All 4 Bile Salt Hydrolase Proteins Are Responsible for the Hydrolysis Activity in Lactobacillus plantarum ST-III
: In vertebrates, bile salt hydrolysis plays an essential role in fat metabolism. Bile salts are synthesized in the liver. And in the small intestine glycine and taurine are de‐conjugated from bile salts by the enzyme bile salt hydrolase (BSH) from intestinal microbes. However, the mechanism of bil...
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| Veröffentlicht in: | Journal of food science 2011-11, Vol.76 (9), p.M622-M628 |
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| creator | Ren, Jing Sun, Kejie Wu, Zhengjun Yao, Jing Guo, Benheng |
| description | : In vertebrates, bile salt hydrolysis plays an essential role in fat metabolism. Bile salts are synthesized in the liver. And in the small intestine glycine and taurine are de‐conjugated from bile salts by the enzyme bile salt hydrolase (BSH) from intestinal microbes. However, the mechanism of bile salt hydrolysis in Lactobacillus plantarum is still ambiguous. Four predicted bile salt hydrolase (bsh) genes from L. plantarum ST‐III were cloned into Escherichia coli. The function of these genes was explored by overexpression. All 4 proteins that were studied showed activity against glycine‐ or taurine‐conjugated bile salts. Substrate preference was also observed in BSH proteins, especially for the enzyme BSH1, which had high hydrolysis activity for glycodeoxycholic acid. These results suggest that all 4 bile salt hydrolases may be responsible for the bile salt hydrolysis activity in L. plantarum ST‐III.
Practical Application: Hypercholesterolemia is considered one of the major risk factors for coronary heart disease. More interest has focused on intestinal microbes because of their role in the decrease of serum cholesterol. BSH proteins play an important role in the reduction of cholesterol. This paper adds to a better understanding of BSH proteins of intestinal microbes. It gives a great hint that probiotics can be used to solve hypercholesterolemia one day. |
| doi_str_mv | 10.1111/j.1750-3841.2011.02431.x |
| format | Article |
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Practical Application: Hypercholesterolemia is considered one of the major risk factors for coronary heart disease. More interest has focused on intestinal microbes because of their role in the decrease of serum cholesterol. BSH proteins play an important role in the reduction of cholesterol. This paper adds to a better understanding of BSH proteins of intestinal microbes. It gives a great hint that probiotics can be used to solve hypercholesterolemia one day.</description><identifier>ISSN: 0022-1147</identifier><identifier>EISSN: 1750-3841</identifier><identifier>DOI: 10.1111/j.1750-3841.2011.02431.x</identifier><identifier>PMID: 22416715</identifier><identifier>CODEN: JFDSAZ</identifier><language>eng</language><publisher>Malden, USA: Blackwell Publishing Inc</publisher><subject>Amidohydrolases - genetics ; Amidohydrolases - metabolism ; Amino Acid Sequence ; Bile ; Bile Acids and Salts - metabolism ; Biological and medical sciences ; Chromatography, High Pressure Liquid ; Cloning, Molecular ; enzyme ; Escherichia coli ; Escherichia coli - genetics ; Escherichia coli - metabolism ; Food industries ; Food microbiology ; Food science ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Gene Expression Regulation, Bacterial ; Genes, Bacterial ; Gram-positive bacteria ; Hydrolysis ; Lactobacillus ; Lactobacillus plantarum - enzymology ; Lactobacillus plantarum - genetics ; Metabolism ; Molecular Sequence Data ; probiotics ; Proteins</subject><ispartof>Journal of food science, 2011-11, Vol.76 (9), p.M622-M628</ispartof><rights>2011 Institute of Food Technologists</rights><rights>2015 INIST-CNRS</rights><rights>Copyright Institute of Food Technologists Nov/Dec 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5631-1ac188489f273b25094ae27dba8e9337722a88f7db59837d80919fca601ad3103</citedby><cites>FETCH-LOGICAL-c5631-1ac188489f273b25094ae27dba8e9337722a88f7db59837d80919fca601ad3103</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1750-3841.2011.02431.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1750-3841.2011.02431.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25228716$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22416715$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ren, Jing</creatorcontrib><creatorcontrib>Sun, Kejie</creatorcontrib><creatorcontrib>Wu, Zhengjun</creatorcontrib><creatorcontrib>Yao, Jing</creatorcontrib><creatorcontrib>Guo, Benheng</creatorcontrib><title>All 4 Bile Salt Hydrolase Proteins Are Responsible for the Hydrolysis Activity in Lactobacillus plantarum ST-III</title><title>Journal of food science</title><addtitle>J Food Sci</addtitle><description>: In vertebrates, bile salt hydrolysis plays an essential role in fat metabolism. Bile salts are synthesized in the liver. And in the small intestine glycine and taurine are de‐conjugated from bile salts by the enzyme bile salt hydrolase (BSH) from intestinal microbes. However, the mechanism of bile salt hydrolysis in Lactobacillus plantarum is still ambiguous. Four predicted bile salt hydrolase (bsh) genes from L. plantarum ST‐III were cloned into Escherichia coli. The function of these genes was explored by overexpression. All 4 proteins that were studied showed activity against glycine‐ or taurine‐conjugated bile salts. Substrate preference was also observed in BSH proteins, especially for the enzyme BSH1, which had high hydrolysis activity for glycodeoxycholic acid. These results suggest that all 4 bile salt hydrolases may be responsible for the bile salt hydrolysis activity in L. plantarum ST‐III.
