In situ expression of ribosomal protein L21 in developing tooth germ of the mouse lower first molar
We previously performed cDNA subtraction between the mouse mandibles at embryonic day 10.5 (E10.5) in the pre-initiation stage of the odontogenesis and E12.0 in the late initiation stage to investigate the key regulator genes in odontogenesis. Ribosomal protein L21 (Rpl21) is one of differentially e...
Gespeichert in:
| Veröffentlicht in: | Journal of molecular histology 2009-10, Vol.40 (5-6), p.361-367 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 367 |
|---|---|
| container_issue | 5-6 |
| container_start_page | 361 |
| container_title | Journal of molecular histology |
| container_volume | 40 |
| creator | Xie, Ming Kobayashi, Ieyoshi Kiyoshima, Tamotsu Nagata, Kengo Ookuma, Yukiko Fujiwara, Hiroaki Sakai, Hidetaka |
| description | We previously performed cDNA subtraction between the mouse mandibles at embryonic day 10.5 (E10.5) in the pre-initiation stage of the odontogenesis and E12.0 in the late initiation stage to investigate the key regulator genes in odontogenesis. Ribosomal protein L21 (Rpl21) is one of differentially expressed genes in the E12.0 mandible. This study examined the precise expression pattern of Rpl21 mRNA in the mouse mandibular first molar by in situ hybridization. Rpl21 mRNA was expressed in the presumptive dental epithelium and the underlying mesenchyme at E10.5, and in the thickened dental epithelium at E12.0. Strong in situ signals were observed in the epithelial bud at E14.0, and in the enamel organ at E15.0. However, either no (E14.0) or only a weak (E15.0) in situ signal was found in the primary enamel knot at these gestational days. Rpl21 was strongly expressed in the inner enamel epithelium, cervical loop and dental lamina from E16.0 to E18.0. In addition, Rpl21 mRNA was also demonstrated in various developing cranio-facial organs. These results suggest that Rpl21 participates in the synthesis of various polypeptides which might be related to the initiation and the development of such tooth germ, and also in the synthesis of enamel components in the presecretory stage of the ameloblast. Rpl21 for protein synthesis might also be related to the morphogenesis of the developing cranio-facial organs. |
| doi_str_mv | 10.1007/s10735-009-9249-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_954600394</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1978986031</sourcerecordid><originalsourceid>FETCH-LOGICAL-c492t-b597d5ab88ff2a1b2fbeb7ccd8be7053c8d4864bcc274189c2fe8782e2c934eb3</originalsourceid><addsrcrecordid>eNqFkU9v1DAQxS0EomXhA3ABiwuntPbYWdtHVPGn0ko90J4t25lsXSXxYicFvj1epRSJA5zG8vzezLMfIa85O-OMqfPCmRJtw5hpDEjTqCfklLdb1YDQ6unjWZkT8qKUO8ZAb6V5Tk6AMSlAtKckXE60xHmh-OOQsZSYJpp6mqNPJY1uoIecZowT3QGntXR4j0M6xGlP55TmW7rHPB4V8y3SMS0F6ZC-Y6Z9zGWuN4PLL8mz3g0FXz3UDbn59PH64kuzu_p8efFh1wRpYG58a1TXOq9134PjHnqPXoXQaY-KtSLoTlb_PgRQkmsToEetNCAEIyR6sSHv17nV87cFy2zHWAIOg5uwOrOmlVvGhJH_JZUQHKCClXz3F3mXljzVZ1iAlkvDFa8QX6GQUykZe3vIcXT5p-XMHpOya1K2JmWPSdUFG_LmYfDiR-weFb-jqQCsQKmtqX7zn83_mvp2FfUuWbfPsdibr8C4YFxzqQQTvwAI0qeP</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>225149171</pqid></control><display><type>article</type><title>In situ expression of ribosomal protein L21 in developing tooth germ of the mouse lower first molar</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Xie, Ming ; Kobayashi, Ieyoshi ; Kiyoshima, Tamotsu ; Nagata, Kengo ; Ookuma, Yukiko ; Fujiwara, Hiroaki ; Sakai, Hidetaka</creator><creatorcontrib>Xie, Ming ; Kobayashi, Ieyoshi ; Kiyoshima, Tamotsu ; Nagata, Kengo ; Ookuma, Yukiko ; Fujiwara, Hiroaki ; Sakai, Hidetaka</creatorcontrib><description>We previously performed cDNA subtraction between the mouse mandibles at embryonic day 10.5 (E10.5) in the pre-initiation stage of the odontogenesis and E12.0 in the late initiation stage to investigate the key