Ultrasound Guided Transplantation of Enriched and Cryopreserved Spermatogonial Cell Suspension in Goats
Spermatogonial stem cells transplantation provides a unique approach for studying spermatogenesis. Initially developed in mice, this technique has now been extended in farm animals and provides an alternative means to preserve valuable male germ line and to produce transgenic animals. The aim of thi...
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| Veröffentlicht in: | Reproduction in domestic animals 2010-12, Vol.45 (6), p.e249-e254 |
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| creator | Kaul, G Kaur, J Rafeeqi, TA |
| description | Spermatogonial stem cells transplantation provides a unique approach for studying spermatogenesis. Initially developed in mice, this technique has now been extended in farm animals and provides an alternative means to preserve valuable male germ line and to produce transgenic animals. The aim of this study was to enrich type A spermatogonial cells amongst the isolated cells from goat testis, to cryopreserve these enriched populations of cells and their subsequent transplantation in unrelated recipient goats under ultrasound guidance. The cells were isolated enzymatically and enriched by differential plating and separation on discontinuous percoll gradient. Ultrasound guided injection of trypan blue dye into rete testis resulted in 20-30% filling of the seminiferous tubules. Prior to transplantation, the cells were labelled with a fluorescent dye to trace donor cells in recipient seminiferous tubules after transplantation. The fluorescent‐labelled cells were observed up to 12 weeks after transplantation. |
| doi_str_mv | 10.1111/j.1439-0531.2009.01549.x |
| format | Article |
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Initially developed in mice, this technique has now been extended in farm animals and provides an alternative means to preserve valuable male germ line and to produce transgenic animals. The aim of this study was to enrich type A spermatogonial cells amongst the isolated cells from goat testis, to cryopreserve these enriched populations of cells and their subsequent transplantation in unrelated recipient goats under ultrasound guidance. The cells were isolated enzymatically and enriched by differential plating and separation on discontinuous percoll gradient. Ultrasound guided injection of trypan blue dye into rete testis resulted in 20-30% filling of the seminiferous tubules. Prior to transplantation, the cells were labelled with a fluorescent dye to trace donor cells in recipient seminiferous tubules after transplantation. The fluorescent‐labelled cells were observed up to 12 weeks after transplantation.</description><identifier>ISSN: 0936-6768</identifier><identifier>EISSN: 1439-0531</identifier><identifier>DOI: 10.1111/j.1439-0531.2009.01549.x</identifier><identifier>PMID: 19961552</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Cell suspensions ; Cryopreservation ; Cryopreservation - veterinary ; Farms ; Fluorescent indicators ; Goats - physiology ; Male ; Rodents ; Seminiferous tubule ; Spermatogenesis ; Spermatogonia - physiology ; Stem Cell Transplantation - methods ; Stem Cell Transplantation - veterinary ; Testes ; Testis - physiology ; Transgenic animals ; Transplants & implants ; Ultrasound</subject><ispartof>Reproduction in domestic animals, 2010-12, Vol.45 (6), p.e249-e254</ispartof><rights>2009 Blackwell Verlag GmbH</rights><rights>2009 Blackwell Verlag GmbH.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4899-b5f7b88b6cfd393adff89ebdc85e821656522d7398678b84c2033f9cdd6834583</citedby><cites>FETCH-LOGICAL-c4899-b5f7b88b6cfd393adff89ebdc85e821656522d7398678b84c2033f9cdd6834583</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1439-0531.2009.01549.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1439-0531.2009.01549.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19961552$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kaul, G</creatorcontrib><creatorcontrib>Kaur, J</creatorcontrib><creatorcontrib>Rafeeqi, TA</creatorcontrib><title>Ultrasound Guided Transplantation of Enriched and Cryopreserved Spermatogonial Cell Suspension in Goats</title><title>Reproduction in domestic animals</title><addtitle>Reprod Domest Anim</addtitle><description>Spermatogonial stem cells transplantation provides a unique approach for studying spermatogenesis. Initially developed in mice, this technique has now been extended in farm animals and provides an alternative means to preserve valuable male germ line and to produce transgenic animals. The aim of this study was to enrich type A spermatogonial cells amongst the isolated cells from goat testis, to cryopreserve these enriched populations of cells and their subsequent transplantation in unrelated recipient goats under ultrasound guidance. The cells were isolated enzymatically and enriched by differential plating and separation on discontinuous percoll gradient. Ultrasound guided injection of trypan blue dye into rete testis resulted in 20-30% filling of the seminiferous tubules. Prior to transplantation, the cells were labelled with a fluorescent dye to trace donor cells in recipient seminiferous tubules after transplantation. The fluorescent‐labelled cells were observed up to 12 weeks after transplantation.