Expression of a cotton MADS-box gene is regulated in anther development and in response to phytohormone signaling
MADS-box gene family encodes a large number and variety of transcription regulators in plants. In this study, a cDNA, GhMADS9, encoding a typical MADS protein with 230 amino acids was isolated from cotton flower cDNA library. Subsequently, a 1,623 bp genomic DNA fragment of GhMADS9 gene was isolated...
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| Veröffentlicht in: | Journal of genetics and genomics 2010-12, Vol.37 (12), p.805-816 |
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| creator | Shao, Su-Qiang Li, Bing-Ying Zhang, Ze-Ting Zhou, Ying Jiang, Jia Li, Xue-Bao |
| description | MADS-box gene family encodes a large number and variety of transcription regulators in plants. In this study, a cDNA, GhMADS9, encoding a typical MADS protein with 230 amino acids was isolated from cotton flower cDNA library. Subsequently, a 1,623 bp genomic DNA fragment of GhMADS9 gene was isolated in cotton by PCR. Compared with its cDNA sequence, six introns were found in GhMADS9 gene. Fluorescent microscopy indicated that GhMADS9 protein localized in the nucleus. Transactivation activity assay in yeast cells revealed that GhMADS9 protein did not show transcriptional activation. Quantitative RT-PCR analysis showed that GhMADS9 was specially expressed in cotton anthers. Further in situ hybridization analysis demonstrated that strong expression of GhMADS9 gene was detected in developing pollens, but no or weak signals were found in the other anther tissues. Furthermore, GhMADS9 expression was dramatically up-regulated in anthers with abscisic acid （ABA） treatment, whereas its activity was down-regulated when treated by gibberellin （GA3）. Collectively, our results suggest that GhMADS9 is a transcription factor and might be involved in cotton anther/pollen development and in response to ABA and GA3 signaling. |
| doi_str_mv | 10.1016/S1673-8527(09)60098-9 |
| format | Article |
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In this study, a cDNA, GhMADS9, encoding a typical MADS protein with 230 amino acids was isolated from cotton flower cDNA library. Subsequently, a 1,623 bp genomic DNA fragment of GhMADS9 gene was isolated in cotton by PCR. Compared with its cDNA sequence, six introns were found in GhMADS9 gene. Fluorescent microscopy indicated that GhMADS9 protein localized in the nucleus. Transactivation activity assay in yeast cells revealed that GhMADS9 protein did not show transcriptional activation. Quantitative RT-PCR analysis showed that GhMADS9 was specially expressed in cotton anthers. Further in situ hybridization analysis demonstrated that strong expression of GhMADS9 gene was detected in developing pollens, but no or weak signals were found in the other anther tissues. Furthermore, GhMADS9 expression was dramatically up-regulated in anthers with abscisic acid （ABA） treatment, whereas its activity was down-regulated when treated by gibberellin （GA3）. Collectively, our results suggest that GhMADS9 is a transcription factor and might be involved in cotton anther/pollen development and in response to ABA and GA3 signaling.</description><identifier>ISSN: 1673-8527</identifier><identifier>DOI: 10.1016/S1673-8527(09)60098-9</identifier><identifier>PMID: 21193159</identifier><language>eng</language><publisher>China: Elsevier Ltd</publisher><subject>Amino Acid Sequence ; anther/pollen development ; Base Sequence ; cDNA文库 ; Cell Line, Transformed ; cotton ( Gossypium hirsutum) ; DNA, Plant - chemistry ; DNA, Plant - isolation & purification ; Flowers - growth & development ; Flowers - metabolism ; gene expression ; Gene Expression Regulation, Developmental ; Gene Expression Regulation, Plant ; Gene Library ; Genes, Plant ; Gossypium - cytology ; Gossypium - genetics ; Gossypium - growth & development ; Gossypium - metabolism ; MADS Domain Proteins - chemistry ; MADS Domain Proteins - genetics ; MADS protein ; MADS盒基因 ; Molecular Sequence Data ; PCR分析 ; Phylogeny ; phytohormone ; Plant Growth Regulators - metabolism ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - metabolism ; Signal Transduction ; Transcriptional Activation ; 信号响应 ; 棉花 ; 植物激素 ; 花药发育 ; 表达调控</subject><ispartof>Journal of genetics and genomics, 2010-12, Vol.37 (12), p.805-816</ispartof><rights>2010 Institute of Genetics and Developmental Biology and the Genetics Society of China</rights><rights>Copyright © 2010 Institute of Genetics and Developmental Biology and the Genetics Society of China. Published by Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-8902cd96e92482741f6db8be4d3170b190b7033057064574a6017bc6e1d83d9c3</citedby><cites>FETCH-LOGICAL-c423t-8902cd96e92482741f6db8be4d3170b190b7033057064574a6017bc6e1d83d9c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/95085X/95085X.jpg</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S1673-8527(09)60098-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27926,27927,45997</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21193159$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shao, Su-Qiang</creatorcontrib><creatorcontrib>Li, Bing-Ying</creatorcontrib><creatorcontrib>Zhang, Ze-Ting</creatorcontrib><creatorcontrib>Zhou, Ying</creatorcontrib><creatorcontrib>Jiang, Jia</creatorcontrib><creatorcontrib>Li, Xue-Bao</creatorcontrib><title>Expression of a cotton MADS-box gene is regulated in anther development and in response to phytohormone signaling</title><title>Journal of genetics and genomics</title><addtitle>Journal of Genetics and Genomics</addtitle><description>MADS-box gene family encodes a large number and variety of transcription regulators in plants. In this study, a cDNA, GhMADS9, encoding a typical MADS protein with 230 amino acids was isolated from cotton flower cDNA library. Subsequently, a 1,623 bp genomic DNA fragment of GhMADS9 gene was isolated in cotton by PCR. Compared with its cDNA sequence, six introns were found in GhMADS9 gene. Fluorescent microscopy indicated that GhMADS9 protein localized in the nucleus. Transactivation activity assay in yeast cells revealed that GhMADS9 protein did not show transcriptional activation. Quantitative RT-PCR analysis showed that GhMADS9 was specially expressed in cotton anthers. Further in situ hybridization analysis demonstrated that strong expression of GhMADS9 gene was detected in developing pollens, but no or weak signals were found in the other anther tissues. Furthermore, GhMADS9 expression was dramatically up-regulated in anthers with abscisic acid （ABA） treatment, whereas its activity was down-regulated when treated by gibberellin （GA3）. Collectively, our results suggest that GhMADS9 is a transcription factor and might be involved in cotton anther/pollen development and in response to ABA and GA3 signaling.</description><subject>Amino Acid Sequence</subject><subject>anther/pollen development</subject><subject>Base Sequence</subject><subject>cDNA文库</subject><subject>Cell Line, Transformed</subject><subject>cotton ( Gossypium hirsutum)</subject><subject>DNA, Plant - chemistry</subject><subject>DNA, Plant - isolation & purification</subject><subject>Flowers - growth & development</subject><subject>Flowers - metabolism</subject><subject>gene expression</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gene Library</subject><subject>Genes, Plant</subject><subject>Gossypium - cytology</subject><subject>Gossypium - genetics</subject><subject>Gossypium - growth & development</subject><subject>Gossypium - metabolism</subject><subject>MADS Domain Proteins - chemistry</subject><subject>MADS Domain Proteins - genetics</subject><subject>MADS protein</subject><subject>MADS盒基因</subject><subject>Molecular Sequence Data</subject><subject>PCR分析</subject><subject>Phylogeny</subject><subject>phytohormone</subject><subject>Plant Growth Regulators - metabolism</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Signal Transduction</subject><subject>Transcriptional Activation</subject><subject>信号响应</subject><subject>棉花</subject><subject>植物激素</subject><subject>花药发育</subject><subject>表达调控</subject><issn>1673-8527</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtP3TAQhbNoBYjyE1pZ3bRdBPxI7HhVIaCARNUF7dpy7Emu1cTOtX0R_Pv6PsqWla2ZM2dG36mqjwSfE0z4xSPhgtVdS8VXLL9xjGVXy3fVyWv5uDpLyfUYU8ykEOKoOqaESEZaeVKtb56XCKUfPAoD0siEnMv_5-X1Y92HZzSCB-QSijBuJp3BIueR9nkFEVl4giksM_hcSrtO8VqCT4ByQMvqJYdViHMoFsmNXk_Ojx-q94OeEpwd3tPqz4-b31d39cOv2_ury4faNJTlupOYGis5SNp0VDRk4LbvemgsIwL3ROJeYMZwKzBvWtFojonoDQdiO2alYafVl73vEsN6Aymr2SUD06Q9hE1Ssm0KLNHhN5UdpYyTlsmibPdKE0NKEQa1RDfr-KIIVts01C4NtcWusFS7NNR27tNhw6afwb5O_c-hCL7vBVCIPDmIKhkH3oB1EUxWNrg3V3w-nLYKflwX0KrX5u_gJlCM0wKqY-wfOQumtg</recordid><startdate>20101201</startdate><enddate>20101201</enddate><creator>Shao, Su-Qiang</creator><creator>Li, Bing-Ying</creator><creator>Zhang, Ze-Ting</creator><creator>Zhou, Ying</creator><creator>Jiang, Jia</creator><creator>Li, Xue-Bao</creator><general>Elsevier Ltd</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>WU4</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20101201</creationdate><title>Expression of a cotton MADS-box gene is regulated in anther development and in response to phytohormone signaling</title><author>Shao, Su-Qiang ; Li, Bing-Ying ; Zhang, Ze-Ting ; Zhou, Ying ; Jiang, Jia ; Li, Xue-Bao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-8902cd96e92482741f6db8be4d3170b190b7033057064574a6017bc6e1d83d9c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Amino Acid Sequence</topic><topic>anther/pollen development</topic><topic>Base Sequence</topic><topic>cDNA文库</topic><topic>Cell Line, Transformed</topic><topic>cotton ( Gossypium hirsutum)</topic><topic>DNA, Plant - chemistry</topic><topic>DNA, Plant - isolation & purification</topic><topic>Flowers - growth & development</topic><topic>Flowers - metabolism</topic><topic>gene expression</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Gene Expression Regulation, Plant</topic><topic>Gene Library</topic><topic>Genes, Plant</topic><topic>Gossypium - cytology</topic><topic>Gossypium - genetics</topic><topic>Gossypium - growth & development</topic><topic>Gossypium - metabolism</topic><topic>MADS Domain Proteins - chemistry</topic><topic>MADS Domain Proteins - genetics</topic><topic>MADS protein</topic><topic>MADS盒基因</topic><topic>Molecular Sequence Data</topic><topic>PCR分析</topic><topic>Phylogeny</topic><topic>phytohormone</topic><topic>Plant Growth Regulators - metabolism</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Signal Transduction</topic><topic>Transcriptional Activation</topic><topic>信号响应</topic><topic>棉花</topic><topic>植物激素</topic><topic>花药发育</topic><topic>表达调控</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shao, Su-Qiang</creatorcontrib><creatorcontrib>Li, Bing-Ying</creatorcontrib><creatorcontrib>Zhang, Ze-Ting</creatorcontrib><creatorcontrib>Zhou, Ying</creatorcontrib><creatorcontrib>Jiang, Jia</creatorcontrib><creatorcontrib>Li, Xue-Bao</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库-自然科学-生物科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of genetics and genomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shao, Su-Qiang</au><au>Li, Bing-Ying</au><au>Zhang, Ze-Ting</au><au>Zhou, Ying</au><au>Jiang, Jia</au><au>Li, Xue-Bao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of a cotton MADS-box gene is regulated in anther development and in response to phytohormone signaling</atitle><jtitle>Journal of genetics and genomics</jtitle><addtitle>Journal of Genetics and Genomics</addtitle><date>2010-12-01</date><risdate>2010</risdate><volume>37</volume><issue>12</issue><spage>805</spage><epage>816</epage><pages>805-816</pages><issn>1673-8527</issn><abstract>MADS-box gene family encodes a large number and variety of transcription regulators in plants. In this study, a cDNA, GhMADS9, encoding a typical MADS protein with 230 amino acids was isolated from cotton flower cDNA library. Subsequently, a 1,623 bp genomic DNA fragment of GhMADS9 gene was isolated in cotton by PCR. Compared with its cDNA sequence, six introns were found in GhMADS9 gene. Fluorescent microscopy indicated that GhMADS9 protein localized in the nucleus. Transactivation activity assay in yeast cells revealed that GhMADS9 protein did not show transcriptional activation. Quantitative RT-PCR analysis showed that GhMADS9 was specially expressed in cotton anthers. Further in situ hybridization analysis demonstrated that strong expression of GhMADS9 gene was detected in developing pollens, but no or weak signals were found in the other anther tissues. Furthermore, GhMADS9 expression was dramatically up-regulated in anthers with abscisic acid （ABA） treatment, whereas its activity was down-regulated when treated by gibberellin （GA3）. Collectively, our results suggest that GhMADS9 is a transcription factor and might be involved in cotton anther/pollen development and in response to ABA and GA3 signaling.</abstract><cop>China</cop><pub>Elsevier Ltd</pub><pmid>21193159</pmid><doi>10.1016/S1673-8527(09)60098-9</doi><tpages>12</tpages></addata></record> |
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| subjects | Amino Acid Sequence anther/pollen development Base Sequence cDNA文库 Cell Line, Transformed cotton ( Gossypium hirsutum) DNA, Plant - chemistry DNA, Plant - isolation & purification Flowers - growth & development Flowers - metabolism gene expression Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Gene Library Genes, Plant Gossypium - cytology Gossypium - genetics Gossypium - growth & development Gossypium - metabolism MADS Domain Proteins - chemistry MADS Domain Proteins - genetics MADS protein MADS盒基因 Molecular Sequence Data PCR分析 Phylogeny phytohormone Plant Growth Regulators - metabolism Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Signal Transduction Transcriptional Activation 信号响应 棉花 植物激素 花药发育 表达调控 |
| title | Expression of a cotton MADS-box gene is regulated in anther development and in response to phytohormone signaling |
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