Angiotensin II-induced JNK activation is mediated by NAD(P)H oxidase in isolated rat pancreatic islets
Angiotensin II (AII), the active component of the renin angiotensin system (RAS), plays a vital role in the regulation of physiological processes of the cardiovascular system, but also has autocrine and paracrine actions in various tissues and organs. Many studies have shown the existence of RAS in...
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| Veröffentlicht in: | Regulatory peptides 2012-04, Vol.175 (1-3), p.1-6 |
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| creator | Alves, E.S. Haidar, A.A. Quadros, C.D. Carvalho, D.S. Morgan, D. Rocha, M.S. Curi, R. Carpinelli, A.R. Hirata, A.E. |
| description | Angiotensin II (AII), the active component of the renin angiotensin system (RAS), plays a vital role in the regulation of physiological processes of the cardiovascular system, but also has autocrine and paracrine actions in various tissues and organs. Many studies have shown the existence of RAS in the pancreas of humans and rodents. The aim of this study was to evaluate potential signaling pathways mediated by AII in isolated pancreatic islets of rats. Phosphorylation of MAPKs (ERK1/2, JNK and p38MAPK), and the interaction between proteins JAK/STAT were evaluated. AII increased JAK2/STAT1 (42%) and JAK2/STAT3 (100%) interaction without altering the total content of JAK2. Analyzing the activation of MAPKs (ERK1/2, JNK and p38MAPK) in isolated pancreatic islets from rats we observed that AII rapidly (3min) promoted a significant increase in the phosphorylation degree of these proteins after incubation with the hormone. Curiously JNK protein phosphorylation was inhibited by DPI, suggesting the involvement of NAD(P)H oxidase in the activation of protein.
► Angiotensin II (AII) mediates signaling pathways in the pancreas of human and rat. ► AII activates JAK2/STAT1,3 and MAPKs proteins such as JNK, ERK1/2 and p38MAPK. ► JNK protein phosphorylation is mediated by NAD(P)H oxidase enzyme. |
| doi_str_mv | 10.1016/j.regpep.2012.01.003 |
| format | Article |
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► Angiotensin II (AII) mediates signaling pathways in the pancreas of human and rat. ► AII activates JAK2/STAT1,3 and MAPKs proteins such as JNK, ERK1/2 and p38MAPK. ► JNK protein phosphorylation is mediated by NAD(P)H oxidase enzyme.</description><identifier>ISSN: 0167-0115</identifier><identifier>EISSN: 1873-1686</identifier><identifier>DOI: 10.1016/j.regpep.2012.01.003</identifier><identifier>PMID: 22280799</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Angiotensin II ; Angiotensin II - pharmacology ; Animals ; Apoptosis - drug effects ; Blotting, Western ; Female ; Islets of Langerhans - cytology ; Islets of Langerhans - drug effects ; Islets of Langerhans - metabolism ; Isolated islets ; JAK/STAT ; Janus Kinase 2 - metabolism ; MAPKs ; Mitogen-Activated Protein Kinase 1 - metabolism ; Mitogen-Activated Protein Kinase 3 - metabolism ; NADPH Oxidases - metabolism ; p38 Mitogen-Activated Protein Kinases - metabolism ; Phosphorylation - drug effects ; Rats ; Reactive Oxygen Species - metabolism ; Signal Transduction - drug effects ; STAT3 Transcription Factor - metabolism ; Vasoconstrictor Agents - pharmacology</subject><ispartof>Regulatory peptides, 2012-04, Vol.175 (1-3), p.1-6</ispartof><rights>2012 Elsevier B.V.</rights><rights>Copyright © 2012 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c407t-30bd8bfaca3236a3dfca326b307e24e9f4d33d75ed73d6053aa3eab59621c2923</citedby><cites>FETCH-LOGICAL-c407t-30bd8bfaca3236a3dfca326b307e24e9f4d33d75ed73d6053aa3eab59621c2923</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.regpep.2012.01.003$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22280799$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alves, E.S.</creatorcontrib><creatorcontrib>Haidar, A.A.</creatorcontrib><creatorcontrib>Quadros, C.D.</creatorcontrib><creatorcontrib>Carvalho, D.S.</creatorcontrib><creatorcontrib>Morgan, D.</creatorcontrib><creatorcontrib>Rocha, M.S.</creatorcontrib><creatorcontrib>Curi, R.</creatorcontrib><creatorcontrib>Carpinelli, A.R.</creatorcontrib><creatorcontrib>Hirata, A.E.</creatorcontrib><title>Angiotensin II-induced JNK activation is mediated by NAD(P)H oxidase in isolated rat pancreatic islets</title><title>Regulatory peptides</title><addtitle>Regul Pept</addtitle><description>Angiotensin II (AII), the active component of the renin angiotensin system (RAS), plays a vital role in the regulation of physiological processes of the cardiovascular system, but also has autocrine and paracrine actions in various tissues and organs. Many studies have shown the existence of RAS in the pancreas of humans and rodents. The aim of this study was to evaluate potential signaling pathways mediated by AII in isolated pancreatic islets of rats. Phosphorylation of MAPKs (ERK1/2, JNK and p38MAPK), and the interaction between proteins JAK/STAT were evaluated. AII increased JAK2/STAT1 (42%) and JAK2/STAT3 (100%) interaction without altering the total content of JAK2. Analyzing the activation of MAPKs (ERK1/2, JNK and p38MAPK) in isolated pancreatic islets from rats we observed that AII rapidly (3min) promoted a significant increase in the phosphorylation degree of these proteins after incubation with the hormone. Curiously JNK protein phosphorylation was inhibited by DPI, suggesting the involvement of NAD(P)H oxidase in the activation of protein.
