Modulation of activated oncogene c-kit expression with RNA-interference
Overexpression of oncogene c-kit is detected in 80% patients with acute myeloid leukemia (AML). A transgenic model cell line expressing oncogene c-kit was obtained by transduction with a recombinant retrovirus. We have designed small interfering RNAs (siRNAs) that efficiently suppress the expression...
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| Veröffentlicht in: | Molecular biology (New York) 2011-12, Vol.45 (6), p.950-958 |
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| container_title | Molecular biology (New York) |
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| creator | Spirin, P. V. Nikitenko, N. A. Lebedev, T. D. Rubtsov, P. M. Stocking, C. Prasolov, V. S. |
| description | Overexpression of oncogene
c-kit
is detected in 80% patients with acute myeloid leukemia (AML). A transgenic model cell line expressing oncogene
c-kit
was obtained by transduction with a recombinant retrovirus. We have designed small interfering RNAs (siRNAs) that efficiently suppress the expression of activated oncogene
c-kit
. Further, small hairpin RNAs (shRNAs) targeting
c-kit
mRNA were designed and expressed in lentiviral vectors. We report a stable reduction in
c-kit
expression following the introduction of shRNAs into model cells, as well as Kasumi-1 cells from a patient with AML. |
| doi_str_mv | 10.1134/S0026893311060136 |
| format | Article |
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c-kit
is detected in 80% patients with acute myeloid leukemia (AML). A transgenic model cell line expressing oncogene
c-kit
was obtained by transduction with a recombinant retrovirus. We have designed small interfering RNAs (siRNAs) that efficiently suppress the expression of activated oncogene
c-kit
. Further, small hairpin RNAs (shRNAs) targeting
c-kit
mRNA were designed and expressed in lentiviral vectors. We report a stable reduction in
c-kit
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c-kit
is detected in 80% patients with acute myeloid leukemia (AML). A transgenic model cell line expressing oncogene
c-kit
was obtained by transduction with a recombinant retrovirus. We have designed small interfering RNAs (siRNAs) that efficiently suppress the expression of activated oncogene
c-kit
. Further, small hairpin RNAs (shRNAs) targeting
c-kit
mRNA were designed and expressed in lentiviral vectors. We report a stable reduction in
c-kit
expression following the introduction of shRNAs into model cells, as well as Kasumi-1 cells from a patient with AML.</description><subject>Acute myeloid leukemia</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>c-Kit protein</subject><subject>Cell culture</subject><subject>Gene expression</subject><subject>Human Genetics</subject><subject>Leukemia</subject><subject>Life Sciences</subject><subject>Molecular biology</subject><subject>Molecular Biology of the Cell</subject><subject>mRNA</subject><subject>Oncogenes</subject><subject>Retrovirus</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>siRNA</subject><issn>0026-8933</issn><issn>1608-3245</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kEtLAzEUhYMoWKs_wN3gxtVoXpPJLEvRKlQFH-shTW7q1GlSk4yPf-8MFQRFuHAW5zuHy0HomOAzQhg_f8CYClkxRggWmDCxg0ZEYJkzyotdNBrsfPD30UGMK4xJf3SEZjfedK1KjXeZt5nSqXlTCUzmnfZLcJDp_KVJGXxsAsQ4YO9Nes7ubyd54xIECwGchkO0Z1Ub4ehbx-jp8uJxepXP72bX08k814yXKZe0pKXiRBOsrOZam9IwKqUhFbba2kpXxhgmuSLUyIWUumDMlAthQQmGJRuj023vJvjXDmKq103U0LbKge9iXRVcsJJT3pMnv8iV74Lrn6srXBaSEyp6iGwhHXyMAWy9Cc1ahc-a4HoYtv4zbJ-h20zsWbeE8FP8f-gLn7J5lg</recordid><startdate>20111201</startdate><enddate>20111201</enddate><creator>Spirin, P. 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c-kit
is detected in 80% patients with acute myeloid leukemia (AML). A transgenic model cell line expressing oncogene
c-kit
was obtained by transduction with a recombinant retrovirus. We have designed small interfering RNAs (siRNAs) that efficiently suppress the expression of activated oncogene
c-kit
. Further, small hairpin RNAs (shRNAs) targeting
c-kit
mRNA were designed and expressed in lentiviral vectors. We report a stable reduction in
c-kit
expression following the introduction of shRNAs into model cells, as well as Kasumi-1 cells from a patient with AML.</abstract><cop>Dordrecht</cop><pub>SP MAIK Nauka/Interperiodica</pub><doi>10.1134/S0026893311060136</doi><tpages>9</tpages></addata></record> |
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| subjects | Acute myeloid leukemia Biochemistry Biomedical and Life Sciences c-Kit protein Cell culture Gene expression Human Genetics Leukemia Life Sciences Molecular biology Molecular Biology of the Cell mRNA Oncogenes Retrovirus Ribonucleic acid RNA siRNA |
| title | Modulation of activated oncogene c-kit expression with RNA-interference |
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