Effects of Different Oocyte Activation Procedures on Development and Gene Expression of Porcine Pre-Implantation Embryos
Contents Among the factors that affect the efficiency of somatic cell nuclear transfer (SCNT) in pigs, the activation protocol is the most variable among the current SCNT procedures. The aim of this study is focused on defining an efficient activation treatment of porcine oocytes. In Experiment 1, w...
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| Veröffentlicht in: | Reproduction in domestic animals 2010-10, Vol.45 (5), p.e12-e20 |
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| container_title | Reproduction in domestic animals |
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| creator | Cervera, RP Silvestre, MA Martí, N García-Mengual, E Moreno, R Stojkovic, M |
| description | Contents
Among the factors that affect the efficiency of somatic cell nuclear transfer (SCNT) in pigs, the activation protocol is the most variable among the current SCNT procedures. The aim of this study is focused on defining an efficient activation treatment of porcine oocytes. In Experiment 1, we studied the effects of nine different oocyte activation procedures (including chemical‐ and electrical‐based treatments) on parthenogenetic embryo development. In Experiment 2, we studied the effect of the more efficient activation procedures on the gene expression profile of Oct4 and Igf2r in parthenogenetic blastocysts. In conclusion, ionomycin as a first calcium stimulus is not able to activate porcine oocytes efficiently in comparison with electric procedures. Electrical treatments with 6‐DMAP significantly increased the level of Oct4 expression, whereas the single and double pulse treatments alone maintained the same profile as the IVF group. |
| doi_str_mv | 10.1111/j.1439-0531.2009.01509.x |
| format | Article |
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Among the factors that affect the efficiency of somatic cell nuclear transfer (SCNT) in pigs, the activation protocol is the most variable among the current SCNT procedures. The aim of this study is focused on defining an efficient activation treatment of porcine oocytes. In Experiment 1, we studied the effects of nine different oocyte activation procedures (including chemical‐ and electrical‐based treatments) on parthenogenetic embryo development. In Experiment 2, we studied the effect of the more efficient activation procedures on the gene expression profile of Oct4 and Igf2r in parthenogenetic blastocysts. In conclusion, ionomycin as a first calcium stimulus is not able to activate porcine oocytes efficiently in comparison with electric procedures. Electrical treatments with 6‐DMAP significantly increased the level of Oct4 expression, whereas the single and double pulse treatments alone maintained the same profile as the IVF group.</description><identifier>ISSN: 0936-6768</identifier><identifier>EISSN: 1439-0531</identifier><identifier>DOI: 10.1111/j.1439-0531.2009.01509.x</identifier><identifier>PMID: 19663811</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animal reproduction ; Animals ; Blastocyst - drug effects ; Blastocyst - physiology ; blastocysts ; Calcium ; Electric Stimulation ; Embryonic Development - drug effects ; Embryonic Development - physiology ; Embryos ; Gene expression ; Gene Expression Profiling - veterinary ; Gene Expression Regulation, Developmental - physiology ; Insulin-like growth factor II receptors ; Ionomycin ; Nuclear Transfer Techniques - veterinary ; Oct-4 protein ; Octamer Transcription Factors - genetics ; Octamer Transcription Factors - metabolism ; Oocytes ; Oocytes - physiology ; somatic cell nuclear transfer ; Swine - embryology ; Transplants & implants</subject><ispartof>Reproduction in domestic animals, 2010-10, Vol.45 (5), p.e12-e20</ispartof><rights>2009 Blackwell Verlag GmbH</rights><rights>2009 Blackwell Verlag GmbH.