Spectral characteristics, DNA-binding and cytotoxicity of two functional Ru(ii) mixed-ligand complexes
Two functional Ru( ii ) mixed-ligand complexes, [Ru(phen) 2 (ttbd)] 2+ ( 1 ) (ttbd = 4-(6-propenyl-pyrido[3,2- a ]phenzain-10-yl-benzene-1,2-diamine, phen = 1,10-phenanthroline) and [Ru(bpy) 2 (ttbd)] 2+ ( 2 ) (bpy = 2,2-bipyridine), have been synthesized and characterized. The spectral characterist...
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| Veröffentlicht in: | Dalton transactions : an international journal of inorganic chemistry 2012-04, Vol.41 (15), p.4575-4587 |
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| creator | Tan, Lifeng Shen, Jianliang Liu, Jing Zeng, Leli Jin, Lianhe Weng, Chao |
| description | Two functional Ru(
ii
) mixed-ligand complexes, [Ru(phen)
2
(ttbd)]
2+
(
1
) (ttbd = 4-(6-propenyl-pyrido[3,2-
a
]phenzain-10-yl-benzene-1,2-diamine, phen = 1,10-phenanthroline) and [Ru(bpy)
2
(ttbd)]
2+
(
2
) (bpy = 2,2-bipyridine), have been synthesized and characterized. The spectral characteristics of complexes
1
and
2
were investigated using fluorescence spectroscopy and revealed that both complexes were very sensitive to solvent polarity and oxygen molecules in nonaqueous solvents. The binding properties of the two complexes towards calf thymus DNA (CT-DNA) were investigated with different spectrophotometric methods, viscosity measurements and quantum chemistry calculations, indicating that both complexes could enantioselectively bind to CT-DNA by means of intercalation, but with different binding strengths and discrimination. On the other hand, the cytotoxicity of both complexes have been evaluated by MTT assays and Giemsa staining experiments. The main results reveal that the hydrophobicity and surface area of the ancillary ligands have a significant effect on their DNA binding behavior and both complexes are likely to be useful for optically probing nonaqueous and oxygen-free environments.
Two functional Ru(
ii
) mixed-ligand complexes, [Ru(phen)
2
(ttbd)]
2+
(
1
) (ttbd = 4-(6-propenyl-pyrido[3,2-
a
]phenzain-10-yl-benzene-1,2-diamine, phen = 1,10-phenanthroline) and [Ru(bpy)
2
(ttbd)]
2+
(
2
) (bpy = 2,2-bipyridine), have been synthesized and characterized. |
| doi_str_mv | 10.1039/c2dt12402e |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_934263593</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>934263593</sourcerecordid><originalsourceid>FETCH-LOGICAL-c466t-d355607c8b31678794f812de8dfe330345af11e97a5ea0391c935005adb32ea43</originalsourceid><addsrcrecordid>eNqF0Utv3CAUBWBUtWpe3XSfyF0lreIWuAbsZZRHWylKpD7WFgOXhMg2DmB15t_H6aSTXboCie8eQIeQ94x-ZhSaL4bbzHhFOb4i26xSqmw4VK83ey63yE5Kd5RyTgV_S7Y4B1FDLbeJ-zmiyVF3hbnVUZuM0afsTTouzq5OyoUfrB9uCj3YwqxyyGHpjc-rIrgi_wmFmwaTfRjm-R_Tkfcfi94v0Zadv_k7EvqxwyWmPfLG6S7hu6d1l_y-OP91-q28vP76_fTksjSVlLm0IISkytQLYFLVqqlczbjF2joEoFAJ7RjDRmmBev46Mw0ISoW2C-CoK9glh-vcMYb7CVNue58Mdp0eMEypbaDiEkQDszx6UTIpGCjVKPl_Or-ZsVqIx_s_ramJIaWIrh2j73VctYy2j2W1z2XN-OApd1r0aDf0Xzsz-LAGMZnN6XNAO1o3m_2XDDwAJfmkDw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1560118554</pqid></control><display><type>article</type><title>Spectral characteristics, DNA-binding and cytotoxicity of two functional Ru(ii) mixed-ligand complexes</title><source>MEDLINE</source><source>Royal Society Of Chemistry Journals 2008-</source><source>Alma/SFX Local Collection</source><creator>Tan, Lifeng ; Shen, Jianliang ; Liu, Jing ; Zeng, Leli ; Jin, Lianhe ; Weng, Chao</creator><creatorcontrib>Tan, Lifeng ; Shen, Jianliang ; Liu, Jing ; Zeng, Leli ; Jin, Lianhe ; Weng, Chao</creatorcontrib><description>Two functional Ru(
ii
) mixed-ligand complexes, [Ru(phen)
2
(ttbd)]
2+
(
1
) (ttbd = 4-(6-propenyl-pyrido[3,2-
a
]phenzain-10-yl-benzene-1,2-diamine, phen = 1,10-phenanthroline) and [Ru(bpy)
2
(ttbd)]
2+
(
2
) (bpy = 2,2-bipyridine), have been synthesized and characterized. The spectral characteristics of complexes
1
and
2
were investigated using fluorescence spectroscopy and revealed that both complexes were very sensitive to solvent polarity and oxygen molecules in nonaqueous solvents. The binding properties of the two complexes towards calf thymus DNA (CT-DNA) were investigated with different spectrophotometric methods, viscosity measurements and quantum chemistry calculations, indicating that both complexes could enantioselectively bind to CT-DNA by means of intercalation, but with different binding strengths and discrimination. On the other hand, the cytotoxicity of both complexes have been evaluated by MTT assays and Giemsa staining experiments. The main results reveal that the hydrophobicity and surface area of the ancillary ligands have a significant effect on their DNA binding behavior and both complexes are likely to be useful for optically probing nonaqueous and oxygen-free environments.
