First report on interferon related developmental regulator-1 from Macrobrachium rosenbergii: Bioinformatic analysis and gene expression
This study reports the first full length gene of interferon related developmental regulator-1 (designated as MrIRDR-1), identified from the transcriptome of Macrobrachium rosenbergii. The complete gene sequence of the MrIRDR-1 is 2459 base pair long with an open reading frame of 1308 base pairs and...
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| Veröffentlicht in: | Fish & shellfish immunology 2012-05, Vol.32 (5), p.929-933 |
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| container_title | Fish & shellfish immunology |
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| creator | Arockiaraj, Jesu Easwvaran, Sarasvathi Vanaraja, Puganeshwaran Singh, Arun Othman, Rofina Yasmin Bhassu, Subha |
| description | This study reports the first full length gene of interferon related developmental regulator-1 (designated as MrIRDR-1), identified from the transcriptome of Macrobrachium rosenbergii. The complete gene sequence of the MrIRDR-1 is 2459 base pair long with an open reading frame of 1308 base pairs and encoding a predicted protein of 436 amino acids with a calculated molecular mass of 48 kDa. The MrIRDR-1 protein contains a long interferon related developmental regulator super family domain between 30 and 330. The mRNA expressions of MrIRDR-1 in healthy and the infectious hypodermal and hematopoietic necrosis virus (IHHNV) infected M. rosenbergii were examined using qRT-PCR. The MrIRDR-1 is highly expressed in hepatopancreas along with all other tissues (walking leg, gills, muscle, haemocyte, pleopods, brain, stomach, intestine and eye stalk). After IHHNV infection, the expression is highly upregulated in hepatopancreas. This result indicates an important role of MrIRDR-1 in prawn defense system.
► MrIRDR-1 has been characterized bioinformatically. ► MrIRDR-1 contains a long IRDR super family domain. ► MrIRDR-1 is expressed in all the tissues and the highest at hepatopancreas. ► The expression is strongly upregulated after IHHNV infection. ► The result indicates the role of MrIRDR-1 in prawn defense system. |
| doi_str_mv | 10.1016/j.fsi.2012.02.011 |
| format | Article |
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► MrIRDR-1 has been characterized bioinformatically. ► MrIRDR-1 contains a long IRDR super family domain. ► MrIRDR-1 is expressed in all the tissues and the highest at hepatopancreas. ► The expression is strongly upregulated after IHHNV infection. ► The result indicates the role of MrIRDR-1 in prawn defense system.</description><identifier>ISSN: 1050-4648</identifier><identifier>EISSN: 1095-9947</identifier><identifier>DOI: 10.1016/j.fsi.2012.02.011</identifier><identifier>PMID: 22361112</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Amino Acid Sequence ; Animals ; Bioinformatic analysis ; Computational Biology ; Densovirinae - immunology ; DNA, Complementary - genetics ; DNA, Complementary - immunology ; Gene expression ; Gene Expression Profiling - veterinary ; Gene Expression Regulation ; Immediate-Early Proteins - chemistry ; Immediate-Early Proteins - genetics ; Immediate-Early Proteins - immunology ; Interferon ; Macrobrachium rosenbergii ; Molecular Sequence Data ; Open Reading Frames ; Palaemonidae - genetics ; Palaemonidae - immunology ; Phylogeny ; Real-Time Polymerase Chain Reaction - veterinary ; Sequence Alignment - veterinary ; Sequence Analysis, Protein - veterinary ; Sequence Homology ; Tissue Distribution ; Virus</subject><ispartof>Fish & shellfish immunology, 2012-05, Vol.32 (5), p.929-933</ispartof><rights>2012 Elsevier Ltd</rights><rights>Copyright © 2012 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-8f440d3b4174ae31aa0b16c8fd4899d6f1ef15d7500b8560f3cd5daf1290c7823</citedby><cites>FETCH-LOGICAL-c352t-8f440d3b4174ae31aa0b16c8fd4899d6f1ef15d7500b8560f3cd5daf1290c7823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1050464812000630$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22361112$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Arockiaraj, Jesu</creatorcontrib><creatorcontrib>Easwvaran, Sarasvathi</creatorcontrib><creatorcontrib>Vanaraja, Puganeshwaran</creatorcontrib><creatorcontrib>Singh, Arun</creatorcontrib><creatorcontrib>Othman, Rofina Yasmin</creatorcontrib><creatorcontrib>Bhassu, Subha</creatorcontrib><title>First report on interferon related developmental regulator-1 from Macrobrachium rosenbergii: Bioinformatic analysis and gene expression</title><title>Fish & shellfish immunology</title><addtitle>Fish Shellfish Immunol</addtitle><description>This study reports the first full length gene of interferon related developmental regulator-1 (designated as MrIRDR-1), identified from the transcriptome of Macrobrachium rosenbergii. The complete gene sequence of the MrIRDR-1 is 2459 base pair long with an open reading frame of 1308 base pairs and encoding a predicted protein of 436 amino acids with a calculated molecular mass of 48 kDa. The MrIRDR-1 protein contains a long interferon related developmental regulator super family domain between 30 and 330. The mRNA expressions of MrIRDR-1 in healthy and the infectious hypodermal and hematopoietic necrosis virus (IHHNV) infected M. rosenbergii were examined using qRT-PCR. The MrIRDR-1 is highly expressed in hepatopancreas along with all other tissues (walking leg, gills, muscle, haemocyte, pleopods, brain, stomach, intestine and eye stalk). After IHHNV infection, the expression is highly upregulated in hepatopancreas. This result indicates an important role of MrIRDR-1 in prawn defense system.
