Monoallelic and Biallelic Deletions of 13q14.3 in Chronic Lymphocytic Leukemia: FISH vs miRNA RT-qPCR Detection
Deletion of 13q14.3 (del(13q)) is the most common cytogenetic abnormality in chronic lymphocytic leukemia (CLL) and implies a favorable prognosis. We explored the feasibility of detecting del(13q) by real-time quantitative polymerase chain reaction (PCR) for miR-15a and miR-16-1, whose loci are loca...
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| Veröffentlicht in: | American journal of clinical pathology 2012-04, Vol.137 (4), p.641-646 |
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| creator | SMONSKEY, Matthew T BLOCK, Annemarie W DEEB, George CHANAN-KHAN, Asher A BERNSTEIN, Zale P MILLER, Kena C WALLACE, Paul K STAROSTIK, Petr |
| description | Deletion of 13q14.3 (del(13q)) is the most common cytogenetic abnormality in chronic lymphocytic leukemia (CLL) and implies a favorable prognosis. We explored the feasibility of detecting del(13q) by real-time quantitative polymerase chain reaction (PCR) for miR-15a and miR-16-1, whose loci are located in the deleted region. We analyzed 23 cases of B-CLL with monoallelic (10 cases) or biallelic del(13q) (5 cases) and used trisomy 12-positive CLL samples (n = 8) as control samples. As expected, miR-15a was expressed at significantly lower levels in monoallelic del(13qx1) samples compared with trisomy 12 control samples (P = .001). Biallelic del(13q) (del(13qx2)) samples showed further reduction of miR-15a levels compared with monoallelic del(13q) (del(13qx1)) (P = .009). In contrast, miR-16-1 expression levels were generally much lower and variable, with the highest levels detected in del(13qx1). Analyzed retrospectively, miR-15a levels differ among the del(13q) groups. However, only del(13qx2) miR-15a levels are reduced enough to determine the allelic status of an individual sample prospectively by real-time quantitative PCR. |
| doi_str_mv | 10.1309/AJCPP31FSSRQTTAQ |
| format | Article |
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We explored the feasibility of detecting del(13q) by real-time quantitative polymerase chain reaction (PCR) for miR-15a and miR-16-1, whose loci are located in the deleted region. We analyzed 23 cases of B-CLL with monoallelic (10 cases) or biallelic del(13q) (5 cases) and used trisomy 12-positive CLL samples (n = 8) as control samples. As expected, miR-15a was expressed at significantly lower levels in monoallelic del(13qx1) samples compared with trisomy 12 control samples (P = .001). Biallelic del(13q) (del(13qx2)) samples showed further reduction of miR-15a levels compared with monoallelic del(13q) (del(13qx1)) (P = .009). In contrast, miR-16-1 expression levels were generally much lower and variable, with the highest levels detected in del(13qx1). Analyzed retrospectively, miR-15a levels differ among the del(13q) groups. However, only del(13qx2) miR-15a levels are reduced enough to determine the allelic status of an individual sample prospectively by real-time quantitative PCR.</description><identifier>ISSN: 0002-9173</identifier><identifier>EISSN: 1943-7722</identifier><identifier>DOI: 10.1309/AJCPP31FSSRQTTAQ</identifier><identifier>PMID: 22431542</identifier><identifier>CODEN: AJCPAI</identifier><language>eng</language><publisher>Chicago, IL: American Society of Clinical Pathologists</publisher><subject>Aged ; Aged, 80 and over ; Alleles ; Biological and medical sciences ; Chromosome Deletion ; Chromosome Disorders - genetics ; Chromosomes, Human, Pair 13 - genetics ; Female ; Hematologic and hematopoietic diseases ; Humans ; In Situ Hybridization, Fluorescence ; Investigative techniques, diagnostic techniques (general aspects) ; Leukemia, Lymphocytic, Chronic, B-Cell - genetics ; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis ; Male ; Medical sciences ; MicroRNAs - genetics ; Middle Aged ; Pathology. Cytology. Biochemistry. Spectrometry. 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We explored the feasibility of detecting del(13q) by real-time quantitative polymerase chain reaction (PCR) for miR-15a and miR-16-1, whose loci are located in the deleted region. We analyzed 23 cases of B-CLL with monoallelic (10 cases) or biallelic del(13q) (5 cases) and used trisomy 12-positive CLL samples (n = 8) as control samples. As expected, miR-15a was expressed at significantly lower levels in monoallelic del(13qx1) samples compared with trisomy 12 control samples (P = .001). Biallelic del(13q) (del(13qx2)) samples showed further reduction of miR-15a levels compared with monoallelic del(13q) (del(13qx1)) (P = .009). In contrast, miR-16-1 expression levels were generally much lower and variable, with the highest levels detected in del(13qx1). Analyzed retrospectively, miR-15a levels differ among the del(13q) groups. However, only del(13qx2) miR-15a levels are reduced enough to determine the allelic status of an individual sample prospectively by real-time quantitative PCR.