FETCH-LOGICAL-b447t-62657c27ab9f1c8496e1e6550a82792304dda66dbc7b0b4a7f655d4554685d263</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://jcp.bmj.com/content/65/3/283.full.pdf$$EPDF$$P50$$Gbmj$$H</linktopdf><linktohtml>$$Uhttps://jcp.bmj.com/content/65/3/283.full$$EHTML$$P50$$Gbmj$$H</linktohtml><link.rule.ids>114,115,314,780,784,3196,23571,27924,27925,77600,77631</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22174425$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stokes, Angela</creatorcontrib><creatorcontrib>Guerra, Eliete</creatorcontrib><creatorcontrib>Bible, Jon</creatorcontrib><creatorcontrib>Halligan, Eugene</creatorcontrib><creatorcontrib>Orchard, Guy</creatorcontrib><creatorcontrib>Odell, Edward</creatorcontrib><creatorcontrib>Thavaraj, Selvam</creatorcontrib><title>Human papillomavirus detection in dysplastic and malignant oral verrucous lesions</title><title>Journal of clinical pathology</title><addtitle>J Clin Pathol</addtitle><description>The role of human papillomaviruses (HPV) in dysplastic and malignant oral verrucous lesions is controversial since there is a wide range in the incidence of virus detection. This study used a multi-tiered method of HPV detection using DNA in-situ hybridisation (ISH) for low- and high-risk subtypes, consensus PCR, and HPV genotype analysis in archival tissue from 20 cases of dysplastic and malignant oral verrucous lesions. The biological significance of HPV DNA detection was assessed by p16 immunohistochemistry (IHC). While 1/7 carcinomas and 5/13 dysplasias contained HPV DNA by consensus PCR and genotype analysis, all specimens were negative for low- and high-risk HPV ISH and negative for p16 IHC. Results show that although high-risk HPV DNA is detectable in a subset of these lesions, the lack of p16 overexpression suggests that the oncogenic process is not driven by HPV oncoproteins.</description><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biomarkers, Tumor - analysis</subject><subject>cancer</subject><subject>cancer research</subject><subject>Carcinoma, Verrucous - chemistry</subject><subject>Carcinoma, Verrucous - pathology</subject><subject>Carcinoma, Verrucous - virology</subject><subject>Cervical cancer</subject><subject>Cyclin-Dependent Kinase Inhibitor p16 - analysis</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Viral - isolation & purification</subject><subject>dysplasia</subject><subject>Female</subject><subject>Genotype</subject><subject>head and neck</subject><subject>head and neck cancer</subject><subject>HPV</subject><subject>Human papillomavirus</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>In Situ Hybridization</subject><subject>Infections</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Morphology</subject><subject>Mouth Neoplasms - chemistry</subject><subject>Mouth Neoplasms - pathology</subject><subject>Mouth Neoplasms - virology</subject><subject>oral</subject><subject>oral pathology</subject><subject>Papillomaviridae - genetics</subject><subject>Papillomavirus Infections - complications</subject><subject>Papillomavirus Infections - virology</subject><subject>Polymerase Chain Reaction</subject><subject>squamous cell carcinoma</subject><subject>Tonsillitis</subject><subject>Verrucous</subject><issn>0021-9746</issn><issn>1472-4146</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkU9v1DAQxS0EokvhI4AiceAU6nH8Z3OEFaWoK6qiwtWa2F7w4jjBTir67fEqZQ-9wGXmML8380aPkJdA3wI08mxvgo8jTj9qRgFKoVzwR2QFXLGaA5ePyYpSBnWruDwhz3LeUwqNguYpOWEMFOdMrMj1xdxjrEYcfQhDj7c-zbmybnJm8kOsfKzsXR4D5smbCqOtegz-e8Q4VUPCUN26lGYzFFFwuSjyc_JkhyG7F_f9lHw9_3Czuai3Vx8_bd5t645zNdWSSaEMU9i1OzBr3koHTgpBcc1UyxrKrUUpbWdURzuOaleGlgvB5VpYJptT8mbZO6bh1-zypHufjQsBoyt2dFtIAUXwb5Kxcl41TSFfPyD3w5xieUODWgPljLWHy2KhTBpyTm6nx-R7THcaqD6Eo4_h6EM4egmn6F7db5-73tmj6m8aBagXwOfJ_T7OMf3UxZ4S-vO3jT7_cgnvtzeXWhWeLnzX7__Twx-Gbqsj</recordid><startdate>20120301</startdate><enddate>20120301</enddate><creator>Stokes, Angela</creator><creator>Guerra, Eliete</creator><creator>Bible, Jon</creator><creator>Halligan, Eugene</creator><creator>Orchard, Guy</creator><creator>Odell, Edward</creator><creator>Thavaraj, Selvam</creator><general>BMJ Publishing