► MrIRDR-1 has been characterized bioinformatically. ► MrIRDR-1 contains a long IRDR super family domain. ► MrIRDR-1 is expressed in all the tissues and the highest at hepatopancreas. ► The expression is strongly upregulated after IHHNV infection. ► The result indicates the role of MrIRDR-1 in prawn defense system.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Bioinformatic analysis</subject><subject>Computational Biology</subject><subject>Densovirinae - immunology</subject><subject>DNA, Complementary - genetics</subject><subject>DNA, Complementary - immunology</subject><subject>Gene expression</subject><subject>Gene Expression Profiling - veterinary</subject><subject>Gene Expression Regulation</subject><subject>Immediate-Early Proteins - chemistry</subject><subject>Immediate-Early Proteins - genetics</subject><subject>Immediate-Early Proteins - immunology</subject><subject>Interferon</subject><subject>Macrobrachium rosenbergii</subject><subject>Molecular Sequence Data</subject><subject>Open Reading Frames</subject><subject>Palaemonidae - genetics</subject><subject>Palaemonidae - immunology</subject><subject>Phylogeny</subject><subject>Real-Time Polymerase Chain Reaction - veterinary</subject><subject>Sequence Alignment - veterinary</subject><subject>Sequence Analysis, Protein - veterinary</subject><subject>Sequence Homology</subject><subject>Tissue Distribution</subject><subject>Virus</subject><issn>1050-4648</issn><issn>1095-9947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UMtuFDEQHCEiEgIfwAX5xmkWt-exYziRiDykRLnA2fLY7cWrGXtoeyLyBfw2Xm3giFRSl0rVJVVV1TvgG-DQf9xvXPIbwUFseAHAi-oMuOxqKdvtywPveN327XBavU5pzznvm56_qk6FaHoAEGfV7ytPKTPCJVJmMTAfMpJDKpRw0hkts_iIU1xmDFlPRd2tRY9UA3MUZ3avDcWRtPnh15lRTBhGpJ33n9iFjz64SLPO3jAd9PSUfCrEsh0GZPhrIUzJx_CmOnF6Svj2-Z5X36--fru8qe8erm8vv9zVpulErgfXttw2YwvbVmMDWvMRejM42w5S2t4BOujstuN8HLqeu8bYzmoHQnKzHURzXn045i4Uf66Yspp9MjhNOmBck5JCgoCmk8UJR2dplxKhUwv5WdOTAq4O86u9KvOrw_yKFwCUn_fP6es4o_338XfvYvh8NGDp-OiRVDIeg0HrCU1WNvr_xP8BJwiYyw</recordid><startdate>20120501</startdate><enddate>20120501</enddate><creator>Arockiaraj, Jesu</creator><creator>Easwvaran, Sarasvathi</creator><creator>Vanaraja, Puganeshwaran</creator><creator>Singh, Arun</creator><creator>Othman, Rofina Yasmin</creator><creator>Bhassu, Subha</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120501</creationdate><title>First report on interferon related developmental regulator-1 from Macrobrachium rosenbergii: Bioinformatic analysis and gene expression</title><author>Arockiaraj, Jesu ; Easwvaran, Sarasvathi ; Vanaraja, Puganeshwaran ; Singh, Arun ; Othman, Rofina Yasmin ; Bhassu, Subha</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-8f440d3b4174ae31aa0b16c8fd4899d6f1ef15d7500b8560f3cd5daf1290c7823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Bioinformatic analysis</topic><topic>Computational Biology</topic><topic>Densovirinae - immunology</topic><topic>DNA, Complementary - genetics</topic><topic>DNA, Complementary - immunology</topic><topic>Gene expression</topic><topic>Gene Expression Profiling - veterinary</topic><topic>Gene Expression Regulation</topic><topic>Immediate-Early Proteins - chemistry</topic><topic>Immediate-Early Proteins - genetics</topic><topic>Immediate-Early Proteins - immunology</topic><topic>Interferon</topic><topic>Macrobrachium rosenbergii</topic><topic>Molecular Sequence Data</topic><topic>Open Reading Frames</topic><topic>Palaemonidae - genetics</topic><topic>Palaemonidae - immunology</topic><topic>Phylogeny</topic><topic>Real-Time Polymerase Chain Reaction - veterinary</topic><topic>Sequence Alignment - veterinary</topic><topic>Sequence Analysis, Protein - veterinary</topic><topic>Sequence Homology</topic><topic>Tissue Distribution</topic><topic>Virus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Arockiaraj, Jesu</creatorcontrib><creatorcontrib>Easwvaran, Sarasvathi</creatorcontrib><creatorcontrib>Vanaraja, Puganeshwaran</creatorcontrib><creatorcontrib>Singh, Arun</creatorcontrib><creatorcontrib>Othman, Rofina Yasmin</creatorcontrib><creatorcontrib>Bhassu, Subha</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fish & shellfish immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Arockiaraj, Jesu</au><au>Easwvaran, Sarasvathi</au><au>Vanaraja, Puganeshwaran</au><au>Singh, Arun</au><au>Othman, Rofina Yasmin</au><au>Bhassu, Subha</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>First report on interferon related developmental regulator-1 from Macrobrachium rosenbergii: Bioinformatic analysis and gene expression</atitle><jtitle>Fish & shellfish immunology</jtitle><addtitle>Fish Shellfish Immunol</addtitle><date>2012-05-01</date><risdate>2012</risdate><volume>32</volume><issue>5</issue><spage>929</spage><epage>933</epage><pages>929-933</pages><issn>1050-4648</issn><eissn>1095-9947</eissn><abstract>This study reports the first full length gene of interferon related developmental regulator-1 (designated as MrIRDR-1), identified from the transcriptome of Macrobrachium rosenbergii. The complete gene sequence of the MrIRDR-1 is 2459 base pair long with an open reading frame of 1308 base pairs and encoding a predicted protein of 436 amino acids with a calculated molecular mass of 48 kDa. The MrIRDR-1 protein contains a long interferon related developmental regulator super family domain between 30 and 330. The mRNA expressions of MrIRDR-1 in healthy and the infectious hypodermal and hematopoietic necrosis virus (IHHNV) infected M. rosenbergii were examined using qRT-PCR. The MrIRDR-1 is highly expressed in hepatopancreas along with all other tissues (walking leg, gills, muscle, haemocyte, pleopods, brain, stomach, intestine and eye stalk). After IHHNV infection, the expression is highly upregulated in hepatopancreas. This result indicates an important role of MrIRDR-1 in prawn defense system.
► MrIRDR-1 has been characterized bioinformatically. ► MrIRDR-1 contains a long IRDR super family domain. ► MrIRDR-1 is expressed in all the tissues and the highest at hepatopancreas. ► The expression is strongly upregulated after IHHNV infection. ► The result indicates the role of MrIRDR-1 in prawn defense system.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>22361112</pmid><doi>10.1016/j.fsi.2012.02.011</doi><tpages>5</tpages></addata></record> |
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| subjects | Amino Acid Sequence Animals Bioinformatic analysis Computational Biology Densovirinae - immunology DNA, Complementary - genetics DNA, Complementary - immunology Gene expression Gene Expression Profiling - veterinary Gene Expression Regulation Immediate-Early Proteins - chemistry Immediate-Early Proteins - genetics Immediate-Early Proteins - immunology Interferon Macrobrachium rosenbergii Molecular Sequence Data Open Reading Frames Palaemonidae - genetics Palaemonidae - immunology Phylogeny Real-Time Polymerase Chain Reaction - veterinary Sequence Alignment - veterinary Sequence Analysis, Protein - veterinary Sequence Homology Tissue Distribution Virus |
| title | First report on interferon related developmental regulator-1 from Macrobrachium rosenbergii: Bioinformatic analysis and gene expression |
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