Analysis of erythropoietin glycoform produced by recombinant CHO cells using the Lectin-blotting technique

The glycosylation pattern of Erythropoietin (EPO), produced by recombinant CHO cells, was studied using the simple and rapid technique of 'Lectin-blotting'. In this experiment we used three different kinds of lectins, MAA (Maackia amurensis agglutinine), RCA (Ricinus communis agglutinine),...

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Veröffentlicht in:	Biotechnology and bioprocess engineering 1998-06, Vol.3 (1), p.40-43
Hauptverfasser:	Chang, Kern Hee, Kim, Kyung Soo, Kim, Jung Hoe
Format:	Artikel
Sprache:	eng
Schlagworte:	Ammonium Ammonium chloride Carbohydrates Chains Chemical compounds Chloroquine Datura stramonium Erythropoietin Galactose Glutamine Glycosylation Lectins Metabolism N-Acetyllactosamine Ricinus communis Sialic acids
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