In vitro induction and identification of autotetraploids of Dioscorea zingiberensis

Dioscorea zingiberensis is an important medicinal plant and a source of diosgenin in China. We report research on the induction, characteristics, and chemical assays of polyploid plants of D. zingiberensis. Immersing calli in 0.3% colchicine solution for 16 h prior to culture induced a high number o...

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Veröffentlicht in:In vitro cellular & developmental biology. Plant 2008-10, Vol.44 (5), p.448-455
Hauptverfasser: Heping, Huang, Shanlin, Gao, Lanlan, Chen, Xiaoke, Jiao
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container_issue 5
container_start_page 448
container_title In vitro cellular & developmental biology. Plant
container_volume 44
creator Heping, Huang
Shanlin, Gao
Lanlan, Chen
Xiaoke, Jiao
description Dioscorea zingiberensis is an important medicinal plant and a source of diosgenin in China. We report research on the induction, characteristics, and chemical assays of polyploid plants of D. zingiberensis. Immersing calli in 0.3% colchicine solution for 16 h prior to culture induced a high number of autotetraploid plants. The induction rate reached as high as 36.7% of treated calli. More than 50 lines of autotetraploid plants were obtained. All tetraploid plants showed typical polyploidy characteristics. Twenty selected tetraploid lines were transferred to the field for determination of morphological characteristics and for chemical assays. Six elite lines have been selected for further selection and breeding into new varieties for commercial production.
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subjects Biomedical and Life Sciences
Callus
Cell Biology
Chromosomes
Colchicine
Developmental Biology
Dioscorea zingiberensis
Diploidy
Leaves
Life Sciences
Medicinal plant
Medicinal plants
Pharmaceutical industry
Plant Breeding/Biotechnology
Plant Genetics and Genomics
Plant Sciences
Plantlets
Plants
Polyploidy
Research Reports
Rhizomes
Seeds
Stomata
Surface water
Tetraploid
Tetraploidy
title In vitro induction and identification of autotetraploids of Dioscorea zingiberensis
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