Antibacterial substantivity of a new antibiotic-based endodontic irrigation solution
The aim of this study was to evaluate the residual antibacterial activity of Tetraclean, MTAD and 5.25% sodium hypochlorite (NaOCl) in bovine root dentin in vitro. One hundred and ten dentin tubes prepared from bovine incisor teeth were infected in vitro for 14 days with Enterococcus faecalis. There...
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Veröffentlicht in: | Australian endodontic journal 2012-04, Vol.38 (1), p.26-30 |
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description | The aim of this study was to evaluate the residual antibacterial activity of Tetraclean, MTAD and 5.25% sodium hypochlorite (NaOCl) in bovine root dentin in vitro. One hundred and ten dentin tubes prepared from bovine incisor teeth were infected in vitro for 14 days with Enterococcus faecalis. Thereafter, the specimens were divided into five groups as follows: Tetraclean; MTAD; 5.25% NaOCl; infected dentin tubes (positive control); and sterile dentin tubes (negative control). Dentin chips were collected using round burs into tryptic soy broth and after culturing, the number of colony‐forming units (CFU) was counted. The number of CFU in all experimental groups was minimum after treatment, and the results obtained were significantly different from each other at any time period (P |
doi_str_mv | 10.1111/j.1747-4477.2010.00263.x |
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One hundred and ten dentin tubes prepared from bovine incisor teeth were infected in vitro for 14 days with Enterococcus faecalis. Thereafter, the specimens were divided into five groups as follows: Tetraclean; MTAD; 5.25% NaOCl; infected dentin tubes (positive control); and sterile dentin tubes (negative control). Dentin chips were collected using round burs into tryptic soy broth and after culturing, the number of colony‐forming units (CFU) was counted. The number of CFU in all experimental groups was minimum after treatment, and the results obtained were significantly different from each other at any time period (P < 0.05). The Tetraclean group showed the most effective antibacterial action at all five experimental periods (P < 0.05). MTAD group showed the least antibacterial activity after treatment. However, at days 7, 14, 21 and 28 MTAD showed more effective antibacterial action than NaOCl. In each group, the number of CFU increased significantly by time‐lapse (P < 0.05). In conclusion, the residual antibacterial activity of Tetraclean was significantly greater than MTAD and 5.25% NaOCl.</description><identifier>ISSN: 1329-1947</identifier><identifier>EISSN: 1747-4477</identifier><identifier>DOI: 10.1111/j.1747-4477.2010.00263.x</identifier><identifier>PMID: 22432823</identifier><language>eng</language><publisher>Melbourne, Australia: Blackwell Publishing Asia</publisher><subject>Analysis of Variance ; Animals ; Anti-Bacterial Agents - pharmacology ; Cattle ; Colony Count, Microbial ; Dental Pulp Cavity - microbiology ; Dentin ; Dentin - microbiology ; Dentistry ; doxycycline ; Enterococcus faecalis ; Enterococcus faecalis - drug effects ; Enterococcus faecalis - isolation & purification ; Food science ; In Vitro Techniques ; Microbiology ; MTAD ; Root Canal Irrigants - pharmacology ; substantivity ; Tetraclean</subject><ispartof>Australian endodontic journal, 2012-04, Vol.38 (1), p.26-30</ispartof><rights>2010 The Authors. Australian Endodontic Journal © 2010 Australian Society of Endodontology</rights><rights>2010 The Authors. Australian Endodontic Journal © 2010 Australian Society of Endodontology.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4993-c3efe3ffce5f89c5eaa93ab4e62f7b8eea536a275da69a3828af46fde98674d33</citedby><cites>FETCH-LOGICAL-c4993-c3efe3ffce5f89c5eaa93ab4e62f7b8eea536a275da69a3828af46fde98674d33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1747-4477.2010.00263.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1747-4477.2010.00263.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22432823$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mohammadi, Zahed</creatorcontrib><creatorcontrib>Giardino, Luciano</creatorcontrib><creatorcontrib>Mombeinipour, Ali</creatorcontrib><title>Antibacterial substantivity of a new antibiotic-based endodontic irrigation solution</title><title>Australian endodontic journal</title><addtitle>Aust Endod J</addtitle><description>The aim of this study was to evaluate the residual antibacterial activity of Tetraclean, MTAD and 5.25% sodium hypochlorite (NaOCl) in bovine root dentin in vitro. One hundred and ten dentin tubes prepared from bovine incisor teeth were infected in vitro for 14 days with Enterococcus faecalis. Thereafter, the specimens were divided into five groups as follows: Tetraclean; MTAD; 5.25% NaOCl; infected dentin tubes (positive control); and sterile dentin tubes (negative control). Dentin chips were collected using round burs into tryptic soy broth and after culturing, the number of colony‐forming units (CFU) was counted. The number of CFU in all experimental groups was minimum after treatment, and the results obtained were significantly different from each other at any time period (P < 0.05). The Tetraclean group showed the most effective antibacterial action at all five experimental periods (P < 0.05). MTAD group showed the least antibacterial activity after treatment. However, at days 7, 14, 21 and 28 MTAD showed more effective antibacterial action than NaOCl. In each group, the number of CFU increased significantly by time‐lapse (P < 0.05). In conclusion, the residual antibacterial activity of Tetraclean was significantly greater than MTAD and 5.25% NaOCl.