Species-specific detection of Bipolaris sorokiniana from wheat and barley tissues

Cochliobolus sativus (anamorph: Bipolaris sorokiniana) is a fungal pathogen that can cause yield limiting diseases of barley and wheat. A PCR-based diagnostic assay was developed to detect B. sorokiniana in barley and wheat tissues using the pathogen-specific primers COSA_F/R derived from the melani...

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Veröffentlicht in:Crop protection 2010-11, Vol.29 (11), p.1325-1330
Hauptverfasser: Matusinsky, P., Frei, P., Mikolasova, R., Svacinova, I., Tvaruzek, L., Spitzer, T.
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container_end_page 1330
container_issue 11
container_start_page 1325
container_title Crop protection
container_volume 29
creator Matusinsky, P.
Frei, P.
Mikolasova, R.
Svacinova, I.
Tvaruzek, L.
Spitzer, T.
description Cochliobolus sativus (anamorph: Bipolaris sorokiniana) is a fungal pathogen that can cause yield limiting diseases of barley and wheat. A PCR-based diagnostic assay was developed to detect B. sorokiniana in barley and wheat tissues using the pathogen-specific primers COSA_F/R derived from the melanin biosynthesis pathway Brn1 locus. Specificity was tested against more than 30 fungal organisms associated with barley and wheat. Limit of detection was determined to be 0.001 ng for pure fungal DNA. The species-specific primers were capable of detecting the pathogen in planta, specifically from infected barley leaves and wheat roots and stem bases.
doi_str_mv 10.1016/j.cropro.2010.07.013
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subjects barley
biochemical pathways
Biosynthesis
Bipolaris sorokiniana
Cereal diseases
Cochliobolus sativus
diagnostic techniques
disease detection
disease diagnosis
Hordeum vulgare
host plants
leaves
loci
microbial genetics
Molecular diagnostics
plant pathogenic fungi
plant tissues
polymerase chain reaction
roots
Spot blotch
stems
Triticum aestivum
wheat
title Species-specific detection of Bipolaris sorokiniana from wheat and barley tissues
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