Molecular analysis of a novel FMRFamide-related peptide gene (SOFaRP2) and its expression pattern in the brain of the European cuttlefish Sepia officinalis
► We cloned a novel FMRFamide-related peptides (FaRPs) gene which encodes four FaRPs. ► All but TIFRFamide were found to induce chromatophore expansion in an in vitro assay. ► We found that SOFaRP2 gene is expressed in cuttlefish brain regions regulating chromatophore activities. FMRFamide-related p...
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| Veröffentlicht in: | Peptides (New York, N.Y. : 1980) N.Y. : 1980), 2012-03, Vol.34 (1), p.114-119 |
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| creator | Zhang, Zhuobin Goodwin, Eli Loi, Poh Kheng Tublitz, Nathan J. |
| description | ► We cloned a novel FMRFamide-related peptides (FaRPs) gene which encodes four FaRPs. ► All but TIFRFamide were found to induce chromatophore expansion in an in vitro assay. ► We found that SOFaRP2 gene is expressed in cuttlefish brain regions regulating chromatophore activities.
FMRFamide-related peptides (FaRPs) are among several neurotransmitters known to regulate the chromatophore function in the European cuttlefish Sepia officinalis. Here we report the cloning and sequencing of a novel S. officinalis FaRP gene (SOFaRP2). The complete 835-base pair cDNA sequence of the SOFaRP2 gene contains an open reading frame of 567 base pairs encoding 188 amino acids and four putative FaRPs, NSLFRFamide, GNLFRFamide, TIFRFamide and PHTPFRFamide. All except TIFRFamide cause chromatophore expansion when assayed in an in vitro chromatophore bioassay. To investigate the expression pattern of SOFaRP2 gene in the cuttlefish brain, in situ hybridization was performed using a full length RNA probe. The SOFaRP2 gene was expressed primarily in the posterior chromatophore, anterior chromatophore, lateral basal and optic lobes among other brain locations. The SOFaRP2 gene appears to be expressed in all brain regions involved in chromatophore regulation. These data suggests that some or all of the four FaRPs encoded by SOFaRP2 might be involved in controlling chromatophore activity in cuttlefish. |
| doi_str_mv | 10.1016/j.peptides.2011.07.011 |
| format | Article |
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FMRFamide-related peptides (FaRPs) are among several neurotransmitters known to regulate the chromatophore function in the European cuttlefish Sepia officinalis. Here we report the cloning and sequencing of a novel S. officinalis FaRP gene (SOFaRP2). The complete 835-base pair cDNA sequence of the SOFaRP2 gene contains an open reading frame of 567 base pairs encoding 188 amino acids and four putative FaRPs, NSLFRFamide, GNLFRFamide, TIFRFamide and PHTPFRFamide. All except TIFRFamide cause chromatophore expansion when assayed in an in vitro chromatophore bioassay. To investigate the expression pattern of SOFaRP2 gene in the cuttlefish brain, in situ hybridization was performed using a full length RNA probe. The SOFaRP2 gene was expressed primarily in the posterior chromatophore, anterior chromatophore, lateral basal and optic lobes among other brain locations. The SOFaRP2 gene appears to be expressed in all brain regions involved in chromatophore regulation. These data suggests that some or all of the four FaRPs encoded by SOFaRP2 might be involved in controlling chromatophore activity in cuttlefish.</description><identifier>ISSN: 0196-9781</identifier><identifier>EISSN: 1873-5169</identifier><identifier>DOI: 10.1016/j.peptides.2011.07.011</identifier><identifier>PMID: 21802466</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Amino acids ; Animals ; Base Sequence ; bioassays ; Brain ; Brain - metabolism ; Cephalopods ; Chromatophore ; Cloning ; complementary DNA ; Control ; Cuttlefish ; DNA, Complementary ; FMRFamide - chemistry ; FMRFamide-related peptides ; gene expression ; Genes ; In Situ Hybridization ; Molecular Sequence Data ; Neuropeptide ; Neuropeptides - chemistry ; Neuropeptides - genetics ; Neuropeptides - metabolism ; Neurotransmitters ; open reading frames ; Peptides ; RNA ; Sepia - genetics ; Sepia - metabolism ; Sepia officinalis ; Sequence Alignment ; Sequencing</subject><ispartof>Peptides (New York, N.Y. : 1980), 2012-03, Vol.34 (1), p.114-119</ispartof><rights>2011 Elsevier Inc.</rights><rights>Copyright © 2011 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c457t-746fd62dfa863be5a82c27ea843d38f9a6608b652008054c766082d450217ca23</citedby><cites>FETCH-LOGICAL-c457t-746fd62dfa863be5a82c27ea843d38f9a6608b652008054c766082d450217ca23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.peptides.2011.07.011$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27928,27929,45999</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21802466$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Zhuobin</creatorcontrib><creatorcontrib>Goodwin, Eli</creatorcontrib><creatorcontrib>Loi, Poh Kheng</creatorcontrib><creatorcontrib>Tublitz, Nathan J.</creatorcontrib><title>Molecular analysis of a novel FMRFamide-related peptide gene (SOFaRP2) and its expression pattern in the brain of the European cuttlefish Sepia officinalis</title><title>Peptides (New York, N.Y. : 1980)</title><addtitle>Peptides</addtitle><description>► We cloned a novel FMRFamide-related peptides (FaRPs) gene which encodes four FaRPs. ► All but TIFRFamide were found to induce chromatophore expansion in an in vitro assay. ► We found that SOFaRP2 gene is expressed in cuttlefish brain regions regulating chromatophore activities.
