Molecular characterization of Kluyveromyces marxianus strains isolated from Agave fourcroydes (Lem.) in Yucatan, Mexico
The molecular characterization of 14 strains of Kluyveromyces marxianus isolated from Agave fourcroydes (Lem.) in Yucatan, Mexico, was performed by AP-PCR analysis, PCR-RFLP of 5.8S-ITS, and complete NTS regions. A sequence analysis of the D1/D2 domain of the 26S rDNA was also carried out in six sel...
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| Veröffentlicht in: | Molecular biotechnology 2007-11, Vol.37 (3), p.181-186 |
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| creator | PEREZ-BRITO, Daisy TAPIA-TUSSELL, Raul QUIJANO-RAMAYO, Andrés LARQUE-SAAVEDRA, Alfonso LAPPE, Patricia |
| description | The molecular characterization of 14 strains of Kluyveromyces marxianus isolated from Agave fourcroydes (Lem.) in Yucatan, Mexico, was performed by AP-PCR analysis, PCR-RFLP of 5.8S-ITS, and complete NTS regions. A sequence analysis of the D1/D2 domain of the 26S rDNA was also carried out in six selected strains. The AP-PCR approach had the highest discrimination power for the molecular characterization of new henequen K. marxianus strains. PCR-RFLP of 5.8S-ITS regions did not reveal polymorphisms in this group of strains. The restriction enzyme digestion analysis of NTS region enables the separation among strains which coincides with ascospore shape groups. The molecular tools used in this article may be useful to confirm a preliminary screen of yeasts isolated from henequen without the use of growth characteristics or morpho-physiological tests. |
| doi_str_mv | 10.1007/s12033-007-0036-y |
| format | Article |
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A sequence analysis of the D1/D2 domain of the 26S rDNA was also carried out in six selected strains. The AP-PCR approach had the highest discrimination power for the molecular characterization of new henequen K. marxianus strains. PCR-RFLP of 5.8S-ITS regions did not reveal polymorphisms in this group of strains. The restriction enzyme digestion analysis of NTS region enables the separation among strains which coincides with ascospore shape groups. The molecular tools used in this article may be useful to confirm a preliminary screen of yeasts isolated from henequen without the use of growth characteristics or morpho-physiological tests.</description><identifier>ISSN: 1073-6085</identifier><identifier>EISSN: 1559-0305</identifier><identifier>DOI: 10.1007/s12033-007-0036-y</identifier><identifier>PMID: 17952662</identifier><identifier>CODEN: MLBOEO</identifier><language>eng</language><publisher>Totowa, NJ: Humana Press</publisher><subject>Agave - microbiology ; Agave fourcroydes ; Biological and medical sciences ; Biotechnology ; DNA, Ribosomal - analysis ; Fundamental and applied biological sciences. Psychology ; Kluyveromyces - genetics ; Kluyveromyces - isolation & purification ; Kluyveromyces marxianus ; Mexico ; Molecular biology ; Polymerase Chain Reaction - methods ; Polymorphism, Restriction Fragment Length ; Sequence Analysis, DNA ; Studies ; Yeast ; Yeasts</subject><ispartof>Molecular biotechnology, 2007-11, Vol.37 (3), p.181-186</ispartof><rights>2008 INIST-CNRS</rights><rights>Humana Press 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-b4d819b22a884dc4ecb1220b3a5c95ba520c12b0f26486c292b7388604ca18a13</citedby><cites>FETCH-LOGICAL-c388t-b4d819b22a884dc4ecb1220b3a5c95ba520c12b0f26486c292b7388604ca18a13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19281386$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17952662$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>PEREZ-BRITO, Daisy</creatorcontrib><creatorcontrib>TAPIA-TUSSELL, Raul</creatorcontrib><creatorcontrib>QUIJANO-RAMAYO, Andrés</creatorcontrib><creatorcontrib>LARQUE-SAAVEDRA, Alfonso</creatorcontrib><creatorcontrib>LAPPE, Patricia</creatorcontrib><title>Molecular characterization of Kluyveromyces marxianus strains isolated from Agave fourcroydes (Lem.) in Yucatan, Mexico</title><title>Molecular biotechnology</title><addtitle>Mol Biotechnol</addtitle><description>The molecular characterization of 14 strains of Kluyveromyces marxianus isolated from Agave fourcroydes (Lem.) in Yucatan, Mexico, was performed by AP-PCR analysis, PCR-RFLP of 5.8S-ITS, and complete NTS regions. A sequence analysis of the D1/D2 domain of the 26S rDNA was also carried out in six selected strains. The AP-PCR approach had the highest discrimination power for the molecular characterization of new henequen K. marxianus strains. PCR-RFLP of 5.8S-ITS regions did not reveal polymorphisms in this group of strains. The restriction enzyme digestion analysis of NTS region enables the separation among strains which coincides with ascospore shape groups. The molecular tools used in this article may be useful to confirm a preliminary screen of yeasts isolated from henequen without the use of growth characteristics or morpho-physiological tests.