► Angiotensin II (AII) mediates signaling pathways in the pancreas of human and rat. ► AII activates JAK2/STAT1,3 and MAPKs proteins such as JNK, ERK1/2 and p38MAPK. ► JNK protein phosphorylation is mediated by NAD(P)H oxidase enzyme.</description><subject>Angiotensin II</subject><subject>Angiotensin II - pharmacology</subject><subject>Animals</subject><subject>Apoptosis - drug effects</subject><subject>Blotting, Western</subject><subject>Female</subject><subject>Islets of Langerhans - cytology</subject><subject>Islets of Langerhans - drug effects</subject><subject>Islets of Langerhans - metabolism</subject><subject>Isolated islets</subject><subject>JAK/STAT</subject><subject>Janus Kinase 2 - metabolism</subject><subject>MAPKs</subject><subject>Mitogen-Activated Protein Kinase 1 - metabolism</subject><subject>Mitogen-Activated Protein Kinase 3 - metabolism</subject><subject>NADPH Oxidases - metabolism</subject><subject>p38 Mitogen-Activated Protein Kinases - metabolism</subject><subject>Phosphorylation - drug effects</subject><subject>Rats</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Signal Transduction - drug effects</subject><subject>STAT3 Transcription Factor - metabolism</subject><subject>Vasoconstrictor Agents - pharmacology</subject><issn>0167-0115</issn><issn>1873-1686</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtOwzAQRS0EglL4A4S8AxYJfjRxs0GqyqtQAQtYW449Qa7SJNhORf8elwJLVh55zp3RHIROKEkpofnlInXw3kGXMkJZSmhKCN9BAzoWPKH5ON9Fg4iJhFCaHaBD7xeE0EwIvo8OGGNjIopigKpJ827bAI23DZ7NEtuYXoPBD0-PWOlgVyrYtsHW4yUYq0JslWv8NLk-f7m4x-2nNcoDthuirb_bTgXcqUY7iFEd_2sI_gjtVar2cPzzDtHb7c3r9D6ZP9_NppN5okdEhIST0ozLSmnFGc8VN9WmyktOBLARFNXIcG5EBkZwk5OMK8VBlVmRM6pZwfgQnW3ndq796MEHubReQ12rBtrey4IVlLGMZ5EcbUntWu8dVLJzdqncWlIiN4LlQm4Fy41gSaiMgmPs9GdBX0Yjf6FfoxG42gIQz1xZcNJrC010ah3oIE1r_9_wBezrjhQ</recordid><startdate>20120410</startdate><enddate>20120410</enddate><creator>Alves, E.S.</creator><creator>Haidar, A.A.</creator><creator>Quadros, C.D.</creator><creator>Carvalho, D.S.</creator><creator>Morgan, D.</creator><creator>Rocha, M.S.</creator><creator>Curi, R.</creator><creator>Carpinelli, A.R.</creator><creator>Hirata, A.E.</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120410</creationdate><title>Angiotensin II-induced JNK activation is mediated by NAD(P)H oxidase in isolated rat pancreatic islets</title><author>Alves, E.S. ; Haidar, A.A. ; Quadros, C.D. ; Carvalho, D.S. ; Morgan, D. ; Rocha, M.S. ; Curi, R. ; Carpinelli, A.R. ; Hirata, A.E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c407t-30bd8bfaca3236a3dfca326b307e24e9f4d33d75ed73d6053aa3eab59621c2923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Angiotensin II</topic><topic>Angiotensin II - pharmacology</topic><topic>Animals</topic><topic>Apoptosis - drug effects</topic><topic>Blotting, Western</topic><topic>Female</topic><topic>Islets of Langerhans - cytology</topic><topic>Islets of Langerhans - drug effects</topic><topic>Islets of Langerhans - metabolism</topic><topic>Isolated islets</topic><topic>JAK/STAT</topic><topic>Janus Kinase 2 - metabolism</topic><topic>MAPKs</topic><topic>Mitogen-Activated Protein Kinase 1 - metabolism</topic><topic>Mitogen-Activated Protein Kinase 3 - metabolism</topic><topic>NADPH Oxidases - metabolism</topic><topic>p38 Mitogen-Activated Protein Kinases - metabolism</topic><topic>Phosphorylation - drug effects</topic><topic>Rats</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Signal Transduction - drug effects</topic><topic>STAT3 Transcription Factor - metabolism</topic><topic>Vasoconstrictor Agents - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alves, E.S.