Group Ltd and Association of Clinical Pathologists</general><general>BMJ Publishing Group LTD</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BTHHO</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>20120301</creationdate><title>Human papillomavirus detection in dysplastic and malignant oral verrucous lesions</title><author>Stokes, Angela ; Guerra, Eliete ; Bible, Jon ; Halligan, Eugene ; Orchard, Guy ; Odell, Edward ; Thavaraj, Selvam</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b447t-62657c27ab9f1c8496e1e6550a82792304dda66dbc7b0b4a7f655d4554685d263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biomarkers, Tumor - 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complications</topic><topic>Papillomavirus Infections - virology</topic><topic>Polymerase Chain Reaction</topic><topic>squamous cell carcinoma</topic><topic>Tonsillitis</topic><topic>Verrucous</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stokes, Angela</creatorcontrib><creatorcontrib>Guerra, Eliete</creatorcontrib><creatorcontrib>Bible, Jon</creatorcontrib><creatorcontrib>Halligan, Eugene</creatorcontrib><creatorcontrib>Orchard, Guy</creatorcontrib><creatorcontrib>Odell, Edward</creatorcontrib><creatorcontrib>Thavaraj, Selvam</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>BMJ Journals</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database (ProQuest)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Journal of clinical pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stokes, Angela</au><au>Guerra, Eliete</au><au>Bible, Jon</au><au>Halligan, Eugene</au><au>Orchard, Guy</au><au>Odell, Edward</au><au>Thavaraj, Selvam</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human papillomavirus detection in dysplastic and malignant oral verrucous lesions</atitle><jtitle>Journal of clinical pathology</jtitle><addtitle>J Clin Pathol</addtitle><date>2012-03-01</date><risdate>2012</risdate><volume>65</volume><issue>3</issue><spage>283</spage><epage>286</epage><pages>283-286</pages><issn>0021-9746</issn><eissn>1472-4146</eissn><coden>JCPAAK</coden><abstract>The role of human papillomaviruses (HPV) in dysplastic and malignant oral verrucous lesions is controversial since there is a wide range in the incidence of virus detection. This study used a multi-tiered method of HPV detection using DNA in-situ hybridisation (ISH) for low- and high-risk subtypes, consensus PCR, and HPV genotype analysis in archival tissue from 20 cases of dysplastic and malignant oral verrucous lesions. The biological significance of HPV DNA detection was assessed by p16 immunohistochemistry (IHC). While 1/7 carcinomas and 5/13 dysplasias contained HPV DNA by consensus PCR and genotype analysis, all specimens were negative for low- and high-risk HPV ISH and negative for p16 IHC. Results show that although high-risk HPV DNA is detectable in a subset of these lesions, the lack of p16 overexpression suggests that the oncogenic process is not driven by HPV oncoproteins.</abstract><cop>England</cop><pub>BMJ Publishing Group Ltd and Association of Clinical Pathologists</pub><pmid>22174425</pmid><doi>10.1136/jclinpath-2011-200454</doi><tpages>4</tpages></addata></record> |
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| subjects | Aged Aged, 80 and over Biomarkers, Tumor - analysis cancer cancer research Carcinoma, Verrucous - chemistry Carcinoma, Verrucous - pathology Carcinoma, Verrucous - virology Cervical cancer Cyclin-Dependent Kinase Inhibitor p16 - analysis Deoxyribonucleic acid DNA DNA, Viral - isolation & purification dysplasia Female Genotype head and neck head and neck cancer HPV Human papillomavirus Humans Immunohistochemistry In Situ Hybridization Infections Male Middle Aged Morphology Mouth Neoplasms - chemistry Mouth Neoplasms - pathology Mouth Neoplasms - virology oral oral pathology Papillomaviridae - genetics Papillomavirus Infections - complications Papillomavirus Infections - virology Polymerase Chain Reaction squamous cell carcinoma Tonsillitis Verrucous |
| title | Human papillomavirus detection in dysplastic and malignant oral verrucous lesions |
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