</description><subject>Agave - microbiology</subject><subject>Agave fourcroydes</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>DNA, Ribosomal - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Kluyveromyces - genetics</subject><subject>Kluyveromyces - isolation & purification</subject><subject>Kluyveromyces marxianus</subject><subject>Mexico</subject><subject>Molecular biology</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Sequence Analysis, DNA</subject><subject>Studies</subject><subject>Yeast</subject><subject>Yeasts</subject><issn>1073-6085</issn><issn>1559-0305</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kc1O3TAQhS3UCijtA3RTWZX6g9TA2I4dZ4lQWxAXsaELVtbEcVqjJAY7oYSnx1f3SkhddDGas_jOkWYOIe8ZHDGA6jgxDkIUWeYRqlh2yD6Tsi5AgHyVNVSiUKDlHnmT0i0AZ7IUu2SPVbXkSvF98vcy9M7OPUZq_2BEO7non3DyYaShoxf9vDy4GIbFukQHjI8exznRNEX0Y6I-hR4n19IuM_TkNz442oU52hiWNju-rtxwdEj9SG9mixOO3-ile_Q2vCWvO-yTe7fdB-TXj-_Xp2fF6urn-enJqrBC66loylazuuEctS5bWzrbMM6hEShtLRuUHCzjDXRclVpZXvOmykYFpUWmkYkD8mWTexfD_ezSZAafrOt7HF2Yk6llKatKSp3Jz_8llS4ZFxVk8OM_4G2-eMxXGC5UpWuAdRrbQPkTKUXXmbvo8_8Ww8CsyzOb8sxarsszS_Z82AbPzeDaF8e2rQx82gKYLPZdxNH69MLVXDOhlXgGPs2iVg</recordid><startdate>20071101</startdate><enddate>20071101</enddate><creator>PEREZ-BRITO, Daisy</creator><creator>TAPIA-TUSSELL, Raul</creator><creator>QUIJANO-RAMAYO, Andrés</creator><creator>LARQUE-SAAVEDRA, Alfonso</creator><creator>LAPPE, Patricia</creator><general>Humana Press</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20071101</creationdate><title>Molecular characterization of Kluyveromyces marxianus strains isolated from Agave fourcroydes (Lem.) in Yucatan, Mexico</title><author>PEREZ-BRITO, Daisy ; TAPIA-TUSSELL, Raul ; QUIJANO-RAMAYO, Andrés ; LARQUE-SAAVEDRA, Alfonso ; LAPPE, Patricia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-b4d819b22a884dc4ecb1220b3a5c95ba520c12b0f26486c292b7388604ca18a13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Agave - microbiology</topic><topic>Agave fourcroydes</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>DNA, Ribosomal - analysis</topic><topic>Fundamental and applied biological sciences. 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Academic</collection><jtitle>Molecular biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>PEREZ-BRITO, Daisy</au><au>TAPIA-TUSSELL, Raul</au><au>QUIJANO-RAMAYO, Andrés</au><au>LARQUE-SAAVEDRA, Alfonso</au><au>LAPPE, Patricia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular characterization of Kluyveromyces marxianus strains isolated from Agave fourcroydes (Lem.) in Yucatan, Mexico</atitle><jtitle>Molecular biotechnology</jtitle><addtitle>Mol Biotechnol</addtitle><date>2007-11-01</date><risdate>2007</risdate><volume>37</volume><issue>3</issue><spage>181</spage><epage>186</epage><pages>181-186</pages><issn>1073-6085</issn><eissn>1559-0305</eissn><coden>MLBOEO</coden><abstract>The molecular characterization of 14 strains of Kluyveromyces marxianus isolated from Agave fourcroydes (Lem.) in Yucatan, Mexico, was performed by AP-PCR analysis, PCR-RFLP of 5.8S-ITS, and complete NTS regions. A sequence analysis of the D1/D2 domain of the 26S rDNA was also carried out in six selected strains. The AP-PCR approach had the highest discrimination power for the molecular characterization of new henequen K. marxianus strains. PCR-RFLP of 5.8S-ITS regions did not reveal polymorphisms in this group of strains. The restriction enzyme digestion analysis of NTS region enables the separation among strains which coincides with ascospore shape groups. The molecular tools used in this article may be useful to confirm a preliminary screen of yeasts isolated from henequen without the use of growth characteristics or morpho-physiological tests.</abstract><cop>Totowa, NJ</cop><pub>Humana Press</pub><pmid>17952662</pmid><doi>10.1007/s12033-007-0036-y</doi><tpages>6</tpages></addata></record> |
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| ispartof | Molecular biotechnology, 2007-11, Vol.37 (3), p.181-186 |
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| subjects | Agave - microbiology Agave fourcroydes Biological and medical sciences Biotechnology DNA, Ribosomal - analysis Fundamental and applied biological sciences. Psychology Kluyveromyces - genetics Kluyveromyces - isolation & purification Kluyveromyces marxianus Mexico Molecular biology Polymerase Chain Reaction - methods Polymorphism, Restriction Fragment Length Sequence Analysis, DNA Studies Yeast Yeasts |
| title | Molecular characterization of Kluyveromyces marxianus strains isolated from Agave fourcroydes (Lem.) in Yucatan, Mexico |
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