Species-specific detection of Bipolaris sorokiniana from wheat and barley tissues
Cochliobolus sativus (anamorph: Bipolaris sorokiniana) is a fungal pathogen that can cause yield limiting diseases of barley and wheat. A PCR-based diagnostic assay was developed to detect B. sorokiniana in barley and wheat tissues using the pathogen-specific primers COSA_F/R derived from the melani...
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Veröffentlicht in: | Crop protection 2010-11, Vol.29 (11), p.1325-1330 |
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creator | Matusinsky, P. Frei, P. Mikolasova, R. Svacinova, I. Tvaruzek, L. Spitzer, T. |
description | Cochliobolus sativus (anamorph:
Bipolaris sorokiniana) is a fungal pathogen that can cause yield limiting diseases of barley and wheat. A PCR-based diagnostic assay was developed to detect
B. sorokiniana in barley and wheat tissues using the pathogen-specific primers COSA_F/R derived from the melanin biosynthesis pathway
Brn1 locus. Specificity was tested against more than 30 fungal organisms associated with barley and wheat. Limit of detection was determined to be 0.001
ng for pure fungal DNA. The species-specific primers were capable of detecting the pathogen in planta, specifically from infected barley leaves and wheat roots and stem bases. |
doi_str_mv | 10.1016/j.cropro.2010.07.013 |
format | Article |
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Bipolaris sorokiniana) is a fungal pathogen that can cause yield limiting diseases of barley and wheat. A PCR-based diagnostic assay was developed to detect
B. sorokiniana in barley and wheat tissues using the pathogen-specific primers COSA_F/R derived from the melanin biosynthesis pathway
Brn1 locus. Specificity was tested against more than 30 fungal organisms associated with barley and wheat. Limit of detection was determined to be 0.001
ng for pure fungal DNA. The species-specific primers were capable of detecting the pathogen in planta, specifically from infected barley leaves and wheat roots and stem bases.</description><identifier>ISSN: 0261-2194</identifier><identifier>EISSN: 1873-6904</identifier><identifier>DOI: 10.1016/j.cropro.2010.07.013</identifier><language>eng</language><publisher>Elsevier Ltd</publisher><subject>barley ; biochemical pathways ; Biosynthesis ; Bipolaris sorokiniana ; Cereal diseases ; Cochliobolus sativus ; diagnostic techniques ; disease detection ; disease diagnosis ; Hordeum vulgare ; host plants ; leaves ; loci ; microbial genetics ; Molecular diagnostics ; plant pathogenic fungi ; plant tissues ; polymerase chain reaction ; roots ; Spot blotch ; stems ; Triticum aestivum ; wheat</subject><ispartof>Crop protection, 2010-11, Vol.29 (11), p.1325-1330</ispartof><rights>2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c428t-65bfd4efb0e996a4e1f3ccdd9be3af3705951607bf58b247b722382e8ea14f813</citedby><cites>FETCH-LOGICAL-c428t-65bfd4efb0e996a4e1f3ccdd9be3af3705951607bf58b247b722382e8ea14f813</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.cropro.2010.07.013$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids></links><search><creatorcontrib>Matusinsky, P.</creatorcontrib><creatorcontrib>Frei, P.</creatorcontrib><creatorcontrib>Mikolasova, R.</creatorcontrib><creatorcontrib>Svacinova, I.</creatorcontrib><creatorcontrib>Tvaruzek, L.</creatorcontrib><creatorcontrib>Spitzer, T.</creatorcontrib><title>Species-specific detection of Bipolaris sorokiniana from wheat and barley tissues</title><title>Crop protection</title><description>Cochliobolus sativus (anamorph:
Bipolaris sorokiniana) is a fungal pathogen that can cause yield limiting diseases of barley and wheat. A PCR-based diagnostic assay was developed to detect
B. sorokiniana in barley and wheat tissues using the pathogen-specific primers COSA_F/R derived from the melanin biosynthesis pathway
Brn1 locus. Specificity was tested against more than 30 fungal organisms associated with barley and wheat. Limit of detection was determined to be 0.001
