Expression of Apoptosis Regulatory Genes and Incidence of Apoptosis in Different Morphological Quality Groups of In Vitro-produced Bovine Pre-implantation Embryos
Apoptosis occurs during early development in both in vivo- and in vitro-produced embryos, and is considered as one of the causes of embryonic loss. The objectives of this study were, therefore, investigating stage-specific expression profiles of apoptosis regulatory genes in three quality groups of...
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| Veröffentlicht in: | Reproduction in domestic animals 2010-10, Vol.45 (5), p.915-921 |
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| creator | Melka, MG Rings, F Hölker, M Tholen, E Havlicek, V Besenfelder, U Schellander, K Tesfaye, D |
| description | Apoptosis occurs during early development in both in vivo- and in vitro-produced embryos, and is considered as one of the causes of embryonic loss. The objectives of this study were, therefore, investigating stage-specific expression profiles of apoptosis regulatory genes in three quality groups of in vitro-produced bovine pre-implantation embryos; and analysing the relationship between cell number and DNA fragmentation with expressions of those genes. The relative abundance of mRNA of 9 pro- (Bax, caspase-9, Bcl-xs, P53, Caspase-3 and Fas) and anti- (Bcl-w and Mcl-1) apoptotic genes was analysed. Differential cell staining and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labelling were performed to analyse the variation in cell numbers and detect apoptotic nuclei respectively. Expression of Bax and Caspase-3 genes was significantly (p < 0.05) higher in poor quality pre-implantation embryos as compared with that of morphologically good quality embryos of the same developmental stages. Moreover, Mcl-1 expression was significantly higher in good quality immature oocytes than that in the poor quality group. Moreover, higher DNA fragmentation was evidenced in morphologically poor quality blastocysts. In conclusion, our study demonstrates that Bax, caspase-3 and Mcl-1 can be used as potential markers of embryo quality to evaluate in vitro-produced bovine embryos. Further studies are required to investigate specific molecular signatures that can be used in evaluating in vivo-derived embryos. |
| doi_str_mv | 10.1111/j.1439-0531.2009.01463.x |
| format | Article |
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The objectives of this study were, therefore, investigating stage-specific expression profiles of apoptosis regulatory genes in three quality groups of in vitro-produced bovine pre-implantation embryos; and analysing the relationship between cell number and DNA fragmentation with expressions of those genes. The relative abundance of mRNA of 9 pro- (Bax, caspase-9, Bcl-xs, P53, Caspase-3 and Fas) and anti- (Bcl-w and Mcl-1) apoptotic genes was analysed. Differential cell staining and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labelling were performed to analyse the variation in cell numbers and detect apoptotic nuclei respectively. Expression of Bax and Caspase-3 genes was significantly (p < 0.05) higher in poor quality pre-implantation embryos as compared with that of morphologically good quality embryos of the same developmental stages. Moreover, Mcl-1 expression was significantly higher in good quality immature oocytes than that in the poor quality group. Moreover, higher DNA fragmentation was evidenced in morphologically poor quality blastocysts. In conclusion, our study demonstrates that Bax, caspase-3 and Mcl-1 can be used as potential markers of embryo quality to evaluate in vitro-produced bovine embryos. Further studies are required to investigate specific molecular signatures that can be used in evaluating in vivo-derived embryos.</description><identifier>ISSN: 0936-6768</identifier><identifier>EISSN: 1439-0531</identifier><identifier>DOI: 10.1111/j.1439-0531.2009.01463.x</identifier><identifier>PMID: 19392665</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Animals ; Apoptosis ; Apoptosis - physiology ; Apoptosis Regulatory Proteins - genetics ; Apoptosis Regulatory Proteins - metabolism ; Bax protein ; Bcl-x protein ; Biological and medical sciences ; blastocysts ; Caspase-3 ; Caspase-9 ; Cattle - embryology ; CD95 antigen ; Cell number ; Developmental stages ; DNA fragmentation ; Embryo, Mammalian - cytology ; Embryo, Mammalian - physiology ; Embryogenesis ; Embryos ; Fas antigen ; Fertilization in Vitro - veterinary ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Gene Expression Regulation, Developmental - physiology ; Genes ; In Situ Nick-End Labeling ; Mammalian reproduction. General aspects ; Mcl-1 protein ; mRNA ; Nuclei ; Oocytes ; p53 protein ; Vertebrates: reproduction</subject><ispartof>Reproduction in domestic animals, 2010-10, Vol.45 (5), p.915-921</ispartof><rights>2009 Blackwell Verlag GmbH</rights><rights>2015 INIST-CNRS</rights><rights>2009 Blackwell Verlag GmbH.