Large-scale transfection of mammalian cells for the fast production of recombinant protein
Recombinant proteins (r-proteins) are increasingly important in fundamental research and for clinical applications. As many of these r-proteins are of human or animal origin, cultivated mammalian cells are the host of choice to ensure their functional folding and proper posttranslational modificatio...
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| Veröffentlicht in: | Molecular biotechnology 2006-10, Vol.34 (2), p.225-238 |
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| creator | Pham, Phuong Lan Kamen, Amine Durocher, Yves |
| description | Recombinant proteins (r-proteins) are increasingly important in fundamental research and for clinical applications. As many of these r-proteins are of human or animal origin, cultivated mammalian cells are the host of choice to ensure their functional folding and proper posttranslational modifications. Large-scale transfection of human embryonic kidney 293 or Chinese hamster ovary cells is now an established technology that can be used in the production of hundreds of milligram to gram quantities of a r-protein in less than 1 mo from cloning of its cDNA. This chapter aims to provide an overview of large-scale transfection technology with a particular emphasis on calcium phosphate and polyethylenimine-mediated gene transfer. |
| doi_str_mv | 10.1385/mb:34:2:225 |
| format | Article |
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| ispartof | Molecular biotechnology, 2006-10, Vol.34 (2), p.225-238 |
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| subjects | Animals Biotechnology - methods Calcium phosphates Calcium Phosphates - pharmacology Cell Line Cells Cloning Cricetinae Culture Media Genetic Vectors - genetics Humans Kidneys Mammals Polyethyleneimine - pharmacology Proteins Recombinant Proteins - biosynthesis Studies Transfection - methods |
| title | Large-scale transfection of mammalian cells for the fast production of recombinant protein |
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