</description><subject>Animals</subject><subject>Cell suspensions</subject><subject>Cryopreservation</subject><subject>Cryopreservation - veterinary</subject><subject>Farms</subject><subject>Fluorescent indicators</subject><subject>Goats - physiology</subject><subject>Male</subject><subject>Rodents</subject><subject>Seminiferous tubule</subject><subject>Spermatogenesis</subject><subject>Spermatogonia - physiology</subject><subject>Stem Cell Transplantation - methods</subject><subject>Stem Cell Transplantation - veterinary</subject><subject>Testes</subject><subject>Testis - physiology</subject><subject>Transgenic animals</subject><subject>Transplants & implants</subject><subject>Ultrasound</subject><issn>0936-6768</issn><issn>1439-0531</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc2O0zAURi0EYkrhFSBiwyrBjv8XLEadoSBGIGg7sLOc2CkuaRzsBNq3x6HVILEBb2z5nu_q2geADMECpfVyVyCCZQ4pRkUJoSwgokQWh3tgdle4D2ZQYpYzzsQFeBTjDiZKcP4QXCApGaK0nIHtph2Cjn7sTLYcnbEmWwfdxb7V3aAH57vMN9l1F1z9NdV0whbh6Ptgow0_0s2qt2GvB7_1ndNttrBtm63G2NsuTmHXZUuvh_gYPGh0G-2T8z4Hm9fX68Wb_ObD8u3i8iaviZAyr2jDKyEqVjcGS6xN0whpK1MLakWJGGW0LA3HUjAuKkHqEmLcyNoYJjChAs_Bi1PfPvjvo42D2rtYp6F0Z_0YlaSEIYxk-U9SQEEYZpQm8vlf5M6PoUvPSBAnkNP00XMgTlAdfIzBNqoPbq_DUSGoJmlqpyY3anKjJmnqtzR1SNGn5_5jtbfmT_BsKQGvTsBP19rjfzdWn64up1PK56e8i4M93OV1-KYYx5yqz--X6gtZ89vbd1h9TPyzE99or_Q2uKg2qxIiDJFEFBGKfwGVN74D</recordid><startdate>201012</startdate><enddate>201012</enddate><creator>Kaul, G</creator><creator>Kaur, J</creator><creator>Rafeeqi, TA</creator><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201012</creationdate><title>Ultrasound Guided Transplantation of Enriched and Cryopreserved Spermatogonial Cell Suspension in Goats</title><author>Kaul, G ; Kaur, J ; Rafeeqi, TA</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4899-b5f7b88b6cfd393adff89ebdc85e821656522d7398678b84c2033f9cdd6834583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Cell suspensions</topic><topic>Cryopreservation</topic><topic>Cryopreservation - veterinary</topic><topic>Farms</topic><topic>Fluorescent indicators</topic><topic>Goats - physiology</topic><topic>Male</topic><topic>Rodents</topic><topic>Seminiferous tubule</topic><topic>Spermatogenesis</topic><topic>Spermatogonia - physiology</topic><topic>Stem Cell Transplantation - methods</topic><topic>Stem Cell Transplantation - veterinary</topic><topic>Testes</topic><topic>Testis - physiology</topic><topic>Transgenic animals</topic><topic>Transplants & implants</topic><topic>Ultrasound</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kaul, G</creatorcontrib><creatorcontrib>Kaur, J</creatorcontrib><creatorcontrib>Rafeeqi, TA</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Reproduction in domestic animals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kaul, G</au><au>Kaur, J</au><au>Rafeeqi, TA</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultrasound Guided Transplantation of Enriched and Cryopreserved Spermatogonial Cell Suspension in Goats</atitle><jtitle>Reproduction in domestic animals</jtitle><addtitle>Reprod Domest Anim</addtitle><date>2010-12</date><risdate>2010</risdate><volume>45</volume><issue>6</issue><spage>e249</spage><epage>e254</epage><pages>e249-e254</pages><issn>0936-6768</issn><eissn>1439-0531</eissn><abstract>Spermatogonial stem cells transplantation provides a unique approach for studying spermatogenesis. Initially developed in mice, this technique has now been extended in farm animals and provides an alternative means to preserve valuable male germ line and to produce transgenic animals. The aim of this study was to enrich type A spermatogonial cells amongst the isolated cells from goat testis, to cryopreserve these enriched populations of cells and their subsequent transplantation in unrelated recipient goats under ultrasound guidance. The cells were isolated enzymatically and enriched by differential plating and separation on discontinuous percoll gradient. Ultrasound guided injection of trypan blue dye into rete testis resulted in 20-30% filling of the seminiferous tubules. Prior to transplantation, the cells were labelled with a fluorescent dye to trace donor cells in recipient seminiferous tubules after transplantation. 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| ispartof | Reproduction in domestic animals, 2010-12, Vol.45 (6), p.e249-e254 |
| issn | 0936-6768 1439-0531 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Cell suspensions Cryopreservation Cryopreservation - veterinary Farms Fluorescent indicators Goats - physiology Male Rodents Seminiferous tubule Spermatogenesis Spermatogonia - physiology Stem Cell Transplantation - methods Stem Cell Transplantation - veterinary Testes Testis - physiology Transgenic animals Transplants & implants Ultrasound |
| title | Ultrasound Guided Transplantation of Enriched and Cryopreserved Spermatogonial Cell Suspension in Goats |
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