Two functional Ru(
ii
) mixed-ligand complexes, [Ru(phen)
2
(ttbd)]
2+
(
1
) (ttbd = 4-(6-propenyl-pyrido[3,2-
a
]phenzain-10-yl-benzene-1,2-diamine, phen = 1,10-phenanthroline) and [Ru(bpy)
2
(ttbd)]
2+
(
2
) (bpy = 2,2-bipyridine), have been synthesized and characterized.</description><identifier>ISSN: 1477-9226</identifier><identifier>EISSN: 1477-9234</identifier><identifier>DOI: 10.1039/c2dt12402e</identifier><identifier>PMID: 22358386</identifier><language>eng</language><publisher>England</publisher><subject>2,2'-Dipyridyl - chemistry ; 2,2'-Dipyridyl - pharmacology ; Animals ; Antineoplastic Agents - chemistry ; Antineoplastic Agents - pharmacology ; Binding ; Cattle ; Cell Survival - drug effects ; Coordination Complexes - chemistry ; Coordination Complexes - pharmacology ; Deoxyribonucleic acid ; DNA - metabolism ; Fluorescence ; HeLa Cells ; Hep G2 Cells ; Humans ; Hydrophobicity ; Ligands ; Models, Molecular ; Neoplasms - drug therapy ; Phenanthrolines - chemistry ; Phenanthrolines - pharmacology ; Quantum chemistry ; Ruthenium - chemistry ; Ruthenium - pharmacology ; Solvents ; Spectra ; Spectrum Analysis ; Staining</subject><ispartof>Dalton transactions : an international journal of inorganic chemistry, 2012-04, Vol.41 (15), p.4575-4587</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c466t-d355607c8b31678794f812de8dfe330345af11e97a5ea0391c935005adb32ea43</citedby><cites>FETCH-LOGICAL-c466t-d355607c8b31678794f812de8dfe330345af11e97a5ea0391c935005adb32ea43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22358386$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tan, Lifeng</creatorcontrib><creatorcontrib>Shen, Jianliang</creatorcontrib><creatorcontrib>Liu, Jing</creatorcontrib><creatorcontrib>Zeng, Leli</creatorcontrib><creatorcontrib>Jin, Lianhe</creatorcontrib><creatorcontrib>Weng, Chao</creatorcontrib><title>Spectral characteristics, DNA-binding and cytotoxicity of two functional Ru(ii) mixed-ligand complexes</title><title>Dalton transactions : an international journal of inorganic chemistry</title><addtitle>Dalton Trans</addtitle><description>Two functional Ru(
ii
) mixed-ligand complexes, [Ru(phen)
2
(ttbd)]
2+
(
1
) (ttbd = 4-(6-propenyl-pyrido[3,2-
a
]phenzain-10-yl-benzene-1,2-diamine, phen = 1,10-phenanthroline) and [Ru(bpy)
2
(ttbd)]
2+
(
2
) (bpy = 2,2-bipyridine), have been synthesized and characterized. The spectral characteristics of complexes
1
and
2
were investigated using fluorescence spectroscopy and revealed that both complexes were very sensitive to solvent polarity and oxygen molecules in nonaqueous solvents. The binding properties of the two complexes towards calf thymus DNA (CT-DNA) were investigated with different spectrophotometric methods, viscosity measurements and quantum chemistry calculations, indicating that both complexes could enantioselectively bind to CT-DNA by means of intercalation, but with different binding strengths and discrimination. On the other hand, the cytotoxicity of both complexes have been evaluated by MTT assays and Giemsa staining experiments. The main results reveal that the hydrophobicity and surface area of the ancillary ligands have a significant effect on their DNA binding behavior and both complexes are likely to be useful for optically probing nonaqueous and oxygen-free environments.