</description><subject>Analysis of Variance</subject><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Cattle</subject><subject>Colony Count, Microbial</subject><subject>Dental Pulp Cavity - microbiology</subject><subject>Dentin</subject><subject>Dentin - microbiology</subject><subject>Dentistry</subject><subject>doxycycline</subject><subject>Enterococcus faecalis</subject><subject>Enterococcus faecalis - drug effects</subject><subject>Enterococcus faecalis - isolation & purification</subject><subject>Food science</subject><subject>In Vitro Techniques</subject><subject>Microbiology</subject><subject>MTAD</subject><subject>Root Canal Irrigants - pharmacology</subject><subject>substantivity</subject><subject>Tetraclean</subject><issn>1329-1947</issn><issn>1747-4477</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkM1u1DAUhS0EoqXwCsgSC1aZxj-xY4nNaNQOlFFZUAQ76ya5Rh4ycbETOvP2OEyZBSu88dHxOfdaHyGUlQuWz-V2wbTUhZRaL3iZ3bLkSiz2T8j56eFp1oKbghmpz8iLlLY5JIVmz8kZz4LXXJyTu-Uw-gbaEaOHnqapSSNk65cfDzQ4CnTABzo7jQ-jb4sGEnYUhy50Ibst9TH67zD6MNAU-mkWL8kzB33CV4_3BflyfXW3el9sPq0_rJabopXGiKIV6FA412LlatNWCGAENBIVd7qpEaESCriuOlAGRM1rcFK5Dk2ttOyEuCBvj3PvY_g5YRrtzqcW-x4GDFOyRkiuOGM6J9_8k9yGKQ75czZDYlKrSrGcqo-pNoaUIjp7H_0O4sGy0s7g7dbOfO3M187g7R_wdp-rrx8XTM0Ou1PxL-kceHcMPPgeD_892C6vbrLI9eJY92nE_akO8YdVWujKfr1d25X8-HltNrf2m_gNEmGiAQ</recordid><startdate>201204</startdate><enddate>201204</enddate><creator>Mohammadi, Zahed</creator><creator>Giardino, Luciano</creator><creator>Mombeinipour, Ali</creator><general>Blackwell Publishing Asia</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>201204</creationdate><title>Antibacterial substantivity of a new antibiotic-based endodontic irrigation solution</title><author>Mohammadi, Zahed ; Giardino, Luciano ; Mombeinipour, Ali</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4993-c3efe3ffce5f89c5eaa93ab4e62f7b8eea536a275da69a3828af46fde98674d33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Analysis of Variance</topic><topic>Animals</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Cattle</topic><topic>Colony Count, Microbial</topic><topic>Dental Pulp Cavity - microbiology</topic><topic>Dentin</topic><topic>Dentin - microbiology</topic><topic>Dentistry</topic><topic>doxycycline</topic><topic>Enterococcus faecalis</topic><topic>Enterococcus faecalis - drug effects</topic><topic>Enterococcus faecalis - isolation & purification</topic><topic>Food science</topic><topic>In Vitro Techniques</topic><topic>Microbiology</topic><topic>MTAD</topic><topic>Root Canal Irrigants - pharmacology</topic><topic>substantivity</topic><topic>Tetraclean</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mohammadi, Zahed</creatorcontrib><creatorcontrib>Giardino, Luciano</creatorcontrib><creatorcontrib>Mombeinipour, Ali</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Australian endodontic journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mohammadi, Zahed</au><au>Giardino, Luciano</au><au>Mombeinipour, Ali</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antibacterial substantivity of a new antibiotic-based endodontic irrigation solution</atitle><jtitle>Australian endodontic journal</jtitle><addtitle>Aust Endod J</addtitle><date>2012-04</date><risdate>2012</risdate><volume>38</volume><issue>1</issue><spage>26</spage><epage>30</epage><pages>26-30</pages><issn>1329-1947</issn><eissn>1747-4477</eissn><abstract>The aim of this study was to evaluate the residual antibacterial activity of Tetraclean, MTAD and 5.25% sodium hypochlorite (NaOCl) in bovine root dentin in vitro. One hundred and ten dentin tubes prepared from bovine incisor teeth were infected in vitro for 14 days with Enterococcus faecalis. Thereafter, the specimens were divided into five groups as follows: Tetraclean; MTAD; 5.25% NaOCl; infected dentin tubes (positive control); and sterile dentin tubes (negative control). Dentin chips were collected using round burs into tryptic soy broth and after culturing, the number of colony‐forming units (CFU) was counted. The number of CFU in all experimental groups was minimum after treatment, and the results obtained were significantly different from each other at any time period (P < 0.05). The Tetraclean group showed the most effective antibacterial action at all five experimental periods (P < 0.05). MTAD group showed the least antibacterial activity after treatment. However, at days 7, 14, 21 and 28 MTAD showed more effective antibacterial action than NaOCl. In each group, the number of CFU increased significantly by time‐lapse (P < 0.05). In conclusion, the residual antibacterial activity of Tetraclean was significantly greater than MTAD and 5.25% NaOCl.</abstract><cop>Melbourne, Australia</cop><pub>Blackwell Publishing Asia</pub><pmid>22432823</pmid><doi>10.1111/j.1747-4477.2010.00263.x</doi><tpages>5</tpages></addata></record> |
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subjects | Analysis of Variance Animals Anti-Bacterial Agents - pharmacology Cattle Colony Count, Microbial Dental Pulp Cavity - microbiology Dentin Dentin - microbiology Dentistry doxycycline Enterococcus faecalis Enterococcus faecalis - drug effects Enterococcus faecalis - isolation & purification Food science In Vitro Techniques Microbiology MTAD Root Canal Irrigants - pharmacology substantivity Tetraclean |
title | Antibacterial substantivity of a new antibiotic-based endodontic irrigation solution |
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