In vitro study of biofilm formation and effectiveness of antimicrobial treatment on various dental material surfaces

Summary Elevated proportions of Candida albicans in biofilms formed on dentures are associated with stomatitis whereas Streptococcus mutans accumulation on restorative materials can cause secondary caries. Candida albicans, S. mutans, saliva‐derived and C. albicans/saliva‐derived mixed biofilms were...

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Veröffentlicht in:Molecular oral microbiology 2010-12, Vol.25 (6), p.384-390
Hauptverfasser: Li, L., Finnegan, M.B., Özkan, S., Kim, Y., Lillehoj, P.B., Ho, C.-M., Lux, R., Mito, R., Loewy, Z., Shi, W.
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container_end_page 390
container_issue 6
container_start_page 384
container_title Molecular oral microbiology
container_volume 25
creator Li, L.
Finnegan, M.B.
Özkan, S.
Kim, Y.
Lillehoj, P.B.
Ho, C.-M.
Lux, R.
Mito, R.
Loewy, Z.
Shi, W.
description Summary Elevated proportions of Candida albicans in biofilms formed on dentures are associated with stomatitis whereas Streptococcus mutans accumulation on restorative materials can cause secondary caries. Candida albicans, S. mutans, saliva‐derived and C. albicans/saliva‐derived mixed biofilms were grown on different materials including acrylic denture, porcelain, hydroxyapatite (HA), and polystyrene. The resulting biomass was analysed by three‐dimensional image quantification and assessment of colony‐forming units. The efficacy of biofilm treatment with a dissolved denture cleansing tablet (Polident®) was also evaluated by colony counting. Biofilms formed on HA exhibited the most striking differences in biomass accumulation: biofilms comprising salivary bacteria accrued the highest total biomass whereas C. albicans biofilm formation was greatly reduced on the HA surface compared with other materials, including the acrylic denture surface. These results substantiate clinical findings that acrylic dentures can comprise a reservoir for C. albicans, which renders patients more susceptible to C. albicans infections and stomatitis. Additionally, treatment efficacy of the same type of biofilms varied significantly depending on the surface. Although single‐species biofilms formed on polystyrene surfaces exhibited the highest susceptibility to the treatment, the most surviving cells were recovered from HA surfaces for all types of biofilms tested. This study demonstrates that the nature of a surface influences biofilm characteristics including biomass accumulation and susceptibility to antimicrobial treatments. Such treatments should therefore be evaluated on the surfaces colonized by the target pathogen(s).
doi_str_mv 10.1111/j.2041-1014.2010.00586.x
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Candida albicans, S. mutans, saliva‐derived and C. albicans/saliva‐derived mixed biofilms were grown on different materials including acrylic denture, porcelain, hydroxyapatite (HA), and polystyrene. The resulting biomass was analysed by three‐dimensional image quantification and assessment of colony‐forming units. The efficacy of biofilm treatment with a dissolved denture cleansing tablet (Polident®) was also evaluated by colony counting. Biofilms formed on HA exhibited the most striking differences in biomass accumulation: biofilms comprising salivary bacteria accrued the highest total biomass whereas C. albicans biofilm formation was greatly reduced on the HA surface compared with other materials, including the acrylic denture surface. These results substantiate clinical findings that acrylic dentures can comprise a reservoir for C. albicans, which renders patients more susceptible to C. albicans infections and stomatitis. Additionally, treatment efficacy of the same type of biofilms varied significantly depending on the surface. Although single‐species biofilms formed on polystyrene surfaces exhibited the highest susceptibility to the treatment, the most surviving cells were recovered from HA surfaces for all types of biofilms tested. This study demonstrates that the nature of a surface influences biofilm characteristics including biomass accumulation and susceptibility to antimicrobial treatments. 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Candida albicans, S. mutans, saliva‐derived and C. albicans/saliva‐derived mixed biofilms were grown on different materials including acrylic denture, porcelain, hydroxyapatite (HA), and polystyrene. The resulting biomass was analysed by three‐dimensional image quantification and assessment of colony‐forming units. The efficacy of biofilm treatment with a dissolved denture cleansing tablet (Polident®) was also evaluated by colony counting. Biofilms formed on HA exhibited the most striking differences in biomass accumulation: biofilms comprising salivary bacteria accrued the highest total biomass whereas C. albicans biofilm formation was greatly reduced on the HA surface compared with other materials, including the acrylic denture surface. These results substantiate clinical findings that acrylic dentures can comprise a reservoir for C. albicans, which renders patients more susceptible to C. albicans infections and stomatitis. Additionally, treatment efficacy of the same type of biofilms varied significantly depending on the surface. Although single‐species biofilms formed on polystyrene surfaces exhibited the highest susceptibility to the treatment, the most surviving cells were recovered from HA surfaces for all types of biofilms tested. This study demonstrates that the nature of a surface influences biofilm characteristics including biomass accumulation and susceptibility to antimicrobial treatments. Such treatments should therefore be evaluated on the surfaces colonized by the target pathogen(s).</description><subject>Acrylic Resins - chemistry</subject><subject>Anti-Infective Agents - pharmacology</subject><subject>Antimicrobial agents</subject><subject>Bacterial Adhesion - drug effects</subject><subject>Bacterial Load</subject><subject>Bacteriology</subject><subject>Biofilms</subject><subject>Biofilms - drug effects</subject><subject>Biofilms - growth &amp; development</subject><subject>Biological and medical sciences</subject><subject>Biomass</subject><subject>Borates - pharmacology</subject><subject>Candida albicans</subject><subject>Candida albicans - drug effects</subject><subject>Candida albicans - physiology</subject><subject>Colonies</subject><subject>Dental caries</subject><subject>Dental Materials - chemistry</subject><subject>Dental Plaque - microbiology</subject><subject>Dental Porcelain - chemistry</subject><subject>Dental restorative materials</subject><subject>Dentistry</subject><subject>Denture Bases - microbiology</subject><subject>Denture Cleansers - pharmacology</subject><subject>denture stomatitis</subject><subject>Dentures</subject><subject>Durapatite - chemistry</subject><subject>Enumeration</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Hydroxyapatite</subject><subject>Imaging, Three-Dimensional</subject><subject>Infection</subject><subject>Materials Testing</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbiology</subject><subject>Microscopy, Confocal</subject><subject>Miscellaneous</subject><subject>Mycology</subject><subject>polystyrene</subject><subject>Polystyrenes - chemistry</subject><subject>Saliva - microbiology</subject><subject>Stomatitis</subject><subject>Streptococcus mutans</subject><subject>Streptococcus mutans - drug effects</subject><subject>Streptococcus mutans - physiology</subject><subject>Sulfates - pharmacology</subject><subject>Surface Properties</subject><subject>Tablets</subject><issn>2041-1006</issn><issn>2041-1014</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhiMEolXpX0C-IE7Z2vFXInFBKyiVWsqhqEdr4tiSl3wU21l2_z2T7rIcwReP7ecdz8xbFITRFcN1tVlVVLCSUSYwwltKZa1WuxfF-enh5Smm6qy4TGlDcXEmtNavi7OKUUElq86LfDOSbchxIinP3Z5MnrRh8qEfiJ_iADlMI4GxI857Z3PYutGltGAw5jAEG6c2QE9ydJAHN2aC_BZimOZEOjzjG2ZxcYHSHD1Yl94Urzz0yV0e94vi--dPD-sv5e399c36421pJeeqZC3TFpvRLdWqE1qwWgJneNVR3rSslRZoDVbSmupGaqkab52oGvDCOlbzi-L9Ie9TnH7OLmUzhGRd38PosD7TSCEbWVf0n6RWVVULzTiS9YHEzlOKzpunGAaIe8OoWfwxG7OM3iw2mMUf8-yP2aH07fGTuR1cdxL-cQOBd0cAkoXeRxhtSH85HIpSfKn2w4H7FXq3_-8CzN39HQYoLw_ykLLbneQQfxiluZbm8eu1EdXD4zfN14bz3wCyuhI</recordid><startdate>201012</startdate><enddate>201012</enddate><creator>Li, L.</creator><creator>Finnegan, M.B.</creator><creator>Özkan, S.</creator><creator>Kim, Y.</creator><creator>Lillehoj, P.B.</creator><creator>Ho, C.-M.</creator><creator>Lux, R.</creator><creator>Mito, R.</creator><creator>Loewy, Z.</creator><creator>Shi, W.</creator><general>Blackwell Publishing Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>201012</creationdate><title>In vitro study of biofilm formation and effectiveness of antimicrobial treatment on various dental material surfaces</title><author>Li, L. ; Finnegan, M.B. ; Özkan, S. ; Kim, Y. ; Lillehoj, P.B. ; Ho, C.-M. ; Lux, R. ; Mito, R. ; Loewy, Z. ; Shi, W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5336-1b17c0147b076d474185a31c01d039b1b5ca08ac50807957569fce429af4ce183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Acrylic Resins - chemistry</topic><topic>Anti-Infective Agents - pharmacology</topic><topic>Antimicrobial agents</topic><topic>Bacterial Adhesion - drug effects</topic><topic>Bacterial Load</topic><topic>Bacteriology</topic><topic>Biofilms</topic><topic>Biofilms - drug effects</topic><topic>Biofilms - growth &amp; development</topic><topic>Biological and medical sciences</topic><topic>Biomass</topic><topic>Borates - pharmacology</topic><topic>Candida albicans</topic><topic>Candida albicans - drug effects</topic><topic>Candida albicans - physiology</topic><topic>Colonies</topic><topic>Dental caries</topic><topic>Dental Materials - chemistry</topic><topic>Dental Plaque - microbiology</topic><topic>Dental Porcelain - chemistry</topic><topic>Dental restorative materials</topic><topic>Dentistry</topic><topic>Denture Bases - microbiology</topic><topic>Denture Cleansers - pharmacology</topic><topic>denture stomatitis</topic><topic>Dentures</topic><topic>Durapatite - chemistry</topic><topic>Enumeration</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Hydroxyapatite</topic><topic>Imaging, Three-Dimensional</topic><topic>Infection</topic><topic>Materials Testing</topic><topic>Microbial Sensitivity Tests</topic><topic>Microbiology</topic><topic>Microscopy, Confocal</topic><topic>Miscellaneous</topic><topic>Mycology</topic><topic>polystyrene</topic><topic>Polystyrenes - chemistry</topic><topic>Saliva - microbiology</topic><topic>Stomatitis</topic><topic>Streptococcus mutans</topic><topic>Streptococcus mutans - drug effects</topic><topic>Streptococcus mutans - physiology</topic><topic>Sulfates - pharmacology</topic><topic>Surface Properties</topic><topic>Tablets</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, L.</creatorcontrib><creatorcontrib>Finnegan, M.B.</creatorcontrib><creatorcontrib>Özkan, S.</creatorcontrib><creatorcontrib>Kim, Y.</creatorcontrib><creatorcontrib>Lillehoj, P.B.</creatorcontrib><creatorcontrib>Ho, C.-M.</creatorcontrib><creatorcontrib>Lux, R.</creatorcontrib><creatorcontrib>Mito, R.</creatorcontrib><creatorcontrib>Loewy, Z.</creatorcontrib><creatorcontrib>Shi, W.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Molecular oral microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, L.</au><au>Finnegan, M.B.</au><au>Özkan, S.</au><au>Kim, Y.</au><au>Lillehoj, P.B.</au><au>Ho, C.-M.</au><au>Lux, R.</au><au>Mito, R.</au><au>Loewy, Z.</au><au>Shi, W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro study of biofilm formation and effectiveness of antimicrobial treatment on various dental material surfaces</atitle><jtitle>Molecular oral microbiology</jtitle><addtitle>Mol Oral Microbiol</addtitle><date>2010-12</date><risdate>2010</risdate><volume>25</volume><issue>6</issue><spage>384</spage><epage>390</epage><pages>384-390</pages><issn>2041-1006</issn><eissn>2041-1014</eissn><abstract>Summary Elevated proportions of Candida albicans in biofilms formed on dentures are associated with stomatitis whereas Streptococcus mutans accumulation on restorative materials can cause secondary caries. Candida albicans, S. mutans, saliva‐derived and C. albicans/saliva‐derived mixed biofilms were grown on different materials including acrylic denture, porcelain, hydroxyapatite (HA), and polystyrene. The resulting biomass was analysed by three‐dimensional image quantification and assessment of colony‐forming units. The efficacy of biofilm treatment with a dissolved denture cleansing tablet (Polident®) was also evaluated by colony counting. Biofilms formed on HA exhibited the most striking differences in biomass accumulation: biofilms comprising salivary bacteria accrued the highest total biomass whereas C. albicans biofilm formation was greatly reduced on the HA surface compared with other materials, including the acrylic denture surface. These results substantiate clinical findings that acrylic dentures can comprise a reservoir for C. albicans, which renders patients more susceptible to C. albicans infections and stomatitis. Additionally, treatment efficacy of the same type of biofilms varied significantly depending on the surface. Although single‐species biofilms formed on polystyrene surfaces exhibited the highest susceptibility to the treatment, the most surviving cells were recovered from HA surfaces for all types of biofilms tested. This study demonstrates that the nature of a surface influences biofilm characteristics including biomass accumulation and susceptibility to antimicrobial treatments. Such treatments should therefore be evaluated on the surfaces colonized by the target pathogen(s).</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>21040512</pmid><doi>10.1111/j.2041-1014.2010.00586.x</doi><tpages>7</tpages></addata></record>
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subjects Acrylic Resins - chemistry
Anti-Infective Agents - pharmacology
Antimicrobial agents
Bacterial Adhesion - drug effects
Bacterial Load
Bacteriology
Biofilms
Biofilms - drug effects
Biofilms - growth & development
Biological and medical sciences
Biomass
Borates - pharmacology
Candida albicans
Candida albicans - drug effects
Candida albicans - physiology
Colonies
Dental caries
Dental Materials - chemistry
Dental Plaque - microbiology
Dental Porcelain - chemistry
Dental restorative materials
Dentistry
Denture Bases - microbiology
Denture Cleansers - pharmacology
denture stomatitis
Dentures
Durapatite - chemistry
Enumeration
Fundamental and applied biological sciences. Psychology
Humans
Hydroxyapatite
Imaging, Three-Dimensional
Infection
Materials Testing
Microbial Sensitivity Tests
Microbiology
Microscopy, Confocal
Miscellaneous
Mycology
polystyrene
Polystyrenes - chemistry
Saliva - microbiology
Stomatitis
Streptococcus mutans
Streptococcus mutans - drug effects
Streptococcus mutans - physiology
Sulfates - pharmacology
Surface Properties
Tablets
title In vitro study of biofilm formation and effectiveness of antimicrobial treatment on various dental material surfaces
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