FMRFamide-related peptides (FaRPs) are among several neurotransmitters known to regulate the chromatophore function in the European cuttlefish Sepia officinalis. Here we report the cloning and sequencing of a novel S. officinalis FaRP gene (SOFaRP2). The complete 835-base pair cDNA sequence of the SOFaRP2 gene contains an open reading frame of 567 base pairs encoding 188 amino acids and four putative FaRPs, NSLFRFamide, GNLFRFamide, TIFRFamide and PHTPFRFamide. All except TIFRFamide cause chromatophore expansion when assayed in an in vitro chromatophore bioassay. To investigate the expression pattern of SOFaRP2 gene in the cuttlefish brain, in situ hybridization was performed using a full length RNA probe. The SOFaRP2 gene was expressed primarily in the posterior chromatophore, anterior chromatophore, lateral basal and optic lobes among other brain locations. The SOFaRP2 gene appears to be expressed in all brain regions involved in chromatophore regulation. These data suggests that some or all of the four FaRPs encoded by SOFaRP2 might be involved in controlling chromatophore activity in cuttlefish.</description><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>bioassays</subject><subject>Brain</subject><subject>Brain - metabolism</subject><subject>Cephalopods</subject><subject>Chromatophore</subject><subject>Cloning</subject><subject>complementary DNA</subject><subject>Control</subject><subject>Cuttlefish</subject><subject>DNA, Complementary</subject><subject>FMRFamide - chemistry</subject><subject>FMRFamide-related peptides</subject><subject>gene expression</subject><subject>Genes</subject><subject>In Situ Hybridization</subject><subject>Molecular Sequence Data</subject><subject>Neuropeptide</subject><subject>Neuropeptides - chemistry</subject><subject>Neuropeptides - genetics</subject><subject>Neuropeptides - metabolism</subject><subject>Neurotransmitters</subject><subject>open reading frames</subject><subject>Peptides</subject><subject>RNA</subject><subject>Sepia - genetics</subject><subject>Sepia - metabolism</subject><subject>Sepia officinalis</subject><subject>Sequence Alignment</subject><subject>Sequencing</subject><issn>0196-9781</issn><issn>1873-5169</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAURS0EotPCLxTvKIsMtpM4zg5UdQCpVVGHrq039kvrUSYOtlPRb-Fn62imLKk315bOu_fJl5BTzpaccfl5uxxxTM5iXArG-ZI1yyyvyIKrpixqLtvXZMF4K4u2UfyIHMe4ZYxVVavekiPBFROVlAvy98r3aKYeAoUB-sfoIvUdBTr4B-zp6upmBbscUwTsIaGlh1h6hwPSs_X1Cm5-ik952FKXIsU_Y8AYnR_oCClhGKgbaLpHugmQb9l7flxMwY8IAzVTSj12Lt7TNY4OMtA54_IqLr4jbzroI74_6Am5XV38Ov9eXF5_-3H-9bIwVd2koqlkZ6WwHShZbrAGJYxoEFRV2lJ1LUjJ1EbWgjHF6so081vYqmaCNwZEeUI-7n3H4H9PGJPeuWiw72FAP0XdipZnVKlMnv2X5E3JOFNt1hdRJkR2VHJG5R41wccYsNNjcDsIjxnSc9t6q5_b1nPbmjU6Sx48PWRMmx3af2PP9Wbgwx7owGu4Cy7q23V2kGw-bTlnf9kTmD_4wWHQ0TgcDFoX0CRtvXtpiyeI6Me0</recordid><startdate>20120301</startdate><enddate>20120301</enddate><creator>Zhang, Zhuobin</creator><creator>Goodwin, Eli</creator><creator>Loi, Poh Kheng</creator><creator>Tublitz, Nathan J.</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TN</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope><scope>7U5</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20120301</creationdate><title>Molecular analysis of a novel FMRFamide-related peptide gene (SOFaRP2) and its expression pattern in the brain of the European cuttlefish Sepia officinalis</title><author>Zhang, Zhuobin ; Goodwin, Eli ; Loi, Poh Kheng ; Tublitz, Nathan J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c457t-746fd62dfa863be5a82c27ea843d38f9a6608b652008054c766082d450217ca23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>bioassays</topic><topic>Brain</topic><topic>Brain - metabolism</topic><topic>Cephalopods</topic><topic>Chromatophore</topic><topic>Cloning</topic><topic>complementary DNA</topic><topic>Control</topic><topic>Cuttlefish</topic><topic>DNA, Complementary</topic><topic>FMRFamide - chemistry</topic><topic>FMRFamide-related