Cloning, co-expression with an amidating enzyme, and activity of the scorpion toxin BmK ITa1 cDNA in insect cells

BmK ITa1 is an insect-specific neurotoxin from the Chinese scorpion Buthus martensi Karsch (Bmk). We succeeded in obtaining biologically active recombinant BmK ITa1 protein by simultaneous expression in insect cells of BmK ITa1 cDNA with an amidating enzyme expressed by the rat peptidylglycine alpha...

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Veröffentlicht in:	Molecular biotechnology 2003-05, Vol.24 (1), p.21-26
Hauptverfasser:	Liu, Zhen, Yang, Guanzhen, Li, Boliang, Chi, Chengwu, Wu, Xiangfu
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Baculoviridae - genetics Baculoviridae - metabolism Biological and medical sciences Biotechnology Bombyx - drug effects Bombyx mori Buthus martensii Cloning Cloning, Molecular Dose-Response Relationship, Drug Enzymes Fundamental and applied biological sciences. Psychology Gene Expression Regulation - genetics Insects Larva - drug effects Larvae Lethal Dose 50 Methods. Procedures. Technologies Mixed Function Oxygenases - biosynthesis Mixed Function Oxygenases - genetics Multienzyme Complexes - biosynthesis Multienzyme Complexes - genetics Neurotoxins Protein engineering Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Recombinant Proteins - pharmacology Scorpion Venoms - biosynthesis Scorpion Venoms - genetics Scorpion Venoms - isolation & purification Scorpion Venoms - pharmacology Toxins
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