</creatorcontrib><creatorcontrib>Haidar, A.A.</creatorcontrib><creatorcontrib>Quadros, C.D.</creatorcontrib><creatorcontrib>Carvalho, D.S.</creatorcontrib><creatorcontrib>Morgan, D.</creatorcontrib><creatorcontrib>Rocha, M.S.</creatorcontrib><creatorcontrib>Curi, R.</creatorcontrib><creatorcontrib>Carpinelli, A.R.</creatorcontrib><creatorcontrib>Hirata, A.E.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Regulatory peptides</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alves, E.S.</au><au>Haidar, A.A.</au><au>Quadros, C.D.</au><au>Carvalho, D.S.</au><au>Morgan, D.</au><au>Rocha, M.S.</au><au>Curi, R.</au><au>Carpinelli, A.R.</au><au>Hirata, A.E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Angiotensin II-induced JNK activation is mediated by NAD(P)H oxidase in isolated rat pancreatic islets</atitle><jtitle>Regulatory peptides</jtitle><addtitle>Regul Pept</addtitle><date>2012-04-10</date><risdate>2012</risdate><volume>175</volume><issue>1-3</issue><spage>1</spage><epage>6</epage><pages>1-6</pages><issn>0167-0115</issn><eissn>1873-1686</eissn><abstract>Angiotensin II (AII), the active component of the renin angiotensin system (RAS), plays a vital role in the regulation of physiological processes of the cardiovascular system, but also has autocrine and paracrine actions in various tissues and organs. Many studies have shown the existence of RAS in the pancreas of humans and rodents. The aim of this study was to evaluate potential signaling pathways mediated by AII in isolated pancreatic islets of rats. Phosphorylation of MAPKs (ERK1/2, JNK and p38MAPK), and the interaction between proteins JAK/STAT were evaluated. AII increased JAK2/STAT1 (42%) and JAK2/STAT3 (100%) interaction without altering the total content of JAK2. Analyzing the activation of MAPKs (ERK1/2, JNK and p38MAPK) in isolated pancreatic islets from rats we observed that AII rapidly (3min) promoted a significant increase in the phosphorylation degree of these proteins after incubation with the hormone. Curiously JNK protein phosphorylation was inhibited by DPI, suggesting the involvement of NAD(P)H oxidase in the activation of protein.
► Angiotensin II (AII) mediates signaling pathways in the pancreas of human and rat. ► AII activates JAK2/STAT1,3 and MAPKs proteins such as JNK, ERK1/2 and p38MAPK. ► JNK protein phosphorylation is mediated by NAD(P)H oxidase enzyme.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>22280799</pmid><doi>10.1016/j.regpep.2012.01.003</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Angiotensin II Angiotensin II - pharmacology Animals Apoptosis - drug effects Blotting, Western Female Islets of Langerhans - cytology Islets of Langerhans - drug effects Islets of Langerhans - metabolism Isolated islets JAK/STAT Janus Kinase 2 - metabolism MAPKs Mitogen-Activated Protein Kinase 1 - metabolism Mitogen-Activated Protein Kinase 3 - metabolism NADPH Oxidases - metabolism p38 Mitogen-Activated Protein Kinases - metabolism Phosphorylation - drug effects Rats Reactive Oxygen Species - metabolism Signal Transduction - drug effects STAT3 Transcription Factor - metabolism Vasoconstrictor Agents - pharmacology |
| title | Angiotensin II-induced JNK activation is mediated by NAD(P)H oxidase in isolated rat pancreatic islets |
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