peptides</topic><topic>gene expression</topic><topic>Genes</topic><topic>In Situ Hybridization</topic><topic>Molecular Sequence Data</topic><topic>Neuropeptide</topic><topic>Neuropeptides - chemistry</topic><topic>Neuropeptides - genetics</topic><topic>Neuropeptides - metabolism</topic><topic>Neurotransmitters</topic><topic>open reading frames</topic><topic>Peptides</topic><topic>RNA</topic><topic>Sepia - genetics</topic><topic>Sepia - metabolism</topic><topic>Sepia officinalis</topic><topic>Sequence Alignment</topic><topic>Sequencing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Zhuobin</creatorcontrib><creatorcontrib>Goodwin, Eli</creatorcontrib><creatorcontrib>Loi, Poh Kheng</creatorcontrib><creatorcontrib>Tublitz, Nathan J.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Peptides (New York, N.Y. : 1980)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Zhuobin</au><au>Goodwin, Eli</au><au>Loi, Poh Kheng</au><au>Tublitz, Nathan J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular analysis of a novel FMRFamide-related peptide gene (SOFaRP2) and its expression pattern in the brain of the European cuttlefish Sepia officinalis</atitle><jtitle>Peptides (New York, N.Y. : 1980)</jtitle><addtitle>Peptides</addtitle><date>2012-03-01</date><risdate>2012</risdate><volume>34</volume><issue>1</issue><spage>114</spage><epage>119</epage><pages>114-119</pages><issn>0196-9781</issn><eissn>1873-5169</eissn><abstract>► We cloned a novel FMRFamide-related peptides (FaRPs) gene which encodes four FaRPs. ► All but TIFRFamide were found to induce chromatophore expansion in an in vitro assay. ► We found that SOFaRP2 gene is expressed in cuttlefish brain regions regulating chromatophore activities.
FMRFamide-related peptides (FaRPs) are among several neurotransmitters known to regulate the chromatophore function in the European cuttlefish Sepia officinalis. Here we report the cloning and sequencing of a novel S. officinalis FaRP gene (SOFaRP2). The complete 835-base pair cDNA sequence of the SOFaRP2 gene contains an open reading frame of 567 base pairs encoding 188 amino acids and four putative FaRPs, NSLFRFamide, GNLFRFamide, TIFRFamide and PHTPFRFamide. All except TIFRFamide cause chromatophore expansion when assayed in an in vitro chromatophore bioassay. To investigate the expression pattern of SOFaRP2 gene in the cuttlefish brain, in situ hybridization was performed using a full length RNA probe. The SOFaRP2 gene was expressed primarily in the posterior chromatophore, anterior chromatophore, lateral basal and optic lobes among other brain locations. The SOFaRP2 gene appears to be expressed in all brain regions involved in chromatophore regulation. These data suggests that some or all of the four FaRPs encoded by SOFaRP2 might be involved in controlling chromatophore activity in cuttlefish.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>21802466</pmid><doi>10.1016/j.peptides.2011.07.011</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0196-9781 |
| ispartof | Peptides (New York, N.Y. : 1980), 2012-03, Vol.34 (1), p.114-119 |
| issn | 0196-9781 1873-5169 |
| language | eng |
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| subjects | Amino Acid Sequence Amino acids Animals Base Sequence bioassays Brain Brain - metabolism Cephalopods Chromatophore Cloning complementary DNA Control Cuttlefish DNA, Complementary FMRFamide - chemistry FMRFamide-related peptides gene expression Genes In Situ Hybridization Molecular Sequence Data Neuropeptide Neuropeptides - chemistry Neuropeptides - genetics Neuropeptides - metabolism Neurotransmitters open reading frames Peptides RNA Sepia - genetics Sepia - metabolism Sepia officinalis Sequence Alignment Sequencing |
| title | Molecular analysis of a novel FMRFamide-related peptide gene (SOFaRP2) and its expression pattern in the brain of the European cuttlefish Sepia officinalis |
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