Combined Effects of Hydrogen Sulfide and Lipopolysaccharide on Osteoclast Differentiation in Rats
Background: Lipopolysaccharide (LPS) stimulates osteoclast differentiation through toll‐like receptors (TLRs) 2 and 4, and hydrogen sulfide (H2S) induces osteoclast differentiation. If H2S activates TLRs, H2S may enhance the effects of LPS on osteoclast differentiation. The purpose of the present st...
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| Veröffentlicht in: | Journal of periodontology (1970) 2012-04, Vol.83 (4), p.522-527 |
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| container_title | Journal of periodontology (1970) |
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| creator | Irie, Koichiro Ekuni, Daisuke Tomofuji, Takaaki Endo, Yasumasa Kasuyama, Kenta Yaegaki, Ken Morita, Manabu |
| description | Background: Lipopolysaccharide (LPS) stimulates osteoclast differentiation through toll‐like receptors (TLRs) 2 and 4, and hydrogen sulfide (H2S) induces osteoclast differentiation. If H2S activates TLRs, H2S may enhance the effects of LPS on osteoclast differentiation. The purpose of the present study is to examine the combined effects of sodium hydrogen sulfide (NaHS, an H2S donor drug) and LPS on osteoclast differentiation and TLR expression in rat periodontal tissue.
Methods: Twenty‐eight male Wistar rats (8 weeks old) were divided into four groups (n = 7 per group): a control (no treatment) group and three experimental groups (NaHS group, LPS group, and a combination [NaHS + LPS] group). At 1 day after topical application of NaHS and/or Porphyromonas gingivalis LPS into the gingival sulcus of first molars, the number of tartrate‐resistant acid phosphate (TRAP)‐positive osteoclasts in the periodontal tissue was counted. Expression of TLR2 and TLR4 mRNAs and proteins in the gingival was also assessed.
Results: The number of TRAP‐positive osteoclasts was significantly higher in the combination group than in any other group (P |
| doi_str_mv | 10.1902/jop.2011.110315 |
| format | Article |
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Methods: Twenty‐eight male Wistar rats (8 weeks old) were divided into four groups (n = 7 per group): a control (no treatment) group and three experimental groups (NaHS group, LPS group, and a combination [NaHS + LPS] group). At 1 day after topical application of NaHS and/or Porphyromonas gingivalis LPS into the gingival sulcus of first molars, the number of tartrate‐resistant acid phosphate (TRAP)‐positive osteoclasts in the periodontal tissue was counted. Expression of TLR2 and TLR4 mRNAs and proteins in the gingival was also assessed.
Results: The number of TRAP‐positive osteoclasts was significantly higher in the combination group than in any other group (P <0.01). The combination group had 11.0‐fold higher TLR4 mRNA levels than the control group. TLR4 protein levels were also higher in the combination group than in the NaHS or LPS group. However, the TLR2 mRNA and protein levels were not significantly different in the combination group and the LPS group.
Conclusion: In rat periodontal tissue, NaHS and LPS had an additive effect on osteoclast differentiation through activation of the TLR4 pathway but not the TLR2 pathway.</description><identifier>ISSN: 0022-3492</identifier><identifier>EISSN: 1943-3670</identifier><identifier>DOI: 10.1902/jop.2011.110315</identifier><identifier>PMID: 21910595</identifier><language>eng</language><publisher>Chicago, IL: American Academy of Periodontology</publisher><subject>Acid Phosphatase - analysis ; Animals ; Biological and medical sciences ; Biomarkers - analysis ; Blotting, Western ; Cell Count ; Cell Differentiation - drug effects ; Dentistry ; Gingiva - drug effects ; HMGB1 Protein - drug effects ; Hydrogen sulfide ; Hydrogen Sulfide - pharmacology ; Isoenzymes - analysis ; lipopolysaccharides ; Lipopolysaccharides - pharmacology ; Male ; Medical sciences ; osteoclasts ; Osteoclasts - drug effects ; Otorhinolaryngology. Stomatology ; Periodontium - cytology ; Periodontium - drug effects ; Porphyromonas gingivalis ; RANK Ligand - drug effects ; Rats ; Rats, Wistar ; Tartrate-Resistant Acid Phosphatase ; Toll-Like Receptor 2 - drug effects ; Toll-Like Receptor 4 - drug effects ; toll‐like receptor 2 ; toll‐like receptor 4 ; Tumor Necrosis Factor-alpha - drug effects</subject><ispartof>Journal of periodontology (1970), 2012-04, Vol.83 (4), p.522-527</ispartof><rights>2012 American Academy of Periodontology</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4382-d3d4f487b6c8c1987ad2f57ba29eb806bfb92726883b85395430ef2bf8c0b6313</citedby><cites>FETCH-LOGICAL-c4382-d3d4f487b6c8c1987ad2f57ba29eb806bfb92726883b85395430ef2bf8c0b6313</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1902%2Fjop.2011.110315$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1902%2Fjop.2011.110315$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27929,27930,45579,45580</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25754555$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21910595$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Irie, Koichiro</creatorcontrib><creatorcontrib>Ekuni, Daisuke</creatorcontrib><creatorcontrib>Tomofuji, Takaaki</creatorcontrib><creatorcontrib>Endo, Yasumasa</creatorcontrib><creatorcontrib>Kasuyama, Kenta</creatorcontrib><creatorcontrib>Yaegaki, Ken</creatorcontrib><creatorcontrib>Morita, Manabu</creatorcontrib><title>Combined Effects of Hydrogen Sulfide and Lipopolysaccharide on Osteoclast Differentiation in Rats</title><title>Journal of periodontology (1970)</title><addtitle>J Periodontol</addtitle><description>Background: Lipopolysaccharide (LPS) stimulates osteoclast differentiation through toll‐like receptors (TLRs) 2 and 4, and hydrogen sulfide (H2S) induces osteoclast differentiation. If H2S activates TLRs, H2S may enhance the effects of LPS on osteoclast differentiation. The purpose of the present study is to examine the combined effects of sodium hydrogen sulfide (NaHS, an H2S donor drug) and LPS on osteoclast differentiation and TLR expression in rat periodontal tissue.