</rights><rights>2010 Blackwell Verlag GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4659-60cb4191faf689711e5eab895dc2e84ffed04252b2d7cc7aeece278a027b373f3</citedby><cites>FETCH-LOGICAL-c4659-60cb4191faf689711e5eab895dc2e84ffed04252b2d7cc7aeece278a027b373f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1439-0531.2009.01509.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1439-0531.2009.01509.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19663811$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cervera, RP</creatorcontrib><creatorcontrib>Silvestre, MA</creatorcontrib><creatorcontrib>Martí, N</creatorcontrib><creatorcontrib>García-Mengual, E</creatorcontrib><creatorcontrib>Moreno, R</creatorcontrib><creatorcontrib>Stojkovic, M</creatorcontrib><title>Effects of Different Oocyte Activation Procedures on Development and Gene Expression of Porcine Pre-Implantation Embryos</title><title>Reproduction in domestic animals</title><addtitle>Reprod Domest Anim</addtitle><description>Contents
Among the factors that affect the efficiency of somatic cell nuclear transfer (SCNT) in pigs, the activation protocol is the most variable among the current SCNT procedures. The aim of this study is focused on defining an efficient activation treatment of porcine oocytes. In Experiment 1, we studied the effects of nine different oocyte activation procedures (including chemical‐ and electrical‐based treatments) on parthenogenetic embryo development. In Experiment 2, we studied the effect of the more efficient activation procedures on the gene expression profile of Oct4 and Igf2r in parthenogenetic blastocysts. In conclusion, ionomycin as a first calcium stimulus is not able to activate porcine oocytes efficiently in comparison with electric procedures. Electrical treatments with 6‐DMAP significantly increased the level of Oct4 expression, whereas the single and double pulse treatments alone maintained the same profile as the IVF group.</description><subject>Animal reproduction</subject><subject>Animals</subject><subject>Blastocyst - drug effects</subject><subject>Blastocyst - physiology</subject><subject>blastocysts</subject><subject>Calcium</subject><subject>Electric Stimulation</subject><subject>Embryonic Development - drug effects</subject><subject>Embryonic Development - physiology</subject><subject>Embryos</subject><subject>Gene expression</subject><subject>Gene Expression Profiling - veterinary</subject><subject>Gene Expression Regulation, Developmental - physiology</subject><subject>Insulin-like growth factor II receptors</subject><subject>Ionomycin</subject><subject>Nuclear Transfer Techniques - veterinary</subject><subject>Oct-4 protein</subject><subject>Octamer Transcription Factors - genetics</subject><subject>Octamer Transcription Factors - metabolism</subject><subject>Oocytes</subject><subject>Oocytes - physiology</subject><subject>somatic cell nuclear transfer</subject><subject>Swine - embryology</subject><subject>Transplants & implants</subject><issn>0936-6768</issn><issn>1439-0531</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU9v1DAQxS0EokvhK6CIC6ds7Tj-d-Cw6i5LpVW7qqAcLceZSFmSeLGTkv32OGTVSlzAB3vk-b2nGT2EEoKXJJ6rw5LkVKWYUbLMMFZLTFi8xxdo8dR4iRZYUZ5yweUFehPCAUdKCvEaXRDFOZWELNC4qSqwfUhclazrWHvo-uTO2VMPycr29aPpa9cle-8slIOHSHbJGh6hccd2Yk1XJlvoINmMx9gOEx3N9s7bOv7uPaQ37bExXT87bdrCn1x4i15Vpgnw7vxeom-fN1-vv6S7u-3N9WqX2pwzlXJsi5woUpmKSyUIAQamkIqVNgOZx4FLnGcsK7JSWCsMgIVMSIMzUVBBK3qJPs6-R-9-DhB63dbBQhMHAjcErVjOhMRE_ZOUHBPJc55F8sNf5MENvotraMGozLBiJEJyhqx3IXio9NHXrfEnTbCeUtQHPYWlp7D0lKL-k6Ieo_T92X8oWiifhefYIvBpBn7VDZz-21jfr1dTFfXprK9DD-OT3vgfmgsqmP5-u9W7B3IrHu53WtDfsoO7Mw</recordid><startdate>201010</startdate><enddate>201010</enddate><creator>Cervera, RP</creator><creator>Silvestre, MA</creator><creator>Martí, N</creator><creator>García-Mengual, E</creator><creator>Moreno, R</creator><creator>Stojkovic, M</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><scope>RC3</scope></search><sort><creationdate>201010</creationdate><title>Effects of Different Oocyte Activation Procedures on Development and Gene Expression of Porcine Pre-Implantation Embryos</title><author>Cervera, RP ; Silvestre, MA ; Martí, N ; García-Mengual, E ; Moreno, R ; Stojkovic, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4659-60cb4191faf689711e5eab895dc2e84ffed04252b2d7cc7aeece278a027b373f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animal reproduction</topic><topic>Animals</topic><topic>Blastocyst - drug effects</topic><topic>Blastocyst - physiology</topic><topic>blastocysts</topic><topic>Calcium</topic><topic>Electric Stimulation</topic><topic>Embryonic Development - drug effects</topic><topic>Embryonic Development - physiology</topic><topic>Embryos</topic><topic>Gene expression</topic><topic>Gene Expression Profiling - veterinary</topic><topic>Gene Expression Regulation, Developmental - physiology</topic><topic>Insulin-like growth factor II receptors</topic><topic>Ionomycin</topic><topic>Nuclear Transfer Techniques - veterinary</topic><topic>Oct-4 protein</topic><topic>Octamer Transcription Factors - genetics</topic><topic>Octamer Transcription Factors - metabolism</topic><topic>Oocytes</topic><topic>Oocytes - physiology</topic><topic>somatic cell nuclear transfer</topic><topic>Swine - embryology</topic><topic>Transplants & implants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cervera, RP</creatorcontrib><creatorcontrib>Silvestre, MA</creatorcontrib><creatorcontrib>Martí, N</creatorcontrib><creatorcontrib>García-Mengual, E</creatorcontrib><creatorcontrib>Moreno, R</creatorcontrib><creatorcontrib>Stojkovic, M</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Genetics Abstracts</collection><jtitle>Reproduction in domestic animals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cervera, RP</au><au>Silvestre, MA</au><au>Martí, N</au><au>García-Mengual, E</au><au>Moreno, R</au><au>Stojkovic, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Different Oocyte Activation Procedures on Development and Gene Expression of Porcine Pre-Implantation Embryos</atitle><jtitle>Reproduction in domestic animals</jtitle><addtitle>Reprod Domest Anim</addtitle><date>2010-10</date><risdate>2010</risdate><volume>45</volume><issue>5</issue><spage>e12</spage><epage>e20</epage><pages>e12-e20</pages><issn>0936-6768</issn><eissn>1439-0531</eissn><abstract>Contents
Among the factors that affect the efficiency of somatic cell nuclear transfer (SCNT) in pigs, the activation protocol is the most variable among the current SCNT procedures. The aim of this study is focused on defining an efficient activation treatment of porcine oocytes. In Experiment 1, we studied the effects of nine different oocyte activation procedures (including chemical‐ and electrical‐based treatments) on parthenogenetic embryo development. In Experiment 2, we studied the effect of the more efficient activation procedures on the gene expression profile of Oct4 and Igf2r in parthenogenetic blastocysts. In conclusion, ionomycin as a first calcium stimulus is not able to activate porcine oocytes efficiently in comparison with electric procedures. Electrical treatments with 6‐DMAP significantly increased the level of Oct4 expression, whereas the single and double pulse treatments alone maintained the same profile as the IVF group.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>19663811</pmid><doi>10.1111/j.1439-0531.2009.01509.x</doi><tpages>9</tpages></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animal reproduction Animals Blastocyst - drug effects Blastocyst - physiology blastocysts Calcium Electric Stimulation Embryonic Development - drug effects Embryonic Development - physiology Embryos Gene expression Gene Expression Profiling - veterinary Gene Expression Regulation, Developmental - physiology Insulin-like growth factor II receptors Ionomycin Nuclear Transfer Techniques - veterinary Oct-4 protein Octamer Transcription Factors - genetics Octamer Transcription Factors - metabolism Oocytes Oocytes - physiology somatic cell nuclear transfer Swine - embryology Transplants & implants |
| title | Effects of Different Oocyte Activation Procedures on Development and Gene Expression of Porcine Pre-Implantation Embryos |
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