Subcellular distribution of thallium: morphological and quantitative study in rat myocardium
The purpose of this study is to determine the subcellular distribution of thallium (SDTl) by electron microscopy and a newly designed fixation method that makes insoluble grains of Tl visible. To obtain the high dose necessary for electron microscopic visualization, we employed TlCl instead of 201Tl...
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| Veröffentlicht in: | Annals of nuclear medicine 1997-12, Vol.11 (4), p.291-297 |
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| creator | Fukumoto, M Yoshida, D Yoshida, S |
| description | The purpose of this study is to determine the subcellular distribution of thallium (SDTl) by electron microscopy and a newly designed fixation method that makes insoluble grains of Tl visible.
To obtain the high dose necessary for electron microscopic visualization, we employed TlCl instead of 201TlCl. EM was performed in fixed rat myocardium resected at 20 min (early phase) and 3 hr (delay phase) after intravenous injection of TlCl. To fix Tl in the cell, we used orthovanadate in our fixative. Atomic absorption spectroscopy (AAS) of Tl and quantification of subcellular distribution of 201Tl (SD201Tl) were studied to prove the propriety of our fixation.
AAS detected Tl in the Tl-loaded specimen but not in the control, indicating that Tl was the origin of the grains observed in the former. In the early phase, numerous grains were observed in mitochondria, sarcoplasmic reticulum (SR), myofibrils, and nuclei, but no such grains were visible in controls. In the delay phase, grains were retained in mitochondria, SR and nuclei, but not in myofibrils. Electron microscopic SDTl(%) correlated with SD201Tl(%) calculated from isolated fractions.
In both the early and delay phases, mitochondria are the major site of Tl and 201Tl uptake. |
| doi_str_mv | 10.1007/bf03165296 |
| format | Article |
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To obtain the high dose necessary for electron microscopic visualization, we employed TlCl instead of 201TlCl. EM was performed in fixed rat myocardium resected at 20 min (early phase) and 3 hr (delay phase) after intravenous injection of TlCl. To fix Tl in the cell, we used orthovanadate in our fixative. Atomic absorption spectroscopy (AAS) of Tl and quantification of subcellular distribution of 201Tl (SD201Tl) were studied to prove the propriety of our fixation.
AAS detected Tl in the Tl-loaded specimen but not in the control, indicating that Tl was the origin of the grains observed in the former. In the early phase, numerous grains were observed in mitochondria, sarcoplasmic reticulum (SR), myofibrils, and nuclei, but no such grains were visible in controls. In the delay phase, grains were retained in mitochondria, SR and nuclei, but not in myofibrils. Electron microscopic SDTl(%) correlated with SD201Tl(%) calculated from isolated fractions.
In both the early and delay phases, mitochondria are the major site of Tl and 201Tl uptake.</description><identifier>ISSN: 0914-7187</identifier><identifier>EISSN: 1864-6433</identifier><identifier>DOI: 10.1007/bf03165296</identifier><identifier>PMID: 9460520</identifier><language>eng</language><publisher>Japan: Springer Nature B.V</publisher><subject>Animals ; Biological Transport ; Cell Nucleus - metabolism ; Female ; Microscopy, Electron ; Mitochondria, Heart - metabolism ; Myocardium - metabolism ; Myocardium - ultrastructure ; Myofibrils - metabolism ; Rats ; Rats, Sprague-Dawley ; Sarcoplasmic Reticulum - metabolism ; Spectrophotometry, Atomic - methods ; Thallium Radioisotopes - pharmacokinetics ; Tissue Distribution</subject><ispartof>Annals of nuclear medicine, 1997-12, Vol.11 (4), p.291-297</ispartof><rights>Springer-Verlag 1997</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c382t-6d6d927c3e2f8ae69969039fba591f80818ed6a5cfe52180fecc4d33df819ae03</citedby><cites>FETCH-LOGICAL-c382t-6d6d927c3e2f8ae69969039fba591f80818ed6a5cfe52180fecc4d33df819ae03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9460520$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fukumoto, M</creatorcontrib><creatorcontrib>Yoshida, D</creatorcontrib><creatorcontrib>Yoshida, S</creatorcontrib><title>Subcellular distribution of thallium: morphological and quantitative study in rat myocardium</title><title>Annals of nuclear medicine</title><addtitle>Ann Nucl Med</addtitle><description>The purpose of this study is to determine the subcellular distribution of thallium (SDTl) by electron microscopy and a newly designed fixation method that makes insoluble grains of Tl visible.
To obtain the high dose necessary for electron microscopic visualization, we employed TlCl instead of 201TlCl. EM was performed in fixed rat myocardium resected at 20 min (early phase) and 3 hr (delay phase) after intravenous injection of TlCl. To fix Tl in the cell, we used orthovanadate in our fixative. Atomic absorption spectroscopy (AAS) of Tl and quantification of subcellular distribution of 201Tl (SD201Tl) were studied to prove the propriety of our fixation.
AAS detected Tl in the Tl-loaded specimen but not in the control, indicating that Tl was the origin of the grains observed in the former. In the early phase, numerous grains were observed in mitochondria, sarcoplasmic reticulum (SR), myofibrils, and nuclei, but no such grains were visible in controls. In the delay phase, grains were retained in mitochondria, SR and nuclei, but not in myofibrils. Electron microscopic SDTl(%) correlated with SD201Tl(%) calculated from isolated fractions.
