An efficient particle bombardment system for the genetic transformation of asparagus (Asparagus officinalis L.)
The microprojectile bombardment method was used to transfer DNA into embryogenic callus of asparagus (Asparagus officcinalis L.) and to produce stably transformed asparagus plants. Embryogenic callus, derived from UC 157 and UC72 asparagus cultivars, was bombarded with tungsten particles coated with...
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| Veröffentlicht in: | Plant cell reports 1997-02, Vol.16 (5), p.255-260 |
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| creator | CABRERA-PONCE, J. L LOPEZ, L ASSAD-GARCIA, N MEDINA-AREVALO, C BAILEY, A. M HERRERA-ESTRELLA, L |
| description | The microprojectile bombardment method was used to transfer DNA into embryogenic callus of asparagus (Asparagus officcinalis L.) and to produce stably transformed asparagus plants. Embryogenic callus, derived from UC 157 and UC72 asparagus cultivars, was bombarded with tungsten particles coated with plasmid DNA that contained genes encoding hygromycin phosphotransferase, phosphinothricin acetyl transferase and β-glucuronidase. Putatively transformed calli were identified from the bombarded tissue after 4 months selection on 25 mg/L hygromycin B plus 4 mg/L phosphinothricin (PPT). By selecting embryogenic callus on hygromycin plus PPT the overall transformation and selection efficiencies were substantially improved over selection with hygromycin or PPT alone, where no transgenic clones were recovered. The transgenic nature of the selected material was demonstrated by GUS histochemical assays and Southern blot hybridization analysis. Transgenic asparagus plants were found to withstand the prescribed levels of the PPT-based herbicide BASTATM for weed control. |
| doi_str_mv | 10.1007/BF01088276 |
| format | Article |
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L ; LOPEZ, L ; ASSAD-GARCIA, N ; MEDINA-AREVALO, C ; BAILEY, A. M ; HERRERA-ESTRELLA, L</creator><creatorcontrib>CABRERA-PONCE, J. L ; LOPEZ, L ; ASSAD-GARCIA, N ; MEDINA-AREVALO, C ; BAILEY, A. M ; HERRERA-ESTRELLA, L</creatorcontrib><description>The microprojectile bombardment method was used to transfer DNA into embryogenic callus of asparagus (Asparagus officcinalis L.) and to produce stably transformed asparagus plants. Embryogenic callus, derived from UC 157 and UC72 asparagus cultivars, was bombarded with tungsten particles coated with plasmid DNA that contained genes encoding hygromycin phosphotransferase, phosphinothricin acetyl transferase and β-glucuronidase. Putatively transformed calli were identified from the bombarded tissue after 4 months selection on 25 mg/L hygromycin B plus 4 mg/L phosphinothricin (PPT). By selecting embryogenic callus on hygromycin plus PPT the overall transformation and selection efficiencies were substantially improved over selection with hygromycin or PPT alone, where no transgenic clones were recovered. The transgenic nature of the selected material was demonstrated by GUS histochemical assays and Southern blot hybridization analysis. Transgenic asparagus plants were found to withstand the prescribed levels of the PPT-based herbicide BASTATM for weed control.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/BF01088276</identifier><identifier>PMID: 30727658</identifier><identifier>CODEN: PCRPD8</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Asparagus ; Asparagus officinalis ; Biological and medical sciences ; Biotechnology ; Cultivars ; Deoxyribonucleic acid ; DNA ; Fundamental and applied biological sciences. Psychology ; Genetic engineering ; Genetic technics ; Methods. Procedures. Technologies ; Transgenic animals and transgenic plants ; Transgenic plants ; Tungsten ; Weed control ; Weeds</subject><ispartof>Plant cell reports, 1997-02, Vol.16 (5), p.255-260</ispartof><rights>1997 INIST-CNRS</rights><rights>Springer-Verlag 1997</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2928-7d368556dd38d2a95a75b7af4a71694925b1e7b16f4a494bae130c7cdd3476003</citedby><cites>FETCH-LOGICAL-c2928-7d368556dd38d2a95a75b7af4a71694925b1e7b16f4a494bae130c7cdd3476003</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2576490$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30727658$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>CABRERA-PONCE, J. L</creatorcontrib><creatorcontrib>LOPEZ, L</creatorcontrib><creatorcontrib>ASSAD-GARCIA, N</creatorcontrib><creatorcontrib>MEDINA-AREVALO, C</creatorcontrib><creatorcontrib>BAILEY, A. M</creatorcontrib><creatorcontrib>HERRERA-ESTRELLA, L</creatorcontrib><title>An efficient particle bombardment system for the genetic transformation of asparagus (Asparagus officinalis L.)