regulator genes in odontogenesis. Ribosomal protein L21 (Rpl21) is one of differentially expressed genes in the E12.0 mandible. This study examined the precise expression pattern of Rpl21 mRNA in the mouse mandibular first molar by in situ hybridization. Rpl21 mRNA was expressed in the presumptive dental epithelium and the underlying mesenchyme at E10.5, and in the thickened dental epithelium at E12.0. Strong in situ signals were observed in the epithelial bud at E14.0, and in the enamel organ at E15.0. However, either no (E14.0) or only a weak (E15.0) in situ signal was found in the primary enamel knot at these gestational days. Rpl21 was strongly expressed in the inner enamel epithelium, cervical loop and dental lamina from E16.0 to E18.0. In addition, Rpl21 mRNA was also demonstrated in various developing cranio-facial organs. These results suggest that Rpl21 participates in the synthesis of various polypeptides which might be related to the initiation and the development of such tooth germ, and also in the synthesis of enamel components in the presecretory stage of the ameloblast. Rpl21 for protein synthesis might also be related to the morphogenesis of the developing cranio-facial organs.</description><identifier>ISSN: 1567-2379</identifier><identifier>EISSN: 1567-2387</identifier><identifier>DOI: 10.1007/s10735-009-9249-7</identifier><identifier>PMID: 20043235</identifier><language>eng</language><publisher>Dordrecht: Dordrecht : Springer Netherlands</publisher><subject>Animals ; Biomedical and Life Sciences ; Biomedicine ; Cell Biology ; Developmental Biology ; Female ; Gene Expression Regulation, Developmental ; In Situ Hybridization ; Life Sciences ; Male ; Mice ; Mice, Inbred BALB C ; Molar - cytology ; Molar - embryology ; Molar - metabolism ; Organ Specificity ; Original Paper ; Ribosomal Proteins - genetics ; Ribosomal Proteins - metabolism ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Tooth Germ - cytology ; Tooth Germ - embryology ; Tooth Germ - metabolism</subject><ispartof>Journal of molecular histology, 2009-10, Vol.40 (5-6), p.361-367</ispartof><rights>Springer Science+Business Media B.V. 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c492t-b597d5ab88ff2a1b2fbeb7ccd8be7053c8d4864bcc274189c2fe8782e2c934eb3</citedby><cites>FETCH-LOGICAL-c492t-b597d5ab88ff2a1b2fbeb7ccd8be7053c8d4864bcc274189c2fe8782e2c934eb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10735-009-9249-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10735-009-9249-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,41493,42562,51324</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20043235$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xie, Ming</creatorcontrib><creatorcontrib>Kobayashi, Ieyoshi</creatorcontrib><creatorcontrib>Kiyoshima, Tamotsu</creatorcontrib><creatorcontrib>Nagata, Kengo</creatorcontrib><creatorcontrib>Ookuma, Yukiko</creatorcontrib><creatorcontrib>Fujiwara, Hiroaki</creatorcontrib><creatorcontrib>Sakai, Hidetaka</creatorcontrib><title>In situ expression of ribosomal protein L21 in developing tooth germ of the mouse lower first molar</title><title>Journal of molecular histology</title><addtitle>J Mol Hist</addtitle><addtitle>J Mol Histol</addtitle><description>We previously performed cDNA subtraction between the mouse mandibles at embryonic day 10.5 (E10.5) in the pre-initiation stage of the odontogenesis and E12.0 in the late initiation stage to investigate the key regulator genes in odontogenesis. Ribosomal protein L21 (Rpl21) is one of differentially expressed genes in the E12.0 mandible. This study examined the precise expression pattern of Rpl21 mRNA in the mouse mandibular first molar by in situ hybridization. Rpl21 mRNA was expressed in the presumptive dental epithelium and the underlying mesenchyme at E10.5, and in the thickened dental epithelium at E12.0. Strong in situ signals were observed in the epithelial bud at E14.0, and in the enamel organ at E15.0. However, either no (E14.0) or only a weak (E15.0) in situ signal was found in the primary enamel knot at these gestational days. Rpl21 was strongly expressed in the inner enamel epithelium, cervical loop and dental lamina from E16.0 to E18.0. In addition, Rpl21 mRNA was also demonstrated in various developing cranio-facial organs. These results suggest that Rpl21 participates in the synthesis of various polypeptides which might be related to the initiation and the development of such tooth germ, and also in the synthesis of enamel components in the presecretory stage of the ameloblast. Rpl21 for protein synthesis might also be related to the morphogenesis of the developing cranio-facial organs.</description><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Biology</subject><subject>Developmental Biology</subject><subject>Female</subject><subject>Gene Expression Regulation, Developmental</subject><subject>In Situ Hybridization</subject><subject>Life Sciences</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Molar - cytology</subject><subject>Molar - embryology</subject><subject>Molar - metabolism</subject><subject>Organ Specificity</subject><subject>Original Paper</subject><subject>Ribosomal Proteins - genetics</subject><subject>Ribosomal Proteins - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Tooth Germ - cytology</subject><subject>Tooth Germ - embryology</subject><subject>Tooth Germ - metabolism</subject><issn>1567-2379</issn><issn>1567-2387</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkU9v1DAQxS0EomXhA3ABiwuntPbYWdtHVPGn0ko90J4t25lsXSXxYicFvj1epRSJA5zG8vzezLMfIa85O-OMqfPCmRJtw5hpDEjTqCfklLdb1YDQ6unjWZkT8qKUO8ZAb6V5Tk6AMSlAtKckXE60xHmh-OOQsZSYJpp6mqNPJY1uoIecZowT3QGntXR4j0M6xGlP55TmW7rHPB4V8y3SMS0F6ZC-Y6Z9zGWuN4PLL8mz3g0FXz3UDbn59PH64kuzu_p8efFh1wRpYG58a1TXOq9134PjHnqPXoXQaY-KtSLoTlb_PgRQkmsToEetNCAEIyR6sSHv17nV87cFy2zHWAIOg5uwOrOmlVvGhJH_JZUQHKCClXz3F3mXljzVZ1iAlkvDFa8QX6GQUykZe3vIcXT5p-XMHpOya1K2JmWPSdUFG_LmYfDiR-weFb-jqQCsQKmtqX7zn83_mvp2FfUuWbfPsdibr8C4YFxzqQQTvwAI0qeP</recordid><startdate>20091001</startdate><enddate>20091001</enddate><creator>Xie, Ming</creator><creator>Kobayashi, Ieyoshi</creator><creator>Kiyoshima, Tamotsu</creator><creator>Nagata, Kengo</creator><creator>Ookuma, Yukiko</creator><creator>Fujiwara, Hiroaki</creator><creator>Sakai, Hidetaka</creator><general>Dordrecht : Springer Netherlands</general><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20091001</creationdate><title>In situ expression of ribosomal protein L21 in developing tooth germ of the mouse lower first molar</title><author>Xie, Ming ; Kobayashi, Ieyoshi ; Kiyoshima, Tamotsu ; Nagata, Kengo ; Ookuma, Yukiko ; Fujiwara, Hiroaki ; Sakai, Hidetaka</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c492t-b597d5ab88ff2a1b2fbeb7ccd8be7053c8d4864bcc274189c2fe8782e2c934eb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cell Biology</topic><topic>Developmental Biology</topic><topic>Female</topic><topic>Gene Expression Regulation, Developmental</topic><topic>In Situ Hybridization</topic><topic>Life Sciences</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Molar - cytology</topic><topic>Molar - embryology</topic><topic>Molar - metabolism</topic><topic>Organ Specificity</topic><topic>Original Paper</topic><topic>Ribosomal Proteins - genetics</topic><topic>Ribosomal Proteins - metabolism</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Tooth Germ - cytology</topic><topic>Tooth Germ - embryology</topic><topic>Tooth Germ - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xie, Ming</creatorcontrib><creatorcontrib>Kobayashi, Ieyoshi</creatorcontrib><creatorcontrib>Kiyoshima, Tamotsu</creatorcontrib><creatorcontrib>Nagata, Kengo</creatorcontrib><creatorcontrib>Ookuma, Yukiko</creatorcontrib><creatorcontrib>Fujiwara, Hiroaki</creatorcontrib><creatorcontrib>Sakai, Hidetaka</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of