</rights><rights>2010 Blackwell Verlag GmbH</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5693-e9e72ea367e106a417be3890e5b47753c4c5eff09f57065d375e783145e04ea83</citedby><cites>FETCH-LOGICAL-c5693-e9e72ea367e106a417be3890e5b47753c4c5eff09f57065d375e783145e04ea83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1439-0531.2009.01463.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1439-0531.2009.01463.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27915,27916,45565,45566</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23218229$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19392665$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Melka, MG</creatorcontrib><creatorcontrib>Rings, F</creatorcontrib><creatorcontrib>Hölker, M</creatorcontrib><creatorcontrib>Tholen, E</creatorcontrib><creatorcontrib>Havlicek, V</creatorcontrib><creatorcontrib>Besenfelder, U</creatorcontrib><creatorcontrib>Schellander, K</creatorcontrib><creatorcontrib>Tesfaye, D</creatorcontrib><title>Expression of Apoptosis Regulatory Genes and Incidence of Apoptosis in Different Morphological Quality Groups of In Vitro-produced Bovine Pre-implantation Embryos</title><title>Reproduction in domestic animals</title><addtitle>Reprod Domest Anim</addtitle><description>Apoptosis occurs during early development in both in vivo- and in vitro-produced embryos, and is considered as one of the causes of embryonic loss. The objectives of this study were, therefore, investigating stage-specific expression profiles of apoptosis regulatory genes in three quality groups of in vitro-produced bovine pre-implantation embryos; and analysing the relationship between cell number and DNA fragmentation with expressions of those genes. The relative abundance of mRNA of 9 pro- (Bax, caspase-9, Bcl-xs, P53, Caspase-3 and Fas) and anti- (Bcl-w and Mcl-1) apoptotic genes was analysed. Differential cell staining and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labelling were performed to analyse the variation in cell numbers and detect apoptotic nuclei respectively. Expression of Bax and Caspase-3 genes was significantly (p < 0.05) higher in poor quality pre-implantation embryos as compared with that of morphologically good quality embryos of the same developmental stages. Moreover, Mcl-1 expression was significantly higher in good quality immature oocytes than that in the poor quality group. Moreover, higher DNA fragmentation was evidenced in morphologically poor quality blastocysts. In conclusion, our study demonstrates that Bax, caspase-3 and Mcl-1 can be used as potential markers of embryo quality to evaluate in vitro-produced bovine embryos. Further studies are required to investigate specific molecular signatures that can be used in evaluating in vivo-derived embryos.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Apoptosis - physiology</subject><subject>Apoptosis Regulatory Proteins - genetics</subject><subject>Apoptosis Regulatory Proteins - metabolism</subject><subject>Bax protein</subject><subject>Bcl-x protein</subject><subject>Biological and medical sciences</subject><subject>blastocysts</subject><subject>Caspase-3</subject><subject>Caspase-9</subject><subject>Cattle - embryology</subject><subject>CD95 antigen</subject><subject>Cell number</subject><subject>Developmental stages</subject><subject>DNA fragmentation</subject><subject>Embryo, Mammalian - cytology</subject><subject>Embryo, Mammalian - physiology</subject><subject>Embryogenesis</subject><subject>Embryos</subject><subject>Fas antigen</subject><subject>Fertilization in Vitro - veterinary</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Developmental - physiology</subject><subject>Genes</subject><subject>In Situ Nick-End Labeling</subject><subject>Mammalian reproduction. General aspects</subject><subject>Mcl-1 protein</subject><subject>mRNA</subject><subject>Nuclei</subject><subject>Oocytes</subject><subject>p53 protein</subject><subject>Vertebrates: reproduction</subject><issn>0936-6768</issn><issn>1439-0531</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcuO0zAYhSMEYoaBVwALCbFKseP4tmBRZkqnUG7DXJaWm_wp7qRxsBNoX4cnxaFVEWzAC9uSv_Pr-JwkQQSPSFwvViOSU5ViRskow1iNMMk5HW3uJMeHh7vJMVaUp1xweZQ8CGGFMWFSiPvJEVFUZZyz4-THZNN6CMG6BrkKjVvXdi7YgC5g2demc36LptBAQKYp0awpbAlNAX-ytkFntqrAQ9Ohd863X1ztlrYwNfrUm9p2cYZ3fRsG2axB17bzLm29K_sCSvTKfbMNoI8eUrtua9N0phv8TNYLv3XhYXKvMnWAR_vzJLl6Pbk8PU_nH6az0_E8LRhXNAUFIgNDuQCCucmJWACVCgNb5EIwWuQFg6rCqmICc1ZSwUBISnIGOAcj6UnyfDc3GvvaQ-j02oYC6mgIXB-0YjmLWXL8TzIyRApMaCSf_kWuXO-b-A0dLUmSS55FSO6gwrsQPFS69XZt_FYTrIe-9UoPteqhVj30rX_1rTdR-ng_v1-sofwt3BccgWd7wITYR-VNrDAcuIxmRGaZitzLHffd1rD9bwP64mw83KI-3elt6GBz0Bt_q7mIUeub91N9nl3K6zdv5_om8k92fGWcNksfPV19zmJkMTkVd0Z_ApPZ390</recordid><startdate>201010</startdate><enddate>201010</enddate><creator>Melka, MG</creator><creator>Rings, F</creator><creator>Hölker, M</creator><creator>Tholen, E</creator><creator>Havlicek, V</creator><creator>Besenfelder, U</creator><creator>Schellander, K</creator><creator>Tesfaye, D</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><scope>RC3</scope></search><sort><creationdate>201010</creationdate><title>Expression of Apoptosis Regulatory Genes and Incidence of Apoptosis in Different Morphological Quality Groups of In Vitro-produced Bovine Pre-implantation Embryos</title><author>Melka, MG ; Rings, F ; Hölker, M ; Tholen, E ; Havlicek, V ; Besenfelder, U ; Schellander, K ; Tesfaye, D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5693-e9e72ea367e106a417be3890e5b47753c4c5eff09f57065d375e783145e04ea83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Apoptosis - physiology</topic><topic>Apoptosis Regulatory Proteins - genetics</topic><topic>Apoptosis Regulatory Proteins - metabolism</topic><topic>Bax protein</topic><topic>Bcl-x protein</topic><topic>Biological and medical sciences</topic><topic>blastocysts</topic><topic>Caspase-3</topic><topic>Caspase-9</topic><topic>Cattle - embryology</topic><topic>CD95 antigen</topic><topic>Cell number</topic><topic>Developmental stages</topic><topic>DNA fragmentation</topic><topic>Embryo, Mammalian - cytology</topic><topic>Embryo, Mammalian - physiology</topic><topic>Embryogenesis</topic><topic>Embryos</topic><topic>Fas antigen</topic><topic>Fertilization in Vitro - veterinary</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Developmental - physiology</topic><topic>Genes</topic><topic>In Situ Nick-End Labeling</topic><topic>Mammalian reproduction. 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The objectives of this study were, therefore, investigating stage-specific expression profiles of apoptosis regulatory genes in three quality groups of in vitro-produced bovine pre-implantation embryos; and analysing the relationship between cell number and DNA fragmentation with expressions of those genes. The relative abundance of mRNA of 9 pro- (Bax, caspase-9, Bcl-xs, P53, Caspase-3 and Fas) and anti- (Bcl-w and Mcl-1) apoptotic genes was analysed. Differential cell staining and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labelling were performed to analyse the variation in cell numbers and detect apoptotic nuclei respectively. Expression of Bax and Caspase-3 genes was significantly (p < 0.05) higher in poor quality pre-implantation embryos as compared with that of morphologically good quality embryos of the same developmental stages. Moreover, Mcl-1 expression was significantly higher in good quality immature oocytes than that in the poor quality group. Moreover, higher DNA fragmentation was evidenced in morphologically poor quality blastocysts. In conclusion, our study demonstrates that Bax, caspase-3 and Mcl-1 can be used as potential markers of embryo quality to evaluate in vitro-produced bovine embryos. Further studies are required to investigate specific molecular signatures that can be used in evaluating in vivo-derived embryos.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>19392665</pmid><doi>10.1111/j.1439-0531.2009.01463.x</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Apoptosis Apoptosis - physiology Apoptosis Regulatory Proteins - genetics Apoptosis Regulatory Proteins - metabolism Bax protein Bcl-x protein Biological and medical sciences blastocysts Caspase-3 Caspase-9 Cattle - embryology CD95 antigen Cell number Developmental stages DNA fragmentation Embryo, Mammalian - cytology Embryo, Mammalian - physiology Embryogenesis Embryos Fas antigen Fertilization in Vitro - veterinary Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation, Developmental - physiology Genes In Situ Nick-End Labeling Mammalian reproduction. General aspects Mcl-1 protein mRNA Nuclei Oocytes p53 protein Vertebrates: reproduction |
| title | Expression of Apoptosis Regulatory Genes and Incidence of Apoptosis in Different Morphological Quality Groups of In Vitro-produced Bovine Pre-implantation Embryos |
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