Two functional Ru(
ii
) mixed-ligand complexes, [Ru(phen)
2
(ttbd)]
2+
(
1
) (ttbd = 4-(6-propenyl-pyrido[3,2-
a
]phenzain-10-yl-benzene-1,2-diamine, phen = 1,10-phenanthroline) and [Ru(bpy)
2
(ttbd)]
2+
(
2
) (bpy = 2,2-bipyridine), have been synthesized and characterized.</description><subject>2,2'-Dipyridyl - chemistry</subject><subject>2,2'-Dipyridyl - pharmacology</subject><subject>Animals</subject><subject>Antineoplastic Agents - chemistry</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Binding</subject><subject>Cattle</subject><subject>Cell Survival - drug effects</subject><subject>Coordination Complexes - chemistry</subject><subject>Coordination Complexes - pharmacology</subject><subject>Deoxyribonucleic acid</subject><subject>DNA - metabolism</subject><subject>Fluorescence</subject><subject>HeLa Cells</subject><subject>Hep G2 Cells</subject><subject>Humans</subject><subject>Hydrophobicity</subject><subject>Ligands</subject><subject>Models, Molecular</subject><subject>Neoplasms - drug therapy</subject><subject>Phenanthrolines - chemistry</subject><subject>Phenanthrolines - pharmacology</subject><subject>Quantum chemistry</subject><subject>Ruthenium - chemistry</subject><subject>Ruthenium - pharmacology</subject><subject>Solvents</subject><subject>Spectra</subject><subject>Spectrum Analysis</subject><subject>Staining</subject><issn>1477-9226</issn><issn>1477-9234</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0Utv3CAUBWBUtWpe3XSfyF0lreIWuAbsZZRHWylKpD7WFgOXhMg2DmB15t_H6aSTXboCie8eQIeQ94x-ZhSaL4bbzHhFOb4i26xSqmw4VK83ey63yE5Kd5RyTgV_S7Y4B1FDLbeJ-zmiyVF3hbnVUZuM0afsTTouzq5OyoUfrB9uCj3YwqxyyGHpjc-rIrgi_wmFmwaTfRjm-R_Tkfcfi94v0Zadv_k7EvqxwyWmPfLG6S7hu6d1l_y-OP91-q28vP76_fTksjSVlLm0IISkytQLYFLVqqlczbjF2joEoFAJ7RjDRmmBev46Mw0ISoW2C-CoK9glh-vcMYb7CVNue58Mdp0eMEypbaDiEkQDszx6UTIpGCjVKPl_Or-ZsVqIx_s_ramJIaWIrh2j73VctYy2j2W1z2XN-OApd1r0aDf0Xzsz-LAGMZnN6XNAO1o3m_2XDDwAJfmkDw</recordid><startdate>20120421</startdate><enddate>20120421</enddate><creator>Tan, Lifeng</creator><creator>Shen, Jianliang</creator><creator>Liu, Jing</creator><creator>Zeng, Leli</creator><creator>Jin, Lianhe</creator><creator>Weng, Chao</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20120421</creationdate><title>Spectral characteristics, DNA-binding and cytotoxicity of two functional Ru(ii) mixed-ligand complexes</title><author>Tan, Lifeng ; Shen, Jianliang ; Liu, Jing ; Zeng, Leli ; Jin, Lianhe ; Weng, Chao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c466t-d355607c8b31678794f812de8dfe330345af11e97a5ea0391c935005adb32ea43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>2,2'-Dipyridyl - chemistry</topic><topic>2,2'-Dipyridyl - pharmacology</topic><topic>Animals</topic><topic>Antineoplastic Agents - chemistry</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Binding</topic><topic>Cattle</topic><topic>Cell Survival - drug effects</topic><topic>Coordination Complexes - chemistry</topic><topic>Coordination Complexes - pharmacology</topic><topic>Deoxyribonucleic acid</topic><topic>DNA - metabolism</topic><topic>Fluorescence</topic><topic>HeLa Cells</topic><topic>Hep G2 Cells</topic><topic>Humans</topic><topic>Hydrophobicity</topic><topic>Ligands</topic><topic>Models, Molecular</topic><topic>Neoplasms - drug therapy</topic><topic>Phenanthrolines - chemistry</topic><topic>Phenanthrolines - pharmacology</topic><topic>Quantum chemistry</topic><topic>Ruthenium - chemistry</topic><topic>Ruthenium - pharmacology</topic><topic>Solvents</topic><topic>Spectra</topic><topic>Spectrum