molecular histology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xie, Ming</au><au>Kobayashi, Ieyoshi</au><au>Kiyoshima, Tamotsu</au><au>Nagata, Kengo</au><au>Ookuma, Yukiko</au><au>Fujiwara, Hiroaki</au><au>Sakai, Hidetaka</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In situ expression of ribosomal protein L21 in developing tooth germ of the mouse lower first molar</atitle><jtitle>Journal of molecular histology</jtitle><stitle>J Mol Hist</stitle><addtitle>J Mol Histol</addtitle><date>2009-10-01</date><risdate>2009</risdate><volume>40</volume><issue>5-6</issue><spage>361</spage><epage>367</epage><pages>361-367</pages><issn>1567-2379</issn><eissn>1567-2387</eissn><abstract>We previously performed cDNA subtraction between the mouse mandibles at embryonic day 10.5 (E10.5) in the pre-initiation stage of the odontogenesis and E12.0 in the late initiation stage to investigate the key regulator genes in odontogenesis. Ribosomal protein L21 (Rpl21) is one of differentially expressed genes in the E12.0 mandible. This study examined the precise expression pattern of Rpl21 mRNA in the mouse mandibular first molar by in situ hybridization. Rpl21 mRNA was expressed in the presumptive dental epithelium and the underlying mesenchyme at E10.5, and in the thickened dental epithelium at E12.0. Strong in situ signals were observed in the epithelial bud at E14.0, and in the enamel organ at E15.0. However, either no (E14.0) or only a weak (E15.0) in situ signal was found in the primary enamel knot at these gestational days. Rpl21 was strongly expressed in the inner enamel epithelium, cervical loop and dental lamina from E16.0 to E18.0. In addition, Rpl21 mRNA was also demonstrated in various developing cranio-facial organs. These results suggest that Rpl21 participates in the synthesis of various polypeptides which might be related to the initiation and the development of such tooth germ, and also in the synthesis of enamel components in the presecretory stage of the ameloblast. Rpl21 for protein synthesis might also be related to the morphogenesis of the developing cranio-facial organs.</abstract><cop>Dordrecht</cop><pub>Dordrecht : Springer Netherlands</pub><pmid>20043235</pmid><doi>10.1007/s10735-009-9249-7</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1567-2379 |
| ispartof | Journal of molecular histology, 2009-10, Vol.40 (5-6), p.361-367 |
| issn | 1567-2379 1567-2387 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_954600394 |
| source | MEDLINE; SpringerNature Journals |
| subjects | Animals Biomedical and Life Sciences Biomedicine Cell Biology Developmental Biology Female Gene Expression Regulation, Developmental In Situ Hybridization Life Sciences Male Mice Mice, Inbred BALB C Molar - cytology Molar - embryology Molar - metabolism Organ Specificity Original Paper Ribosomal Proteins - genetics Ribosomal Proteins - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism Tooth Germ - cytology Tooth Germ - embryology Tooth Germ - metabolism |
| title | In situ expression of ribosomal protein L21 in developing tooth germ of the mouse lower first molar |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-16T02%3A55%3A37IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=In%20situ%20expression%20of%20ribosomal%20protein%20L21%20in%20developing%20tooth%20germ%20of%20the%20mouse%20lower%20first%20molar&rft.jtitle=Journal%20of%20molecular%20histology&rft.au=Xie,%20Ming&rft.date=2009-10-01&rft.volume=40&rft.issue=5-6&rft.spage=361&rft.epage=367&rft.pages=361-367&rft.issn=1567-2379&rft.eissn=1567-2387&rft_id=info:doi/10.1007/s10735-009-9249-7&rft_dat=%3Cproquest_cross%3E1978986031%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=225149171&rft_id=info:pmid/20043235&rfr_iscdi=true |