</description><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Alleles</subject><subject>Biological and medical sciences</subject><subject>Chromosome Deletion</subject><subject>Chromosome Disorders - genetics</subject><subject>Chromosomes, Human, Pair 13 - genetics</subject><subject>Female</subject><subject>Hematologic and hematopoietic diseases</subject><subject>Humans</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Leukemia, Lymphocytic, Chronic, B-Cell - genetics</subject><subject>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</subject><subject>Male</subject><subject>Medical sciences</subject><subject>MicroRNAs - genetics</subject><subject>Middle Aged</subject><subject>Pathology. 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Miscellaneous investigative techniques</subject><subject>Real-Time Polymerase Chain Reaction</subject><issn>0002-9173</issn><issn>1943-7722</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpdkEtLw0AURgdRbK3uXcmAiKvUeeTpLkZrlaptGtdhMpnQqUkmzSRC_71TbBFc3e_yHQ6XC8AlRmNMUXAXvkbzOcWT5TJeJEm4OAJDHNjU8jxCjsEQIUSsAHt0AM60XiOEiY_sUzAgxKbYsckQqDdVK1aWopQcsjqHD_KwPYpSdFLVGqoCYrrB9phCWcNo1ara9LNt1awU33a7LPovUUl2Dycvyyn81rCS8XsI48TazKPYuDrBd7JzcFKwUouL_RyBz8lTEk2t2cfzSxTOrIa4fmf5OedMZDb1cheRnAcmc5NFToTv0sLlnOPMtBkrGBc2zYifE8ZpXhBWZD4dgdtfb9OqTS90l1ZSc1GWrBaq12lAAgfRALuGvP5HrlXf1ua4FCNkCOIHO9_VnuqzSuRp08qKtdv08EkD3OwBpjkri5bVXOo_znGx47ku_QG7KoLY</recordid><startdate>20120401</startdate><enddate>20120401</enddate><creator>SMONSKEY, Matthew T</creator><creator>BLOCK, Annemarie W</creator><creator>DEEB, George</creator><creator>CHANAN-KHAN, Asher A</creator><creator>BERNSTEIN, Zale P</creator><creator>MILLER, Kena C</creator><creator>WALLACE, Paul K</creator><creator>STAROSTIK, Petr</creator><general>American Society of Clinical Pathologists</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20120401</creationdate><title>Monoallelic and Biallelic Deletions of 13q14.3 in Chronic Lymphocytic Leukemia: FISH vs miRNA RT-qPCR Detection</title><author>SMONSKEY, Matthew T ; BLOCK, Annemarie W ; DEEB, George ; CHANAN-KHAN, Asher A ; BERNSTEIN, Zale P ; MILLER, Kena C ; WALLACE, Paul K ; STAROSTIK, Petr</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p268t-8dccaeb437d602dc9eb4c7d6ed2e863f6ccc1b37dbaface43b28d2ac3df2afb83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Alleles</topic><topic>Biological and medical sciences</topic><topic>Chromosome Deletion</topic><topic>Chromosome Disorders - genetics</topic><topic>Chromosomes, Human, Pair 13 - genetics</topic><topic>Female</topic><topic>Hematologic and hematopoietic diseases</topic><topic>Humans</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Leukemia, Lymphocytic, Chronic, B-Cell - genetics</topic><topic>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</topic><topic>Male</topic><topic>Medical sciences</topic><topic>MicroRNAs - genetics</topic><topic>Middle Aged</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. 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We explored the feasibility of detecting del(13q) by real-time quantitative polymerase chain reaction (PCR) for miR-15a and miR-16-1, whose loci are located in the deleted region. We analyzed 23 cases of B-CLL with monoallelic (10 cases) or biallelic del(13q) (5 cases) and used trisomy 12-positive CLL samples (n = 8) as control samples. As expected, miR-15a was expressed at significantly lower levels in monoallelic del(13qx1) samples compared with trisomy 12 control samples (P = .001). Biallelic del(13q) (del(13qx2)) samples showed further reduction of miR-15a levels compared with monoallelic del(13q) (del(13qx1)) (P = .009). In contrast, miR-16-1 expression levels were generally much lower and variable, with the highest levels detected in del(13qx1). Analyzed retrospectively, miR-15a levels differ among the del(13q) groups. However, only del(13qx2) miR-15a levels are reduced enough to determine the allelic status of an individual sample prospectively by real-time quantitative PCR.</abstract><cop>Chicago, IL</cop><pub>American Society of Clinical Pathologists</pub><pmid>22431542</pmid><doi>10.1309/AJCPP31FSSRQTTAQ</doi><tpages>6</tpages></addata></record> |
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| subjects | Aged Aged, 80 and over Alleles Biological and medical sciences Chromosome Deletion Chromosome Disorders - genetics Chromosomes, Human, Pair 13 - genetics Female Hematologic and hematopoietic diseases Humans In Situ Hybridization, Fluorescence Investigative techniques, diagnostic techniques (general aspects) Leukemia, Lymphocytic, Chronic, B-Cell - genetics Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis Male Medical sciences MicroRNAs - genetics Middle Aged Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Real-Time Polymerase Chain Reaction |
| title | Monoallelic and Biallelic Deletions of 13q14.3 in Chronic Lymphocytic Leukemia: FISH vs miRNA RT-qPCR Detection |
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