Characterization of molecular weight distribution of oligomers from autocatalyzed batch hydrolysis of xylan
Oat spelt xylan was treated with water in a batch reactor at temperaturesof 180 and 200 degrees C. Ion-moderated partition (IMP) chromatography was thenapplied to separate oligomers in solution according to their molecular size. Calibration of the IMP measurements based on peak height was found toqu...
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| Veröffentlicht in: | Applied biochemistry and biotechnology 2003, Vol.105 -108 (1-3), p.515-522 |
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| container_title | Applied biochemistry and biotechnology |
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| creator | Li, Xia Converse, Alvin O Wyman, Charles E |
| description | Oat spelt xylan was treated with water in a batch reactor at temperaturesof 180 and 200 degrees C. Ion-moderated partition (IMP) chromatography was thenapplied to separate oligomers in solution according to their molecular size. Calibration of the IMP measurements based on peak height was found toquantify dissolved monomer and oligomer yields well. Oligomer concentrationsin the liquid hydrolysate were also determined from the difference inxylose monomer concentrations measured by high-performance liquid chromatographybefore and after posthydrolysis of dissolved xylooligosaccharidesto xylose. Delayed formation and then rapid disappearance of oligomersfrom DP10 to DP2 was observed by IMP, and total oligomer yields measuredby IMP and posthydrolysis were very similar at these times. However, whileIMP detected virtually no oligomers initially, posthydrolysis measurementsgave significant amounts of soluble oligomers at these times, indicating thatoligomers with chain lengths >10 were in solution but not detectable by theIMP system used. |
| doi_str_mv | 10.1385/ABAB:107:1-3:515 |
| format | Article |
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Ion-moderated partition (IMP) chromatography was thenapplied to separate oligomers in solution according to their molecular size. Calibration of the IMP measurements based on peak height was found toquantify dissolved monomer and oligomer yields well. Oligomer concentrationsin the liquid hydrolysate were also determined from the difference inxylose monomer concentrations measured by high-performance liquid chromatographybefore and after posthydrolysis of dissolved xylooligosaccharidesto xylose. Delayed formation and then rapid disappearance of oligomersfrom DP10 to DP2 was observed by IMP, and total oligomer yields measuredby IMP and posthydrolysis were very similar at these times. However, whileIMP detected virtually no oligomers initially, posthydrolysis measurementsgave significant amounts of soluble oligomers at these times, indicating thatoligomers with chain lengths >10 were in solution but not detectable by theIMP system used.</description><identifier>ISSN: 0273-2289</identifier><identifier>EISSN: 0273-2289</identifier><identifier>EISSN: 1559-0291</identifier><identifier>DOI: 10.1385/ABAB:107:1-3:515</identifier><identifier>PMID: 12721432</identifier><language>eng</language><publisher>United States: Springer Nature B.V</publisher><subject>Avena - chemistry ; Batch reactors ; Bioreactors ; Chromatography ; Chromatography, Ion Exchange - methods ; Hydrolysis ; Kinetics ; Liquid chromatography ; Molecular Weight ; Oligosaccharides - chemistry ; Oligosaccharides - isolation & purification ; Polymers ; Studies ; Xylans - chemistry ; Xylans - isolation & purification ; Xylose - analysis ; Xylose - isolation & purification</subject><ispartof>Applied biochemistry and biotechnology, 2003, Vol.105 -108 (1-3), p.515-522</ispartof><rights>Humana Press Inc. 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c385t-a997f0dee10481074858c7fc7e5288a647a9cd40614855e143e154fa610719d23</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12721432$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Finkelstein M</contributor><contributor>Davison, BH</contributor><contributor>Lee, JW</contributor><contributor>McMillan, JD</contributor><creatorcontrib>Li, Xia</creatorcontrib><creatorcontrib>Converse, Alvin O</creatorcontrib><creatorcontrib>Wyman, Charles E</creatorcontrib><title>Characterization of molecular weight distribution of oligomers from autocatalyzed batch hydrolysis of xylan</title><title>Applied biochemistry and biotechnology</title><addtitle>Appl Biochem Biotechnol</addtitle><description>Oat spelt xylan was treated with water in a batch reactor at temperaturesof 180 and 200 degrees C. Ion-moderated partition (IMP) chromatography was thenapplied to separate oligomers in solution according to their molecular size. Calibration of the IMP measurements based on peak height was found toquantify dissolved monomer and oligomer yields well. Oligomer concentrationsin the liquid hydrolysate were also determined from the difference inxylose monomer concentrations measured by high-performance liquid chromatographybefore and after posthydrolysis of dissolved xylooligosaccharidesto xylose. Delayed formation and then rapid disappearance of oligomersfrom DP10 to DP2 was observed by IMP, and total oligomer yields measuredby IMP and posthydrolysis were very similar at these times. 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Converse, Alvin O ; Wyman, Charles E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c385t-a997f0dee10481074858c7fc7e5288a647a9cd40614855e143e154fa610719d23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Avena - chemistry</topic><topic>Batch reactors</topic><topic>Bioreactors</topic><topic>Chromatography</topic><topic>Chromatography, Ion Exchange - methods</topic><topic>Hydrolysis</topic><topic>Kinetics</topic><topic>Liquid chromatography</topic><topic>Molecular Weight</topic><topic>Oligosaccharides - chemistry</topic><topic>Oligosaccharides - isolation & purification</topic><topic>Polymers</topic><topic>Studies</topic><topic>Xylans - chemistry</topic><topic>Xylans - isolation & purification</topic><topic>Xylose - analysis</topic><topic>Xylose - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Xia</creatorcontrib><creatorcontrib>Converse, Alvin O</creatorcontrib><creatorcontrib>Wyman, Charles E</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Applied biochemistry and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Xia</au><au>Converse, Alvin O</au><au>Wyman, Charles E</au><au>Finkelstein M</au><au>Davison, BH</au><au>Lee, JW</au><au>McMillan, JD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of molecular weight distribution of oligomers from autocatalyzed batch hydrolysis of xylan</atitle><jtitle>Applied biochemistry and biotechnology</jtitle><addtitle>Appl Biochem Biotechnol</addtitle><date>2003</date><risdate>2003</risdate><volume>105 -108</volume><issue>1-3</issue><spage>515</spage><epage>522</epage><pages>515-522</pages><issn>0273-2289</issn><eissn>0273-2289</eissn><eissn>1559-0291</eissn><abstract>Oat spelt xylan was treated with water in a batch reactor at temperaturesof 180 and 200 degrees C. 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However, whileIMP detected virtually no oligomers initially, posthydrolysis measurementsgave significant amounts of soluble oligomers at these times, indicating thatoligomers with chain lengths >10 were in solution but not detectable by theIMP system used.</abstract><cop>United States</cop><pub>Springer Nature B.V</pub><pmid>12721432</pmid><doi>10.1385/ABAB:107:1-3:515</doi><tpages>8</tpages></addata></record> |
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| subjects | Avena - chemistry Batch reactors Bioreactors Chromatography Chromatography, Ion Exchange - methods Hydrolysis Kinetics Liquid chromatography Molecular Weight Oligosaccharides - chemistry Oligosaccharides - isolation & purification Polymers Studies Xylans - chemistry Xylans - isolation & purification Xylose - analysis Xylose - isolation & purification |
| title | Characterization of molecular weight distribution of oligomers from autocatalyzed batch hydrolysis of xylan |
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