Analysis</topic><topic>Staining</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tan, Lifeng</creatorcontrib><creatorcontrib>Shen, Jianliang</creatorcontrib><creatorcontrib>Liu, Jing</creatorcontrib><creatorcontrib>Zeng, Leli</creatorcontrib><creatorcontrib>Jin, Lianhe</creatorcontrib><creatorcontrib>Weng, Chao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Dalton transactions : an international journal of inorganic chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tan, Lifeng</au><au>Shen, Jianliang</au><au>Liu, Jing</au><au>Zeng, Leli</au><au>Jin, Lianhe</au><au>Weng, Chao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Spectral characteristics, DNA-binding and cytotoxicity of two functional Ru(ii) mixed-ligand complexes</atitle><jtitle>Dalton transactions : an international journal of inorganic chemistry</jtitle><addtitle>Dalton Trans</addtitle><date>2012-04-21</date><risdate>2012</risdate><volume>41</volume><issue>15</issue><spage>4575</spage><epage>4587</epage><pages>4575-4587</pages><issn>1477-9226</issn><eissn>1477-9234</eissn><abstract>Two functional Ru(
ii
) mixed-ligand complexes, [Ru(phen)
2
(ttbd)]
2+
(
1
) (ttbd = 4-(6-propenyl-pyrido[3,2-
a
]phenzain-10-yl-benzene-1,2-diamine, phen = 1,10-phenanthroline) and [Ru(bpy)
2
(ttbd)]
2+
(
2
) (bpy = 2,2-bipyridine), have been synthesized and characterized. The spectral characteristics of complexes
1
and
2
were investigated using fluorescence spectroscopy and revealed that both complexes were very sensitive to solvent polarity and oxygen molecules in nonaqueous solvents. The binding properties of the two complexes towards calf thymus DNA (CT-DNA) were investigated with different spectrophotometric methods, viscosity measurements and quantum chemistry calculations, indicating that both complexes could enantioselectively bind to CT-DNA by means of intercalation, but with different binding strengths and discrimination. On the other hand, the cytotoxicity of both complexes have been evaluated by MTT assays and Giemsa staining experiments. The main results reveal that the hydrophobicity and surface area of the ancillary ligands have a significant effect on their DNA binding behavior and both complexes are likely to be useful for optically probing nonaqueous and oxygen-free environments.
Two functional Ru(
ii
) mixed-ligand complexes, [Ru(phen)
2
(ttbd)]
2+
(
1
) (ttbd = 4-(6-propenyl-pyrido[3,2-
a
]phenzain-10-yl-benzene-1,2-diamine, phen = 1,10-phenanthroline) and [Ru(bpy)
2
(ttbd)]
2+
(
2
) (bpy = 2,2-bipyridine), have been synthesized and characterized.</abstract><cop>England</cop><pmid>22358386</pmid><doi>10.1039/c2dt12402e</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1477-9226 |
| ispartof | Dalton transactions : an international journal of inorganic chemistry, 2012-04, Vol.41 (15), p.4575-4587 |
| issn | 1477-9226 1477-9234 |
| language | eng |
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| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | 2,2'-Dipyridyl - chemistry 2,2'-Dipyridyl - pharmacology Animals Antineoplastic Agents - chemistry Antineoplastic Agents - pharmacology Binding Cattle Cell Survival - drug effects Coordination Complexes - chemistry Coordination Complexes - pharmacology Deoxyribonucleic acid DNA - metabolism Fluorescence HeLa Cells Hep G2 Cells Humans Hydrophobicity Ligands Models, Molecular Neoplasms - drug therapy Phenanthrolines - chemistry Phenanthrolines - pharmacology Quantum chemistry Ruthenium - chemistry Ruthenium - pharmacology Solvents Spectra Spectrum Analysis Staining |
| title | Spectral characteristics, DNA-binding and cytotoxicity of two functional Ru(ii) mixed-ligand complexes |
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