Detoxification of aflatoxin B1 by manganese peroxidase from the white-rot fungus Phanerochaete sordida YK-624
Abstract Aflatoxin B1 (AFB1) is a potent mycotoxin with mutagenic, carcinogenic, teratogenic, hepatotoxic, and immunosuppressive properties. In order to develop a bioremediation system for AFB1-contaminated foods by white-rot fungi or ligninolytic enzymes, AFB1 was treated with manganese peroxidase...
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| Veröffentlicht in: | FEMS microbiology letters 2011-01, Vol.314 (2), p.164-169 |
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| creator | Wang, Jianqiao Ogata, Makoto Hirai, Hirofumi Kawagishi, Hirokazu |
| description | Abstract
Aflatoxin B1 (AFB1) is a potent mycotoxin with mutagenic, carcinogenic, teratogenic, hepatotoxic, and immunosuppressive properties. In order to develop a bioremediation system for AFB1-contaminated foods by white-rot fungi or ligninolytic enzymes, AFB1 was treated with manganese peroxidase (MnP) from the white-rot fungus Phanerochaete sordida YK-624. AFB1 was eliminated by MnP. The maximum elimination (86.0%) of AFB1 was observed after 48 h in a reaction mixture containing 5 nkat of MnP. The addition of Tween 80 enhanced AFB1 elimination. The elimination of AFB1 by MnP considerably reduced its mutagenic activity in an umu test, and the treatment of AFB1 by 20 nkat MnP reduced the mutagenic activity by 69.2%. 1H-NMR and HR-ESI-MS analysis suggested that AFB1 is first oxidized to AFB1-8,9-epoxide by MnP and then hydrolyzed to AFB1-8,9-dihydrodiol. This is the first report that MnP can effectively remove the mutagenic activity of AFB1 by converting it into AFB1-8,9-dihydrodiol. |
| doi_str_mv | 10.1111/j.1574-6968.2010.02158.x |
| format | Article |
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Aflatoxin B1 (AFB1) is a potent mycotoxin with mutagenic, carcinogenic, teratogenic, hepatotoxic, and immunosuppressive properties. In order to develop a bioremediation system for AFB1-contaminated foods by white-rot fungi or ligninolytic enzymes, AFB1 was treated with manganese peroxidase (MnP) from the white-rot fungus Phanerochaete sordida YK-624. AFB1 was eliminated by MnP. The maximum elimination (86.0%) of AFB1 was observed after 48 h in a reaction mixture containing 5 nkat of MnP. The addition of Tween 80 enhanced AFB1 elimination. The elimination of AFB1 by MnP considerably reduced its mutagenic activity in an umu test, and the treatment of AFB1 by 20 nkat MnP reduced the mutagenic activity by 69.2%. 1H-NMR and HR-ESI-MS analysis suggested that AFB1 is first oxidized to AFB1-8,9-epoxide by MnP and then hydrolyzed to AFB1-8,9-dihydrodiol. This is the first report that MnP can effectively remove the mutagenic activity of AFB1 by converting it into AFB1-8,9-dihydrodiol.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.2010.02158.x</identifier><identifier>PMID: 21118293</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Aflatoxin B1 ; Aflatoxin B1 - metabolism ; Aflatoxins ; Biological and medical sciences ; Bioremediation ; Biotransformation ; Carcinogens ; Detoxification ; Enzyme Activators - metabolism ; Food contamination ; Fundamental and applied biological sciences. Psychology ; Fungi ; Ligninolytic enzymes ; Magnetic Resonance Spectroscopy ; Manganese ; Manganese peroxidase ; Microbiology ; Miscellaneous ; mutagenic activity ; Mutagens - metabolism ; Mycology ; Mycotoxins ; NMR ; Nuclear magnetic resonance ; Peroxidase ; Peroxidases - metabolism ; Phanerochaete ; Phanerochaete - enzymology ; Phanerochaete sordida ; Phanerochaete sordida YK‐624 ; Polysorbates - metabolism ; Teratogenicity ; White rot ; White rot fungi</subject><ispartof>FEMS microbiology letters, 2011-01, Vol.314 (2), p.164-169</ispartof><rights>2010 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd. All rights reserved 2010</rights><rights>2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved</rights><rights>2015 INIST-CNRS</rights><rights>2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.</rights><rights>2010 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd. All rights reserved</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4848-95e62bdcdc8ddee512b40a871b894a19e726c9e1e5f01441312cdee1acfebf873</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6968.2010.02158.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6968.2010.02158.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23699660$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21118293$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Jianqiao</creatorcontrib><creatorcontrib>Ogata, Makoto</creatorcontrib><creatorcontrib>Hirai, Hirofumi</creatorcontrib><creatorcontrib>Kawagishi, Hirokazu</creatorcontrib><title>Detoxification of aflatoxin B1 by manganese peroxidase from the white-rot fungus Phanerochaete sordida YK-624</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Abstract
