Antibacterial substantivity of a new antibiotic-based endodontic irrigation solution

The aim of this study was to evaluate the residual antibacterial activity of Tetraclean, MTAD and 5.25% sodium hypochlorite (NaOCl) in bovine root dentin in vitro. One hundred and ten dentin tubes prepared from bovine incisor teeth were infected in vitro for 14 days with Enterococcus faecalis. There...

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Veröffentlicht in:Australian endodontic journal 2012-04, Vol.38 (1), p.26-30
Hauptverfasser: Mohammadi, Zahed, Giardino, Luciano, Mombeinipour, Ali
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Giardino, Luciano
Mombeinipour, Ali
description The aim of this study was to evaluate the residual antibacterial activity of Tetraclean, MTAD and 5.25% sodium hypochlorite (NaOCl) in bovine root dentin in vitro. One hundred and ten dentin tubes prepared from bovine incisor teeth were infected in vitro for 14 days with Enterococcus faecalis. Thereafter, the specimens were divided into five groups as follows: Tetraclean; MTAD; 5.25% NaOCl; infected dentin tubes (positive control); and sterile dentin tubes (negative control). Dentin chips were collected using round burs into tryptic soy broth and after culturing, the number of colony‐forming units (CFU) was counted. The number of CFU in all experimental groups was minimum after treatment, and the results obtained were significantly different from each other at any time period (P 
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One hundred and ten dentin tubes prepared from bovine incisor teeth were infected in vitro for 14 days with Enterococcus faecalis. Thereafter, the specimens were divided into five groups as follows: Tetraclean; MTAD; 5.25% NaOCl; infected dentin tubes (positive control); and sterile dentin tubes (negative control). Dentin chips were collected using round burs into tryptic soy broth and after culturing, the number of colony‐forming units (CFU) was counted. The number of CFU in all experimental groups was minimum after treatment, and the results obtained were significantly different from each other at any time period (P &lt; 0.05). The Tetraclean group showed the most effective antibacterial action at all five experimental periods (P &lt; 0.05). MTAD group showed the least antibacterial activity after treatment. However, at days 7, 14, 21 and 28 MTAD showed more effective antibacterial action than NaOCl. In each group, the number of CFU increased significantly by time‐lapse (P &lt; 0.05). 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One hundred and ten dentin tubes prepared from bovine incisor teeth were infected in vitro for 14 days with Enterococcus faecalis. Thereafter, the specimens were divided into five groups as follows: Tetraclean; MTAD; 5.25% NaOCl; infected dentin tubes (positive control); and sterile dentin tubes (negative control). Dentin chips were collected using round burs into tryptic soy broth and after culturing, the number of colony‐forming units (CFU) was counted. The number of CFU in all experimental groups was minimum after treatment, and the results obtained were significantly different from each other at any time period (P &lt; 0.05). The Tetraclean group showed the most effective antibacterial action at all five experimental periods (P &lt; 0.05). MTAD group showed the least antibacterial activity after treatment. However, at days 7, 14, 21 and 28 MTAD showed more effective antibacterial action than NaOCl. In each group, the number of CFU increased significantly by time‐lapse (P &lt; 0.05). 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subjects Analysis of Variance
Animals
Anti-Bacterial Agents - pharmacology
Cattle
Colony Count, Microbial
Dental Pulp Cavity - microbiology
Dentin
Dentin - microbiology
Dentistry
doxycycline
Enterococcus faecalis
Enterococcus faecalis - drug effects
Enterococcus faecalis - isolation & purification
Food science
In Vitro Techniques
Microbiology
MTAD
Root Canal Irrigants - pharmacology
substantivity
Tetraclean
title Antibacterial substantivity of a new antibiotic-based endodontic irrigation solution
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