Purification and characterization of a microbial dehydrogenase. A vanillin:NAD(P) super(+) oxidoreductase

Pseudomonas fluorescens (strain BTP9) was found to have at least two NAD(P)-dependent vanillin dehydrogenases: one is induced by vanillin, and the other is constitutive. The constitutive enzyme was purified by ammonium sulfate fractionation, gel-filtration, and Q-Sepharose chromatography. The subuni...

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Veröffentlicht in:Applied biochemistry and biotechnology 2002-03, Vol.98-100 (1-9), p.415-428
Hauptverfasser: Bare, Ghislain, Swiatkowski, Thierry, Moukil, Ahmed, Gerday, Charles, Thonart, Philippe
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Sprache:eng
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