High temperature cDNA synthesis by AMV reverse transcriptase improves the specificity of PCR
The enzyme avian myeloblastosis virus reverse transcriptase (AMV-RT) is routinely used for cDNA synthesis, which is generally carried out at temperatures between 37 degrees C and 42 degrees C. We show that this enzyme can support cDNA synthesis, at temperatures as high as 70 degrees C. We have utili...
Gespeichert in:
| Veröffentlicht in: | Molecular biotechnology 1999-10, Vol.12 (3), p.237-240 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 240 |
|---|---|
| container_issue | 3 |
| container_start_page | 237 |
| container_title | Molecular biotechnology |
| container_volume | 12 |
| creator | FUCHS, B KUNBO ZHANG ROCK, M. G BOLANDER, M. E SARKAR, G |
| description | The enzyme avian myeloblastosis virus reverse transcriptase (AMV-RT) is routinely used for cDNA synthesis, which is generally carried out at temperatures between 37 degrees C and 42 degrees C. We show that this enzyme can support cDNA synthesis, at temperatures as high as 70 degrees C. We have utilized this property of the AMV-RT to improve the specificity of polymerase chain reaction (PCR). Furthermore, this apparently thermophilic property of the enzyme, which is an important constituent of a mesophilic organism, raises intriguing questions regarding evolution of the enzyme structure. |
| doi_str_mv | 10.1385/MB:12:3:237 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_954581465</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>69400753</sourcerecordid><originalsourceid>FETCH-LOGICAL-c404t-b433aeb50f595c0b22c2e5664f3c31ecfdfaa6a3227c4121e526603446b9ff663</originalsourceid><addsrcrecordid>eNqF0c9rFDEUB_Agiq3Vk3cJUuyhjCZ5-TGzt-3aWqGrIupJCJn0xabs7IzJTGH_e7PsgqUHPeUFPvnywpeQl5y95VCrd8uzGRczmAkwj8ghV6qpGDD1uMzMQKVZrQ7Is5xvGRNcSXhKDjjTwHXNDsnPy_jrho7YDZjcOCWk_v2nOc2b9XiDOWbabuh8-YMmvMOUkY7JrbNPcRhducVuSP0dZlowzQP6GKKP44b2gX5ZfH1OngS3yvhifx6R7xfn3xaX1dXnDx8X86vKSybHqpUADlvFgmqUZ60QXqDSWgbwwNGH6-CcdiCE8ZILjkpozUBK3TYhaA1H5GSXW7b5PWEebRezx9XKrbGfsm2UVDWXWhX55p9SN5Ixo-C_kBspec1Nga8fwNt-SuvyXStAG1ObZpt2ukM-9TknDHZIsXNpYzmz2xLt8sxyYaG82Ua-2kdObYfX9-yutQKO98Bl71ahdOJj_uuEkAYa-AOfbKI7</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>236778793</pqid></control><display><type>article</type><title>High temperature cDNA synthesis by AMV reverse transcriptase improves the specificity of PCR</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>FUCHS, B ; KUNBO ZHANG ; ROCK, M. G ; BOLANDER, M. E ; SARKAR, G</creator><creatorcontrib>FUCHS, B ; KUNBO ZHANG ; ROCK, M. G ; BOLANDER, M. E ; SARKAR, G</creatorcontrib><description>The enzyme avian myeloblastosis virus reverse transcriptase (AMV-RT) is routinely used for cDNA synthesis, which is generally carried out at temperatures between 37 degrees C and 42 degrees C. We show that this enzyme can support cDNA synthesis, at temperatures as high as 70 degrees C. We have utilized this property of the AMV-RT to improve the specificity of polymerase chain reaction (PCR). Furthermore, this apparently thermophilic property of the enzyme, which is an important constituent of a mesophilic organism, raises intriguing questions regarding evolution of the enzyme structure.</description><identifier>ISSN: 1073-6085</identifier><identifier>EISSN: 1559-0305</identifier><identifier>EISSN: 1073-6085</identifier><identifier>DOI: 10.1385/MB:12:3:237</identifier><identifier>PMID: 10631680</identifier><identifier>CODEN: MLBOEO</identifier><language>eng</language><publisher>Totowa, NJ: Humana Press</publisher><subject>Avian myeloblastosis ; Avian myeloblastosis virus ; Avian Myeloblastosis Virus - enzymology ; Biological and medical sciences ; Deoxyribonucleic acid ; Diverse techniques ; DNA ; DNA, Complementary - chemical synthesis ; Enzymes ; Evolution ; Fundamental and applied biological sciences. Psychology ; High temperature ; Humans ; Molecular and cellular biology ; Polymerase chain reaction ; Polymerase Chain Reaction - methods ; RNA-directed DNA polymerase ; RNA-Directed DNA Polymerase - chemistry ; RNA-Directed DNA Polymerase - metabolism ; Sensitivity and Specificity ; Studies ; Temperature ; Temperature effects ; Templates, Genetic ; Viruses</subject><ispartof>Molecular biotechnology, 1999-10, Vol.12 (3), p.237-240</ispartof><rights>2000 INIST-CNRS</rights><rights>Humana Press Inc. 1999</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c404t-b433aeb50f595c0b22c2e5664f3c31ecfdfaa6a3227c4121e526603446b9ff663</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1224739$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10631680$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>FUCHS, B</creatorcontrib><creatorcontrib>KUNBO ZHANG</creatorcontrib><creatorcontrib>ROCK, M. G</creatorcontrib><creatorcontrib>BOLANDER, M. E</creatorcontrib><creatorcontrib>SARKAR, G</creatorcontrib><title>High temperature cDNA synthesis by AMV reverse transcriptase improves the specificity of PCR</title><title>Molecular biotechnology</title><addtitle>Mol Biotechnol</addtitle><description>The enzyme avian myeloblastosis virus reverse transcriptase (AMV-RT) is routinely used for cDNA synthesis, which is generally carried out at temperatures between 37 degrees C and 42 degrees C. We show that this enzyme can support cDNA synthesis, at temperatures as high as 70 degrees C. We have utilized this property of the AMV-RT to improve the specificity of polymerase chain reaction (PCR). Furthermore, this apparently thermophilic property of the enzyme, which is an important constituent of a mesophilic organism, raises intriguing questions regarding evolution of the enzyme structure.</description><subject>Avian myeloblastosis</subject><subject>Avian myeloblastosis virus</subject><subject>Avian Myeloblastosis Virus - enzymology</subject><subject>Biological and medical sciences</subject><subject>Deoxyribonucleic acid</subject><subject>Diverse techniques</subject><subject>DNA</subject><subject>DNA, Complementary - chemical synthesis</subject><subject>Enzymes</subject><subject>Evolution</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>High temperature</subject><subject>Humans</subject><subject>Molecular and cellular biology</subject><subject>Polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>RNA-directed DNA polymerase</subject><subject>RNA-Directed DNA Polymerase - chemistry</subject><subject>RNA-Directed DNA Polymerase - metabolism</subject><subject>Sensitivity and Specificity</subject><subject>Studies</subject><subject>Temperature</subject><subject>Temperature effects</subject><subject>Templates, Genetic</subject><subject>Viruses</subject><issn>1073-6085</issn><issn>1559-0305</issn><issn>1073-6085</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqF0c9rFDEUB_Agiq3Vk3cJUuyhjCZ5-TGzt-3aWqGrIupJCJn0xabs7IzJTGH_e7PsgqUHPeUFPvnywpeQl5y95VCrd8uzGRczmAkwj8ghV6qpGDD1uMzMQKVZrQ7Is5xvGRNcSXhKDjjTwHXNDsnPy_jrho7YDZjcOCWk_v2nOc2b9XiDOWbabuh8-YMmvMOUkY7JrbNPcRhducVuSP0dZlowzQP6GKKP44b2gX5ZfH1OngS3yvhifx6R7xfn3xaX1dXnDx8X86vKSybHqpUADlvFgmqUZ60QXqDSWgbwwNGH6-CcdiCE8ZILjkpozUBK3TYhaA1H5GSXW7b5PWEebRezx9XKrbGfsm2UVDWXWhX55p9SN5Ixo-C_kBspec1Nga8fwNt-SuvyXStAG1ObZpt2ukM-9TknDHZIsXNpYzmz2xLt8sxyYaG82Ua-2kdObYfX9-yutQKO98Bl71ahdOJj_uuEkAYa-AOfbKI7</recordid><startdate>19991001</startdate><enddate>19991001</enddate><creator>FUCHS, B</creator><creator>KUNBO ZHANG</creator><creator>ROCK, M. G</creator><creator>BOLANDER, M. E</creator><creator>SARKAR, G</creator><general>Humana Press</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>7X8</scope><scope>7TM</scope></search><sort><creationdate>19991001</creationdate><title>High temperature cDNA synthesis by AMV reverse transcriptase improves the specificity of PCR</title><author>FUCHS, B ; KUNBO ZHANG ; ROCK, M. G ; BOLANDER, M. E ; SARKAR, G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-b433aeb50f595c0b22c2e5664f3c31ecfdfaa6a3227c4121e526603446b9ff663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Avian myeloblastosis</topic><topic>Avian myeloblastosis virus</topic><topic>Avian