In vitro induction and identification of autotetraploids of Dioscorea zingiberensis
Dioscorea zingiberensis is an important medicinal plant and a source of diosgenin in China. We report research on the induction, characteristics, and chemical assays of polyploid plants of D. zingiberensis. Immersing calli in 0.3% colchicine solution for 16 h prior to culture induced a high number o...
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creator | Heping, Huang Shanlin, Gao Lanlan, Chen Xiaoke, Jiao |
description | Dioscorea zingiberensis is an important medicinal plant and a source of diosgenin in China. We report research on the induction, characteristics, and chemical assays of polyploid plants of D. zingiberensis. Immersing calli in 0.3% colchicine solution for 16 h prior to culture induced a high number of autotetraploid plants. The induction rate reached as high as 36.7% of treated calli. More than 50 lines of autotetraploid plants were obtained. All tetraploid plants showed typical polyploidy characteristics. Twenty selected tetraploid lines were transferred to the field for determination of morphological characteristics and for chemical assays. Six elite lines have been selected for further selection and breeding into new varieties for commercial production. |
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We report research on the induction, characteristics, and chemical assays of polyploid plants of D. zingiberensis. Immersing calli in 0.3% colchicine solution for 16 h prior to culture induced a high number of autotetraploid plants. The induction rate reached as high as 36.7% of treated calli. More than 50 lines of autotetraploid plants were obtained. All tetraploid plants showed typical polyploidy characteristics. Twenty selected tetraploid lines were transferred to the field for determination of morphological characteristics and for chemical assays. 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Plant</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Heping, Huang</au><au>Shanlin, Gao</au><au>Lanlan, Chen</au><au>Xiaoke, Jiao</au><au>Altman, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro induction and identification of autotetraploids of Dioscorea zingiberensis</atitle><jtitle>In vitro cellular & developmental biology. Plant</jtitle><stitle>In Vitro Cell.Dev.Biol.-Plant</stitle><date>2008-10-01</date><risdate>2008</risdate><volume>44</volume><issue>5</issue><spage>448</spage><epage>455</epage><pages>448-455</pages><issn>1054-5476</issn><eissn>1475-2689</eissn><abstract>Dioscorea zingiberensis is an important medicinal plant and a source of diosgenin in China. We report research on the induction, characteristics, and chemical assays of polyploid plants of D. zingiberensis. Immersing calli in 0.3% colchicine solution for 16 h prior to culture induced a high number of autotetraploid plants. The induction rate reached as high as 36.7% of treated calli. More than 50 lines of autotetraploid plants were obtained. All tetraploid plants showed typical polyploidy characteristics. Twenty selected tetraploid lines were transferred to the field for determination of morphological characteristics and for chemical assays. Six elite lines have been selected for further selection and breeding into new varieties for commercial production.</abstract><cop>New York</cop><pub>The Society for In Vitro Biology</pub><doi>10.1007/s11627-008-9177-3</doi><tpages>8</tpages></addata></record> |
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subjects | Biomedical and Life Sciences Callus Cell Biology Chromosomes Colchicine Developmental Biology Dioscorea zingiberensis Diploidy Leaves Life Sciences Medicinal plant Medicinal plants Pharmaceutical industry Plant Breeding/Biotechnology Plant Genetics and Genomics Plant Sciences Plantlets Plants Polyploidy Research Reports Rhizomes Seeds Stomata Surface water Tetraploid Tetraploidy |
title | In vitro induction and identification of autotetraploids of Dioscorea zingiberensis |
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