In both the early and delay phases, mitochondria are the major site of Tl and 201Tl uptake.</description><subject>Animals</subject><subject>Biological Transport</subject><subject>Cell Nucleus - metabolism</subject><subject>Female</subject><subject>Microscopy, Electron</subject><subject>Mitochondria, Heart - metabolism</subject><subject>Myocardium - metabolism</subject><subject>Myocardium - ultrastructure</subject><subject>Myofibrils - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Sarcoplasmic Reticulum - metabolism</subject><subject>Spectrophotometry, Atomic - methods</subject><subject>Thallium Radioisotopes - pharmacokinetics</subject><subject>Tissue Distribution</subject><issn>0914-7187</issn><issn>1864-6433</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqF0c1LHDEYBvAgle26evFeCD1UKIzmY5JJvNWlVmHBg3oThkw-amRmsuZD2P_eWdx66EFP7-X3PvDwAHCM0SlGqDnrHKKYMyL5HphjweuK15R-AXMkcV01WDRfwUFKTwgRwQSZgZmsOWIEzcHDbem07fvSqwiNTzn6rmQfRhgczI-q730ZzuEQ4vox9OGv16qHajTwuagx-6yyf7Ew5WI20I8wqgyHTdAqmunvEOw71Sd7tLsLcH_5-255Va1u_lwvf60qTQXJFTfcSNJoaokTynIpuURUuk4xiZ1AAgtruGLaWUawQM5qXRtKjRNYKovoApy85a5jeC425XbwadtKjTaU1EpWM9FMfSf540PZTLRhXH4KCcakoXgLv_8Hn0KJ41R3MgzVvMZkQj_fkI4hpWhdu45-UHHTYtRuJ2wvLv9NOOFvu8TSDda8091m9BUpcJax</recordid><startdate>19971201</startdate><enddate>19971201</enddate><creator>Fukumoto, M</creator><creator>Yoshida, D</creator><creator>Yoshida, S</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7RV</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19971201</creationdate><title>Subcellular distribution of thallium: morphological and quantitative study in rat myocardium</title><author>Fukumoto, M ; Yoshida, D ; Yoshida, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-6d6d927c3e2f8ae69969039fba591f80818ed6a5cfe52180fecc4d33df819ae03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Biological Transport</topic><topic>Cell Nucleus - metabolism</topic><topic>Female</topic><topic>Microscopy, Electron</topic><topic>Mitochondria, Heart - metabolism</topic><topic>Myocardium - metabolism</topic><topic>Myocardium - ultrastructure</topic><topic>Myofibrils - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Sarcoplasmic Reticulum - metabolism</topic><topic>Spectrophotometry, Atomic - methods</topic><topic>Thallium Radioisotopes - pharmacokinetics</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fukumoto, M</creatorcontrib><creatorcontrib>Yoshida, D</creatorcontrib><creatorcontrib>Yoshida, S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of nuclear medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fukumoto, M</au><au>Yoshida, D</au><au>Yoshida, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Subcellular distribution of thallium: morphological and quantitative study in rat myocardium</atitle><jtitle>Annals of nuclear medicine</jtitle><addtitle>Ann Nucl Med</addtitle><date>1997-12-01</date><risdate>1997</risdate><volume>11</volume><issue>4</issue><spage>291</spage><epage>297</epage><pages>291-297</pages><issn>0914-7187</issn><eissn>1864-6433</eissn><abstract>The purpose of this study is to determine the subcellular distribution of thallium (SDTl) by electron microscopy and a newly designed fixation method that makes insoluble grains of Tl visible.
To obtain the high dose necessary for electron microscopic visualization, we employed TlCl instead of 201TlCl. EM was performed in fixed rat myocardium resected at 20 min (early phase) and 3 hr (delay phase) after intravenous injection of TlCl. To fix Tl in the cell, we used orthovanadate in our fixative. Atomic absorption spectroscopy (AAS) of Tl and quantification of subcellular distribution of 201Tl (SD201Tl) were studied to prove the propriety of our fixation.
AAS detected Tl in the Tl-loaded specimen but not in the control, indicating that Tl was the origin of the grains observed in the former. In the early phase, numerous grains were observed in mitochondria, sarcoplasmic reticulum (SR), myofibrils, and nuclei, but no such grains were visible in controls. In the delay phase, grains were retained in mitochondria, SR and nuclei, but not in myofibrils. Electron microscopic SDTl(%) correlated with SD201Tl(%) calculated from isolated fractions.
In both the early and delay phases, mitochondria are the major site of Tl and 201Tl uptake.</abstract><cop>Japan</cop><pub>Springer Nature B.V</pub><pmid>9460520</pmid><doi>10.1007/bf03165296</doi><tpages>7</tpages></addata></record> |
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| ispartof | Annals of nuclear medicine, 1997-12, Vol.11 (4), p.291-297 |
| issn | 0914-7187 1864-6433 |
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| source | MEDLINE; Alma/SFX Local Collection; SpringerLink Journals - AutoHoldings |
| subjects | Animals Biological Transport Cell Nucleus - metabolism Female Microscopy, Electron Mitochondria, Heart - metabolism Myocardium - metabolism Myocardium - ultrastructure Myofibrils - metabolism Rats Rats, Sprague-Dawley Sarcoplasmic Reticulum - metabolism Spectrophotometry, Atomic - methods Thallium Radioisotopes - pharmacokinetics Tissue Distribution |
| title | Subcellular distribution of thallium: morphological and quantitative study in rat myocardium |
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