Methods: Twenty‐eight male Wistar rats (8 weeks old) were divided into four groups (n = 7 per group): a control (no treatment) group and three experimental groups (NaHS group, LPS group, and a combination [NaHS + LPS] group). At 1 day after topical application of NaHS and/or Porphyromonas gingivalis LPS into the gingival sulcus of first molars, the number of tartrate‐resistant acid phosphate (TRAP)‐positive osteoclasts in the periodontal tissue was counted. Expression of TLR2 and TLR4 mRNAs and proteins in the gingival was also assessed.
Results: The number of TRAP‐positive osteoclasts was significantly higher in the combination group than in any other group (P <0.01). The combination group had 11.0‐fold higher TLR4 mRNA levels than the control group. TLR4 protein levels were also higher in the combination group than in the NaHS or LPS group. However, the TLR2 mRNA and protein levels were not significantly different in the combination group and the LPS group.
Conclusion: In rat periodontal tissue, NaHS and LPS had an additive effect on osteoclast differentiation through activation of the TLR4 pathway but not the TLR2 pathway.</description><subject>Acid Phosphatase - analysis</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - analysis</subject><subject>Blotting, Western</subject><subject>Cell Count</subject><subject>Cell Differentiation - drug effects</subject><subject>Dentistry</subject><subject>Gingiva - drug effects</subject><subject>HMGB1 Protein - drug effects</subject><subject>Hydrogen sulfide</subject><subject>Hydrogen Sulfide - pharmacology</subject><subject>Isoenzymes - analysis</subject><subject>lipopolysaccharides</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>osteoclasts</subject><subject>Osteoclasts - drug effects</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Periodontium - cytology</subject><subject>Periodontium - drug effects</subject><subject>Porphyromonas gingivalis</subject><subject>RANK Ligand - drug effects</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Tartrate-Resistant Acid Phosphatase</subject><subject>Toll-Like Receptor 2 - drug effects</subject><subject>Toll-Like Receptor 4 - drug effects</subject><subject>toll‐like receptor 2</subject><subject>toll‐like receptor 4</subject><subject>Tumor Necrosis Factor-alpha - drug effects</subject><issn>0022-3492</issn><issn>1943-3670</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkLtPwzAQhy0EgvKY2ZAXxJTiZ2KPqBQKqgTiMVu2Y4NRGgc7Fep_T6oWGJms8333O90HwClGYywRufyI3ZggjMcYI4r5DhhhyWhBywrtghFChBSUSXIADnP-GErMKNoHBwRLjLjkI6AncWFC62o49d7ZPsPo4WxVp_jmWvi8bHyoHdRtDeehi11sVllb-67T-ju28CH3LtpG5x5ehyEhubYPug9DK7TwSff5GOx53WR3sn2PwOvN9GUyK-YPt3eTq3lhGRWkqGnNPBOVKa2wWIpK18TzymginRGoNN5IUpFSCGoEp5IPlzhPjBcWmZJiegQuNrldip9Ll3u1CNm6ptGti8usJBMSSVahgbzckDbFnJPzqkthodNKYaTWWtWgVa21qo3WYeJsm700C1f_8j8eB-B8C-hsdeOTbm3IfxyvOON8zfEN9xUat_pvr7p_nD4hTgj9BooIkKo</recordid><startdate>201204</startdate><enddate>201204</enddate><creator>Irie, Koichiro</creator><creator>Ekuni, Daisuke</creator><creator>Tomofuji, Takaaki</creator><creator>Endo, Yasumasa</creator><creator>Kasuyama, Kenta</creator><creator>Yaegaki, Ken</creator><creator>Morita, Manabu</creator><general>American Academy of Periodontology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201204</creationdate><title>Combined Effects of Hydrogen Sulfide and Lipopolysaccharide on Osteoclast Differentiation in Rats</title><author>Irie, Koichiro ; Ekuni, Daisuke ; Tomofuji, Takaaki ; Endo, Yasumasa ; Kasuyama, Kenta ; Yaegaki, Ken ; Morita, Manabu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4382-d3d4f487b6c8c1987ad2f57ba29eb806bfb92726883b85395430ef2bf8c0b6313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Acid Phosphatase - analysis</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biomarkers - analysis</topic><topic>Blotting, Western</topic><topic>Cell Count</topic><topic>Cell Differentiation - drug effects</topic><topic>Dentistry</topic><topic>Gingiva - drug effects</topic><topic>HMGB1 Protein - drug effects</topic><topic>Hydrogen sulfide</topic><topic>Hydrogen Sulfide - pharmacology</topic><topic>Isoenzymes - analysis</topic><topic>lipopolysaccharides</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>osteoclasts</topic><topic>Osteoclasts - drug effects</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>Periodontium - cytology</topic><topic>Periodontium - drug effects</topic><topic>Porphyromonas gingivalis</topic><topic>RANK Ligand - drug effects</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Tartrate-Resistant Acid Phosphatase</topic><topic>Toll-Like Receptor 2 - drug effects</topic><topic>Toll-Like Receptor 4 - drug effects</topic><topic>toll‐like receptor 2</topic><topic>toll‐like receptor 4</topic><topic>Tumor Necrosis Factor-alpha - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Irie, Koichiro</creatorcontrib><creatorcontrib>Ekuni, Daisuke</creatorcontrib><creatorcontrib>Tomofuji, Takaaki</creatorcontrib><creatorcontrib>Endo, Yasumasa</creatorcontrib><creatorcontrib>Kasuyama, Kenta</creatorcontrib><creatorcontrib>Yaegaki, Ken</creatorcontrib><creatorcontrib>Morita, Manabu</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of periodontology (1970)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Irie, Koichiro</au><au>Ekuni, Daisuke</au><au>Tomofuji, Takaaki</au><au>Endo, Yasumasa</au><au>Kasuyama, Kenta</au><au>Yaegaki, Ken</au><au>Morita, Manabu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Combined Effects of Hydrogen Sulfide and Lipopolysaccharide on Osteoclast Differentiation in Rats</atitle><jtitle>Journal of periodontology (1970)</jtitle><addtitle>J Periodontol</addtitle><date>2012-04</date><risdate>2012</risdate><volume>83</volume><issue>4</issue><spage>522</spage><epage>527</epage><pages>522-527</pages><issn>0022-3492</issn><eissn>1943-3670</eissn><abstract>Background: Lipopolysaccharide (LPS) stimulates osteoclast differentiation through toll‐like receptors (TLRs) 2 and 4, and hydrogen sulfide (H2S) induces osteoclast differentiation. If H2S activates TLRs, H2S may enhance the effects of LPS on osteoclast differentiation. The purpose of the present study is to examine the combined effects of sodium hydrogen sulfide (NaHS, an H2S donor drug) and LPS on osteoclast differentiation and TLR expression in rat periodontal tissue.
Methods: Twenty‐eight male Wistar rats (8 weeks old) were divided into four groups (n = 7 per group): a control (no treatment) group and three experimental groups (NaHS group, LPS group, and a combination [NaHS + LPS] group). At 1 day after topical application of NaHS and/or Porphyromonas gingivalis LPS into the gingival sulcus of first molars, the number of tartrate‐resistant acid phosphate (TRAP)‐positive osteoclasts in the periodontal tissue was counted. Expression of TLR2 and TLR4 mRNAs and proteins in the gingival was also assessed.
Results: The number of TRAP‐positive osteoclasts was significantly higher in the combination group than in any other group (P <0.01). The combination group had 11.0‐fold higher TLR4 mRNA levels than the control group. TLR4 protein levels were also higher in the combination group than in the NaHS or LPS group. However, the TLR2 mRNA and protein levels were not significantly different in the combination group and the LPS group.
Conclusion: In rat periodontal tissue, NaHS and LPS had an additive effect on osteoclast differentiation through activation of the TLR4 pathway but not the TLR2 pathway.</abstract><cop>Chicago, IL</cop><pub>American Academy of Periodontology</pub><pmid>21910595</pmid><doi>10.1902/jop.2011.110315</doi><tpages>6</tpages></addata></record> |
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| subjects | Acid Phosphatase - analysis Animals Biological and medical sciences Biomarkers - analysis Blotting, Western Cell Count Cell Differentiation - drug effects Dentistry Gingiva - drug effects HMGB1 Protein - drug effects Hydrogen sulfide Hydrogen Sulfide - pharmacology Isoenzymes - analysis lipopolysaccharides Lipopolysaccharides - pharmacology Male Medical sciences osteoclasts Osteoclasts - drug effects Otorhinolaryngology. Stomatology Periodontium - cytology Periodontium - drug effects Porphyromonas gingivalis RANK Ligand - drug effects Rats Rats, Wistar Tartrate-Resistant Acid Phosphatase Toll-Like Receptor 2 - drug effects Toll-Like Receptor 4 - drug effects toll‐like receptor 2 toll‐like receptor 4 Tumor Necrosis Factor-alpha - drug effects |
| title | Combined Effects of Hydrogen Sulfide and Lipopolysaccharide on Osteoclast Differentiation in Rats |
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