Myeloblastosis Virus - enzymology</topic><topic>Biological and medical sciences</topic><topic>Deoxyribonucleic acid</topic><topic>Diverse techniques</topic><topic>DNA</topic><topic>DNA, Complementary - chemical synthesis</topic><topic>Enzymes</topic><topic>Evolution</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>High temperature</topic><topic>Humans</topic><topic>Molecular and cellular biology</topic><topic>Polymerase chain reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>RNA-directed DNA polymerase</topic><topic>RNA-Directed DNA Polymerase - chemistry</topic><topic>RNA-Directed DNA Polymerase - metabolism</topic><topic>Sensitivity and Specificity</topic><topic>Studies</topic><topic>Temperature</topic><topic>Temperature effects</topic><topic>Templates, Genetic</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>FUCHS, B</creatorcontrib><creatorcontrib>KUNBO ZHANG</creatorcontrib><creatorcontrib>ROCK, M. G</creatorcontrib><creatorcontrib>BOLANDER, M. E</creatorcontrib><creatorcontrib>SARKAR, G</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Molecular biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>FUCHS, B</au><au>KUNBO ZHANG</au><au>ROCK, M. G</au><au>BOLANDER, M. E</au><au>SARKAR, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High temperature cDNA synthesis by AMV reverse transcriptase improves the specificity of PCR</atitle><jtitle>Molecular biotechnology</jtitle><addtitle>Mol Biotechnol</addtitle><date>1999-10-01</date><risdate>1999</risdate><volume>12</volume><issue>3</issue><spage>237</spage><epage>240</epage><pages>237-240</pages><issn>1073-6085</issn><eissn>1559-0305</eissn><eissn>1073-6085</eissn><coden>MLBOEO</coden><abstract>The enzyme avian myeloblastosis virus reverse transcriptase (AMV-RT) is routinely used for cDNA synthesis, which is generally carried out at temperatures between 37 degrees C and 42 degrees C. We show that this enzyme can support cDNA synthesis, at temperatures as high as 70 degrees C. We have utilized this property of the AMV-RT to improve the specificity of polymerase chain reaction (PCR). Furthermore, this apparently thermophilic property of the enzyme, which is an important constituent of a mesophilic organism, raises intriguing questions regarding evolution of the enzyme structure.</abstract><cop>Totowa, NJ</cop><pub>Humana Press</pub><pmid>10631680</pmid><doi>10.1385/MB:12:3:237</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1073-6085 |
| ispartof | Molecular biotechnology, 1999-10, Vol.12 (3), p.237-240 |
| issn | 1073-6085 1559-0305 1073-6085 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_954581465 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Avian myeloblastosis Avian myeloblastosis virus Avian Myeloblastosis Virus - enzymology Biological and medical sciences Deoxyribonucleic acid Diverse techniques DNA DNA, Complementary - chemical synthesis Enzymes Evolution Fundamental and applied biological sciences. Psychology High temperature Humans Molecular and cellular biology Polymerase chain reaction Polymerase Chain Reaction - methods RNA-directed DNA polymerase RNA-Directed DNA Polymerase - chemistry RNA-Directed DNA Polymerase - metabolism Sensitivity and Specificity Studies Temperature Temperature effects Templates, Genetic Viruses |
| title | High temperature cDNA synthesis by AMV reverse transcriptase improves the specificity of PCR |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T03%3A13%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=High%20temperature%20cDNA%20synthesis%20by%20AMV%20reverse%20transcriptase%20improves%20the%20specificity%20of%20PCR&rft.jtitle=Molecular%20biotechnology&rft.au=FUCHS,%20B&rft.date=1999-10-01&rft.volume=12&rft.issue=3&rft.spage=237&rft.epage=240&rft.pages=237-240&rft.issn=1073-6085&rft.eissn=1559-0305&rft.coden=MLBOEO&rft_id=info:doi/10.1385/MB:12:3:237&rft_dat=%3Cproquest_cross%3E69400753%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=236778793&rft_id=info:pmid/10631680&rfr_iscdi=true |