Aflatoxin B1 (AFB1) is a potent mycotoxin with mutagenic, carcinogenic, teratogenic, hepatotoxic, and immunosuppressive properties. In order to develop a bioremediation system for AFB1-contaminated foods by white-rot fungi or ligninolytic enzymes, AFB1 was treated with manganese peroxidase (MnP) from the white-rot fungus Phanerochaete sordida YK-624. AFB1 was eliminated by MnP. The maximum elimination (86.0%) of AFB1 was observed after 48 h in a reaction mixture containing 5 nkat of MnP. The addition of Tween 80 enhanced AFB1 elimination. The elimination of AFB1 by MnP considerably reduced its mutagenic activity in an umu test, and the treatment of AFB1 by 20 nkat MnP reduced the mutagenic activity by 69.2%. 1H-NMR and HR-ESI-MS analysis suggested that AFB1 is first oxidized to AFB1-8,9-epoxide by MnP and then hydrolyzed to AFB1-8,9-dihydrodiol. This is the first report that MnP can effectively remove the mutagenic activity of AFB1 by converting it into AFB1-8,9-dihydrodiol.</description><subject>Aflatoxin B1</subject><subject>Aflatoxin B1 - metabolism</subject><subject>Aflatoxins</subject><subject>Biological and medical sciences</subject><subject>Bioremediation</subject><subject>Biotransformation</subject><subject>Carcinogens</subject><subject>Detoxification</subject><subject>Enzyme Activators - metabolism</subject><subject>Food contamination</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungi</subject><subject>Ligninolytic enzymes</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Manganese</subject><subject>Manganese peroxidase</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>mutagenic activity</subject><subject>Mutagens - metabolism</subject><subject>Mycology</subject><subject>Mycotoxins</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>Peroxidase</subject><subject>Peroxidases - metabolism</subject><subject>Phanerochaete</subject><subject>Phanerochaete - enzymology</subject><subject>Phanerochaete sordida</subject><subject>Phanerochaete sordida YK‐624</subject><subject>Polysorbates - metabolism</subject><subject>Teratogenicity</subject><subject>White rot</subject><subject>White rot fungi</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kUFv1DAQhS0EokvhLyBLCHHKMnYcxz5wgEIBUQQHOHCyHGfczSqJt3ai7v57HHYpEghfPHrzvdFoHiGUwZrl93K7ZlUtCqmlWnPIKnBWqfX-HlndNe6TFZS1Khjo-ow8SmkLAIKDfEjOeB6iuC5XZHiLU9h3vnN26sJIg6fW93bRRvqG0eZABzte2xET0h3GrLc2lz6GgU4bpLebbsIihon6ebyeE_26yXAMbmNxQppCbLOD_vhUSC4ekwfe9gmfnP5z8v3y3beLD8XVl_cfL15fFU4ooQpdoeRN61qn2haxYrwRYFXNGqWFZRprLp1GhpUHJgQrGXeZY9Z5bLyqy3Py4jh3F8PNjGkyQ5cc9n1eLczJ6EpI0BWwTD77i9yGOY55OcNLqJQG0Av19ETNzYCt2cVusPFgft8xA89PgE3O9j7a0XXpD1dKraWEzL06crddj4e7PgOz5Gq2ZonPLPGZJVfzK1ezN5efr5Yq-8ujP8y7_7iLf9zlTxsbpJ0</recordid><startdate>201101</startdate><enddate>201101</enddate><creator>Wang, Jianqiao</creator><creator>Ogata, Makoto</creator><creator>Hirai, Hirofumi</creator><creator>Kawagishi, Hirokazu</creator><general>Blackwell Publishing Ltd</general><general>Wiley-Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7ST</scope><scope>SOI</scope></search><sort><creationdate>201101</creationdate><title>Detoxification of aflatoxin B1 by manganese peroxidase from the white-rot fungus Phanerochaete sordida YK-624</title><author>Wang, Jianqiao ; Ogata, Makoto ; Hirai, Hirofumi ; Kawagishi, Hirokazu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4848-95e62bdcdc8ddee512b40a871b894a19e726c9e1e5f01441312cdee1acfebf873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Aflatoxin B1</topic><topic>Aflatoxin B1 - metabolism</topic><topic>Aflatoxins</topic><topic>Biological and medical sciences</topic><topic>Bioremediation</topic><topic>Biotransformation</topic><topic>Carcinogens</topic><topic>Detoxification</topic><topic>Enzyme Activators - metabolism</topic><topic>Food contamination</topic><topic>Fundamental and applied biological sciences. 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Aflatoxin B1 (AFB1) is a potent mycotoxin with mutagenic, carcinogenic, teratogenic, hepatotoxic, and immunosuppressive properties. In order to develop a bioremediation system for AFB1-contaminated foods by white-rot fungi or ligninolytic enzymes, AFB1 was treated with manganese peroxidase (MnP) from the white-rot fungus Phanerochaete sordida YK-624. AFB1 was eliminated by MnP. The maximum elimination (86.0%) of AFB1 was observed after 48 h in a reaction mixture containing 5 nkat of MnP. The addition of Tween 80 enhanced AFB1 elimination. The elimination of AFB1 by MnP considerably reduced its mutagenic activity in an umu test, and the treatment of AFB1 by 20 nkat MnP reduced the mutagenic activity by 69.2%. 1H-NMR and HR-ESI-MS analysis suggested that AFB1 is first oxidized to AFB1-8,9-epoxide by MnP and then hydrolyzed to AFB1-8,9-dihydrodiol. This is the first report that MnP can effectively remove the mutagenic activity of AFB1 by converting it into AFB1-8,9-dihydrodiol.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>21118293</pmid><doi>10.1111/j.1574-6968.2010.02158.x</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Aflatoxin B1 Aflatoxin B1 - metabolism Aflatoxins Biological and medical sciences Bioremediation Biotransformation Carcinogens Detoxification Enzyme Activators - metabolism Food contamination Fundamental and applied biological sciences. Psychology Fungi Ligninolytic enzymes Magnetic Resonance Spectroscopy Manganese Manganese peroxidase Microbiology Miscellaneous mutagenic activity Mutagens - metabolism Mycology Mycotoxins NMR Nuclear magnetic resonance Peroxidase Peroxidases - metabolism Phanerochaete Phanerochaete - enzymology Phanerochaete sordida Phanerochaete sordida YK‐624 Polysorbates - metabolism Teratogenicity White rot White rot fungi |
| title | Detoxification of aflatoxin B1 by manganese peroxidase from the white-rot fungus Phanerochaete sordida YK-624 |
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