ng for pure fungal DNA. The species-specific primers were capable of detecting the pathogen in planta, specifically from infected barley leaves and wheat roots and stem bases.</description><subject>barley</subject><subject>biochemical pathways</subject><subject>Biosynthesis</subject><subject>Bipolaris sorokiniana</subject><subject>Cereal diseases</subject><subject>Cochliobolus sativus</subject><subject>diagnostic techniques</subject><subject>disease detection</subject><subject>disease diagnosis</subject><subject>Hordeum vulgare</subject><subject>host plants</subject><subject>leaves</subject><subject>loci</subject><subject>microbial genetics</subject><subject>Molecular diagnostics</subject><subject>plant pathogenic fungi</subject><subject>plant tissues</subject><subject>polymerase chain reaction</subject><subject>roots</subject><subject>Spot blotch</subject><subject>stems</subject><subject>Triticum aestivum</subject><subject>wheat</subject><issn>0261-2194</issn><issn>1873-6904</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LAzEQhoMoWKv_QDA3T1vztbvJRVDxCwoiteeQzU40dbupyVbpvzdlPXsaGJ6Zl_dB6JySGSW0ulrNbAybGGaM5BWpZ4TyAzShsuZFpYg4RBPCKlowqsQxOklpRQhhnLMJel1swHpIRdpP5y1uYQA7-NDj4PCt34TORJ9wCjF8-t6b3mAXwxr_fIAZsOlb3JjYwQ4PPqUtpFN05EyX4OxvTtHy4f7t7qmYvzw-393MCyuYHIqqbFwrwDUElKqMAOq4tW2rGuDG8ZqUqqQVqRtXyoaJuqkZ45KBBEOFk5RP0eX4Nxf_yrmDXvtkoetMD2GbtCpFKVUleSbFSGZLKUVwehP92sSdpkTvBeqVHgXqvUBNap0F5rOL8cyZoM17lqCXiwxwQqVUlNWZuB4JyD2_PUSdssveQutjdqjb4P-P-AWcHIX-</recordid><startdate>20101101</startdate><enddate>20101101</enddate><creator>Matusinsky, P.</creator><creator>Frei, P.</creator><creator>Mikolasova, R.</creator><creator>Svacinova, I.</creator><creator>Tvaruzek, L.</creator><creator>Spitzer, T.</creator><general>Elsevier Ltd</general><general>[Amsterdam]: Elsevier Science</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>C1K</scope><scope>SOI</scope></search><sort><creationdate>20101101</creationdate><title>Species-specific detection of Bipolaris sorokiniana from wheat and barley tissues</title><author>Matusinsky, P. ; Frei, P. ; Mikolasova, R. ; Svacinova, I. ; Tvaruzek, L. ; Spitzer, T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c428t-65bfd4efb0e996a4e1f3ccdd9be3af3705951607bf58b247b722382e8ea14f813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>barley</topic><topic>biochemical pathways</topic><topic>Biosynthesis</topic><topic>Bipolaris sorokiniana</topic><topic>Cereal diseases</topic><topic>Cochliobolus sativus</topic><topic>diagnostic techniques</topic><topic>disease detection</topic><topic>disease diagnosis</topic><topic>Hordeum vulgare</topic><topic>host plants</topic><topic>leaves</topic><topic>loci</topic><topic>microbial genetics</topic><topic>Molecular diagnostics</topic><topic>plant pathogenic fungi</topic><topic>plant tissues</topic><topic>polymerase chain reaction</topic><topic>roots</topic><topic>Spot blotch</topic><topic>stems</topic><topic>Triticum aestivum</topic><topic>wheat</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Matusinsky, P.</creatorcontrib><creatorcontrib>Frei, P.</creatorcontrib><creatorcontrib>Mikolasova, R.</creatorcontrib><creatorcontrib>Svacinova, I.</creatorcontrib><creatorcontrib>Tvaruzek, L.</creatorcontrib><creatorcontrib>Spitzer, T.</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><jtitle>Crop protection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Matusinsky, P.</au><au>Frei, P.</au><au>Mikolasova, R.</au><au>Svacinova, I.</au><au>Tvaruzek, L.</au><au>Spitzer, T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Species-specific detection of Bipolaris sorokiniana from wheat and barley tissues</atitle><jtitle>Crop protection</jtitle><date>2010-11-01</date><risdate>2010</risdate><volume>29</volume><issue>11</issue><spage>1325</spage><epage>1330</epage><pages>1325-1330</pages><issn>0261-2194</issn><eissn>1873-6904</eissn><abstract>Cochliobolus sativus (anamorph:
Bipolaris sorokiniana) is a fungal pathogen that can cause yield limiting diseases of barley and wheat. A PCR-based diagnostic assay was developed to detect
B. sorokiniana in barley and wheat tissues using the pathogen-specific primers COSA_F/R derived from the melanin biosynthesis pathway
Brn1 locus. Specificity was tested against more than 30 fungal organisms associated with barley and wheat. Limit of detection was determined to be 0.001
ng for pure fungal DNA. The species-specific primers were capable of detecting the pathogen in planta, specifically from infected barley leaves and wheat roots and stem bases.</abstract><pub>Elsevier Ltd</pub><doi>10.1016/j.cropro.2010.07.013</doi><tpages>6</tpages></addata></record> |
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language | eng |
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source | Elsevier ScienceDirect Journals |
subjects | barley biochemical pathways Biosynthesis Bipolaris sorokiniana Cereal diseases Cochliobolus sativus diagnostic techniques disease detection disease diagnosis Hordeum vulgare host plants leaves loci microbial genetics Molecular diagnostics plant pathogenic fungi plant tissues polymerase chain reaction roots Spot blotch stems Triticum aestivum wheat |
title | Species-specific detection of Bipolaris sorokiniana from wheat and barley tissues |
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