Separation of monoclonal antibodies from cell-culture supernatants and ascites fluid using thiophilic agarose

A one-step purification procedure will yield monoclonal antibodies from cell-culture supernatants and ascites fluids. The chromatographic adsorbent is thiophilic argose, i.e., beaded agarose gel coupled with ligands of thiophilic nature, often with a sulfone group and a sulfur atom. The chromatograp...

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Veröffentlicht in:	Molecular biotechnology 1997-12, Vol.8 (3), p.279-281
1. Verfasser:	BOG-HANSEN, T. C
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical biochemistry: general aspects, technics, instrumentation Analytical, structural and metabolic biochemistry Antibodies, Monoclonal - isolation & purification Ascites Ascitic Fluid Biological and medical sciences Biotechnology Cell culture Cells, Cultured Culture Media Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Monoclonal antibodies Others Polymers Sepharose Studies Sulfur Various methods and equipments
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Beschreibung
Zusammenfassung:	A one-step purification procedure will yield monoclonal antibodies from cell-culture supernatants and ascites fluids. The chromatographic adsorbent is thiophilic argose, i.e., beaded agarose gel coupled with ligands of thiophilic nature, often with a sulfone group and a sulfur atom. The chromatographic procedure is simply adsorption, wash, elution. The procedure is simple, efficient, and inexpensive.
ISSN:	1073-6085 1559-0305
DOI:	10.1007/BF02760781