</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><description>The microprojectile bombardment method was used to transfer DNA into embryogenic callus of asparagus (Asparagus officcinalis L.) and to produce stably transformed asparagus plants. Embryogenic callus, derived from UC 157 and UC72 asparagus cultivars, was bombarded with tungsten particles coated with plasmid DNA that contained genes encoding hygromycin phosphotransferase, phosphinothricin acetyl transferase and β-glucuronidase. Putatively transformed calli were identified from the bombarded tissue after 4 months selection on 25 mg/L hygromycin B plus 4 mg/L phosphinothricin (PPT). By selecting embryogenic callus on hygromycin plus PPT the overall transformation and selection efficiencies were substantially improved over selection with hygromycin or PPT alone, where no transgenic clones were recovered. The transgenic nature of the selected material was demonstrated by GUS histochemical assays and Southern blot hybridization analysis. Transgenic asparagus plants were found to withstand the prescribed levels of the PPT-based herbicide BASTATM for weed control.</description><subject>Asparagus</subject><subject>Asparagus officinalis</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cultivars</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Methods. Procedures. 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L ; LOPEZ, L ; ASSAD-GARCIA, N ; MEDINA-AREVALO, C ; BAILEY, A. M ; HERRERA-ESTRELLA, L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2928-7d368556dd38d2a95a75b7af4a71694925b1e7b16f4a494bae130c7cdd3476003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Asparagus</topic><topic>Asparagus officinalis</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cultivars</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Methods. Procedures. 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L</au><au>LOPEZ, L</au><au>ASSAD-GARCIA, N</au><au>MEDINA-AREVALO, C</au><au>BAILEY, A. M</au><au>HERRERA-ESTRELLA, L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An efficient particle bombardment system for the genetic transformation of asparagus (Asparagus officinalis L.)</atitle><jtitle>Plant cell reports</jtitle><addtitle>Plant Cell Rep</addtitle><date>1997-02</date><risdate>1997</risdate><volume>16</volume><issue>5</issue><spage>255</spage><epage>260</epage><pages>255-260</pages><issn>0721-7714</issn><eissn>1432-203X</eissn><coden>PCRPD8</coden><abstract>The microprojectile bombardment method was used to transfer DNA into embryogenic callus of asparagus (Asparagus officcinalis L.) and to produce stably transformed asparagus plants. Embryogenic callus, derived from UC 157 and UC72 asparagus cultivars, was bombarded with tungsten particles coated with plasmid DNA that contained genes encoding hygromycin phosphotransferase, phosphinothricin acetyl transferase and β-glucuronidase. Putatively transformed calli were identified from the bombarded tissue after 4 months selection on 25 mg/L hygromycin B plus 4 mg/L phosphinothricin (PPT). By selecting embryogenic callus on hygromycin plus PPT the overall transformation and selection efficiencies were substantially improved over selection with hygromycin or PPT alone, where no transgenic clones were recovered. The transgenic nature of the selected material was demonstrated by GUS histochemical assays and Southern blot hybridization analysis. Transgenic asparagus plants were found to withstand the prescribed levels of the PPT-based herbicide BASTATM for weed control.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>30727658</pmid><doi>10.1007/BF01088276</doi><tpages>6</tpages></addata></record> |
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| ispartof | Plant cell reports, 1997-02, Vol.16 (5), p.255-260 |
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| language | eng |
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| source | SpringerLink Journals - AutoHoldings |
| subjects | Asparagus Asparagus officinalis Biological and medical sciences Biotechnology Cultivars Deoxyribonucleic acid DNA Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Methods. Procedures. Technologies Transgenic animals and transgenic plants Transgenic plants Tungsten Weed control Weeds |
| title | An efficient particle bombardment system for the genetic transformation of asparagus (Asparagus officinalis L.) |
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