Practical Application: Hypercholesterolemia is considered one of the major risk factors for coronary heart disease. More interest has focused on intestinal microbes because of their role in the decrease of serum cholesterol. BSH proteins play an important role in the reduction of cholesterol. This paper adds to a better understanding of BSH proteins of intestinal microbes. It gives a great hint that probiotics can be used to solve hypercholesterolemia one day.</description><subject>Amidohydrolases - genetics</subject><subject>Amidohydrolases - metabolism</subject><subject>Amino Acid Sequence</subject><subject>Bile</subject><subject>Bile Acids and Salts - metabolism</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Cloning, Molecular</subject><subject>enzyme</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - metabolism</subject><subject>Food industries</subject><subject>Food microbiology</subject><subject>Food science</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genes, Bacterial</subject><subject>Gram-positive bacteria</subject><subject>Hydrolysis</subject><subject>Lactobacillus</subject><subject>Lactobacillus plantarum - enzymology</subject><subject>Lactobacillus plantarum - genetics</subject><subject>Metabolism</subject><subject>Molecular Sequence Data</subject><subject>probiotics</subject><subject>Proteins</subject><issn>0022-1147</issn><issn>1750-3841</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkV1v0zAUhiMEYmXwF5CFhOAmwceOY_sGqQy2FVUD0aJdWk7qCBc3CXayNf8eh5YicYHwjb-e8-rYT5IgwBnE8WabAWc4pSKHjGCADJOcQrZ_kMxOFw-TGcaEpAA5P0uehLDF054Wj5MzQnIoOLBZ0s2dQzl6Z51BK-16dD1ufOt0MOizb3tjm4Dm3qAvJnRtE2wZubr1qP9mjugYbESq3t7ZfkS2QUtd9W2pK-vcEFDndNNrP-zQap0uFounyaNau2CeHefz5Ovlh_XFdbr8dLW4mC_TihUUUtAVCJELWRNOS8KwzLUhfFNqYSSlnBOihajjAZOC8o3AEmRd6QKD3lDA9Dx5dcjtfPtjMKFXOxsq42I7ph2CkkRILAtMI_n6nyRgguN_Assj-uIvdNsOvonvUBIXhEvIWYTEAap8G4I3teq83Wk_xiQ16VNbNVlSkyU16VO_9Kl9LH1-zB_KndmcCn_7isDLI6BDpV3tdVPZ8IdjhAgOReTeHrj7KHb87wbUx8v3q2kZA9JDgA292Z8CtP-uCk45U7c3Vwqz9Q1b3UoF9CcZWcI6</recordid><startdate>201111</startdate><enddate>201111</enddate><creator>Ren, Jing</creator><creator>Sun, Kejie</creator><creator>Wu, Zhengjun</creator><creator>Yao, Jing</creator><creator>Guo, Benheng</creator><general>Blackwell Publishing Inc</general><general>Wiley</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QR</scope><scope>7ST</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7QL</scope><scope>7X8</scope></search><sort><creationdate>201111</creationdate><title>All 4 Bile Salt Hydrolase Proteins Are Responsible for the Hydrolysis Activity in Lactobacillus plantarum ST-III</title><author>Ren, Jing ; Sun, Kejie ; Wu, Zhengjun ; Yao, Jing ; Guo, Benheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5631-1ac188489f273b25094ae27dba8e9337722a88f7db59837d80919fca601ad3103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Amidohydrolases - genetics</topic><topic>Amidohydrolases - metabolism</topic><topic>Amino Acid Sequence</topic><topic>Bile</topic><topic>Bile Acids and Salts - metabolism</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Cloning, Molecular</topic><topic>enzyme</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - metabolism</topic><topic>Food industries</topic><topic>Food microbiology</topic><topic>Food science</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Genes, Bacterial</topic><topic>Gram-positive bacteria</topic><topic>Hydrolysis</topic><topic>Lactobacillus</topic><topic>Lactobacillus plantarum - enzymology</topic><topic>Lactobacillus plantarum - genetics</topic><topic>Metabolism</topic><topic>Molecular Sequence Data</topic><topic>probiotics</topic><topic>Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ren, Jing</creatorcontrib><creatorcontrib>Sun, Kejie</creatorcontrib><creatorcontrib>Wu, Zhengjun</creatorcontrib><creatorcontrib>Yao, Jing</creatorcontrib><creatorcontrib>Guo, Benheng</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of food science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ren, Jing</au><au>Sun, Kejie</au><au>Wu, Zhengjun</au><au>Yao, Jing</au><au>Guo, Benheng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>All 4 Bile Salt Hydrolase Proteins Are Responsible for the Hydrolysis Activity in Lactobacillus plantarum ST-III</atitle><jtitle>Journal of food science</jtitle><addtitle>J Food Sci</addtitle><date>2011-11</date><risdate>2011</risdate><volume>76</volume><issue>9</issue><spage>M622</spage><epage>M628</epage><pages>M622-M628</pages><issn>0022-1147</issn><eissn>1750-3841</eissn><coden>JFDSAZ</coden><abstract>: In vertebrates, bile salt hydrolysis plays an essential role in fat metabolism. Bile salts are synthesized in the liver. And in the small intestine glycine and taurine are de‐conjugated from bile salts by the enzyme bile salt hydrolase (BSH) from intestinal microbes. However, the mechanism of bile salt hydrolysis in Lactobacillus plantarum is still ambiguous. Four predicted bile salt hydrolase (bsh) genes from L. plantarum ST‐III were cloned into Escherichia coli. The function of these genes was explored by overexpression. All 4 proteins that were studied showed activity against glycine‐ or taurine‐conjugated bile salts. Substrate preference was also observed in BSH proteins, especially for the enzyme BSH1, which had high hydrolysis activity for glycodeoxycholic acid. These results suggest that all 4 bile salt hydrolases may be responsible for the bile salt hydrolysis activity in L. plantarum ST‐III.
Practical Application: Hypercholesterolemia is considered one of the major risk factors for coronary heart disease. More interest has focused on intestinal microbes because of their role in the decrease of serum cholesterol. BSH proteins play an important role in the reduction of cholesterol. This paper adds to a better understanding of BSH proteins of intestinal microbes. It gives a great hint that probiotics can be used to solve hypercholesterolemia one day.</abstract><cop>Malden, USA</cop><pub>Blackwell Publishing Inc</pub><pmid>22416715</pmid><doi>10.1111/j.1750-3841.2011.02431.x</doi><tpages>7</tpages></addata></record> |
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| subjects | Amidohydrolases - genetics Amidohydrolases - metabolism Amino Acid Sequence Bile Bile Acids and Salts - metabolism Biological and medical sciences Chromatography, High Pressure Liquid Cloning, Molecular enzyme Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Food industries Food microbiology Food science Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation, Bacterial Genes, Bacterial Gram-positive bacteria Hydrolysis Lactobacillus Lactobacillus plantarum - enzymology Lactobacillus plantarum - genetics Metabolism Molecular Sequence Data probiotics Proteins |
| title | All 4 Bile Salt Hydrolase Proteins Are Responsible for the Hydrolysis Activity in Lactobacillus plantarum ST-III |
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